BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,964,480	G→A	P226S (CCG→TCG)	thyA ←	thymidylate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,964,480	0	G	A	98.0%	325.2 / ‑2.5	102	P226S (CCG→TCG)	thyA	thymidylate synthase
Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (51/49); total (52/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01

Only 100 of 103 total aligned reads displayed.

TGGCAGGATGTTTCGTAATTAGATAGCCACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGGACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTGCTGTACAGATGCGTGTCGCCACCGGTCCAGACAAAATCACCCACTTCCAGATCGCACTGCTGCGCCATCATATGCACCAATAACG  >  NC_000913/2964343‑2964611

tGGCAGGATGTTTCGTAATTAGATAGCCACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGGCGAAGATGAATTAGGGTTTACGTTTGATAATCAACTTCGGCAGCGAAc                                                                                                                                   >  2:587504/1‑140 (MQ=255)

       aTGTTTCGTAATTAGATAGCCACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTc                                                                                                                            >  2:1112868/1‑140 (MQ=255)

        tGTTTCGTAATTAGATAGCCACGGGCGCTTTAATGCCCGAATGCGGATGGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTcg                                                                                                                           <  2:586437/140‑1 (MQ=255)

          tttCGTAATTAGATAGCCACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTcgcg                                                                                                                         >  1:1515856/1‑140 (MQ=255)

          tttCGTAATTAGATAGCCACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTcgcg                                                                                                                         <  1:36844/140‑1 (MQ=255)

            tCGTAATTAGATAGCCACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGc                                                                                                                       >  1:292065/1‑140 (MQ=255)

            tCGTAATTAGATAGCCACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGc                                                                                                                       <  2:292065/140‑1 (MQ=255)

            tCGTAATTAGATAGCCACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAACCAATTTGGGCAGCCAACGCGGTTCGCGGc                                                                                                                       >  1:576450/1‑140 (MQ=255)

               tAATTAGATAGCCACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTa                                                                                                                    >  2:1152344/1‑140 (MQ=255)

                 aTTAGATAGCCACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTcg                                                                                                                           <  1:1253302/131‑1 (MQ=255)

                 aTTAGATAGCCACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTcg                                                                                                                           >  2:1253302/1‑131 (MQ=255)

                  ttAGATAGCCACCGGCGATTTAATGCCCGGATGCGGATCTTAGCCTTCTATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAAtt                                                                                                                 <  2:576450/140‑1 (MQ=255)

                    aGATAGCCACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAAGCAACTTCGGCATCGTACGCGGATCGCGGCTTAATTGc                                                                                                               >  1:720885/1‑140 (MQ=255)

                       tAGCCACCGGCGCTTTAGTGCCCGGATGCGGTTCGTAGCTTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGa                                                                                                            <  2:662849/140‑1 (MQ=255)

                         gCCACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCtt                                                                                                                     >  2:43259/1‑129 (MQ=255)

                         gCCACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCtt                                                                                                                     <  1:43259/129‑1 (MQ=255)

                          ccACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGa                                                                                                         >  2:1006293/1‑140 (MQ=255)

                          ccACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGa                                                                                                         >  2:874816/1‑140 (MQ=255)

                          ccACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTGCGGCAGCGAACTCGGTTCGCGTCTTAATTGCAGATGa                                                                                                         >  1:410429/1‑140 (MQ=255)

                              cGGCGCTTTAATGCCCGGATGCGGATCGTGGCCTTCATTCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGttt                                                                                                     <  1:587504/140‑1 (MQ=255)

                               ggCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCCGCGAACGAGGTACGAGGCTTACTTGCAGATGAGTTTg                                                                                                    >  1:722896/1‑140 (MQ=255)

                               ggCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTg                                                                                                    >  2:1181129/1‑140 (MQ=255)

                               ggCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTg                                                                                                    >  1:993945/1‑140 (MQ=255)

                               ggCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTg                                                                                                    <  1:1181129/140‑1 (MQ=255)

                                gcgcTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTg                                                                                                                >  1:1356453/1‑127 (MQ=255)

                                gcgcTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTg                                                                                                                <  2:1356453/127‑1 (MQ=255)

                                  gcTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATc                                                                                                 <  2:1443064/140‑1 (MQ=255)

                                    tttAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGtt                                                                                                      >  1:952620/1‑133 (MQ=255)

                                    tttAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGtt                                                                                                      <  2:952620/133‑1 (MQ=255)

                                       aaTGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCatat                                                                                            <  1:1152344/140‑1 (MQ=255)

