Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,561,765	T→C	C47C (TGT→TGC)	yffN →	CPZ‑55 prophage; uncharacterized protein YffN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,561,765	0	T	C	100.0%	280.0 / NA	79	C47C (TGT→TGC)	yffN	CPZ‑55 prophage; uncharacterized protein YffN
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (43/36);  total (43/36)

CGTTTTTAAATATCTTGATTTTGCAGAAGACCGCGAACACGCAGAATCAGTGGCGACCAAAGAGTTAAAACTGGACCACGTAGAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGTGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTCAGATCTATGAGGCCGGAGGCGATGGAATAGAGCCACTCAATTGTGGAATCGAATCAAGGAAGGTGGCAATTGCTGCCCTGCTTCGCGTCATGCGT  >  NC_000913/2561633‑2561900                                                                                                                                     |                                                                                                                                        cGTTTTTAAATATCTTGATTTTGCAGAAGACCGCGAACACGCAGAATCAGTGGCGACCAAAGAGTTAAAACTGGACCACGTAGAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGc                                                                                                                                  <  2:929638/140‑1 (MQ=255)   tttttAAATATCTTGATTTTGCAGAAGACCGCGAACACGCAGAATCAGTGGCGACCAAAGAGTTAAAACTGGACCACGTAGAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCtt                                                                                                                                >  2:113741/1‑140 (MQ=255)            atCTTGATTTTGCAGAAGACCGCGAACACGCAGAATCAGTTGCGACCAAAGAGTTAAAACTGGACCACGTAGAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAg                                                                                                                                   <  1:1034745/128‑1 (MQ=255)            atCTTGATTTTGCAGAAGACCGCGAACACGCAGAATCAGTTGCGACCAAAGAGTTAAAACTGGACCACGTAGAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAg                                                                                                                                   >  2:1034745/1‑128 (MQ=255)              cTTGATTTTGCAGAAGACCGCGAACACGCAGAATCAGTGGCGACCAAAGAGTTAAAACTGGACCACGTAGAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGGGCTTGTCCAGCCtgg                                                                                                                     >  1:539312/1‑140 (MQ=255)              cTTGATTTTGCAGAAGACCGCGAACACGCAGAATCAGTGGCGACCAAAGAGTTAAAACTGGACCACGTAGAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGt                                                                                                                              <  2:1435334/131‑1 (MQ=255)              cTTGATTTTGCAGAAGACCGCGAACACGCAGAATCAGTGGCGACCAAAGAGTTAAAACTGGACCACGTAGAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGt                                                                                                                              >  1:1435334/1‑131 (MQ=255)              cTTGATTTTGCAGAAGACCGCGAACACGCAGAATCAGTGGCGACCAAAGAGTTAAAACTGGACCACGTAGAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCtgg                                                                                                                     >  1:96491/1‑140 (MQ=255)              cTTGATTTTGCAGAAGACCGCGAACACGCAGAATCAGTGGCGACCAAAGAGTTAAAACTGGACCACGTAGAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCtgg                                                                                                                     >  2:1509701/1‑140 (MQ=255)              cTTGATTTTGCAGAAGACCGCGAACACGCAGAATCAGTGGCGACCAAAGAGTTAAAACTGGACCACGTAGAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCtgg                                                                                                                     >  1:1439302/1‑140 (MQ=255)                tGATTTTGCAGAAGACCGCGAACACGCATAATCAGTGGCGACCAAAGAGTTAAAACTGGACCACGTAGAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCtggtg                                                                                                                   >  1:1318183/1‑140 (MQ=255)                     ttGCAGAAGACCGCGAACACGCAGAATCAGTGGCGACCAAAGAGTTAAAACTGGACCACGTAGAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCtg                                                                                                                      <  2:220802/132‑1 (MQ=255)                     ttGCAGAAGACCGCGAACACGCAGAATCAGTGGCGACCAAAGAGTTAAAACTGGACCACGTAGAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCtg                                                                                                                      >  1:220802/1‑132 (MQ=255)                       gCAGAAGACCGCGAACACGCAGAATCAGTGGCGACCAAAGAGTTAAAACTGGACCACGTAGAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGa                                                                                                            <  2:96491/140‑1 (MQ=255)                           aagaCCGCGAACACGAAGAATCAGTGGCGACCAAAGATTTAAAACTGGACCACGTAGAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAg                                                                                                        <  1:682872/140‑1 (MQ=255)                            agaCCGCGAACACGCAGAATCAGTGGCGACCAAAGAGTTAAAACTGGACCACGTAGAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGc                                                                                                       <  2:872413/140‑1 (MQ=255)                            agaCCGCGAACACGCAGAATCAGTGGCGACCAAAGAGTTAAAACTGGACCACGTAGAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGc                                                                                                       <  1:1493642/140‑1 (MQ=255)                            agaCCGCGAACACGCAGAATCAGTGGCGACCAAAGAGTTAAAACTGGACCACGTAGAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGc                                                                                                       <  1:821747/140‑1 (MQ=255)                              aCCGCGAACACGCAGAATCAGTGGCGACCAAAGAGTTAAAACTGGACCACGTAGAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTg                                                                                                     <  2:1439302/140‑1 (MQ=255)                                cgcgAACACGCATAATCAGTGGCGACCAAAGAGTTAAAACTGGACCACGTAGAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGt                                                                                                   <  2:1318183/140‑1 (MQ=255)                                cgcgAACACGCAGAATCAGTGGCGACCAAAGAGTTAAAACTGGACCACGTAGAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGt                                                                                                   >  1:1119561/1‑140 (MQ=255)                                cgcgAACACGCAGAATCAGTGGCGACCAAAGAGTTAAAACTGGACCACGTAGAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGt                                                                                                   >  1:210890/1‑140 (MQ=255)                                cgcgAACACGCAGAATCAGTGGCGACCAAAGAGTTAAAACTGGACCACGTAGAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGt                                                                                                   >  1:35287/1‑140 (MQ=255)                                 gcgAACACGCAGAATCAGTGGCGACCAAAGAGTTAAAACTGGACCACGTAGAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGtt                                                                                                  <  1:113741/140‑1 (MQ=255)                                  cgAACACGCAGAATCAGTGGCGACCAAAGAGTTAAAACTGGACCACGTAGAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTc                                                                                                 >  1:1148284/1‑140 (MQ=255)                                  cgAACACGCAGAATCAGTGGCGACCAAAGAGTTAAAACTGGACCACGTAGAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTc                                                                                                 >  1:824069/1‑140 (MQ=255)                                  cgAACACGCAGAATCAGTGGCGACCAAAGAGTTAAAACTGGACCACGTAGAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCCTTGAGCTGGTCCAGCCAGGTGGATTCGATGAGCTGGTTc                                                                                                 >  2:700764/1‑140 (MQ=255)                                   gAACACGCAGAATCAGTGGCGACCAAAGAGTCAAAGCTGAACCACGTATAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTCa                                                                                                <  2:539312/140‑1 (MQ=255)                                             aaTCAGTGGCGACCAAAGAGTTAAAACTGGACCACGTAGAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTCAGATCTATGAg                                                                                      >  2:235448/1‑140 (MQ=255)                                             aaTCAGTGGCGACCAAAGAGTTAAAACTGGACCACGTAGAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTCAGATCTATGAg                                                                                      >  1:203825/1‑140 (MQ=255)                                               tCAGTGGCGACCAAAGAGTTAAAACTGGACCACGTAGAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGTTTCGATAAGCTGGATCAGATCTATGAGGc                                                                                    >  1:668292/1‑140 (MQ=255)                                                  gTGGCGACCAAAGAGTTAAAACTGGACCACGTAGAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTCAGATCTATg                                                                                        >  2:1234331/1‑133 (MQ=255)                                                  gTGGCGACCAAAGAGTTAAAACTGGACCACGTAGAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTCAGATCTATg                                                                                        <  1:1234331/133‑1 (MQ=255)                                                  gTGGCGACCAAAGAGTTAAAACTGGACCACGTAGAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTCAGATCTATg                                                                                        >  2:900508/1‑133 (MQ=255)                                                  gTGGCGACCAAAGAGTTAAAACTGGACCACGTAGAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTCAGATCTATg                                                                                        <  1:900508/133‑1 (MQ=255)                                                   tGGCGACCAAAGAGTTAAAACTGGACCACGTAGAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTCAGATCTATGAGGCCGGa                                                                                <  2:1394002/140‑1 (MQ=255)                                                   tGGCGACCAAAGAGTTAAAACTGGACCACGTAGAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTCAGATCTATGAGGCCGGa                                                                                <  2:35287/140‑1 (MQ=255)                                                   tGGCGACCAAAGAGTTAAAACTGGACCACGTAGAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTCAGATCTATGAGGCCGGa                                                                                >  2:1007645/1‑140 (MQ=255)                                                     gCGACCAAAGAGTTAAAACTGGACCACGTAGAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTCAGATCTATGAGGCCGGAgg                                                                              <  1:1259604/140‑1 (MQ=255)                                                     gCGACCAAAGAGTTAAAACTGGACCACGTAGAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTCAGATCTATGAGGCCGGAgg                                                                              <  1:1509701/140‑1 (MQ=255)                                                                gTTAAAACTGGACCCCGTAGAACAAATCGGTATCCGCTATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTCAGATCTATGAGGCCGGAGGCGATGGAATag                                                                   <  1:494004/140‑1 (MQ=255)                                                                gTTAAAACTGGACCACGTAGAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTCAGATCTATGAGGCCGGAGGCGATGGAATag                                                                   <  1:1460739/140‑1 (MQ=255)                                                                gTTAAAACTGGACCACGTAGAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTCAGATCTATGAGGCCGGAGGCGATGGAATag                                                                   <  1:1547996/140‑1 (MQ=255)                                                                  tAAAACTGGACCACGCAGAAAACTTCGCTGTCCGCGAACTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTCAGATCTATGAGGCCGGAGGCGATGGAATagag                                                                 <  1:700764/140‑1 (MQ=255)                                                                         ggACCACGTAGAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTGAGATCTACGAGGCCGGGGGCGGTGGAATAGAGCCACTCa                                                          >  2:494004/1‑140 (MQ=255)                                                                         ggACCACGTAGAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTCAGATCTATGAGGCCGGAGGCGATGGAATAGAGCCACTCa                                                          >  2:921067/1‑140 (MQ=255)                                                                          gACCACGTAGAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTCAGATCTATGAGGCCGGAGGCGa                                                                           >  2:1081873/1‑122 (MQ=255)                                                                          gACCACGTAGAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTCAGATCTATGAGGCCGGAGGCGa                                                                           <  1:1081873/122‑1 (MQ=255)                                                                           aCCACGTAGAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTCAGATCTATGAGGCCGGAGGCGATGGAATAGAGCCACTCAAt                                                        >  2:177831/1‑140 (MQ=255)                                                                               cGTAGAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTCAGATCTATGAGGCCGGAGGCGATGGAATAGAGCCACTCTAttcca                                                    >  2:190271/1‑137 (MQ=255)                                                                               cGTAGAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTCAGATCTAGGAGGCCGGAGGAGATGGAATAGAGCCACTCAATtgtg                                                    >  1:570049/1‑140 (MQ=255)                                                                                  aGAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTCAGATCTATGAGGCCGGAGGCGATGGAATAGAGCCACTCAATTGTGgaa                                                 <  2:824069/140‑1 (MQ=255)                                                                                   gAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTCAGATCTATGAGGCCGGAGGCGATGGAATAGAGCCACTCAATTGTGgaat                                                <  1:1440923/140‑1 (MQ=255)                                                                                   gAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTCAGATCTATGAGGCCGGAGGCGATGGAATAGAGCCACTCAATTGTGgaat                                                <  1:1509101/140‑1 (MQ=255)                                                                                   gAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTCAGATCTATGAGGCCGGAGGCGATGGAATAGAGCCACTCAATTGTGgaat                                                <  1:823454/140‑1 (MQ=255)                                                                                           cGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTCAGATCTATTAGGCCGGAGGCGATGGAATAGAGCCGCTCAATTGTGGAATCGAATCaa                                        >  2:628530/1‑140 (MQ=255)                                                                                                     gATCTAGCCAATGATCTCAAAGGGCGTGGGTGCGTTGGGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTCAGATCTATGAGGCCGGAGGCGATGGAATAGAGCCACTCAATTGTGGAATCGAATCAAGGAAGGTGGc                              <  1:697281/140‑1 (MQ=255)                                                                                                     gATCTAGCCAATGATATCAAAGAGCGTGGGCGCGTTGAGCTTGTCCAGCCTGGTGAATTCGATGAGCTGGTTCAGATCTATGAGGCCGGAGGCGATGGAATAGAGCCACTCAATTGTGGAATCGAATCAAGGAAGGTGGc                              <  1:1463948/140‑1 (MQ=255)                                                                                                      aTCTAGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGTCTGGTGGATTCGATGAGCTGGTTCAGATCTGTGAGGCAGGAGACGATGGAATAGAGCCACTCAATTGTGGAATCGAATGAAGAAAGGTGGCa                             >  2:955554/1‑140 (MQ=255)                                                                                                          aggaaaTGATATCCAAGAGCGTGGGTGCGTTGAGATTGTCCAGCCTGGTGGATTCGATGAGCTGGTTCAGATCTATGAGGCCGGAGGCGATGGAATAGAGCCACTCAATTGTGGAATCGAATCAAGGAAGGTGGCAATtg                         <  1:955554/136‑1 (MQ=255)                                                                                                          aGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTCAGATCTATGAGGCCGGAGGCGATGGAATAGAGCCACTCAATTGTGGAATCGAATCAAGGAAGGTGGCAATtg                         <  1:921067/140‑1 (MQ=255)                                                                                                          aGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTCAGATCTATGAGGCCGGAGGCGATGGAATAGAGCCACTCAATTGTGGAATCGAATCAAGGAAGGTGGCAATtg                         <  1:177831/140‑1 (MQ=255)                                                                                                          aGCCAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTCAGATCTATGAGGCCGGAGGCGATGGAATAGAGCCACTCAATTGTGGAATCGAATCAAGGAAGGTGGCAATtg                         <  2:1119561/140‑1 (MQ=255)                                                                                                            ccAATGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTCAGATCTATGAGGCCGGAGGCGATGGAATAGAGCCACTCAATTGTGGAATCGAATCAAGGAAGGTGGCAATtgct                       >  2:1543970/1‑140 (MQ=255)                                                                                                               aTGATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTCAGATCTATGAGGCCGGAGGCGATGGAATAGAGCCACTCAATTGTGGAATCGAATCAAGGAAGGTGGCAATTGCTGcc                    <  1:235448/140‑1 (MQ=255)                                                                                                                 gATATCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTCAGATCTATGAGGCCGGAGGCGATGGAATAGCGCCACTCACTTGGGTAATCGAATCAGGGAAATTGGCAATGGCTGcccc                  >  2:697281/1‑139 (MQ=255)                                                                                                                     tCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTCAGATCTATGAGGCCGGAGGCGATGGAATAGAGCCACTCAATTGTGGAATCGAATCAAGGAAGGTGGCAATTGCTGCCCTGCtt              >  2:161660/1‑140 (MQ=255)                                                                                                                     tCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTCAGATCTATGAGGCCGGAGGCGATGGAATAGAGCCACTCAATTGTGGAATCGAATCAAGGAAGGTGGCAATTGCTGCCCTGCtt              >  2:817682/1‑140 (MQ=255)                                                                                                                     tCAAAGAGCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTCAGATCTATGAGGCCGGAGGCGATGGAATAGAGCCACTCAATTGTGGAATCGAATCAAGGAAGGTGGCAATTGCTGCCCTGCtt              >  2:817553/1‑140 (MQ=255)                                                                                                                            gCGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTCAGATCTATGAGGCCGGAGGCGATGGAATAGAGCCACTCAATTGTGGAATCGAATCAAGGAAGGTTGCAATTGCTGCCCTGCTTCGCGTCa       >  2:1216999/1‑140 (MQ=255)                                                                                                                             cGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTCAGATCTATGAGGCCGGAGGCGATGGAATAGAGCGACTCAATTGTGGAATCGAATCAAGGAAGGTGGCAATTGCTGCCCTGCTTCGCGTCAt      >  2:922830/1‑140 (MQ=255)                                                                                                                             cGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTCAGATCTATGAGGCCGGAGGCGATGGAATAGAGCCACTCAATTTTGGAATCGGATCGAGGTAGGTGGCAGTTGCGGCCCTGCTTCGCGTCAt      >  2:433309/1‑140 (MQ=255)                                                                                                                             cGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTCAGATCTATGAGGCCGGAGGCGATGGAATAGAGCCACTCAATTGTGGAATCGAATCAAGGAAGGTGGCAATTGCTGCCCTGCTTCGCGTCAt      >  2:770319/1‑140 (MQ=255)                                                                                                                             cGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTCAGATCTATGAGGCCGGAGGCGATGGAATAGAGCCACTCAATTGTGGAATCGAATCAAGGAAGGTGGCAATTGCTGCCCTGCTTCGCGTCAt      >  2:225617/1‑140 (MQ=255)                                                                                                                             cGTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTCAGATCTATGAGGCCGGAGGCGATGGAATAGAGCCACTCAATTGTGGAATCGAATCAAGGAAGGTGGCAATTGCTGCCCTGCTTCGCGTCAt      >  1:1472862/1‑140 (MQ=255)                                                                                                                              gTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTCAGATCTATGAGGCCGGAGGCGATGGAATAGAGCCACTCAATTGTGGAATCGAATCAAGGAAGGTGGCAATtgctgc                     >  2:41538/1‑124 (MQ=255)                                                                                                                              gTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTCAGATCTATGAGGCCGGAGGCGATGGAATAGAGCCACTCAATTGTGGAATCGAATCAAGGAAGGTGGCAATtgctgc                     <  1:41538/124‑1 (MQ=255)                                                                                                                              gTGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTCAGATCTATGAGGCCGGAGGCGATGGAATAGAGCCACTCAATTGTGGAATCGAATAAAGGAAGGTGGGAATTGCTGCCCTGCTTCGCGTCATg     >  1:676137/1‑140 (MQ=255)                                                                                                                               tGGGTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTCAGATCTATGAGGCCGGAGGCGATGGAATAGAGCCACTCAATTGTGGAATCGAACCAAGGTACGTGGCAATTGCTGGCCTGCTTCTCGTCATGc    >  2:686330/1‑140 (MQ=255)                                                                                                                                 ggTGCGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTCAGATCTATGAGGCCGGAGGCGATGGAATAGAGCCACTCAATTGTGGAATCGAATCAAGGAAGGTGGCAATTGCTGCCCTGCTTCGCGTCATGCGt  <  1:1007645/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                        CGTTTTTAAATATCTTGATTTTGCAGAAGACCGCGAACACGCAGAATCAGTGGCGACCAAAGAGTTAAAACTGGACCACGTAGAAAAATTCGCTATCCGCGATCTAGCCAATGATATCAAAGAGCGTGGGTGTGTTGAGCTTGTCCAGCCTGGTGGATTCGATGAGCTGGTTCAGATCTATGAGGCCGGAGGCGATGGAATAGAGCCACTCAATTGTGGAATCGAATCAAGGAAGGTGGCAATTGCTGCCCTGCTTCGCGTCATGCGT  >  NC_000913/2561633‑2561900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