Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,133,542	G→A	P139L (CCG→CTG)	yghS ←	putative ATP‑binding protein YghS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,133,542	0	G	A	100.0%	284.6 / NA	87	P139L (CCG→CTG)	yghS	putative ATP‑binding protein YghS
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (46/41);  total (46/41)

AAACGCGGTTTGTTCATCAATGCCAAGACGAATCAACAATACGGGCAAATAAGATGCCATCCATTGGTACAGCTTCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCGGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGCGGTCGGTGATGAGCAGAAAGCCTTGCTGGCTTTTACACAACATTTTGCGAAACTTGTACGCCCGCCAGCAGGAAAGCAGATAGATAACCAGTG  >  NC_000913/3133406‑3133677                                                                                                                                         |                                                                                                                                        aaaCGCGGTTTGTTCATCAATGCCAAGACGAATCAACAATACGGGCAAATAAGATGCCATCCATTGGTACAGCTTCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGc                                                                                                                                      >  2:877347/1‑140 (MQ=255)         tttGTTCATCAATGCCAAGACGAATCAACAATACGGGCAAATAAGATGCCATCCATTGGTACAGCTTCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAg                                                                                                                              <  1:1465570/140‑1 (MQ=255)            gTTCATCAATGCCAAGACGAATCAACAATACGGGCAAATAAGATGCCATCCATTGGTACAGCTTCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGa                                                                                                                           <  1:979821/140‑1 (MQ=255)                   aaTGCCAAGACGAATCAACAATACGGGCAAATAAGATGCCATCCATTGGTACAGCTTCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCg                                                                                                                    <  1:1442936/140‑1 (MQ=255)                     tGCCAAGACGAATCAACAATACGGGCAAATAAGATGCCATCCATTGGTACAGCTTCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCg                                                                                                                    <  1:388672/138‑1 (MQ=255)                     tGCCAAGACGAATCAACAATACGGGCAAATAAGATGCCATCCATTGGTACAGCTTCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCg                                                                                                                    >  2:388672/1‑138 (MQ=255)                      gCCAAGACGAATCAACAATACGGGCAAATAAGATGCCATCCATTGGTACAGCTTCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGAAGCCCATCAGAGCGAAACCCCGGCa                                                                                                                 >  1:549794/1‑140 (MQ=255)                       ccAAGACGAATCAACAATACGGGCAAATAAGATGCCATCCATTGGTACAGCTTCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCAc                                                                                                                >  1:381633/1‑140 (MQ=255)                          aGACGAATCAACAATACGGGCAAATAAGATGCCATCCATTGGTACAGCTTCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTc                                                                                                             >  2:106147/1‑140 (MQ=255)                          aGACGAATCAACAATACGGGCAAATAAGATGCCATCCATTGGTACAGCTTCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTc                                                                                                             >  2:873796/1‑140 (MQ=255)                            aCGAATCAACAATACGGGCAAATAAGATGCCATCCATTGGTACAGCTTCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCaa                                                                                                           >  1:996879/1‑140 (MQ=255)                                aTCAACAATACGGGCAACTAAGATGCCATCCTTTGCTACAGCTTCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTg                                                                                                       <  2:549794/140‑1 (MQ=255)                                aTCAACAATACGGGCAAATAAGATGCCATCCATTGGTACAGCTTCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTg                                                                                                       >  1:1432735/1‑140 (MQ=255)                                aTCAACAATACGGGCAAATAAGATGCCATCCATTGGTACAGCTTCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTg                                                                                                       >  1:1481567/1‑140 (MQ=255)                                aTCAACAATACGGGCAAATAAGATGCCATCCATTGGTACAGCTTCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTg                                                                                                       >  2:1014238/1‑140 (MQ=255)                                 tCAACAATACGGGCAAATAAGATGCCATCCATTGGTACAGCTTCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTgg                                                                                                      >  1:146295/1‑139 (MQ=255)                                 