BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,990,286	T→C	K25K (AAA→AAG)	yqeK ←	protein YqeK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,990,286	0	T	C	98.8%	604.4 / ‑3.7	171	K25K (AAA→AAG)	yqeK	protein YqeK
Reads supporting (aligned to +/- strand): ref base T (2/0); new base C (75/94); total (77/94)
Fisher's exact test for biased strand distribution p-value = 2.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 175 total aligned reads displayed.

TAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCAC  >  NC_000913/2990149‑2990410

taACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGTATTTTTTTAACGGAATCCTTGGctt                                                                                                                            >  1:689271/1‑140 (MQ=255)

taACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGctt                                                                                                                            >  2:1028636/1‑140 (MQ=255)

 aaCTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTc                                                                                                                           >  1:392066/1‑140 (MQ=255)

 aaCTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTc                                                                                                                           >  2:1533460/1‑140 (MQ=255)

      tttttAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCttttt                                                                                                                      <  2:1138093/140‑1 (MQ=255)

       ttttAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTc                                                                                                                     >  1:18189/1‑140 (MQ=255)

       ttttAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTc                                                                                                                     >  2:825526/1‑140 (MQ=255)

       ttttAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTc                                                                                                                     >  2:1260439/1‑140 (MQ=255)

        tttAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCg                                                                                                                    >  1:1331948/1‑140 (MQ=255)

        tttAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCg                                                                                                                    >  2:977482/1‑140 (MQ=255)

             cTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTcc                                                                                                               <  1:1028636/140‑1 (MQ=255)

                tACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGaa                                                                                                            >  2:1258128/1‑140 (MQ=255)

                    ctTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTa                                                                                                        <  2:1331948/140‑1 (MQ=255)

                      ttACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACa                                                                                                      <  1:1207602/140‑1 (MQ=255)

                       tACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGTGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAAAGGAATCCCTGGCTTATTTTTCGAGTCCGAATTTACaa                                                                                                     >  1:609509/1‑140 (MQ=255)

                       tACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGt                                                                                                                 >  1:1394589/1‑128 (MQ=255)

                       tACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACaa                                                                                                     <  2:2086/140‑1 (MQ=255)

                       tACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACaa                                                                                                     >  1:1426154/1‑140 (MQ=255)

                        aCGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAat                                                                                                    <  2:994364/140‑1 (MQ=255)

                        aCGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAat                                                                                                    <  1:1010333/140‑1 (MQ=255)

                        aCGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAat                                                                                                    <  1:250032/140‑1 (MQ=255)

                          gTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAatat                                                                                                  <  1:825526/140‑1 (MQ=255)

                            tGACTTTATTGACAGACTTAACATTCACATTTCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCa                                                                                                <  2:734950/140‑1 (MQ=255)

                            tGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAat                                                                                                    <  1:1296685/136‑1 (MQ=255)

                            tGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAat                                                                                                    >  2:1296685/1‑136 (MQ=255)

                            tGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCa                                                                                                <  1:1200139/140‑1 (MQ=255)

                            tGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCa                                                                                                <  2:1521331/140‑1 (MQ=255)

                            tGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCa                                                                                                <  2:355556/140‑1 (MQ=255)

                            tGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCa                                                                                                <  2:392066/140‑1 (MQ=255)

                            tGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCa                                                                                                >  1:204311/1‑140 (MQ=255)

                             gACTTTATTGACAGACTTAACAATCGCATATCTTGAAATTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCAt                                                                                               <  1:649354/140‑1 (MQ=255)

                             gACTTTATTGACAGAATTAACATTCCCATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCAt                                                                                               <  1:620358/140‑1 (MQ=255)

                             gACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATGCTTGGCTGCTTATTGGAGTCCTAATTTACAATATCAt                                                                                               >  1:399048/1‑140 (MQ=255)

                             gACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCAt                                                                                               <  1:1295807/140‑1 (MQ=255)

                             gACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCAt                                                                                               >  1:818074/1‑140 (MQ=255)

                             gACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCAt                                                                                               <  1:1448804/140‑1 (MQ=255)

                             gACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCAt                                                                                               <  2:1167453/140‑1 (MQ=255)

                             gACTTTATTGACAGAATTAACACTCACATATTTTGTATTAAATGTCCATGATGTTGTTTCCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCAt                                                                                               <  2:446910/140‑1 (MQ=255)

                              aCTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATg                                                                                              <  1:1400575/140‑1 (MQ=255)

                                  tATTGACAGAATTAACATTCACCTATCTTCAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCata                                                                                          <  1:554959/140‑1 (MQ=255)

                                   aTTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACa                                                                                                      <  2:1263610/127‑1 (MQ=255)

                                   aTTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCatat                                                                                         >  2:1010673/1‑140 (MQ=255)

                                    ttGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCAtata                                                                                        <  2:278547/140‑1 (MQ=255)

                                    ttGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCAtata                                                                                        <  1:1478276/140‑1 (MQ=255)

                                     tGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAg                                                                                       <  2:900893/140‑1 (MQ=255)

                                     tGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAg                                                                                       <  1:883611/140‑1 (MQ=255)

                                         aGAATTAACATTCACATGTCTTTAATTTAATCTCCCTGACGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCa                                                                                   <  2:465821/140‑1 (MQ=255)

                                         aGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTCTTCGAGTCCGAGTTTACAATATCATGCATATAGACCa                                                                                   >  1:756199/1‑140 (MQ=255)

                                                aCATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGAc                                                                                     >  2:1458109/1‑131 (MQ=255)

