BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,906,811	G→A	D377D (GAC→GAT)	eno ←	enolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,906,811	0	G	A	99.0%	348.4 / NA	105	D377D (GAC→GAT)	eno	enolase
Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (61/41); total (61/44)
Fisher's exact test for biased strand distribution p-value = 4.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 109 total aligned reads displayed.

TTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCGTCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTT  >  NC_000913/2906674‑2906946

ttGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATc                                                                                                                                       >  1:101455/1‑140 (MQ=255)

ttGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATc                                                                                                                                       >  2:940283/1‑140 (MQ=255)

ttGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATc                                                                                                                                       >  2:1246105/1‑140 (MQ=255)

ttGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTGGATCTGGCGTGCGGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATc                                                                                                                                       >  2:397045/1‑140 (MQ=255)

  gTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCtt                                                                                                                                     >  1:218646/1‑140 (MQ=255)

  gTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCtt                                                                                                                                     <  2:957637/140‑1 (MQ=255)

      ggTGCTTTTTCGCCCCGAGCTTCTTCGATTCGAATCACCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGt                                                                                                                                 <  1:423210/140‑1 (MQ=255)

      ggTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGt                                                                                                                                 <  1:352062/140‑1 (MQ=255)

      ggTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGt                                                                                                                                 <  1:1055370/140‑1 (MQ=255)

        tGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGttt                                                                                                                               <  2:797946/140‑1 (MQ=255)

         gCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTc                                                                                                                              >  1:762211/1‑140 (MQ=255)

          cTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCTGGTGAGGGAT‑GGTCGCATCTTCAGTTTCg                                                                                                                             >  1:567536/1‑140 (MQ=255)

          cTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCg                                                                                                                             >  2:1332899/1‑140 (MQ=255)

          cTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCg                                                                                                                             >  2:1523112/1‑140 (MQ=255)

          cTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCAGCAGCAGTTCCATCAGGCAGGTCAGTGAT‑GTTAGCATCTTCAGTTTCg                                                                                                                             >  2:440154/1‑140 (MQ=255)

          cTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGAGCTGGCCTGCAGCAGTACCAACACCCAGGTCAGCTAT‑GGTAGCATCTTCAGTTTCg                                                                                                                             >  2:368184/1‑140 (MQ=255)

           tttttCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGc                                                                                                                            >  2:36292/1‑140 (MQ=255)

             tttCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCa                                                                                                                          >  2:807175/1‑140 (MQ=255)

                    cAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGt                                                                                                                   >  2:1469482/1‑140 (MQ=255)

                      gagCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTga                                                                                                                 <  1:1523112/140‑1 (MQ=255)

                             ttcGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATaa                                                                                                          <  2:302651/140‑1 (MQ=255)

                                 aTACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGc                                                                                                      >  1:125169/1‑140 (MQ=255)

                                 aTACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGc                                                                                                      >  1:270130/1‑140 (MQ=255)

                                   aCGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAg                                                                                                    >  2:1287612/1‑140 (MQ=255)

                                   aCGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAg                                                                                                    >  2:1465831/1‑140 (MQ=255)

                                     gAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAgtg                                                                                                  <  1:36292/140‑1 (MQ=255)

                                          aGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAAAGGAGAGAGAGAACTGCAGTGTAGcc                                                                                             >  1:421905/1‑140 (MQ=255)

                                               gTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAgt                                                                                                   <  1:1096395/129‑1 (MQ=255)

                                               gTTGCATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTTATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAgt                                                                                                   >  2:1096395/1‑129 (MQ=255)

                                                 tGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCt                                                                                      >  2:1157670/1‑140 (MQ=255)

                                                    aTTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTc                                                                                   <  1:807175/140‑1 (MQ=255)

                                                     tttAGCAACACGGTCAGCACGGGTCATAGAACCAGATTTTATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCg                                                                                  <  2:535218/140‑1 (MQ=255)

                                                     tttAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCg                                                                                  >  1:185629/1‑140 (MQ=255)

                                                       tAGCAACACGGTCAGTACGGCTAATCGAACCAGTTTTCATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGcc                                                                                <  1:397045/140‑1 (MQ=255)