tCAACAATACGGGCAAATAAGATGCCATCCATTGGTACAGCTTCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCAACAAAGCAAAACCCCGGCACTTCAACCTGc                                                                                                      >  1:519963/1‑140 (MQ=255)                                      aaTACGGGCAAATAAGATGCCATCCATTGGTACAGCTTCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTa                                                                                                 <  2:1432735/140‑1 (MQ=255)                                      aaTACGGGCAAATAACATGCCACCCATTGTTACAGCTACAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTa                                                                                                 <  1:523868/140‑1 (MQ=255)                                        tACGGGCAAATAAGATGCCATCCATTGGTACAGCTTCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGc                                                                                               >  2:1437735/1‑140 (MQ=255)                                         aCGGGCAAATAAGATGCCATCCATTGGTACAGCTTCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGcg                                                                                              <  2:1163059/140‑1 (MQ=255)                                          cGGGCAAATAAGATGCCATCCATTGGTACAGCTTCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGcgg                                                                                             >  2:217060/1‑140 (MQ=255)                                           gggCAAATAAGATGCCATCCATTGGTACAGCTTCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGcggt                                                                                            <  1:873796/140‑1 (MQ=255)                                           gggCAAATAAGATGCCATCCATTGGTACAGCTTCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGcggt                                                                                            <  1:1014238/140‑1 (MQ=255)                                           gggCAAATAAGATGCCATCCATTGGTACAGCTTCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGcggt                                                                                            <  1:1310410/140‑1 (MQ=255)                                           gggCAAATAAGATGCCATCCATTGGTACAGCTTCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGcggt                                                                                            <  2:5961/140‑1 (MQ=255)                                             gCAAATAAGATGCCATCCATTGGTACAGCTTCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAATCCCCGGCAGTTCACCCTGCGGGTAGcggtcg                                                                                          >  2:523868/1‑140 (MQ=255)                                             gCAAATAAGATGCCATCCATTGGTACAGCTTCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTa                                                                                                 <  1:364931/133‑1 (MQ=255)                                             gCAAATAAGATGCCATCCATTGGTACAGCTTCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTa                                                                                                 >  2:364931/1‑133 (MQ=255)                                              cAAATAAGATGCCATCCATTGGTACAGCTTCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTa                                                                                                 <  2:1171099/132‑1 (MQ=255)                                              cAAATAAGATGCCATCCATTGGTACAGCTTCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTa                                                                                                 >  1:1171099/1‑132 (MQ=255)                                              cAAATAAGATGCCATCCATTGGTACAGCTTCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGcggtcgg                                                                                         >  1:866130/1‑140 (MQ=255)                                                  tAAGATGCCATCCATTGGTACAGCTTCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGCGGTCGGtgat                                                                                     <  1:106147/140‑1 (MQ=255)                                                   aaGATGCCATCCATTGGTACAGCTTCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGCGGTCGGtgatg                                                                                    <  1:850603/140‑1 (MQ=255)                                                     gATGCCATCCATTGGTACAGCTTCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGCGGTCGGTGATGAg                                                                                  >  1:170271/1‑140 (MQ=255)                                                       tGCCATCCATTGGTACAGCTTCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGCGGTCGGTGATGAGCa                                                                                >  1:98020/1‑140 (MQ=255)                                                       tGCCATCCATTGGTACAGCTTCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGCGGTCGGTGATGAGCa                                                                                >  1:1128675/1‑140 (MQ=255)                                                         ccatccatTGGTACAGCTTCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGCGGTCGGTGATGAGCAGa                                                                              <  1:182902/140‑1 (MQ=255)                                                            tccatTGGTACAGCTTCAGCTCGCGCTGCCTTAACATTTTTATTCAACCGTTACGGCCCGTGGTGTTAGCCAATTGCAGGCCATCAAAGCGAAACGCGGGCACTTCAACCTGCGGGTAGCGGTCGGTGATGTGCAGaacg                                                                           >  2:1532271/1‑138 (MQ=255)                                                            tccatTGGTACAGCTTCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGCGGTCGGTGATGAGCAGAAAg                                                                           >  2:239310/1‑140 (MQ=255)                                                            tccatTGGTACAGCTTCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCAATTCAACCTGCGAGTAGCGGTCAGTGATGAGCAGAAAg                                                                           >  1:650006/1‑140 (MQ=255)                                                               atTGGTACAGCTTCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTAAACCTGCGGGTCGCAGTCGGTCATGAGCAGAAAGCCt                                                                        >  1:716997/1‑140 (MQ=255)                                                                ttGGTACAGCTTCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGCGGTCGGTGATGAGCAGAAAGCCtt                                                                       >  2:233164/1‑140 (MQ=255)                                                                   gTACAGCTTCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGCGGTCGGTGATGAGCAGAAAGCCTTGCt                                                                    <  2:381633/140‑1 (MQ=255)                                                                   gTACAGCTTCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGCGGTCGGTGATGAGCAGAAAGCCTTGCt                                                                    <  2:191223/140‑1 (MQ=255)                                                                   gTACAGCTTCAGCTCGAGCTGCTTTAACATTTTCAGCCAACCGTTACCCCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGCGGTCGGTGATGAGCAGAAAGCCTTGCt                                                                    <  2:716997/140‑1 (MQ=255)                                                                      cAGCTTCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGCGGTCGGTGATGAGCAGAAAGCCTTGCTGGc                                                                 <  1:877347/140‑1 (MQ=255)                                                                      cAGCTTCAGCTCGCGCATCCTTTACATTTTTGTTCTACCGTTACCGCCCGTGGTTTTTGCTAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGCGGTCGGTGATGAGCAGAAAGCCTTGCTGGc                                                                 <  2:672426/140‑1 (MQ=255)                                                                        gCTTCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGCGGTCGGTGATGAGCAGAAAGCCTTGCTGGCtt                                                               >  2:813590/1‑140 (MQ=255)                                                                        gCTTCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGCGGTCGGTGATGAGCAGAAAGCCTTGCTGGCtt                                                               <  1:217060/140‑1 (MQ=255)                                                                        gCTTCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGCGGTCGGTGATGAGCAGAAAGCCTTGCTGGCtt                                                               <  2:168592/140‑1 (MQ=255)                                                                        gCTTCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGATACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACCTCAACCTGCGGGGAGCCGTCGGTGAAGAGCGGAAAGCCTTGCTGGCtt                                                               >  2:658631/1‑140 (MQ=255)                                                                         cTTCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGCGGTCGGTGATGAGCAGAAAGCCTTGCTGGCttt                                                              >  1:935647/1‑140 (MQ=255)                                                                           tCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGCGGTCGGTGATGAGCAGAAAGCCTTGCTGGCTTTTa                                                            >  1:296029/1‑140 (MQ=255)                                                                             aGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGCGGTCGGTGATGAGCAGAAAGCCTTGCTGGCTTTTaca                                                          >  1:1154557/1‑140 (MQ=255)                                                                              gCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGCTGTCGGTGATGATCAGAAAGCCTTGCTGGCTTTTacac                                                         >  1:681751/1‑140 (MQ=255)                                                                              gCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGCGGTCGGTGATGAGCAGAAAGCCTTGCTGGCTTTTacac                                                         >  1:1093539/1‑140 (MQ=255)                                                                                   cgcTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGCGGTCGATGATGAGAAGAAAGACTTGCTGGCTTTTACACAACAt                                                    >  1:703207/1‑140 (MQ=255)                                                                                       gCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGCGGTCGGTGATGAGCAGAAAGCCTTGCTGGCTTTTACACAACATTTTg                                                <  2:1154557/140‑1 (MQ=255)                                                                                                  tttATCCAACCGTTCCCGCCCGCGGGTTTCGCCAATTCCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGCGGTCGGTGATGAGCAGAAAGCCTTGCTGGCTTTTACACAACATTTTGCGAAACTTGTa                                     <  1:511490/140‑1 (MQ=255)                                                                                                  tttATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGCGGTCGGTGATGAGCAGAAAGCCTTGCTGTCTTTTAAACAACATTTTGCGAAACTTGTa                                     >  1:683589/1‑140 (MQ=255)                                                                                                  tttATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGCGGTCGGTGATGAGCAGAAAGCCTTGCTGGCTTTTACACAACATTTTGCGAAACTTGTa                                     >  1:78740/1‑140 (MQ=255)                                                                                                  tttATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGCGGTCGGTGATGAGCAGAAAGCCTTGCTGGCTTTTACACAACATTTTGCGAAACTTGTa                                     >  1:853941/1‑140 (MQ=255)                                                                                                  tttATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGCGGTCGGTGATGAGCAGAAAGCCTTGCTGGCTTTTACACAACATTTTGCGAAACTTGTa                                     >  2:816274/1‑140 (MQ=255)                                                                                                    tATCCACCCGTTCCCGCCCGTGGTTTTTGCCAATTGCAGGCCATAAAAGCGAACCCCCGGCACTTCACCCTGCGGGTAGCGGTCGGTGATGAGCAGAAAGCCTTGCTGGCTTTTACACAACATTTTGCGAAACTTGTAcg                                   <  2:1128675/140‑1 (MQ=255)                                                                                                       ccAACCGTTCCCGCCCGGGGTTGTTGCCAATCGGAGGCCATCAAAGCGTACCCCCGGCACTTCAACCTGCGGGTAGCGGTCGGTGATGAGCAGAAAGCCTTGCTGGCTTTTACACAACATTTTGCGAAACTTGTAcgccc                                <  2:681751/140‑1 (MQ=255)                                                                                                           ccGTTACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGCGGTCGGTGATGAGCAGAAAGCCTTGCTGGCTTTTACACAACATTTTGCGAAACTTGTACGCCCGCca                            >  1:867719/1‑140 (MQ=255)                                                                                                              ttACCGCCCGTGGTTTTTGCCATTTCCAGTCCATCAAGGCGAAACCCCGGCACTTCAACCTGCGGGTAGCGGTCGGTGATGAGCAGAAAGCCTTGCTGGCTTTTACACAACATTTTGCGAAACTTGTACGCCCGCcagca                         <  1:518571/140‑1 (MQ=255)                                                                                                              ttACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAACCCCCGGCACTTCAACCTGCGGGTAGCGGTCGGTGATGAGCAGAAAGCCTTGCTGGCTTTTACACAACATTTTGCGAAACTTGTACGCCCGCcagca                         <  2:56110/140‑1 (MQ=255)                                                                                                               tACCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGCGGTCGGTGATGAGCAGAAAGCCTTGCTGGCTTTTACACAACATTTTGCGAAACTTGTACGCCCGCcagcag                        >  2:1342888/1‑140 (MQ=255)                                                                                                                aCCGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGCGGTCGGTGATGAGCAGAAAGCCTTGCTGGCTTTTACACAACATTTTGCGAAACTTGTACGCCCGCCAGCAgg                       >  1:59641/1‑140 (MQ=255)                                                                                                                 ccGCCCGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGCGGTCGGTGATGAGCAGAAAGCCTTGCTGGCTTTTACACAACATTTTGCGAAACTTGTACGCCCGCCAGCAGGa                      <  1:813590/140‑1 (MQ=255)                                                                                                                    cccGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGCGGTCGGTGATGAGCAGAAAGCCTTGCTGGCTTTTACACAACATTTTGCGATACATGTACGACCGCCAGCAGGAAAg                   >  1:729901/1‑140 (MQ=255)                                                                                                                      cGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCACCCTGCGGGTAGCGGCCGGTGATGAGCAGAAAGCCTTGCGGGTTTTTACACAACATTTTGCGAATTTTGTACGCCCGCCAGCAGGAAAGCa                 <  2:146295/140‑1 (MQ=255)                                                                                                                      cGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGCGGTCGGTGATGAGCAGAAAGCCTTGCTGGCTTTTACACAACATTTTGCGAAACTTGTACGCCCGCCAGCAGGAAAGCa                 <  2:1481567/140‑1 (MQ=255)                                                                                                                      cGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGCGGTCGGTGATGAGCAGAAAGCCTTGCTGGCTTTTACACAACATTTTGCGAAACTTGTACGCCCGCCAGCAGGAAAGCa                 <  2:170271/140‑1 (MQ=255)                                                                                                                      cGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGCGGTCGGTGATGAGCAGAAAGCCTTGCTGGCTTTTACACAACATTTTGCGAAACTTGTACGCCCGCCAGCAGGAAAGCa                 <  1:239310/140‑1 (MQ=255)                                                                                                                      cGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGCGGTCGGTGATGAGCAGAAAGCCTTGCTGGCTTTTACACAACATTTTGCGAAACTTGTACGCCCGCCAGCAGGAAAGCa                 <  1:950604/140‑1 (MQ=255)                                                                                                                      cGTGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGCGGTCGGTGATGAGCAGAAAGCCTTGATGGCTTTTACACAACATTTTGCGAAACTTGTACGCCCGCCAGCAGGAAAGCa                 <  1:1192080/140‑1 (MQ=255)                                                                                                                      cGTGGTTTTTGCCAACTGCAGGCCAGCAAGGCGAAACCCCGGCACTTCAACCTGCGGGTAGCGGTCGGTGATGAGCAGAAAGCCTTGCTGGCTTTTACACAACATTTTGCGAAACTTGTACGCCCGCCAGCAGGAAAGCa                 <  1:658631/140‑1 (MQ=255)                                                                                                                        tGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGCGGTCGGTGATGAGCAGAAAGCTTTGCTGGCTTTTACACAACATTTTGCGAAACTTGAACGCCCGCCAGCAGGAAAGCaga               <  1:1532271/140‑1 (MQ=255)                                                                                                                        tGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGCGGTCGGTGATGAGCAGAAAGCCTTGCTGGCTTTTACACAACATTTTGCGAAACTTGTACGCCCGCCAGCAGGAAAGCaga               >  2:375980/1‑140 (MQ=255)                                                                                                                        tGGTTTTTGCCAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGCGGTCGGTGATGAGCAGAAAGCCTTGCTGGCTTTTACACAACATTCTGCCAAACTTGTACGCCAGCCAGCAGGAAAGCaga               >  2:592957/1‑140 (MQ=255)                                                                                                                                 ccAATTGCAGGCCATCAAAGCAGAACCCCGGACATCCAAACTGCGGGTTGCGGTCGGTGAGGAGAAAAAAGCCTTGCCGGTTTTTACACAACATTTTGAAAAAATTGTCCGCCCGCCAGCAGGAAAGCAGATAGATAAcc      <  2:703207/140‑1 (MQ=255)                                                                                                                                 caaaTTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGCGGTCGGTGATGAGCAGAAAGCCTTGCTGGCTTTTACACAACATTTTGCGAAACTTGTACGCCCGCCAGCAGGAAAGCAGATAGATAAcc      <  1:233164/138‑1 (MQ=255)                                                                                                                                  cAATTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGCGGTCGGTGATGAGCAGAAAGCCTTGCTGGCTTTTACACAACATTTTGCGAAAATTGTACGCCCGACAGCAGGAATGCAGATAGATAACCa     >  1:495536/1‑140 (MQ=255)                                                                                                                                  cAATTGCAGGCCACCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGCGGTCGGTGATGAGCAGAAAGCCTTGCTGGCTTTTACACAACATTTTGCGAAACTTGTACGCCCGCCAGCAGGAAAGCAGATAGATAACCa     <  1:831222/140‑1 (MQ=255)                                                                                                                                   aaTTGCAGGCCATCAACCCGAATCCCCGGCAATTCAACCTGCGGGTAGCGGTCGGTGATGAGCAGAAAGCCTTGCTGGCTTTTACACAACATTTTGCGAAACTTGTACGCCCGCCAGCAGGAAAGCAGATAGATAACCAg    <  2:683589/140‑1 (MQ=255)                                                                                                                                   aaTTGCAGGCCATCAAAGCGAAACCCCGGCACTTCACCCTGCGGGTAGCGGTCGGTGATGAGCAGAAAGCCTTGCTGGCTTTTACACAACATTTTGCGAAACTTGTACGCCCGCCAGCAGGAAAGCAGATAGATAACCAg    <  2:1518083/140‑1 (MQ=255)                                                                                                                                   aaTTGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGCGGTCGGTGATGAGCAGAAAGCCTTGCTGGCTTTTACACAACATTTTGCGAAACTTGTACGCCCGCCAGCAGGAAAGCAGATAGATAACCAg    <  2:98020/140‑1 (MQ=255)                                                                                                                                     ttGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGCGGTCGGTGATGAGCAGAAAGCCTTGCTGGCTTTTACACAACATTTTGCGAAACTTGTACGCCCGCCAGCAGGAAAGCAGATAGATAACCAGTg  >  2:73416/1‑140 (MQ=255)                                                                                                                                     ttGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGCGGTCGGTGATGAGCAGAAAGCCTTGCTGGCTTTTACACAACATTTTGCGAAACTTGTACGCCCGCCAGCAGGAAAGCAGATAGATAACCAGTg  >  1:842766/1‑140 (MQ=255)                                                                                                                                     ttGCAGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGCGGTCGGTGATGAGCAGAAAGCCTTGCTGGCTTTTACACAACATTTTGCGAAACTTGTACGCCCGCCAGCAGGAAAGCAGATAGATAACCAGTg  <  1:816274/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        AAACGCGGTTTGTTCATCAATGCCAAGACGAATCAACAATACGGGCAAATAAGATGCCATCCATTGGTACAGCTTCAGCTCGCGCTGCCTTAACATTTTTATCCAACCGTTACCGCCCGTGGTTTTTGCCAATTGCGGGCCATCAAAGCGAAACCCCGGCACTTCAACCTGCGGGTAGCGGTCGGTGATGAGCAGAAAGCCTTGCTGGCTTTTACACAACATTTTGCGAAACTTGTACGCCCGCCAGCAGGAAAGCAGATAGATAACCAGTG  >  NC_000913/3133406‑3133677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