Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,076,176	A→G	S3P (TCC→CCC)	yggF ←	fructose 1,6‑bisphosphatase YggF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,076,176	0	A	G	100.0%	214.7 / NA	60	S3P (TCC→CCC)	yggF	fructose 1,6‑bisphosphatase YggF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/33);  total (27/33)

AGCGACGTCGTTTAATGCCTGGCGCATCGCGGTGACCGCCAGGCCATCAATTTTATTTTTGTCGCCACATCCGGTTTGCGGCCAGGCAGCCAACGCTGCCTGTTCCGTAACGCGAAATAATGGCCACGCCAGGGACATCATTGCGCGGCCTCCCCGATATCAACGCCAAATTGTGCCAGCAGATATTTCTCGGCCTGTTCATTCCAGATGCCGTGCGTTTCCTCAACCAGCCGCGCCAACTCTTTAAACAGCGGATCGGTTTTGCCT  >  NC_000913/3076042‑3076308                                                                                                                                       |                                                                                                                                     aGCGACGTCGTTTAATGCCTGGCGCATCGCGGTGACCGCCAGGCCATCAATTTTATTTTTGTCGCCACATCCGGTTTGCGGCCAGGCAGCCAACGCTGCCTGTTCCGTAACGCGAAATAATGGCCACGCCAGGGGcatca                                                                                                                                 >  2:1193562/1‑140 (MQ=255)          gtTTAATGCCTGGCGCTTCGCGTTGACCGCCAGGCTATCAATTTTATTATTGTCGCCACATCCGGTTTGCGGCCAGGCAGCCAACGCTGCCTGTTCCGTAACGCGAAATAATGGCCACGCCAGGGGCATCATTGCGCGGc                                                                                                                        <  2:690508/140‑1 (MQ=255)           tttAATGCCTGGCGCATTGCGGTGACCGCCATGCCCTCATTTTTATTTATGTCGCCACATCCGGTTTGCGGCCAGGCAGCCAACGCTGCCTGTTCCGTAACGCGAAATAATGGCCACGCCAGGGGCATCATTGCGCGGcc                                                                                                                       <  2:479449/140‑1 (MQ=255)           tttAATGCCTGGCGCATCGCGGTGACCGCCAGGCCATCAATTTTATTTTTGTCGCCACATCCGGTTTGCGGCCAGGCAGCCAACGCTGCCTGTTCCGTAACGCGAAATAATGGCCACGCCAGGGGCATCATTGCGCGGcc                                                                                                                       <  2:128509/140‑1 (MQ=255)                     ggctcATCGCGGTGACCGCCAGGCCATCAATTTTATTTTTGTCGCCACATCCGGTTTGCGGCCAGGCAGCCAACGCTGCCTGTTCCGTAACGCGAAATAATGGCCACGCCAGGGGCATCATTGCGCGGCCTCCCCGatat                                                                                                             >  1:1318653/5‑140 (MQ=255)                     ggCGCATCGCGGTGACCGCCAGGCCATCAATTTTATTTTTGTCGCCACATCCGGTTTGCGGCCAGGCAGCCAACGCTGCCTGTTCCGTAACGCGAAATAATGGCCACGCCAGGGGCATCATTGCGCGGcc                                                                                                                       >  2:246592/1‑130 (MQ=255)                     ggCGCATCGCGGTGACCGCCAGGCCATCAATTTTATTTTTGTCGCCACATCCGGTTTGCGGCCAGGCAGCCAACGCTGCCTGTTCCGTAACGCGAAATAATGGCCACGCCAGGGGCATCATTGCGCGGcc                                                                                                                       <  1:246592/130‑1 (MQ=255)                     ggCGCATCGCGGTGACCGCCAGGCCATCAATTTTATTTTTGTCGCCACATCCGGTTTGCGGCCAGGCAGCCAACGCTGCCTGTTCCGTAACGCGAAATAATGGCCACGCCAGGGGCATCATTGCGCGGCCTCCCCGatat                                                                                                             >  1:767091/1‑140 (MQ=255)                     