                                          gCCCGGATGCGGATCGTAGCCTTCAATCTCACAGTCTTCGAAACGGTAATCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGt                                                                                         <  2:548443/140‑1 (MQ=255)

                                          gCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGt                                                                                         <  2:1038921/140‑1 (MQ=255)

                                          gCCCGGATGCGGATCGTAGCCTCCAATCTCATAGTTTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGt                                                                                         <  2:653287/140‑1 (MQ=255)

                                            ccGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGATGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTg                                                                                       <  1:121226/140‑1 (MQ=255)

                                            ccGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTg                                                                                       <  2:1127002/140‑1 (MQ=255)

                                            ccGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTg                                                                                       <  2:1515856/140‑1 (MQ=255)

                                             cGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTGc                                                                                      >  2:1011309/1‑140 (MQ=255)

                                                 tGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTGCTGTa                                                                                  <  1:1384548/140‑1 (MQ=255)

                                                      aTCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGt                                                                                         >  2:1343863/1‑128 (MQ=255)

                                                      aTCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGt                                                                                         <  1:1343863/128‑1 (MQ=255)

                                                       tCGTAGCCTTCAATCTTCAAGTCTTCGAAACGGGACTCGCAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTGCTGTACAGATg                                                                            <  2:754594/140‑1 (MQ=255)

                                                          tAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCCGATGAGTTTGAGCCATATGGTGGCTGTACAGATGCgt                                                                         >  1:754594/1‑140 (MQ=255)

                                                          tAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATgg                                                                                          >  1:905327/1‑123 (MQ=255)

                                                          tAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATgg                                                                                          <  2:905327/123‑1 (MQ=255)

                                                          tAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTGCTGTACAGATg                                                                            >  1:863040/1‑137 (MQ=255)

                                                          tAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTGCTGTACAGATg                                                                            <  2:863040/137‑1 (MQ=255)

                                                          tAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTGCTGTACAGATGCgt                                                                         >  1:1151759/1‑140 (MQ=255)

                                                          tAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCAGCTTAATTGCAGTTGACTTTGATCCATATGATTGCTGTACAGATGCgt                                                                         >  2:493946/1‑140 (MQ=255)

                                                             ccTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTGCTGTACAGATGCGTGTc                                                                      >  1:247711/1‑140 (MQ=255)

                                                              cTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGACTGTCATCCATATGGTTGATGTACAGATGctgtctc                                                                     >  2:673091/1‑134 (MQ=255)

                                                                tCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTGCTGTACAGATGCGTGTCGcc                                                                   >  1:88200/1‑140 (MQ=255)

                                                                  aaTCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTGCTGTACAGATGCGTGTCGCCAc                                                                 >  2:837815/1‑140 (MQ=255)

                                                                  aaTCTCAAAGTCTTCGAAACGGTAGTCGAAGATGAATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTGCTGTACAGATGCGTGTCGCCAc                                                                 >  2:771158/1‑140 (MQ=255)

                                                                   aTCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTGCTGTACAGATGCGTGTCGCCAcc                                                                >  2:547171/1‑140 (MQ=255)

                                                                       cAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATTTGATTGCTGTGCAGATGCGTGTCGACATCGGTc                                                            >  2:737664/1‑140 (MQ=255)

                                                                         aaGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTGCTGTACAGATGCGTGTCGCCACCGGTCCa                                                          >  2:119336/1‑140 (MQ=255)

                                                                             cTTCGAAACGGTAGTTGAAGATGGATTCGCGTTTACGCTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTGCTGTACAGATGCGTGTCGCCATCGGTCCAGACa                                                      <  1:737664/140‑1 (MQ=255)

                                                                              ttCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGGACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTGCTGTACAGATGCGTGTCGCCACCGGTcc                                                           <  2:1182475/134‑1 (MQ=255)

                                                                              ttCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGGACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTGCTGTACAGATGCGTGTCGCCACCGGTcc                                                           >  1:1182475/1‑134 (MQ=255)

                                                                              ttCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTGCTGTACAGATGCGTGTc                                                                      >  1:291612/1‑123 (MQ=255)

                                                                              ttCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTGCTGTACAGATGCGTGTCGCCACCGGt                                                             <  1:1361640/132‑1 (MQ=255)

                                                                              ttCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTGCTGTACAGATGCGTGTCGCCACCGGt                                                             >  2:1361640/1‑132 (MQ=255)

                                                                              ttCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTGCTGTACAGATGCGTGTCGCCACCGGTCCAGACaa                                                     >  1:1032478/1‑140 (MQ=255)

