Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	427,546	A→G	D87G (GAC→GGC)	yajC →	Sec translocon accessory complex subunit YajC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	427,546	0	A	G	100.0%	241.7 / NA	67	D87G (GAC→GGC)	yajC	Sec translocon accessory complex subunit YajC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/32);  total (35/32)

ACAGCAGAAGCGCACCAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTACG  >  NC_000913/427409‑427677                                                                                                                                          |                                                                                                                                    acaGCAGAAGCGCACCAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGGTGAATGgca                                                                                                                                   >  2:246265/1‑140 (MQ=255)  cagcagAAGCGCACCAAAGAACTCAAAAAGCCGATGGACCCCATTGCCTAAGGTGATGAAGTTCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGgcac                                                                                                                                  <  2:569007/140‑1 (MQ=255)  cagcagAAGCGCACCAAAGAACACAAAAAGCTGATAGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGgcac                                                                                                                                  >  1:1036317/1‑140 (MQ=255)  cagcagAAGCGCACCAAAGAACACAAAAAACTGATGGCCTCCATTGCCAAAGGTGATGAAGTTCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGgcac                                                                                                                                  <  1:608287/140‑1 (MQ=255)   agcagAAGCGCACCAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTTGCGTAAACCGGCTACATTGCTTTCGCGCTGAATGGcacc                                                                                                                                 >  2:608287/1‑140 (MQ=255)    gcagAAGCGCACCAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGGcacca                                                                                                                                <  1:893579/140‑1 (MQ=255)      agAAGCGCACCAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGGCACCACt                                                                                                                              <  1:1309520/140‑1 (MQ=255)        aaGCGCACCAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTAGATTACTCTCGCGCCGAATGGCACCACTGa                                                                                                                            >  1:663569/1‑140 (MQ=255)          gcgcACCAAAGAACACGAAAAGTTGATGGACTCAATTGCCAAAGGTGATGAAGTTCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGGCACCACTGAag                                                                                                                          <  2:708809/140‑1 (MQ=255)          gcgcACCAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGGCACCACTGAag                                                                                                                          <  1:52081/140‑1 (MQ=255)          gcgcACCAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGGCACCACTGAag                                                                                                                          <  1:765414/140‑1 (MQ=255)          gcgcACCAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGGCACCACTGAag                                                                                                                          <  2:57443/140‑1 (MQ=255)          gcgcACCAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGGCACCACTGAag                                                                                                                          <  1:1033388/140‑1 (MQ=255)             cACCAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGGCACCACTGAagtag                                                                                                                       >  1:48367/1‑140 (MQ=255)               ccAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAACGGTGGCGTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGgcac                                                                                                                                  <  2:6276/127‑1 (MQ=255)               ccAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGGCACCACTGAag                                                                                                                          >  2:777394/1‑135 (MQ=255)               ccAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGGCACCACTGAag                                                                                                                          <  1:777394/135‑1 (MQ=255)               ccAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGAGATGAAGTTCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGgcac                                                                                                                                  >  1:6276/1‑127 (MQ=255)                   aGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGGCACCACTGAAGTAGttatta                                                                                                                 <  2:48367/140‑1 (MQ=255)                          aaaaaGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGGCACCACTGAAGTAGTTATTAAACg                                                                                                             >  2:1166049/1‑137 (MQ=255)                          aaaaaGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGGCACCACTGAAGTAGTTATTAAACg                                                                                                             <  1:1166049/137‑1 (MQ=255)                            aaaGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGGCACCACTGAAGTAGTTATTAAACGTGACt                                                                                                        >  1:1288533/1‑140 (MQ=255)                                  gATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGCAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGGCACCACTGAAGTAGTTATTAAACGTGACTTCGTAg                                                                                                  >  1:230937/1‑140 (MQ=255)                                           cATTGCCAAAGGTGATGAAGTTCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGGCACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTcc                                                                                         <  2:225532/140‑1 (MQ=255)                                              tGCCAAAGGTGATGAAGTTCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGGCACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGc                                                                                      >  1:1187458/1‑140 (MQ=255)                                                     ggTGATGAAGTTCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAGTGGCACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAgg                                                                               >  2:927374/1‑140 (MQ=255)                                                     ggTGATGAAGTTCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGGCACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCTGTCCTGCCGAAAgg                                                                               >  1:1454756/1‑140 (MQ=255)                                                          tgaAGTTCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGGCACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCa                                                                          >  2:252935/1‑140 (MQ=255)                                                          tgaAGTTCTGACGAACGCTGGCATGGTTGGTCGCGAAACAAAAGTAGCCGAAAACGGCTACATTGCTATCGCGCTGAATGGCACCACTGATGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCa                                                                          <  2:456054/140‑1 (MQ=255)                                                               ttCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGGCACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAg                                                                     >  2:297235/1‑140 (MQ=255)                                                                tCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGGCACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAgg                                                                    >  1:307316/1‑140 (MQ=255)                                                                       aaCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGGCACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTa                                                             >  2:141534/1‑140 (MQ=255)                                                                        aCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGGCACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGGCCTGCCGAGAGGCACCATGAAGGCGCTGTaa                                                            >  2:566059/1‑140 (MQ=255)                                                                         cGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGGCACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAAt                                                           >  2:1062761/1‑140 (MQ=255)                                                                         cGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGGCACCACTGAAGTAGTTATTAAACGTGACTTCGTACCTGTCGTCCTGCCGAACGGCAGCATGAAGGCGCTGTAAt                                                           >  2:479688/1‑140 (MQ=255)                                                                              gCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGGCACCACTGAAGTAGTTATTAAACGTGACTTTGTAGCTGCCGTCGTGCAGAAAGGCACCATGGAGGCGCTGTAATTaaaa                                                      >  2:498656/1‑140 (MQ=255)                                                                               ccTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGGCACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAAt                                                     <  2:1288533/140‑1 (MQ=255)                                                                                      ggtCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGGCACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTccc                                              >  1:1313274/1‑140 (MQ=255)                                                                                       gtCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGGCACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCt                                             <  1:844646/140‑1 (MQ=255)                                                                                        tCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGGCACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAATGGCACCATGAACGCACTGTAAGTAAAATTTTTCCCTa                                            >  1:729536/1‑140 (MQ=255)                                                                                        tCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGGCACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTa                                            >  1:235563/1‑140 (MQ=255)                                                                                         cgcgTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGGCACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAATGCACCATTAAGGCGGTGTAATAAAAATTTTTCCCTaa                                           >  1:523741/1‑140 (MQ=255)                                                                                             tAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGGCACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGa                                       >  1:63340/1‑140 (MQ=255)                                                                                             tAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGGCACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGa                                       <  1:252935/140‑1 (MQ=255)                                                                                              aaCCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGGCACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGaa                                      >  2:863973/1‑140 (MQ=255)                                                                                                 cAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGGCACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTg                                   <  2:63340/140‑1 (MQ=255)                                                                                                  aaaGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGGCACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGc                                  <  1:1062761/140‑1 (MQ=255)                                                                                                         cGGAAAACGGCTACATTTCTATCGCGCTGAATGGCACCACTGAAGTAGATATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTa                           <  2:445231/140‑1 (MQ=255)                                                                                                         cGGAAAACGGCTACATTGCTATCGCGCTGAATGGCACCACTTAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTa                           >  1:1431144/1‑140 (MQ=255)                                                                                                         cGGAAAACGGCTACATTGCTATCGCGCTGAATGGCACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTa                           <  2:1187458/140‑1 (MQ=255)                                                                                                         cGGAAAACGGCTACATTGCTATCGCGCTGAATGGCACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTa                           >  1:1420649/1‑140 (MQ=255)                                                                                                         cGGAAAACGGCTACATTGCTATCGCGCTGAATGGCACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTa                           <  2:1313274/140‑1 (MQ=255)                                                                                                         cGGAAAACGGCTACATTGCTATCGCGCTGAATGGCACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTa                           >  2:1318894/1‑140 (MQ=255)                                                                                                         cGGAAAACGGCTACATTGCTATCGCGCTGAATGGCACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCAGTAATTAAAATGTTTCCCTAAAGGAATTGCCGTGTTa                           >  1:453240/1‑140 (MQ=255)                                                                                                          ggAAAACGGCTACATTGCTATCGCGCTGAATGGCACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTaa                          >  1:212164/1‑140 (MQ=255)                                                                                                              aaCGGCTACATTGCTATCGCGCTGAATGGCACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCg                      >  1:179140/1‑140 (MQ=255)                                                                                                               aCGGCTACATTGCTATCGCGCTGAATGGCACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGt                     <  2:1204625/140‑1 (MQ=255)                                                                                                                cGGCTACATTGCTATTGCGCTGTATCGCAGCACTGAACTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGtt                    <  1:479688/140‑1 (MQ=255)                                                                                                                cGGCTACATTGCTATCGCGCTGAATGGCACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGtt                    <  2:1176829/140‑1 (MQ=255)                                                                                                                   cTACATTGCTATCGCGCTGAATGGCACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATc                 <  2:230937/140‑1 (MQ=255)                                                                                                                   cTACATTGCTATCGCGCTGAATAGCACCACTGAAGTAGTTATTAAACGGGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATc                 <  2:663569/140‑1 (MQ=255)                                                                                                                          gCTATCGCGCTGAATGGCACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTtgtg          <  1:297235/140‑1 (MQ=255)                                                                                                                           cTATCGCGCTGAATGGCACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTgg         >  1:258177/1‑140 (MQ=255)                                                                                                                             aTCGCGGTGAATGGCACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGaa       <  1:246265/140‑1 (MQ=255)                                                                                                                               cgcgCTGAATGGCACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCTCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGt     <  2:1348173/140‑1 (MQ=255)                                                                                                                               cgcgCTGAATGGCACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGt     <  2:307316/140‑1 (MQ=255)                                                                                                                               cgcgCTGAATGGCACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGt     <  1:863973/140‑1 (MQ=255)                                                                                                                                gcgcTGAATGGCACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTa    >  2:846698/1‑140 (MQ=255)                                                                                                                                gcgcTGAATGGCACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATATTTCCCTAGGGGCATTGCCGTGTTAAACCGGTATCCTTTGTGGAAGTa    >  2:471558/1‑140 (MQ=255)                                                                                                                                 cgcTGAATGGCACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTAc   >  1:1502280/1‑140 (MQ=255)                                                                                                                                  gcTGAATGGCACCACCGAAGTACTTCTTACACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTACg  <  1:498656/140‑1 (MQ=255)                                                                                                                                   cTGAATGGCACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGaa       >  1:130532/1‑134 (MQ=255)                                                                                                                                   cTGAATGGCACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGaa       <  2:130532/134‑1 (MQ=255)                                                                                                                                          |                                                                                                                                    ACAGCAGAAGCGCACCAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTACG  >  NC_000913/427409‑427677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