                                                         gCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTGGCCAGAACGGTGGGAGTTAACTGCAGTGTAGCCAGCATCTTTCGCCAt                                                                              >  2:494232/1‑140 (MQ=255)

                                                         gCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGTTAACTGCAATGTACCCAGCATCTTTCGCCAt                                                                              >  2:570725/1‑140 (MQ=255)

                                                             cacGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCTGAACGGTGGGAGGTAAATGCAGTGTCGCCAGCATCTTTCGCCATCTTg                                                                          >  2:609477/1‑140 (MQ=255)

                                                             cacGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTg                                                                          >  2:771027/1‑140 (MQ=255)

                                                             cacGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGAGTCGCCAGCATCTTTCGCCATCTTg                                                                          >  1:535218/1‑140 (MQ=255)

                                                               cGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGAt                                                                        <  2:270130/140‑1 (MQ=255)

                                                                 gTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTg                                                                      <  2:185629/140‑1 (MQ=255)

                                                                 gTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTg                                                                      <  1:1101220/140‑1 (MQ=255)

                                                                  tCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGc                                                                     >  2:1097416/1‑140 (MQ=255)

                                                                  tCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGc                                                                     >  1:965840/1‑140 (MQ=255)

                                                                  tCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGc                                                                     >  1:1059139/1‑140 (MQ=255)

                                                                  tCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGc                                                                     >  2:1066184/1‑140 (MQ=255)

                                                                  tCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGAAAACTGCAATGTAGCCAGCATCTTTCGCCATCTTGATTGc                                                                     >  2:653846/1‑140 (MQ=255)

                                                                     gAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGc                                                                  <  1:813516/140‑1 (MQ=255)

                                                                           cTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGt                                                            >  1:59847/1‑140 (MQ=255)

                                                                           cTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGCAGCCAGCATCTGTCGCCATCTGGATTGCAGCCAGAGt                                                            >  1:433641/1‑140 (MQ=255)

                                                                            tCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCa                                                                <  1:1503306/135‑1 (MQ=255)

                                                                              aTAGAACCAGTTTTGATTTGGCATGCAGCAGCACCAACAGCCAGGTCACCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTc                                                         <  2:433641/140‑1 (MQ=255)

                                                                              aTAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTc                                                         <  1:1469482/140‑1 (MQ=255)

                                                                                 gAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCAACTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGt                                                      <  1:77782/140‑1 (MQ=255)

                                                                                    ccAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGATGGGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAATGTAGCCAACATATTTGGCCATCTTGATTGAAGCCAGAGTTTCGGTCa                                                    >  2:430176/1‑140 (MQ=255)

                                                                                    ccAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGGGTAGCCAGCATCCTTCTCCATCTTGATTGCAGCCAGAGTTTCGGTCag                                                   >  2:556294/1‑140 (MQ=255)

                                                                                     cAGTTTTGATCTGGCCTGCAGCAGTACCAACACCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCaga                                                  >  2:808518/1‑140 (MQ=255)

                                                                                       gTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTc                                                         >  1:1125375/1‑131 (MQ=255)

                                                                                       gTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTc                                                         <  2:1125375/131‑1 (MQ=255)

                                                                                        ttttGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTACGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGaa                                               >  1:663806/1‑140 (MQ=255)

                                                                                            gATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGa                                           <  1:1157670/140‑1 (MQ=255)

                                                                                            gATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGa                                           >  2:1452481/1‑140 (MQ=255)

                                                                                                 ggCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTgg                                      >  2:1099729/1‑140 (MQ=255)

                                                                                                  gCCTGCAGCAGTACCAACAGCCGGGTCAGCGAT‑GGTAGCCTCTTCAGTCTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGt                                     <  2:421905/140‑1 (MQ=255)

                                                                                                  gCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGt                                     >  1:1019722/1‑140 (MQ=255)

                                                                                                  gCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGt                                     >  1:293927/1‑140 (MQ=255)

                                                                                                         gcagTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAAttt                              <  1:1332899/140‑1 (MQ=255)

                                                                                                           agTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGa                            <  2:101455/140‑1 (MQ=255)