                                                 cATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGa                                                                           >  2:1485409/1‑140 (MQ=255)

                                                     cacaTATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCt                                                                       >  1:307157/1‑140 (MQ=255)

                                                       caTATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGc                                                                     >  2:171157/1‑140 (MQ=255)

                                                        atatCTTGAATTTAATGTCCATAATGTTGTTTGCGTGAGAACATTCTCCGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCg                                                                    <  2:621379/140‑1 (MQ=255)

                                                           tCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCa                                                                                   <  2:789230/122‑1 (MQ=255)

                                                           tCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCa                                                                                   >  1:789230/1‑122 (MQ=255)

                                                            cTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGaaaa                                                                >  2:1347516/1‑140 (MQ=255)

                                                            cTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGaaaa                                                                >  1:1138182/1‑140 (MQ=255)

                                                              tGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCAAATAAACAATTTCAAGAATCTGCGAAAAtt                                                              >  1:554668/1‑140 (MQ=255)

                                                                aaTTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCa                                                            >  1:921080/1‑140 (MQ=255)

                                                                aaTTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCa                                                            >  1:1462106/1‑140 (MQ=255)

                                                                  tttAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAAt                                                          <  2:1204390/140‑1 (MQ=255)

                                                                  tttAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAAt                                                          <  2:23254/140‑1 (MQ=255)

                                                                  tttAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAAt                                                          >  2:196238/1‑140 (MQ=255)

                                                                    tAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGt                                                        <  2:921080/140‑1 (MQ=255)

                                                                    tAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGt                                                        <  1:1258128/140‑1 (MQ=255)

                                                                    tAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGCAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGt                                                        <  1:1347516/140‑1 (MQ=255)

                                                                    tAATGTCCATGATGTTGTTTGCGTGAGAACAATCTGAGCTTTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGt                                                        <  2:554668/140‑1 (MQ=255)

                                                                     aaTGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTc                                                       >  1:1540579/1‑140 (MQ=255)

                                                                     aaTGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTc                                                       <  1:1526209/140‑1 (MQ=255)

                                                                     aaTGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTc                                                       <  2:298266/140‑1 (MQ=255)

                                                                      aTGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTcc                                                      >  1:1224345/1‑140 (MQ=255)

                                                                           cATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCa                                                     >  1:604873/1‑136 (MQ=255)

                                                                           cATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCa                                                     <  2:604873/136‑1 (MQ=255)

                                                                            atgatgTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGt                                                        >  2:193977/1‑132 (MQ=255)

                                                                            atgatgTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGt                                                        <  1:193977/132‑1 (MQ=255)

                                                                                 gttgttTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCt                                           <  1:899336/140‑1 (MQ=255)

                                                                                    gttTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTcac                                        >  1:797389/1‑140 (MQ=255)

                                                                                     tttGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCa                                                     >  2:950382/1‑126 (MQ=255)

                                                                                     tttGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCa                                                     <  1:950382/126‑1 (MQ=255)

                                                                                       tGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACAtt                                     >  2:1412911/1‑140 (MQ=255)

                                                                                          gTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCt                                           >  1:268213/1‑131 (MQ=255)

                                                                                          gTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACAttttt                                  >  1:365210/1‑140 (MQ=255)

                                                                                          gTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCGGCGAAAACTCAGTGACCATTGAATACCTCACAttttt                                  >  1:392594/1‑140 (MQ=255)

                                                                                             agaACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTAcc                               <  1:1262494/140‑1 (MQ=255)

                                                                                                 cATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGa                           <  2:64522/140‑1 (MQ=255)

                                                                                                   ttCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACt                         <  2:917566/140‑1 (MQ=255)

                                                                                                      tcAGCATTAAGGAATTTTTTTACCGAAATCCTTGGCTGCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGa                      <  1:621495/140‑1 (MQ=255)

                                                                                                         gCATTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGt                   <  1:1470681/140‑1 (MQ=255)

                                                                                                           aTTAAGGAATTTTTTTATCGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTa                 <  2:591512/140‑1 (MQ=255)

                                                                                                           aTTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTa                 <  2:818074/140‑1 (MQ=255)

                                                                                                           aTTAAGGAATTTTTTTAACGGAATCCTTGGCTTCTCTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTa                 <  2:524954/140‑1 (MQ=255)

                                                                                                            ttAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTAc                >  1:809594/1‑140 (MQ=255)

                                                                                                            ttAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGGATACCTCACACTTTTACCGTGACTCGATGTTAc                >  2:677143/1‑140 (MQ=255)

                                                                                                             tAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACt               >  2:307311/1‑140 (MQ=255)

                                                                                                             tAAGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACt               <  1:983330/140‑1 (MQ=255)

                                                                                                              aaGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGa                           <  2:620093/127‑1 (MQ=255)

                                                                                                               aGGAATTTTTTTAACGGCATCCCTGGCTTCTCTTTAGAGCCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGt             <  2:392594/140‑1 (MQ=255)

                                                                                                               aGGAATTTTTTTAACGGAATCCTTGGCTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTGAATGTCCAGTGTATACATCACATTTGTACCGTGACTCGATGTTACTGt             >  2:424049/1‑140 (MQ=255)

                                                                                                                       ttttAACGGAATCCTTGGCTTCCTTCTCGTGTCCGAAGTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCaataat     <  1:424049/140‑1 (MQ=255)

                                                                                                                       ttttAACGGAATCCTTGCCTTCCTTTTCGAGGCCGCATTCACAATATCCTGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCaataat     <  2:404673/140‑1 (MQ=255)

TAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCAC  >  NC_000913/2990149‑2990410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`