                                                                              ttCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTGCTGTACAGATGCGTGTCGCCACCGGTCCAGACaa                                                     >  2:193732/1‑140 (MQ=255)

                                                                              ttCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGCTTGCTGTACAGATGAGTGTCGCCACCGGTCCAGACaa                                                     >  1:611317/1‑140 (MQ=255)

                                                                               tCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTGCTGGACAGATGCGTGTCGCCACCGGTCCAGACaaa                                                    >  2:551137/1‑140 (MQ=255)

                                                                                cGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTGCTGTACAGATGCGTGTCGCCACCGGTCCAGACaaaa                                                   >  2:376615/1‑140 (MQ=255)

                                                                                     cGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTGCTGTACAGATGCGTGTCGCCACCGGTCCAGACAAAATCAcc                                              <  1:1047871/140‑1 (MQ=255)

                                                                                     cGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTGCTGTACAGATGCGTGTCGCCACCGGTCCAGACAAAATCAcc                                              <  1:547171/140‑1 (MQ=255)

                                                                                     cGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTGCTGTACAGATGCGTGTCGCCACCGGTCCAGACAAAATCAcc                                              <  2:88200/140‑1 (MQ=255)

                                                                                     cGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTGCTGTACAGATGCGTGTCGCCACCGGTCCAGACAAAATCAcc                                              <  1:1015647/140‑1 (MQ=255)

                                                                                     cGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTGCTGTACAGATGCGTGTCGCCACCGGTCCAGACAAAATCAcc                                              <  2:1032478/140‑1 (MQ=255)

                                                                                     cGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTGCTGTACAGATGCGTGTCGCCACCGGTCCAGACAAAATCAcc                                              <  1:193732/140‑1 (MQ=255)

                                                                                     cGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTGCTGTACAGATGCGTGTCGCCACCGGTCCAGACAAAATCAcc                                              <  1:119336/140‑1 (MQ=255)

                                                                                     cGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTGCTGTACAGATGCGTGTCGCCACCGGTCCAGACAAAATCAcc                                              <  2:247711/140‑1 (MQ=255)

                                                                                      ggTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTGCTGTACAGATGCGTGTCGCCACCGGTCCAGACAAAATCAccc                                             >  1:930953/1‑140 (MQ=255)

                                                                                      ggTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTGCTGTACAGATGCGTGTCGCCACCGGTCCAGACAAAATCAcc                                              <  2:369948/139‑1 (MQ=255)

                                                                                      ggTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTGCTGTACAGATGCGTGTCGCCACCGGTCCAGACAAAATCAcc                                              >  1:369948/1‑139 (MQ=255)

                                                                                           tCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTGCTGTACAGATGCGTGTCGCCACCGGTCCAGACAAAATCACCCACTTc                                        >  2:1362319/1‑140 (MQ=255)

                                                                                                gATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTGCTGTACAGATGCGTGTCGCCACCGGTCCAGACAAAATCACCCACTTCCAGAt                                   >  2:364848/1‑140 (MQ=255)

                                                                                                 aTGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTGCCGTACAGATGCGTGTCGCCACCGGTCCAGACAAAATCACCCACTTCCAGATc                                  >  1:528688/1‑140 (MQ=255)

                                                                                                  tGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTGCTGTACAGATGCGTGTCGCCACCGGTCCAGACaaaa                                                   >  1:377591/1‑122 (MQ=255)

                                                                                                  tGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTGCTGTACAGATGCGTGTCGCCACCGGTCCAGACaaaa                                                   <  2:377591/122‑1 (MQ=255)

                                                                                                    gATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTGCTGTACAGATGCGTGTCGCCACCGGTCCAGACAAAATCACCCACTTCCAGATCGCa                               >  1:217607/1‑140 (MQ=255)

                                                                                                       tCGGGTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTGCTGTACAGATGCGTGTCGCCACCGGTCCAGACAAAATCACCCACTTCCAGATCGCActg                            <  1:1112868/140‑1 (MQ=255)

                                                                                                         gggTTTACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTGCTGTACAGATGCGTGTCGCCACCGGTCCAGACAAAATCACCCACTTCCAGATCGCActgct                          <  1:1362319/140‑1 (MQ=255)

                                                                                                              tACGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTGCTGTACAGATGCGTGTCGCCACCGGTCCAGACAAAATCACCCACTTCCAGATCGCACTGCTGCGcc                     >  2:851077/1‑140 (MQ=255)