ggCGCATCGCGGTGACCGCCAGGCCATCAATTTTATTTTTGTCGCCACATCCGGTTTGCGGCCAGGCAGCCAACGCTGCCTGTTCCGTAACGCGAAATAATGGCCACGCCAGGGGCATCATTGCGCGGCCTCCCCGatat                                                                                                             >  2:284110/1‑140 (MQ=255)                     ggCGCATCGCGGTGACCGCCAGGCCATCAATTTTATTTTTGTCGCCACATCCGGTTTGCGGCCAGGCAGCCAACGCTGCCTGTTCCGTAACGCGAAATAATGGCCACGCCAGGGGCATCATTGCGCGGCCTCCCCGatat                                                                                                             <  2:1385004/140‑1 (MQ=255)                      gtgcATCGAGGTGACCATCAGGCCATCAATTATTTTTTTTTCGCCACAACCGGTTTGCGGCCAGGCAGCCAACGCTGCCTGTTCCGTAACGCGAAATAATGGCCACGCCAGGGGCATCATTGCGCGGCCTCCCCGATATc                                                                                                            <  2:698098/138‑1 (MQ=255)                       cgcATCGCGGTGACCCCCAGGCCATCAATTTTATTTTCGTCGCCACATCCGGTTTGCGGCCAGGCAGCCAACGCTGCCTGTTCCGTAACGCGAAATAATGGCCACGCCAGGGGCATCATTGCGCGGCCTCCCCGATATCa                                                                                                           <  1:625267/140‑1 (MQ=255)                          aTCGCGGTGACCGCCAGGCCATCAATTTTATTTTTGTCGCCACATCCGGTTTGCGGCCAGGCAGCCAACGCTGCCTGTTCCGTAACGCGAAATAATGGCCACGCCAGGGGCATCATTGCGCGGCCTCCCCGATATCAACg                                                                                                        <  1:1044657/140‑1 (MQ=255)                          aTCGCGGTGACCGCCAGGCCATCAATTTTATTTTTGTCGCCACATCCGGTTTGCGGCCAGGCAGCCAACGCTGCCTGTTCCGTAACGCGAAATAATGGCCACGCCAGGGGCATCATTGCGCAGCCTCCCCGATATCAACg                                                                                                        >  2:1143066/1‑140 (MQ=255)                           tCGCGGTGACCGCCAGGCCATCAATTTTATTTTTGTCGCCACATCCGGTTTGCGGCCAGGCAGCCAACGCTGCCTGTTCCGTAACGCGAAATAATGGCCACGCCAGGGGCATCATTGCGCGGCCTCCCCGATATCAACGc                                                                                                       <  1:1408534/140‑1 (MQ=255)                           tCGCGGTGACCGCCAGGCCATCAATTTTATTTTTGTCGCCACATCCGGTTTGCGGCCAGGCAGCCAACGCTGCCTGTTCCGTAACGCGAAATAATGGCCACGCCAGGGGCATCATTGCGCGGCCTCCCCGATATCAACGc                                                                                                       >  2:172641/1‑140 (MQ=255)                           tCGCGGTGACCGCCAGGCCATCAATTTTATTTTTGTCGCCACATCCGGTTTGCGGCCAGGCAGCCAACGCTGCCTGTTCCGTAACGCGAAATAATGGCCACGCCAGGGGCATCATTGCGCAGCCTCCCCGATATCAACGc                                                                                                       <  1:1143066/140‑1 (MQ=255)                            cgcgGTGACCGCCAGGCCATCAATTTTATTTTTGTCGCCACATCCGGTTTGCGGCCAGGCAGCCAACGCTGCCTGTTCCGTAACGCGAAATAATGGCCACGCCAGGGGCATCATTGCGCGGCCTCCCCGatat                                                                                                             <  1:901411/133‑1 (MQ=255)                            cgcgGCGACCGCCAGGCCATCAATTTTATTTTTGTCGCCACATCCGGTTTGCGGCCAGGCAGCCAACGCTGCCTGTTCCGTAACGCGAAATAATGGCCACGTCAGGGGCATCATTGCGCGGCCTCCCCGatat                                                                                                             >  2:901411/1‑133 (MQ=255)                                       