Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,957,264	A→G	Y541H (TAT→CAT)	ptrA ←	protease 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,957,264	0	A	G	98.9%	312.9 / NA	90	Y541H (TAT→CAT)	ptrA	protease 3
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (37/52);  minor base C (0/1);  total (37/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAG  >  NC_000913/2957130‑2957389                                                                                                                                       |                                                                                                                              gTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCGGGCGGACGTCAGCTTTGGGCTCGCTGGCAAAATGACGGc                                                                                                                          >  2:599604/1‑140 (MQ=255) gTTGCTTAACTGATCAACCGCCAGCCCTGCGAGATTATCCTTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGc                                                                                                                          <  2:605614/140‑1 (MQ=255)     cTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTgg                                                                                                                      <  2:905822/140‑1 (MQ=255)           tGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCACGCTGACATCAGCTTTGGGCTCGCTGGCAAATTGACGGCTTGGCGCATa                                                                                                                >  2:580358/1‑140 (MQ=255)            gATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATac                                                                                                               >  2:782048/1‑140 (MQ=255)            gATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATac                                                                                                               >  1:178896/1‑140 (MQ=255)             aTCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGc                                                                                                                          <  2:1536479/128‑1 (MQ=255)             aTCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGc                                                                                                                          >  1:1536479/1‑128 (MQ=255)               cAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATAcacc                                                                                                            >  1:784740/1‑140 (MQ=255)                   cgcCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGGCGGCTTGGCGCATACACCAcgc                                                                                                        >  1:517724/1‑140 (MQ=255)                       aGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAg                                                                                                    >  2:368053/1‑140 (MQ=255)                       aGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAg                                                                                                    >  1:147653/1‑140 (MQ=255)                             gCGAGATAATCATTGTGCGCAACCAACACCTGATTGCCGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGa                                                                                              <  1:396979/140‑1 (MQ=255)                             gCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGa                                                                                              >  1:766002/1‑140 (MQ=255)                             gCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGa                                                                                              <  2:845797/140‑1 (MQ=255)                             gCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTAGCGCATACACCACGCGCAGATTCGa                                                                                              >  2:1503849/1‑140 (MQ=255)                              cGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGGTTGCCGTATACACGACGCGCAGATTCGAc                                                                                             >  1:469819/1‑140 (MQ=255)                              cGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGATCGCTGGCTAAAGGACGGCTTGGCGCATACACCACGCGCAGATTCGAc                                                                                             >  2:471521/1‑140 (MQ=255)                               gagaTAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACt                                                                                            <  1:953942/140‑1 (MQ=255)                                        aTTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATcaacaa                                                                                   >  2:287995/1‑140 (MQ=255)                                        aTTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATcaacaa                                                                                   >  1:384935/1‑140 (MQ=255)                                         ttGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAAt                                                                                  <  2:1117083/140‑1 (MQ=255)                                         ttGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAAt                                                                                  <  1:782048/140‑1 (MQ=255)                                         ttGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAAt                                                                                  <  1:368053/140‑1 (MQ=255)                                         ttGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAAt                                                                                  <  1:1416453/140‑1 (MQ=255)                                         ttGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAAt                                                                                  <  1:1064884/140‑1 (MQ=255)                                           gAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCa                                                                                <  1:1034146/140‑1 (MQ=255)                                              cgcAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGct                                                                             <  2:147653/140‑1 (MQ=255)                                              cgcAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGct                                                                             >  2:761585/1‑140 (MQ=255)                                              cgcAAACATCACCTGATCGCGGCCGCTGTCCCTGGTTTTGGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGct                                                                             <  2:517724/140‑1 (MQ=255)                                                   aCATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGa                                                                        <  2:178896/140‑1 (MQ=255)                                                    cATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGAt                                                                       <  2:191251/140‑1 (MQ=255)                                                    cATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGAt                                                                       <  2:50955/140‑1 (MQ=255)                                                    cATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGAt                                                                       <  2:766002/140‑1 (MQ=255)                                                     aTCACCTGATTGCGGGCGCTGTCCAAGGCCTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATg                                                                      <  1:599604/140‑1 (MQ=255)                                                        aCCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTc                                                                   >  2:1309318/1‑140 (MQ=255)                                                        aCCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTc                                                                   >  2:1170035/1‑140 (MQ=255)                                                         ccTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCg                                                                  <  1:1153031/140‑1 (MQ=255)                                                         ccTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCg                                                                  <  2:384935/140‑1 (MQ=255)                                                            gATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTAt                                                               >  1:1242215/1‑140 (MQ=255)                                                              ttGCGGGCGCTGTCCATTGCTTACGGATTACTCAATATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTAttt                                                             <  2:469819/140‑1 (MQ=255)                                                               tGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGACTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATttc                                                            >  2:987375/1‑140 (MQ=255)                                                                   ggCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTtcttct                                                        <  1:7992/140‑1 (MQ=255)                                                                   ggCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTtcttct                                                        >  2:1220766/1‑140 (MQ=255)                                                                       cTGTCCATGGCTTTCTGATTACACACAATTAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGa                                                    <  1:471521/140‑1 (MQ=255)                                                                         gTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACt                                                  >  1:1458327/1‑140 (MQ=255)                                                                            cATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTaa                                               <  1:1220766/140‑1 (MQ=255)                                                                             aTGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAAt                                              <  1:176620/140‑1 (MQ=255)                                                                                gCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAg                                           >  1:1295687/1‑140 (MQ=255)                                                                                  tttCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATAGTCATAATTCTTCGCTGACTTAATCAGCg                                         >  1:528155/1‑140 (MQ=255)                                                                                    tCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCGACAATCAGGTCTGGATAGTCGTATTGCTTCACTGACTTAATCAGCgag                                       >  2:407114/1‑140 (MQ=255)                                                                                    tCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTGTGGATGGGCGTATTTCTTCTCTGACTTAATCAGCgag                                       >  1:526688/1‑140 (MQ=255)                                                                                    tCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCgag                                       >  1:293864/1‑140 (MQ=255)                                                                                    tCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCgag                                       <  1:287995/140‑1 (MQ=255)                                                                                    tCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCgag                                       >  1:771121/1‑140 (MQ=255)                                                                                    tCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCgag                                       >  1:1409042/1‑140 (MQ=255)                                                                                      ggATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGaa                                     <  2:1213463/140‑1 (MQ=255)                                                                                       gATTACGCAAAATCATGCTGACTTCTGCTCTGGGCTCTCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGaaa                                    <  1:407114/140‑1 (MQ=255)                                                                                          tACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAatca                                 <  2:293864/140‑1 (MQ=255)                                                                                          tACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAatca                                 >  2:275496/1‑140 (MQ=255)                                                                                           aCGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAatcat                                <  2:784740/140‑1 (MQ=255)                                                                                           aCGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAatcat                                <  1:761585/140‑1 (MQ=255)                                                                                           aCGCAAAATCAGGCTGACATCAGCTTTGGGCGCGCCGGCTAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAatcat                                <  2:526688/140‑1 (MQ=255)                                                                                            cGCAAAATCAGGCTGACCTCAGCTTTGGGCTCGCTCGCATAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAatcatc                               <  2:528155/140‑1 (MQ=255)                                                                                              cAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAg                             <  2:1029790/140‑1 (MQ=255)                                                                                              cAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCCTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAg                             <  2:1262086/140‑1 (MQ=255)                                                                                              cAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACCGCTTGGCGCATACACCACGCGCAGATTCGACTCATCGACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAg                             <  2:223382/140‑1 (MQ=255)                                                                                              cAAAATCAGGCTGACATCAGCTATGTGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAg                             <  1:634462/140‑1 (MQ=255)                                                                                               aaaaTCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAgg                            <  2:946306/140‑1 (MQ=255)                                                                                                    cAGGCTGACATCAGCTTTGGGCTCGCTGGCACAATCACGCCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAatat                       <  2:470226/140‑1 (MQ=255)                                                                                                    cAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATTAGCGAGAAATCATCAGGAatat                       <  2:771121/140‑1 (MQ=255)                                                                                                    cAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAatat                       <  1:1503849/140‑1 (MQ=255)                                                                                                    cAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAatat                       <  1:1410024/140‑1 (MQ=255)                                                                                                    cAGGCTGACATCAGCCTTGGGCTCGCTGGTAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAatat                       <  1:511750/140‑1 (MQ=255)                                                                                                    cAGACTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAatat                       <  1:987375/140‑1 (MQ=255)                                                                                                      ggCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATaa                     >  1:121705/1‑140 (MQ=255)                                                                                                      ggCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGCTCGTATTTCTTCACTGTCTTAATCAGCGAGGAATCATCAGGAATATaa                     >  2:645395/1‑140 (MQ=255)                                                                                                       gCTGACATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAg                    >  2:821438/1‑140 (MQ=255)                                                                                                           aCATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTCTTTCTTCTTTGAGTTACTCAGCGAGAAATCTTCAGGAATATAAGGGtt                >  2:486529/1‑140 (MQ=255)                                                                                                            cATCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTa               <  2:1242215/140‑1 (MQ=255)                                                                                                              tCAGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAg             <  2:1494509/140‑1 (MQ=255)                                                                                                                aGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGct           <  2:836156/140‑1 (MQ=255)                                                                                                                aGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGct           <  2:1242370/140‑1 (MQ=255)                                                                                                                aGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGct           <  2:1295687/140‑1 (MQ=255)                                                                                                                aGCTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGct           <  2:1409042/140‑1 (MQ=255)                                                                                                                  cTTTGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGctct         >  1:11917/1‑140 (MQ=255)                                                                                                                     tGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGc      <  1:821438/140‑1 (MQ=255)                                                                                                                     tGGGCTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAAGCATGAGGAATATATGGGTTAAGCTCTGGc      >  1:447004/1‑140 (MQ=255)                                                                                                                         cTCGCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAag  <  1:275496/140‑1 (MQ=255)                                                                                                                            gCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAag  >  2:354450/1‑137 (MQ=255)                                                                                                                            gCTGGCAAAATGACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAag  <  1:354450/137‑1 (MQ=255)                                                                                                                                       |                                                                                                                              GTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGGCAAAG  >  NC_000913/2957130‑2957389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