                                                                                                               aCGTTTGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTGCTGTACAGATGCGTGTCGCCACCGGTCCAGACAAAATCACCCACTTCCAGATCGCACTGCTGCGCca                    <  2:239959/140‑1 (MQ=255)

                                                                                                                    tGATAATCAACTTCGGCCGCGATCGCGGTTCTCGGTTTATTTGCAGATTAGTTTGATCCATATGGTTGCTGTACAGATGCGTGTCGCCACCGGTCCAGACAAAATCACCCACTTCCAGATCGCACTGCTGCGCCATCata               <  2:477824/140‑1 (MQ=255)

                                                                                                                    tGATAATCAACTTCGGCCGCGAGCGCGGTTCTCGGGTTACTTGCAGATGAGTTTGATCCATATGGTTGCTGTACAGATGCGTGTCGCCACCGGTCCAGACAAAATCACCCACTTCCAGATCGCACTGCTGCGCCATCata               <  2:627266/140‑1 (MQ=255)

                                                                                                                    tGATAATCAACTTCGGCAGCGATCGCGGTTCGCGGCTTATTTGCAGATTAGTTTGATCCATATGGTTGCTGTACAGATGCGTGTCGCCACCGGTCCAGACAAAATCACCCACTTCCAGATCGCACTGCTGCGCCATCata               <  2:648292/140‑1 (MQ=255)

                                                                                                                    tGATAATCAACTTCGGCAGCGACCGCGGTTCCCGGATTAATTGCAGATAAGTTTGATCCATATGGTTGCTGTACAGATGCGTGTCGCCACCGGTCCAGACAAAATCACCCACTTCCAGATCGCACTGCTGCGCCATCata               <  2:410429/140‑1 (MQ=255)

                                                                                                                    tGATAATCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTGCTGTACAGATGCGTGTCGCCACCGGTCCAGACAAAATCACCCACTTCCAGATCGCACTGCTGCGCCATCata               <  1:771158/140‑1 (MQ=255)

                                                                                                                      aTAATCAATTTCGGCAGCGAACACGGTTCGTAGTTGAATCGCAGATGACTTTGATCCATATGGTTGCTGTACAGATGCGTGTCGCCACCGGTCCAGACAAAATCACCCACTTCCAGATCGCACTGCTGCGCCATCATATg             <  2:722896/140‑1 (MQ=255)

                                                                                                                      aTAATCAACTTCGGCAGCGAACCCGGTTCGCTGCTTAATCGCAGATGAATTTGATCCATATGGTTGCTGTACAGATGCGTGTCGCCACCGGTCCAGACAAAATCACCCACTTCCAGATCGCACTGCTGCGCCATCATATg             <  2:611317/140‑1 (MQ=255)

                                                                                                                          tCAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTGCTGTACAGATGCGTGTCGCCACCGGTCCAGACAAAATCACCCACTTCCCGATGGCCCTGCTGGGCCATCATATGCAcc         >  1:566531/1‑140 (MQ=255)

                                                                                                                           cAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTGCTGTACAGATGCGTGTCGCCACCGGTCCAGACAAAATCACCCACTTCCAGATCGCACTGCTGCGCCATCATATGCACCa        >  2:24286/1‑140 (MQ=255)

                                                                                                                           cAACTTCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTGCTGTACAGATGCGTGTCGCCACCGGTCCAGACAAAATCACCCACTTCCAAATCGCACTGCTGCCCCATCATATGCACCa        >  1:627266/1‑140 (MQ=255)

                                                                                                                                tCGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTGCTGTACAGATGCGTGTCGCCACCGGTCCAGACAAAGTCACCCACCTCCAGATAGCACTGCTGCGCCCTCATATGCACCAATAAc   >  2:559568/1‑140 (MQ=255)

                                                                                                                                 cGGCAGCGAACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTGCTGTACAGATGCGTGTCGCCACCGGTCCAGACAAAATCACCCACTTCCAGATCGCCCTGCTGAGCCATCGTATGCACCAATAAcg  >  1:739451/1‑140 (MQ=255)

TGGCAGGATGTTTCGTAATTAGATAGCCACCGGCGCTTTAATGCCCGGATGCGGATCGTAGCCTTCAATCTCAAAGTCTTCGAAACGGTAGTCGAAGATGGATTCGGGTTTACGTTTGATAATCAACTTCGGCAGCGGACGCGGTTCGCGGCTTAATTGCAGATGAGTTTGATCCATATGGTTGCTGTACAGATGCGTGTCGCCACCGGTCCAGACAAAATCACCCACTTCCAGATCGCACTGCTGCGCCATCATATGCACCAATAACG  >  NC_000913/2964343‑2964611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`