ccAGGCCATCAATTTTATTTTTGTCGCCACATCCGGTTTGCGGCCAGGCAGCCAACGCTGCCTGTTCCGTAACGCGAAATAATGGCCACGCCAGGGGCATCATTGCGCGGCCTCCCCGATATCAACGCCAAATTGTGCca                                                                                           >  1:782797/1‑140 (MQ=255)                                        cAGGCCATCAATTTTATTTTTTTCGCCACATCCGGTTTGCGGTCATGCAACCAACGCTTCCTGTTCCGTAACGCGAAATAATGGCCACGCCAGGGGCATTATTGCGCGGCCTCCCCGATTACAACGCCAAATTTTGCcag                                                                                          >  2:1332733/1‑140 (MQ=255)                                               tCAATTTTATTTTTGTCGCCACATCCGGTTTGCGGCCAGGCAGCCAACGCTGCCTGTTCCGTAACGCGAAATAATGGCCACGCCAGGGGCATCATTGCGCGGCCTCCCCGATATCAACGCCAAATTGTGCCAGCAGat                                                                                     <  1:351766/138‑1 (MQ=255)                                               tCAATTTTATTTTTGTCGCCACATCCGGTTTGCGGCCAGGCAGCCAACGCTGCCTGTTCCGTAACGCGAAATAATGGCCACGCCAGGGGCATCATTGCGCGGCCTCCCCGATATCAACGCCAAATTGTGCCAGCAGat                                                                                     >  2:351766/1‑138 (MQ=255)                                               tCAATTTTATTTTTGTCGCCACATCCGGTTTGCGGCCAGGCAGCCAACGCTGCCTGTTCCGTAACGCGAAATAATGGCCACGCCAGGGGCAACATTGCGCAGCCCCCCTGATATCAAAGCCAAATTGTGCCAGCAGatat                                                                                   >  2:510294/1‑140 (MQ=255)                                                  attttattttTGTCGCCACATCCGGTTTGCGGCCAGGCAGCCAACGCTGCCTGTTCCGTAACGCGAAATAATGGCCACGCCAGGGGCATCATTGCGCGGCCTCCCCGATATCAACGCCAAATTGTGCCAGCAGATATTtc                                                                                <  1:1332733/140‑1 (MQ=255)                                                  attttattttTGTCGCCACATCCGGTTTGCGGCCAGGCAGCCAACGCTGCCTGTTCCGTAACGCGAAATAATGGCCACGCCAGGGGCATCATTGCGCGGCCTCCCCGATATCAACGCCAAATTGTGCCAGCAGATATTtc                                                                                <  2:1415525/140‑1 (MQ=255)                                                  attttattttTGTCGCCACATCCGGTTTGCGGCCAGGCAGCCAACGCTGCCTGTTCCGTAACGCGAAATAATGGCCACGCCAGGGGCATCATTGCGCGGCCTCCCCGATATCAACGCCAAATTGTGCCAGCAGATATTtc                                                                                <  2:782797/140‑1 (MQ=255)                                                       attttTGTCGCCACATCCGGTTTGCGGCCAGGCAGCCAACGCTGCCTGTTCCGTAACGCGAAATAATGGCCACGCCAGGGGCATCATTGCGCGGCCTCCCCGATATCAACGCCAAATTGTGCCAGCAGATATTTCTCGGc                                                                           <  1:510294/140‑1 (MQ=255)                                                       attttTGTCGCCACATCCGGTTTGCGGCCAGGCAGCCAACGCTGCCTGTTCCGTAACGCGAAATAATGGCCACGCCAGGGGCATCATTGCGCGGCCTCCCCGATATCAACGCCAAATTGTGCCAGCAGATATTTCTCGGc                                                                           <  2:1287368/140‑1 (MQ=255)                                                       attttTGTCGCCACATCCGGTTTGCGGCCAGGCAGCCAACGCTGCCTGTTCCGTAACGCGAAATAATGGCCACGCCAGGGGCATCATTGCGCGGCCTCCCCGATATCAACGCCAAATTGTGCCAGCAGATATTTCTCGGc                                                                           <  1:284110/140‑1 (MQ=255)                                                             