Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,576,882	A→G	V499A (GTC→GCC)	maeB ←	malate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,576,882	0	A	G	100.0%	328.0 / NA	93	V499A (GTC→GCC)	maeB	malate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (51/42);  total (51/42)

GAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGACGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGGACGACCGATAAGGATCGGTTTCGCCAGTCCCAGCGTTACCAGTTCCTGAGTGGCATGCAGAACGCG  >  NC_000913/2576745‑2577018                                                                                                                                          |                                                                                                                                         gAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCg                                                                                                                                        <  1:850092/140‑1 (MQ=255)  aaCCATGATCGCGCCGATCACTGTCGGGTTACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGa                                                                                                                                       <  1:39209/140‑1 (MQ=255)  aaCCATGATCGCGCCGATCACTGTCGGGTTACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGa                                                                                                                                       >  1:1468610/1‑140 (MQ=255)  aaCCATGATCGCGCCGATCACTGTCGGGTTACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAATACTCTTTAAATCGCGGATCGCATTCGTTATTGGCGa                                                                                                                                       >  1:640748/1‑140 (MQ=255)    ccATGATCGCGCCGATCACTGTCGGGTTACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGAtc                                                                                                                                     <  2:214959/140‑1 (MQ=255)            gcgcCGATCACTGTCGGGTTACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGTTCGCATACGTTATTGGCGATCTCAAAATc                                                                                                                             >  1:706686/1‑140 (MQ=255)            gcgcCGATCACTGTCGGGTTACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTGAAACCGCAGTTCGGATTCGTTTTTGGCGATCTCAAAATc                                                                                                                             >  2:475875/1‑140 (MQ=255)            gcgcCGATCACTGTCGGGTTACTGATCAGCGCCCGCTGCGCCTGTTCCCGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATc                                                                                                                             >  2:1069165/1‑140 (MQ=255)                cGATCACTGTCGGGTTACTGATCAGGGCCGGCTGCGCTTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACg                                                                                                                         <  1:750942/140‑1 (MQ=255)                cGATCACTGTCGGGTTACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACg                                                                                                                         >  2:1351367/1‑140 (MQ=255)                 gATCACTGTCGGGTTACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGc                                                                                                                        <  2:1095903/140‑1 (MQ=255)                 gATCACTGTCGGGTTACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGc                                                                                                                        <  2:184285/140‑1 (MQ=255)                   tCACTGTCGGGTTACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGccc                                                                                                                      >  1:757657/1‑140 (MQ=255)                      cTGTCGGGTTACTGATCAGCGCCCGCTGCGCCTGTTCCCGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCt                                                                                                                   <  1:1069165/140‑1 (MQ=255)                         tCGGGTTACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTg                                                                                                                <  2:1468610/140‑1 (MQ=255)                          cGGGTTACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGa                                                                                                               <  1:1351367/140‑1 (MQ=255)                               tACTGATCGGCGCCCGGTGCGCATGTTCCTAGGCGCCGCTGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGc                                                                                                          <  2:737437/140‑1 (MQ=255)                                 cTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATGGGATTCGTCATTAGCGGTCTCAAAAGCAACGCCCGCTTTGATCTGCaa                                                                                                        >  2:449631/1‑140 (MQ=255)                                  tGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAg                                                                                                       <  2:1042085/140‑1 (MQ=255)                                    gtCAGCGCCCGCTGCGCTTGTGTCTGAGGGCAGCCTCGACGCTTCATGGTCGGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGcc                                                                                                     <  2:706686/139‑1 (MQ=255)                                    aTCAGCGCCCGCTGCGCGTGTTCCTGAGTGATGCCGCGATGCTTCATGTTCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGcc                                                                                                     <  2:640748/140‑1 (MQ=255)                                    aTCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGcc                                                                                                     <  2:144709/140‑1 (MQ=255)                                       aGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGcc                                                                                                     <  2:156233/137‑1 (MQ=255)                                       aGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGcc                                                                                                     >  1:156233/1‑137 (MQ=255)                                        gcgcCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGt                                                                                                 >  2:316581/1‑140 (MQ=255)                                         cgcCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGATATTGGCGATCTTAGAATCAACGCCTGCTTATATCTGCAGGCCCAGtt                                                                                                >  2:723492/1‑140 (MQ=255)                                           cccGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTc                                                                                              >  1:989926/1‑140 (MQ=255)                                                tGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAAt                                                                                         >  2:1502643/1‑140 (MQ=255)                                                tGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAAt                                                                                         >  2:1156987/1‑140 (MQ=255)                                                   gcCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATgcg                                                                                      <  1:303033/140‑1 (MQ=255)                                                        ttCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCAttt                                                                                 >  2:317159/1‑140 (MQ=255)                                                           cTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGa                                                                              <  1:316581/140‑1 (MQ=255)                                                                   cgccgcGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTc                                                                      >  1:99099/1‑140 (MQ=255)                                                                   cgccgcGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTc                                                                      >  1:103027/1‑140 (MQ=255)                                                                    gccgcGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCg                                                                     >  1:869955/1‑140 (MQ=255)                                                                     ccgcGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCgg                                                                    >  1:239006/1‑140 (MQ=255)                                                                      cgcgACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGga                                                                   <  1:223907/140‑1 (MQ=255)                                                                       gcgACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGgac                                                                  <  1:1156987/140‑1 (MQ=255)                                                                        cgACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGgacg                                                                 >  2:390238/1‑140 (MQ=255)                                                                         gACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGgacga                                                                <  2:99278/140‑1 (MQ=255)                                                                         caggCTTCATGATCTGGTAGTACTCGGTCCACTACTCTTCAAAGCGCGCATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGgacga                                                                <  2:395974/137‑1 (MQ=255)                                                                          aCGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGgacgac                                                               >  1:350035/1‑140 (MQ=255)                                                                             cTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGGACGACCGa                                                            <  2:103027/140‑1 (MQ=255)                                                                                cATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCCAGCCCAGTGTCTGAATTCGCATTTCTATCACGTTCGGACTACCGATaa                                                         >  2:695054/1‑140 (MQ=255)                                                                                     tCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGGACGACCGATAAGGATc                                                    >  1:203512/1‑140 (MQ=255)                                                                                       tGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGGGCATTTCGATCACGTTCGGACGACCGATAAGGATctg                                                  >  1:395974/1‑138 (MQ=255)                                                                                       tGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGGACGACCGATAAGGATCgg                                                  >  2:1460483/1‑140 (MQ=255)                                                                                        ggAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGGACGACCGATAAGGATCGGt                                                 >  1:1049766/1‑140 (MQ=255)                                                                                        ggAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGATTCTGAATGCGCCTATAGATCACGTGCGGAAGACCGATATGGATCGGt                                                 >  2:706372/1‑140 (MQ=255)                                                                                           aGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGGACGACCGATAAGGATCGGTTTc                                              >  2:1458644/1‑140 (MQ=255)                                                                                              aTTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGGACGACCGATAAGGATCGGTTTCGcc                                           >  1:1500005/1‑140 (MQ=255)                                                                                               ttCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGGACGACCGATAAGGATCGGTTTCGCCa                                          >  2:189067/1‑140 (MQ=255)                                                                                               ttCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACATTCGGACGACCGATAAGGATCGGTTTCGCCa                                          >  2:1409047/1‑140 (MQ=255)                                                                                                 cGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGGACGACCGATAAGGATCGGTTTCGCCAGt                                        <  2:869955/140‑1 (MQ=255)                                                                                                 cGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATCCGCATTTCCATCACGTTCGGACGACGGATAAGGATCGGTTTCGCCAGt                                        >  2:540835/1‑140 (MQ=255)                                                                                                  ggTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACATTCGGACGACCGATAAGGATCGGTTTCGCCAGTc                                       <  1:1409047/140‑1 (MQ=255)                                                                                                   gTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGGACGACCGATAAGGATCGGt                                                 >  2:68076/1‑129 (MQ=255)                                                                                                   gTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGGACGACCGATAAGGATCGGt                                                 <  1:68076/129‑1 (MQ=255)                                                                                                    tCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGGATGACCGAAAAGGATCGGTTTCGCCAGTccc                                     >  1:578705/1‑140 (MQ=255)                                                                                                    tCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGGACGACCGATAAGGATCGGTTTCGCCAGTccc                                     <  1:317159/140‑1 (MQ=255)                                                                                                     ccAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGGACGACCGATAAGGATCGGTTTCGCCAGTCCCa                                    <  1:1502643/140‑1 (MQ=255)                                                                                                       aGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTACGGACGAACGAGAAGGATCGGTTTCGCCAGTCCCAGc                                  >  1:468302/1‑140 (MQ=255)                                                                                                        gttctctTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGGACGACCGATAAGGATCGGTGTCGCCAGTCCCAGCg                                 <  2:1260550/137‑1 (MQ=255)                                                                                                        gTACTCTTTAAAGCGCGGATCGCATCCGTTATTGGCGCTCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGGACGACCGATAAGGATCGGTTTCGCCAGTCCCAGCg                                 <  1:449631/140‑1 (MQ=255)                                                                                                          aCTCTTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGGACGACCGATAAGGATCGGTTTCGCCAGTCCCAGCGtt                               <  1:1208587/140‑1 (MQ=255)                                                                                                            tctTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGGACGACCGATAAGGATCGGTTTCGCCAGTCCCAGCGTTAc                             <  2:1500005/140‑1 (MQ=255)                                                                                                            tctTTAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGGACGACCGATAAGGATCGGTTTCGCCAGTCCCAGCGTTAc                             <  1:1458644/140‑1 (MQ=255)                                                                                                              tttAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGGACGACCGATAATGATTGGGTTCGCCCGTCCCAGCGTTACCa                           >  1:550524/1‑140 (MQ=255)                                                                                                              tttAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGGACGACCGATAAGGATGGGGTTCGCCAGTCCCAGCGTTACCa                           >  1:403579/1‑140 (MQ=255)                                                                                                              tttAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGGACGACCGATAAGGATCGGTTTTGCCAGTCCCAGCGTTACCa                           >  2:1193597/1‑140 (MQ=255)                                                                                                              tttAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGGACGACCGATAAGGATCGGTTTCGCCAGTCCCAGCGTTACCa                           >  1:142199/1‑140 (MQ=255)                                                                                                               ttAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGGACGACCGATAAGGATCGGTTTCGCCAGTCCCAGCGTTACCAg                          <  1:189067/140‑1 (MQ=255)                                                                                                               ttAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGGACGACCGATAAGGATCGGTTTCGCCAGTCCCAGCGTTACCAg                          <  1:1460483/140‑1 (MQ=255)                                                                                                               ttAAAGCGCGGATCGGACTCGTAATTGGCGATCTCGAAATCAACGCCCACTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGGACGACCGATAAGGATCGGTTTCGCCAGTCCCAGCGTTACCAg                          <  2:468302/140‑1 (MQ=255)                                                                                                                tAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGGACGACCGATAAGGATCGGTTTCGCCAGTCCCAGCGTTACCAGt                         >  1:370529/1‑140 (MQ=255)                                                                                                                tAAAGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGGACGACCGATAAGGATCGGTTTCGCCAGTCCCAGCGTTACCAGt                         >  2:977116/1‑140 (MQ=255)                                                                                                                   aGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGGACGACCGATAAGGATCGGTTTCGCCAGTCCCAGCGTTACCAGTTcc                      >  1:1175313/1‑140 (MQ=255)                                                                                                                   aGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGGACGACCGATAAGGATCGGTTTCGCCAGTCCCAGCGTTACCAGTTcc                      >  2:1443417/1‑140 (MQ=255)                                                                                                                   aGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGGACGACCGATAAGGATCGGTTTCGCCAGTCCCAGCGTTACCAGTTcc                      >  2:871560/1‑140 (MQ=255)                                                                                                                   aGCGCGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGGACGACCGAGAAGGATCAGTTGCGGCAGTCCGAGCGTTACCAGTTcc                      >  1:454245/1‑140 (MQ=255)                                                                                                                    gcgcGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTACAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGGACGACCGATAAGGATCGGTTTCGCCAGTc                                       <  1:1125663/122‑1 (MQ=255)                                                                                                                    gcgcGGATCGGATTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTACAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGGACGACCGATAAGGATCGGTTTCGCCAGTc                                       >  2:1125663/1‑122 (MQ=255)                                                                                                                        ggatcggatTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGGACTACCGATAACGATTGGTATCGCCAGTCCCAGCGTTACCAGTTCCTGAGt                 >  2:516031/1‑140 (MQ=255)                                                                                                                        ggatcggatTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGGACGACCGATAAGGATCGGTTTTGCCAGTCCCAGCGTTACCAGTTCCTGAGt                 <  1:1193597/140‑1 (MQ=255)                                                                                                                         gatcggatTCGTTATCGGCGATATCGAAACCAACGCCTGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGGACGACCGATAAGGATCGGTTTCGCCAGTCCCAGCGTTACCAGTTCCTGAGTg                <  1:723492/140‑1 (MQ=255)                                                                                                                          atcggatTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGGACGACCGATAAGGATCGGTTTCGCCAGTCCCAGCGTTAcc                            >  1:1002748/1‑127 (MQ=255)                                                                                                                          atcggatTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGGACGACCGATAAGGATCGGTTTCGCCAGTCCCAGCGTTAcc                            <  2:1535872/127‑1 (MQ=255)                                                                                                                          atcggatTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGGACGACCGATAAGGATCGGTTTCGCCAGTCCCAGCGTTAcc                            >  1:1535872/1‑127 (MQ=255)                                                                                                                          atcggatTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGGACGACCGATAAGGATCGGTTTCGCCAGTCCCAGCGTTAcc                            <  2:1002748/127‑1 (MQ=255)                                                                                                                           tcggatTCGTTATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGGACGACCGATAAGGATCGGTTTCGCCAGTCCCAGCGTTACCAGTTCCTGAGTGGc              <  1:977116/140‑1 (MQ=255)                                                                                                                                    ttATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGGACGACCGATAAGGATCGGTTTCGCCGGTCCCAGGGTTGCCGGTTCCTGAGTGGCATGCAGAAc     >  1:697719/1‑140 (MQ=255)                                                                                                                                    ttATTGGCGATCTCAAAATCAACACCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGGACGACCGATAAGGATCGGTTTCGCCAGTCCCAGCGTTACCAGTTCCTGAGTGGCATGCAGAAc     >  1:17484/1‑140 (MQ=255)                                                                                                                                     tATTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGGACGACCGATAAGGATCGGTTTCGCCAGTCCCAGCGTTACCAGTTCCTGAGTGGCATGCAGAAcg    <  2:370529/140‑1 (MQ=255)                                                                                                                                      aTTGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGGACGACCGATAAGGATCGGTTTCGCCAGTCCCAGCGTTACCAGTTCCTGAGTGGCATGCAGAAcgc   >  1:104885/1‑140 (MQ=255)                                                                                                                                       ttGGCGATCTCAAAATCAACGCGCGATTTTATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGGACGACCGATAAGGATCGGTTTCGCCAGTCCCAGCGTTACCAGTTCCTGAGTGGCATGCAGAAcgcg  <  1:706372/140‑1 (MQ=255)                                                                                                                                       ttGGCGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGGACGACCGATAAGGATCGGTTTCGCCAGTCCCAGCGTTACCAGTTCCTGAGTGGCATGCAGAAcgcg  <  2:142199/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         GAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGACGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCCAGTTTCTGAATGCGCATTTCGATCACGTTCGGACGACCGATAAGGATCGGTTTCGCCAGTCCCAGCGTTACCAGTTCCTGAGTGGCATGCAGAACGCG  >  NC_000913/2576745‑2577018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