                                                                                                            gTACCAACAGCCAGGTCAGCGAT‑GGTAGCGTCTTCAGCTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGAt                           <  1:494232/140‑1 (MQ=255)

                                                                                                            gTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCTGAGTTTCAGTCTGAAAACCGATCTGGTTGAATTTGAt                           >  1:529923/1‑140 (MQ=255)

                                                                                                            gTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGAt                           <  2:965840/140‑1 (MQ=255)

                                                                                                            gTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGAt                           >  1:1076173/1‑140 (MQ=255)

                                                                                                            gTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGAt                           <  1:1099729/140‑1 (MQ=255)

                                                                                                            gTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGAt                           <  2:1019722/140‑1 (MQ=255)

                                                                                                            gTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGAt                           >  2:1191362/1‑140 (MQ=255)

                                                                                                            gTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGAt                           <  2:218646/140‑1 (MQ=255)

                                                                                                            gTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGAt                           >  2:1493227/1‑140 (MQ=255)

                                                                                                            gTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGAt                           >  2:215634/1‑140 (MQ=255)

                                                                                                            gTACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGCTTCGGTCAGAGAACCGATCTGGTTGAATTTGAt                           >  2:18183/1‑140 (MQ=255)

                                                                                                            gTACCAACAGCCAGGCCAGCGAC‑GGAAGCTTCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGAt                           <  1:440154/140‑1 (MQ=255)

                                                                                                             tACCAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATc                          >  1:186107/1‑140 (MQ=255)

                                                                                                               ccAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAg                        >  1:1371535/1‑140 (MQ=255)

                                                                                                                cAACAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAgg                       <  1:771027/140‑1 (MQ=255)

                                                                                                                 aaCAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGa                      <  2:186107/140‑1 (MQ=255)

                                                                                                                 aaCAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGa                      <  1:1287612/140‑1 (MQ=255)

                                                                                                                  aCAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGAt                     >  2:1272331/1‑140 (MQ=255)

                                                                                                                  aCAGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGAACTGGTTGAATTTGATCAGGAt                     >  1:1256441/1‑140 (MQ=255)

                                                                                                                    aGCCAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATgg                   >  1:1302940/1‑140 (MQ=255)

                                                                                                                    aGCCAGGTCAGCGAT‑GGTAGCACCTTCAGTTTCGCCAGATCGGTGAGACATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATgg                   <  2:511376/140‑1 (MQ=255)

                                                                                                                     gCCAGGTCAGCGAT‑GGTAGCATTTTCAGTTTCGCCCGAATGGTGAGAGTTAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGa                  <  2:529923/140‑1 (MQ=255)

                                                                                                                      ccAGGTCAGCGAT‑GGTTCCATCCTCAGTTTAGCTAAAACGGTGAGAGAAAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAg                 <  2:728622/140‑1 (MQ=255)

                                                                                                                      ccAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAg                 >  2:1350972/1‑140 (MQ=255)

                                                                                                                      ccAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAg                 <  2:1371535/140‑1 (MQ=255)

                                                                                                                       cAGGTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGt                >  1:29479/1‑140 (MQ=255)

                                                                                                                          gTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAg             >  1:375100/1‑140 (MQ=255)

                                                                                                                          gTCAGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGAGCTGCTTCAATTTGCTCAGGATGGAGTTAg             >  1:728622/1‑140 (MQ=255)

                                                                                                                             aGCGAT‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGa          <  2:1302940/140‑1 (MQ=255)

                                                                                                                                  t‑GGTAGCATCTTCAGTTTCGCCAGAACGGTGAGTGTTAATTGCAGTGTCGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCt     <  2:663806/140‑1 (MQ=255)

                                                                                                                                     gTAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTGGCGATACCttt   >  2:1268115/1‑140 (MQ=255)

                                                                                                                                      tAGCATCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCtttt  <  2:293927/140‑1 (MQ=255)

TTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAACCAGTTTTGATCTGGCCTGCAGCAGTACCAACAGCCAGGTCAGCGAT‑GGTAGCGTCTTCAGTTTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTT  >  NC_000913/2906674‑2906946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`