gTCGCCACATCCGGTTTGCGGCCAGGCAGCCAACGCTGCCTGTTCCGTAACGCGAAATAATGGCCACGCCAGGGGCATCATTGCGCGGCCTCCCCGATATCAACGCCAAATTGTGCCAGCAGat                                                                                     <  2:1474825/124‑1 (MQ=255)                                                             gTCGCCACATCCGGTTTGCGGCCAGGCAGCCAACGCTGCCTGTTCCGTAACGCGAAATAATGGCCACGCCAGGGGCATCATTGCGCGGCCTCCCCGATATCAACGCCAAATTGTGCCAGCAGat                                                                                     >  1:1474825/1‑124 (MQ=255)                                                             gTCGCCACATCCGGTTTGCGGCCAGGCAGCCAACGCTGCCTGTTCCGTAACGCGAAATAATGGCCACGCCAGGGGCATCATTGCGCGGCCTCCCCGATATCAACGCCAAATTGTGCCAGCAGATATTTCTCGGCCTGTTc                                                                     >  1:1238344/1‑140 (MQ=255)                                                             gTCGCCACATCCGGTTTGCGGCCAGGCAGCCAACGCTGCCTGTTCCGTAACGCGAAATAATGGCCACGCCAGGGGCATCATTGCGCGGCCTCCCCGATATCAACGCCAAATTGTGCCAGCAGATATTTCTCGGCCTGTTc                                                                     >  2:929928/1‑140 (MQ=255)                                                             gTCGCCACATCCGGTTTGCGGCCAGGCAGCCAACGCTGCCTGTTCCGTAACGCGAAATAATGGCCACGCCAGGGGCATCATTGCGCGGCCTCCCCGATATCAACGCCAAAATGTACCAGCAGATGTTTCTCGGCCTGTTc                                                                     >  1:650469/1‑140 (MQ=255)                                                                gCCACATCCGGTTTGCGGCCAGGCAGCCAACGCTGCCTGTTCCGTAACGCGAAATAATGGCCACGCCAGGGGCATCATTGCGCGGCCTCCCCGATATCAACGCCAAATTGTGCCAGCAGATATTTCTCGGCCTGTTCAtt                                                                  >  2:47085/1‑140 (MQ=255)                                                                   acaTCCGGTTTGCGGCCAGGCAGCCAACGCTGCCTGTTCCGTAACGCGAAATAATGGCCACGCCAGGGGCATCATTGCGCGGCCTCCCCGATATCAACGCCAGATTGTGCGAGCGGAGATTTCTAGGCCTGTTCATTCCa                                                               >  1:450906/1‑140 (MQ=255)                                                                              gCGGCCAGGCAGCCAACGCTGCCTGTTCCGTAACGCGAAATAATGGCCACGCCAGGGGCATCATTGCGTGGCCTCCCCGATATCAACGCCAAATTGTGCCAGCAGATATTTCTCGGCCTGTTCATTCCAGATGCCGTGCg                                                    <  1:925185/140‑1 (MQ=255)                                                                               cGGCCAGGCAGCCAACGCTGCCTGTTCCGTAACGCGAAATAATGGCCACGCCAGGGGCATCATTGCGCGGCCTCCCCGATATCAACGCCAAATTGTGCCAGCAGATATTTCTCGGCCTGTTCATTCCAGATGCCGTGCGt                                                   >  2:1239489/1‑140 (MQ=255)                                                                               cGGCCAGGCAGCCAACGCTGCCTGTTCCGTAACGCGAAATAATGGCCACGCCAGGGGCATCATTGCGCGGCCTCCCCGATATCAACGCCAAATTGTGCCAGCAGATATTTCTCGGCCTGTTCATTCCAGATGCCGTGCGt                                                   >  1:924704/1‑140 (MQ=255)                                                                                         gCCAACGCTGCCTGTTCCGTAACGCGAAATAATGGCCACGCCAGGGGCATCATTGCGCGGCCTCCCCGATATCAACGCCAAATTGTGCCAGCAGATATTTCTCGGCCTGTTCATTCCAGATGCCGTGCGTTTCCTCAAcc                                         >  2:113424/1‑140 (MQ=255)                                                                                            aaCGCTGCCTGTTCCGTAACGCGAAATAATGGCCACGCCAGGGGCATCATTGCGCGGCCTCCCCGATATCAACGCCAAATTGTGCCAGCAGATATTTCTCGGCCTGTTCATTCCAGATGCCGTGCGTTTCCTCAACCAGc                                      <  1:1239489/140‑1 (MQ=255)                                                                                                   ccTGTTCCGTAACGCGAAATAATGGCCACGCCAGGGGCATCATTGCGCGGCCTCCCCGATATCAACGCCAAATTGTGCCAGCAGATATTTCTCGGCCTGTTCATTCCAGATGCCGTGCGTTTCCTCAACCAGCCGCGCCa                               <  2:1318653/140‑1 (MQ=255)                                                                                                   ccTGTTCCGTAACGCGAAATAATGGCCACGCCAGGGGCATCATTGCGCGGCCTCCCCGATATCAACGCCAAATTGTGCCAGCAGATATTTCTCGGCCTGTTCATTCCAGATGCCGTGCGTTTCCTCAACCAGCCGCGCCa                               <  1:200500/140‑1 (MQ=255)                                                                                                   ccTGTTCCGTAACGCGAAATAATGGCCACGCCAGGGGCATCATTGCGCGGCCTCCCCGATATCAACGCCAAATTGTGCCAGCAGATATTTCTCGGCCTGTTCATTCCAGATGCCGTGCGTTTCCTCAACCAGCCGCGCCa                               <  1:1267930/140‑1 (MQ=255)                                                                                                   ccTGTTCCGTAACGCGAAATAATGGCCACGCCAGGGGCATCATTGCGCGGCCTCCCCGATATCAACGCCAAATTGTGCCAGCAGATATTTCTCGGCCTGTTCATTCCAGATGCCGTGCGTTTCCTCAACCAGCCGCGCCa                               <  2:324866/140‑1 (MQ=255)                                                                                                   ccTGTTCCGTAACGCGAAATAATGGCCACGCCAGGGGCATCATTGCGCGGCCTCCCCGATATCAACGCCAAATTGTGCCAGCAGATATTTCTCGGCCTGTTCATTCCAGATGCCGTGCGTTTCCTCAACCAGCCGCGCCa                               <  1:1193562/140‑1 (MQ=255)                                                                                                   ccTGTTCCGTAACGCAAAATAATGGCCACCCCAGGGGAATCATTGCGCGGCCTCCCCGATATCAACGCCAAATTGTGCCAGCAGATATTTCTCGGCCTGTTCATTCCAGATGCCGTGCGTTTCCTCAACCAGCCGCGCCa                               <  1:500714/140‑1 (MQ=255)                                                                                                    cTGTTCCGTAACGCGAAATAATGGCCACGCCAGGGGCATCATTGCGCGGCCTCCCCGATATCAACGCCAAATTGTGCCAGCAGATATTTCTCGGCCTGTTCATTCCAGATGCCGTGCGTTTCCTCAACCAGCCGCGCCaa                              <  1:929928/140‑1 (MQ=255)                                                                                                    cTGTTCCGTAACGCGAAATAATGGCCACGCCAGGGGCATCATTGCGCGGCCTCCCCGATATCAACGCCAAATTGTGCCAGCAGATATTTCTCGGCCTGTTCATTCCAGATGCCGTGCGTTTCCTCAACCAGCCGCGCCaa                              >  2:799831/1‑140 (MQ=255)                                                                                                        tCCGTAACGCGAAATAATGGCCACGCCAGGGGCATCATTGCGCGGCCTCCCCGATATCAACGCCAAATTGTGCCAGCAGATATTTCTCGGCATGTTCATTTCAGATGCAGTGCGTTTACTCATCCAGCCGCGCCAActct                          >  2:410935/1‑140 (MQ=255)                                                                                                             aaCGCGAAATAATGGCCACGCCAGGGGCATCATTGCGCGGCCTCCCCGATATCAACGCCAAATTGTGCCAGCAGATATTTCTCGGCCTGTTCATTCCAGATGCCGTGCGTTTCCTCAACCAGCCGCGCCAACTCTTTaaa                     >  2:152764/1‑140 (MQ=255)                                                                                                              aCGCGAAATAATGGCCACGCCAGGGGCATCATTGCGCGGCCTCCCCGATATCAACGCCAAATTGTGCCAGCAGATATTTCTCGGCCTGTTCATTCCAGATGCCGTGCGTTTCCTCAACCAGCCGCGCCAACTCTTTAAAc                    <  1:113424/140‑1 (MQ=255)                                                                                                               cgcgAAATAATGGCCACGCCAGGGGCATCATTGCGCGGCCTCCCCGATATCAACGCCAAATTGTGCCAGCAGATATTTCTCGGCCTGTTCATTCCAGATGCCGTGCGTTTCCTCAACCAGCCGCGCCAACTCTTTAAACa                   >  2:1446154/1‑140 (MQ=255)                                                                                                                 cgAAATAATGGCCACGCCAGGGGCATCATTGCGCGGCCTCCCCGATATCAACGCCAAATTGTGCCAGCAGATATTTCTCGGCCTGTTCATTCCAGATGCCGTGCGTTTCCTCAATCATCCGCGCTAACTCTTTAAACAGc                 >  1:709163/1‑140 (MQ=255)                                                                                                                 cgAAATAATGGCCACGCCAGGGGCATCATTGCGCGGCCTCCCCGATATCAACGCCAAATTGTGCCAGCAGATATTTCTCGGCCTGTTCATTCCAGATGCCGTGCGTTTCCTCAACCAGCCGCGCCa                               >  1:1094861/1‑126 (MQ=255)                                                                                                                 cgAAATAATGGCCACGCCAGGGGCATCATTGCGCGGCCTCCCCGATATCAACGCCAAATTGTGCCAGCAGATATTTCTCGGCCTGTTCATTCCAGATGCCGTGCGTTTCCTCAACCAGCCGCGCCa                               <  2:1094861/126‑1 (MQ=255)                                                                                                                    aataatGGCCACGCCAGGGGCATCATTGCGCGGCCTCCCCGATATCAACGCCAAATTGTGCCAGCAGATATTTCTCGGCCTGTTCATTCCAGATGCCGTGCGTTTCCTCAACCAGCCGCGCCAACTCTTTAAACAGCGGa              <  1:1446154/140‑1 (MQ=255)                                                                                                                    aataatGGCCACGCCAGGGGCATCACTGCGCGGCCTCCCCGATATCAACGCCAAATTGTGCCAGCAGATATTTCTCGGCCTGTTCATTCCAGATGCCGTGCGTTTCCTCAACCAGCCGCGCCAACTCTTTAAACAGCGGa              <  1:615457/140‑1 (MQ=255)                                                                                                                     ataatGGCCACGCCAGGGGCATCATTGCGCGGCCTCCCCGATATCAACGCCAAATTGTGCCAGCAGATATTTCTCGGCCTGTTCATTCCAGATGCCGTGCGTTTCCTCAACCAGCCGCGCCAACTCTTTAAACAGTGGAt             <  1:47085/140‑1 (MQ=255)                                                                                                                                gCCAGGGGCATCATTGCGCGGCCTCCCCGATATCAACGCCAAATTGTGCCAGCAGATATTTCTCGGCCTGTTCATTCCAGATGCCGTGCGTTTCCTCAACCAGCCGCGCCAACTCTTTAAACAGCGGATCGGTTTTGCCt  >  1:94180/1‑140 (MQ=255)                                                                                                                                gCCAGGGGCATCATTGCGCGGCCTCCCCGATATCAACGCCAAATTGTGCCAGCAGATATTTCTCGGCCTGTTCATTCCAGATGCCGTGCGTGTCCTCAACGAGCTGCGGCAACTCTTCAAACAGCGGATCGGTTTTGCCt  >  2:419082/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                     AGCGACGTCGTTTAATGCCTGGCGCATCGCGGTGACCGCCAGGCCATCAATTTTATTTTTGTCGCCACATCCGGTTTGCGGCCAGGCAGCCAACGCTGCCTGTTCCGTAACGCGAAATAATGGCCACGCCAGGGACATCATTGCGCGGCCTCCCCGATATCAACGCCAAATTGTGCCAGCAGATATTTCTCGGCCTGTTCATTCCAGATGCCGTGCGTTTCCTCAACCAGCCGCGCCAACTCTTTAAACAGCGGATCGGTTTTGCCT  >  NC_000913/3076042‑3076308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