| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 425,420 | T→C | V137A (GTG→GCG) | queA → | tRNA preQ1(34) S‑adenosylmethionine ribosyltransferase‑isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 425,420 | 0 | T | C | 97.9% | 165.0 / NA | 48 | V137A (GTG→GCG) | queA | tRNA preQ1(34) S‑adenosylmethionine ribosyltransferase‑isomerase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (22/25); minor base A (1/0); total (23/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.79e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCATATTCGCGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGATGACGAAAGTATTAACGCAACAATGACCGCGCGCCACGGCGCACTGTTTGAAGTCGAATTTAATGATGAACGCTCGGTGCTGGATATTCTCAACAGCATCGGCCATATGCCGCTGCCGCCGTATATCGACCGTCCGGACGAAGACGCTGACCGCGAACTTTATCAAACCGTTTATAGCGAAAAACCGGGCGCGGTTGCAGCCCCGACCGCAGGT > NC_000913/425288‑425556 | cgcATATTCGCGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGATGACGAAAGTATTAACGCAACAATGACCGCGCGCCACGGCGCACTGTTTGAAGTCGAATTTAATGATGAACGCTCGGCGCTgg < 2:379372/138‑1 (MQ=255)cgcATATTCGCGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGATGACGAAAGTATTAACGCAACAATGACCGCGCGCCACGGCGCACTGTTTGAAGTCGAATTTAATGATGAACGCTCGGCGCTgg > 1:379372/1‑138 (MQ=255) atatTCGCGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGATGACGAAAGTATTAACGCAACAATGACCGCGCGCCACGGCGCACTGTTTGAAGTCGAATTTAATGATGAACGCTCGGCGCTGGATAtt < 1:180122/140‑1 (MQ=255) tCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGATGACGAAAGTATTAACGCAACAATGACCGCGCGCCACGGCGCACTGTTTGAAGTCGAATTTAATGATGAACGCTCGGCGCTGGATATTCTCAACAGCAt < 2:1240178/140‑1 (MQ=255) tCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGATGACGAAAGTATTAACGCAACAATGACCGCGCGCCACGGCGCACTGTTTGAAGTCGAATTTAATGATGAACGCTCGGCGCTGGATATTCTCAACAGCAt < 1:1335347/140‑1 (MQ=255) cGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGATGACGAAAGTATTGACGCAACAATGACCGCGCGCCACGGCGCACTGTTTGAGGTCGAATTGAATAATGAACGCTCGGAGCTGGATATTCTCAACAGCATc > 2:429735/1‑140 (MQ=255) cGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGATGACGAAAGTATTAACGCAACAATGACCGCGCGCCACGGCGCACTGTTTGAAGTCGAATTTAATGATGAACGCTCGGCGCTGGATATTCTCAACAg < 2:974767/136‑1 (MQ=255) cGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGATGACGAAAGTATTAACGCAACAATGACCGCGCGCCACGGCGCACTGTTTGAAGTCGAATTTAATGATGAACGCTCGGCGCTGGATATTCTCAACAg > 1:974767/1‑136 (MQ=255) aaaCCTGGCGCAGAACTGCTGCTGGGCGATGACGAAAGTATTAACGCAACAATGACCGCGCGCCACGGCGCACTGTTTGAAGTCGAATTTAATGATGAACGCTCGGCGCTGGATATTCTCAACAGCATCGGCCATAtgcc > 2:1492267/1‑140 (MQ=255) agacagcAGAACTGCTGTTGGGGGATGACGAAAGTCTTACCGCAACAATGACCGCGCGCCACGGCGCACTGTTTGAAGTCGAATTTAATGATGAACGCTCGGCGCTGGATATTCTCAACAGCATCGGCCATAtgccgctg < 2:573368/135‑1 (MQ=255) tGGCGCAGAACTGCTTCTGGGCCATGACGCAAGTATTCACGCAACAATGACCGCGCGCCACGGCGCACTGTTTGAAGTCGAATTTAATGATGAACGCTCGGCGCTGGATATTCTCAACAGCATCGGCCATAtgccgctgc < 1:429735/140‑1 (MQ=255) gAACTGCTGCTGGGCGATGACGAAAGTATTAACGCAACAATGACCGCGCGCCACGGCGCACTGTTTGAAGTCGAATTTAATGATGAACGCTCGGCGCTGGATATTCTCAACAGCATCGGCCATATGCCGCTGCCGCCGta < 2:4814/140‑1 (MQ=255) ctgctgGGCGATGACGAAAGTATTAACGCAACAATGACCGCGCGCCACGGCGCACTGTTTGAAGTCGAATTTAATGATGAACGCTCGGCGCTGGATATTCTCAACAGCATCGGCCATAtgccgctg < 2:1257119/126‑1 (MQ=255) ctgctgGGCGATGACGAAAGTATTAACGCAACAATGACCGCGCGCCACGGCGCACTGTTTGAAGTCGAATTTAATGATGAACGCTCGGCGCTGGATATTCTCAACAGCATCGGCCATAtgccgctg > 1:1257119/1‑126 (MQ=255) ctgctgGGCGATGACGAAAGTATTAACGCAACAATGACCGCGCGCCACGGCGCACTGTTTGAAGTCGAATTTAATGATGAACGCTCGGCGCTGGATATTCTCAACAGCATCGGCCATATGCCGCTGCCGCCGTATATCGa > 1:190857/1‑140 (MQ=255) ctgctgGGCGATAACGAAAGTATTAACGCAACAATGACCGCGCGCCACGGCGCACTGTTTGAAGTCGAATTTAATGATGAACGCTCGGCGCTGGATATTCTCAACAGCATCGGCCATATGCCGCTGCCGCCGTATATCGa > 2:1304618/1‑140 (MQ=255) ctgGGCGATGACGAAAGTATTAACGCAACAATGACCGCGCGCCACGGCGCACTGTTTGAAGTCGAATTTAATGATGAACGCTCGGCGCTGGATATTCTCAACGGCATCGGACATATGCCGCTGCCGCCGTATATctgtct > 1:573368/1‑135 (MQ=255) tgGGCGATGACGAAACTATTAATGCTACACTGACCGCCCGCCACGGCGCACTGTTTGAAGTCGAATTTAATGATGAACGCTCGGCGCTGGATATTCTCAACAGCATCGGCCATATGCCGCTGCCGCCGTATATCGGCCGt < 1:724758/140‑1 (MQ=255) gggCGATGACGAAAGTATTAACGCAACAATGACCGCGCGCCACGGCGCACTGTTTGAAGTCGAATTTAATGATGAACGCTCGGCGCTGGATATTCTCAACAGCATCGGCCATATGCCGCTGCCGCCGTATATCGACCGTc < 1:1074488/140‑1 (MQ=255) gCGATGACGAAAGTATTAACGCAACAATGACCGCGCGCCACGGCGCACTGTTTGAAGTCGAATTTAATGATGAACGCTCGGCGCTGGATATTCTCACCAGCATCGGCCATATGCCGCTGCCGCCGTATATCGACCGTCCg < 2:190857/140‑1 (MQ=255) tAACGCAACAATGACCGCGCGCCACGGCGCACTGTTTGAAGTCGAATTTAATGATGAACGCTCGGCGCTGGATATTCTCAACAGCATCGGCCATATGCCGCTGCCGCCGTATATGGATCGTCCGGACGAAGACGCTGAcc > 1:402376/1‑140 (MQ=255) tAACGCAACAATGACCGCGCGCCACGGCGCACTGTTTGAAGTCGAATTTAATGATGAACGCTCGGCGCTGGATATTCTCAACAGCATCGGCCATATGCCGCTGCCGCCGTATATCGACCGTCCGGACGAAGACGCTGAcc < 1:282133/140‑1 (MQ=255) tAACGCAACAATGACCGCGCGCCACGGCGCACTGTTTGAAGTCGAATTTAATGATGAACGCTCGGCGCTGGATATTCTCAACAGCATCGGCCATATGCCGCTGCCGCCGTATATCGACCGTCCGGACGAAGACGCTGAcc > 1:1075000/1‑140 (MQ=255) aaCGCAACAATGACCGCGCGCCACGGCGCACTGTTTGAAGTCGAATTTAATGATGAACGCTCGGCGCTGGATATTCTCAACAGCATCGGCCATATGCCGCTGCCGCCGTATATCGACCGTCCGGACGAAGACGCTGACcg < 2:1075000/140‑1 (MQ=255) aaCGCAACAATGACCGCCCGCCCCGGCGCACAGTTCGAATTCGAATTTCATGATGAACGCTCGGCGCTGGATATTCTCAACAGCATCGGCCATATGCCGCTGCCGCCGTATATCGACCGTCCGGACGAAGACGCTGACcg < 2:402376/140‑1 (MQ=255) caacaaTGACCGCGCGCCACGGCGCACTGTTTGAAGTCGAATTTAATGATGAACGCTCGGCGCTGGATATTCTCAACAGCATCGGCCATATGCCGCTGCCGCCGTATATCGACCGTCCGGACGAAGACGCTGACCGCGaa < 2:808324/140‑1 (MQ=255) agacaaTGACCACGCGCAACGGCGCACTGTTAGAATTCGCATTTAATGATGAACGCTCGGCGCTGGATATTCTCAACAGCATCGGCCATATGCCGCTGCCGCCGTATATCGACCGTCCGGACGAAGACGCTGACCGCGaa < 2:644807/138‑1 (MQ=255) acaaTGACCGCGCGCCACGGCGCACTGTTTGAAGTCGAATTTAATGATGAACGCTCGGCGCTGGATATTCTCAACAGCATCGGCCATATGCTGCTGCCGCCGTAAATCTCCCGTCCGGACGACAACGCTGACCGCGAACt > 2:723077/1‑140 (MQ=255) acaaTGACCGCGCGCCACGGCGCACTGTTTGAAGTCGAATTTAATGATGAACGCTCGGCGCTGGATATTCTCAACAGCATCGGCCATATGCCGCTGCCGCCGTATATCGACCGTCCGGACGAAGACGCTGACCGCGAACt > 2:930912/1‑140 (MQ=255) acaaTGACCGCGCGCCACGGCGCACTGTTTGAAGTCGAATTTAATGATGAACGCTCGGCGCTGGATATTCTCAACAGCATCGGCCATATGCCGCTGCCGCCGTATATCGACCGTCCGGACGAAGACGCTGACCGCGAACt > 2:1043109/1‑140 (MQ=255) gACCGGCCGCCACGGCGCACTGTTTGAAGTCGAATTTAATGATGAACGCTCGGCGCTGGATATTCTCAACAGCATCGGCCATATGCCGCTGCCGCCGTATATCGACCGTCCGGACGAAGACGCTGACCGCGAACTTTATc < 1:1057641/140‑1 (MQ=255) gACCGCGCGCCACGGCGCACTGTTTGAAGTCGAATTTAATGATGAACGCTCGGCGCTGGATATTCTCAACAGCATCGGCCATATGCCGCTGCCGCCGTATATCGACCGTCCGGACGAAGACGCTGACCGCGAACTTTATc < 1:930912/140‑1 (MQ=255) ccGCGCGCCACGGCGCACTGTTTGAAGTCGAATTTAATGATGAACGCTCGGCGCTGGATATTCTCAACAGCATCGGCCATATGCCGCTGCCGCCGTATATCGTCCGTCCGCACGAAGTCGCTGAACGCGAACTTTATCaa > 1:738992/1‑140 (MQ=255) gcgcCACGGCGCACTGTTTGAAGTCGAATTTAATGATGAACGCTCGGCGCTGGATATTCTCAACAGCATCGGCCATATGCCGCTGCCGCCGTATATCGACCGTCCGGACGAAGATGCCGACCGCTAACTTTAACAAAcct > 1:644807/1‑139 (MQ=255) cgcCACGGCGCACTGTTTGAAGTCGAATTTAATGATGAACGCTCGGCGCTGGATATTCTCAACAGCATCGGCCATATGCCGCTGCCGCCGTATATCGACCGTCCGGACGAAGACGCTGACCGCGAACTTTATc > 2:376815/1‑133 (MQ=255) cgcCACGGCGCACTGTTTGAAGTCGAATTTAATGATGAACGCTCGGCGCTGGATATTCTCAACAGCATCGGCCATATGCCGCTGCCGCCGTATATCGACCGTCCGGACGAAGACGCTGACCGCGAACTTTATc < 1:376815/133‑1 (MQ=255) tGTTTGAAGTCGAATTTAATGATGAACGCTCGGCGCTGGATATTCTCAACAGCATCGGCCATATGCCGCTGCCGCCGTATATCGACCGTCCGGACGAAGACGCTGACCGCGAACTTTATCAAACCGTTTATAGCGaaaaa > 1:1292884/1‑140 (MQ=255) tttGAAGTCGAATTTAATGATGAACGCTCGGCGCTGGATATTCTCAACAGCATCGGCCATATGCCGCTGCCGCCGTATATCGACCGTCCGGACGAAGACGCTGACCGCGAACTTTATCAAACCGTTTATAGCGAAAAAcc > 2:1478565/1‑140 (MQ=255) gTCGAATTTAATGATGAACGCTCGGCGCTGGATATTCTCAACAGCATCGGCCATATGCCGCTGCCGCCGTATATCGACCGTCCGGACGAAGACGCTGACCGCGAACTTTATCAAACCGTTTATAGCGAAAAACCGGgcgc < 1:1043109/140‑1 (MQ=255) gTCGAATTTAATGATGAACGCTCGGCGCTGGATATTCTCAACAGCATCGGCCATATGCCGCTGCCGCCGTATATCGACCGTCCGGACGAAGACGCTGACCGCGAACTTTATCAAACCGTTTATAGCGAAAAACCGGgcgc < 1:1350999/140‑1 (MQ=255) gTCGAATTTAATGATGAACGCTCGGCGCTGGATATTCTCAACAGCATCGGCCATATGCCGCTGCCGCCGTATATCGACCGTCCGGACGAAGACGCTGACCGCGAACTTTATCAAACCGTTTATAGCGAAAAACCGGgcgc < 1:1492267/140‑1 (MQ=255) cGAATTTAATGATGAACGCTCGGCGCTGGATATTCTCAACAGCATCGGCCATATGCCGCTGCCGCCGTATATCGACCGTCCGGACGAAGACGCTGACCGCGAACTTTATCAAACCGTTTATAGCGAAAAACCGGGCGCgg > 1:773193/1‑140 (MQ=255) cGAATTTAATGATGAACGCTCGGCGCTGGATATTCTCAACAGCATCGGCCATATGCCGCTGCCGCCGTATATCGACCGTCCGGACGAAGACGCTGACCGCGAACTTTATCAAACCGTTTATAGCGAAAAACCGGGCGCgg > 1:1111192/1‑140 (MQ=255) aaTTTAATGATGAACGCTCGGCGCTGGATATTCTCAACAGCATCGGCCATATGCCGCTGCCGCCGTATATCGACCGTCCGGACGAAGACGCTGACCGCGAGCTTTATCCAACCGTTTATAGCTAAAAACCGGGCGCGGtt > 2:534382/1‑140 (MQ=255) aaTTTAATGATGAACGCTCGGCGCTGGATATTCTCAACAGCATCGGCCATATGCCGCTGCCGCCGTATATCGACCGTCCGGACGAAGACGCTGACCGCGAACTTTATCAAACCGTTTATAGCGAAAAACCGGGCGCGGtt > 1:947158/1‑140 (MQ=255) aaTTTAATGATGAACGCTCGGCGCTGGATATTCTCAACAGCATCGGCCATATGCCGCTGCCGCCGTATATCGACCGTCCGGACGAAGACGCTGACCGCGAACATTATCAAGCCGTTTGTAGCGAAAAACCGGGCGCGGtt > 1:488305/1‑140 (MQ=255) gatgaACGCTCGGCGCTGGATATTCTCAACAGCATCGCCCATATGCCGCTGCCGCCGTATATCGACCGTCCGGACGAAGACGCTGACCGCGAACTTTATCAAACCGTTTATAGCGAAAAACCGGGCGCGGTTGCAGcccc < 1:684024/140‑1 (MQ=255) aCGCTCGGCGCTGGATATTCTCAACAGCATCGGCCATATGCCGCTGCCGCCGTATATCGACCGTCCGGACGAAGACGCTGACCGCGAACTTTATCAAACCGTTTATAGCGAAAAACCGGGCGCGGTTGCAGCCCCGACCg < 2:773193/140‑1 (MQ=255) cGGCGCTGGATATTCTCAACAGCATCGGCCATATGCCGCTGCCGCCGTATATCGACCGTCCGGACGAAGACGCTGACCGCGAACTTTATCAAACCGTTTATAGCGAAAAACCGGGCGCGGTTGCAGCCCCGACCGCAGGt < 1:840627/140‑1 (MQ=255) | CGCATATTCGCGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGATGACGAAAGTATTAACGCAACAATGACCGCGCGCCACGGCGCACTGTTTGAAGTCGAATTTAATGATGAACGCTCGGTGCTGGATATTCTCAACAGCATCGGCCATATGCCGCTGCCGCCGTATATCGACCGTCCGGACGAAGACGCTGACCGCGAACTTTATCAAACCGTTTATAGCGAAAAACCGGGCGCGGTTGCAGCCCCGACCGCAGGT > NC_000913/425288‑425556 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |