BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,246,323	G→A	G367E (GGG→GAG)	exuT →	hexuronate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,246,323	0	G	A	98.5%	434.5 / NA	131	G367E (GGG→GAG)	exuT	hexuronate transporter
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (68/60); minor base C (0/2); total (68/63)
Fisher's exact test for biased strand distribution p-value = 2.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 133 total aligned reads displayed.

TTCACCAACCCGTATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTG  >  NC_000913/3246187‑3246456

ttCACCAACCCGTATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGAt                                                                                                                                    >  2:226342/1‑140 (MQ=255)

ttCACCAACCCGTATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGAt                                                                                                                                    <  1:1247996/140‑1 (MQ=255)

     cAACCCGTATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCg                                                                                                                               >  1:1035470/1‑140 (MQ=255)

     cAACCCGTATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCg                                                                                                                               <  2:378831/140‑1 (MQ=255)

          cGTATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCAtgg                                                                                                                          >  1:999308/1‑140 (MQ=255)

          cGTATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCAtgg                                                                                                                          >  2:1093815/1‑140 (MQ=255)

            tATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCAtggct                                                                                                                        >  2:1185211/1‑140 (MQ=255)

            tATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCAtggct                                                                                                                        <  2:1035470/140‑1 (MQ=255)

              tGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCAtggctgg                                                                                                                      <  1:1530056/140‑1 (MQ=255)

                  gCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAg                                                                                                                  <  2:62243/140‑1 (MQ=255)

                   cAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGt                                                                                                                 >  2:1202332/1‑140 (MQ=255)

                   cAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGt                                                                                                                 >  2:1287254/1‑140 (MQ=255)

                   cAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGt                                                                                                                 >  2:243998/1‑140 (MQ=255)

                    aaTTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTa                                                                                                                <  1:1502956/140‑1 (MQ=255)

                      ttATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACg                                                                                                              >  2:334167/1‑140 (MQ=255)

                         tgctgcTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTg                                                                                                           >  1:339471/1‑140 (MQ=255)

                           ctgctgTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGtt                                                                                                         >  1:1546143/1‑140 (MQ=255)

                           aggctgTGTATCGGTCGTTTTGCCCACCAAGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGtt                                                                                                         <  1:582922/138‑1 (MQ=255)

                             gctgTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTg                                                                                                       <  1:1060541/140‑1 (MQ=255)

                              ctgTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTgc                                                                                                      >  2:78360/1‑140 (MQ=255)

                              ctgTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTgc                                                                                                      >  1:1480185/1‑140 (MQ=255)

                                gtgtATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACTGAGATGTTCGCGTGGCTGGCATGTACGCTGTTTgcgc                                                                                                    >  1:746151/1‑140 (MQ=255)

                                gtgtATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTgcgc                                                                                                    >  1:954556/1‑140 (MQ=255)

                                        gtggtTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATTTCCTCATAGCTGGCAAATACGTTGTTTGCGCTGGTTgct                                                                                            >  2:582922/1‑138 (MQ=255)

                                        gtggtTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTc                                                                                            <  1:157140/140‑1 (MQ=255)

                                        gtggtTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTc                                                                                            >  1:187262/1‑140 (MQ=255)

                                           gtTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACGGAGATGTCGGCAAGGCTGGCAAGTAAGCTGGTTGCGCTGGTTGTCGGt                                                                                         >  2:461258/1‑140 (MQ=255)

                                           gtTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGt                                                                                         >  2:1240373/1‑140 (MQ=255)

                                           gtTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGt                                                                                         >  1:875198/1‑140 (MQ=255)

                                           gtTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCAGCAAGGCTGGCAAGTACGCTGGTTGCGCTGGTTGTCGGt                                                                                         >  2:422826/1‑140 (MQ=255)

                                            ttttGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTg                                                                                        <  2:67758/140‑1 (MQ=255)

                                                     ccaGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTg                                                                               >  1:177600/1‑140 (MQ=255)

                                                      caGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCa                                                                                      >  2:1547847/1‑132 (MQ=255)

                                                      caGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCa                                                                                      <  1:1547847/132‑1 (MQ=255)

                                                      caGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGa                                                                              <  1:758313/140‑1 (MQ=255)

                                                        ggCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGaca                                                                            <  2:339471/140‑1 (MQ=255)

                                                        ggCACTGTCTGGCGCGCTGATTACGCTCTCTACCGGTGTGTTTGGTCGGAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGaca                                                                            <  2:646305/140‑1 (MQ=255)

                                                         gCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTCGCGGTGGTTGTCGGTGCACTGGCTGacac                                                                           >  1:671946/1‑140 (MQ=255)

                                                           aCTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCa                                                                         >  1:790561/1‑140 (MQ=255)

                                                            cTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCAt                                                                        <  2:161898/140‑1 (MQ=255)

                                                                cTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGTGCTGGTTGTCGGTGCACTGGCTGACACCATCGGc                                                                    >  2:47032/1‑140 (MQ=255)

                                                                cTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTGTGCGCTGTTTGGCGGTGCACTGGCTGACACCATCGGc                                                                    >  1:404592/1‑140 (MQ=255)

                                                                 tGGCGCGCTGATTACGCTCTCTGCCTATGCGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCt                                                                   <  1:447220/140‑1 (MQ=255)

                                                                  ggCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCtt                                                                  >  1:143771/1‑140 (MQ=255)

                                                                   gcgcgcTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTc                                                                 >  2:1354498/1‑140 (MQ=255)

                                                                   gcgcgcTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTc                                                                 >  2:1465411/1‑140 (MQ=255)

                                                                    cgcgcTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCa                                                                >  1:935456/1‑140 (MQ=255)

                                                                    cgcgcTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCa                                                                >  1:1529674/1‑140 (MQ=255)

                                                                       gcTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGcc                                                             >  2:1148949/1‑140 (MQ=255)

                                                                        cTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGccc                                                            <  1:1029782/140‑1 (MQ=255)

                                                                                gCTCTCTTCCGATGTGTGTGGTTGTAACGAACTGGTAACCGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCg                                                    <  2:404592/140‑1 (MQ=255)

                                                                                 ctctctTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGc                                                   <  2:875198/140‑1 (MQ=255)

                                                                                 ctctctTCCGATGTGTTAGGTCCTACCGAAGAGTCGACGACAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGc                                                   <  2:704588/140‑1 (MQ=255)

                                                                                     ctTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGc                                                   <  1:1488731/136‑1 (MQ=255)

                                                                                     ctTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGtt                                               <  1:225585/140‑1 (MQ=255)

                                                                                     ctTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGtt                                               <  1:1185211/140‑1 (MQ=255)

                                                                                     ctTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGtt                                               >  1:1045999/1‑140 (MQ=255)

                                                                                     ctTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCAGCTTCAGCCCGCTGTTCGc                                                   >  2:1488731/1‑136 (MQ=255)

                                                                                     ctTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTGGTCGGTGCACTGGCTGACACCATCGGCTTCCGCCCGCTGTTCGCAGtt                                               >  2:524430/1‑140 (MQ=255)

                                                                                      ttCCGATGTGTTTGGTCCTAACTAAGTGGCACCGGCAAATGGTTTAACTGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTc                                              <  2:393946/140‑1 (MQ=255)

                                                                                       tCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCt                                             >  1:1414400/1‑140 (MQ=255)

                                                                                         cGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTgg                                           <  1:1093815/140‑1 (MQ=255)

                                                                                          gATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGc                                          <  2:187262/140‑1 (MQ=255)

                                                                                          gATGTGTTTGGTCGTAACGAAGCGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCACGCTGTTCGCAGTTCTGGc                                          <  1:524430/140‑1 (MQ=255)

                                                                                            tgtgTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAg                                        >  2:1398122/1‑140 (MQ=255)

                                                                                              tgtTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAgtg                                      >  2:64140/1‑140 (MQ=255)

                                                                                              tgtTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAgtg                                      >  2:961351/1‑140 (MQ=255)

                                                                                              tgtTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAgtg                                      >  2:43195/1‑140 (MQ=255)

                                                                                              tgtTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAgtg                                      >  2:1322079/1‑140 (MQ=255)

                                                                                                tttGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGtt                                    >  2:342644/1‑140 (MQ=255)

                                                                                                tttGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTAAGCCCGCTGTTCGTAGTTCTGGCAGTGtt                                    >  1:532090/1‑140 (MQ=255)

                                                                                                tttGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACAACATGGGCGTCAGCCCGGTGTTGGCAGTTCTGGCAGTGtt                                    >  2:408084/1‑140 (MQ=255)

                                                                                                tttGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACAACATCGGCATCAGCCCGATGTTCGCAGTTCTGGCAGTGtt                                    >  2:586378/1‑140 (MQ=255)

                                                                                                 ttGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGGTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTc                                   <  2:935456/140‑1 (MQ=255)

                                                                                                 ttGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTc                                   <  1:64140/140‑1 (MQ=255)

                                                                                                  aggTCGTAACGAAGTGGCAACGCCATACGTTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCg                                  <  1:622298/139‑1 (MQ=255)

                                                                                                   ggTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTgg                                           >  2:1539155/1‑130 (MQ=255)

                                                                                                     tCGTAACGAAGTGGCAACGGCAAACGGTTTATCCGCGATGTCCGCATGACTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGAcc                               <  2:458019/140‑1 (MQ=255)

                                                                                                     tCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGAcc                               >  2:290219/1‑140 (MQ=255)

                                                                                                           cGAAGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGTGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAg                         <  1:47032/140‑1 (MQ=255)

                                                                                                             aaGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGt                       <  2:1529674/140‑1 (MQ=255)

                                                                                                              aGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTg                      <  2:1546143/140‑1 (MQ=255)

                                                                                                              aGTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTg                      <  1:913579/140‑1 (MQ=255)

                                                                                                               gTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTgc                     <  1:961351/140‑1 (MQ=255)

                                                                                                               gTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTgc                     <  2:143771/140‑1 (MQ=255)

                                                                                                               gTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTgc                     >  2:1481078/1‑140 (MQ=255)

                                                                                                               gTGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTgc                     <  1:342644/140‑1 (MQ=255)

                                                                                                                tGGCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTgcg                    <  1:1398122/140‑1 (MQ=255)

                                                                                                                 ggCAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGTAGTGTTCGACCTGTTAGGTgcgc                   <  2:177600/140‑1 (MQ=255)

                                                                                                                   cAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTg                 >  1:1532807/1‑140 (MQ=255)

                                                                                                                   cAACGGCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTg                 <  2:912973/140‑1 (MQ=255)

                                                                                                                       ggCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTa             >  2:1037787/1‑140 (MQ=255)

                                                                                                                        gCAAACGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTAt            <  1:243998/140‑1 (MQ=255)

                                                                                                                           aaCGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTg         >  2:1141907/1‑140 (MQ=255)

                                                                                                                           aaCGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTg         <  1:43195/140‑1 (MQ=255)

                                                                                                                             cGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGa       <  1:1148949/140‑1 (MQ=255)

                                                                                                                             cGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGa       <  2:954556/140‑1 (MQ=255)

                                                                                                                             cGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGa       <  1:1247714/140‑1 (MQ=255)

                                                                                                                             cGGTTTAACCGAGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGa       <  1:1322079/140‑1 (MQ=255)

                                                                                                                             cGGTTTAACCGAGATGTACGCATGGCTGGCATGGATGCTTTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGa       <  2:671946/140‑1 (MQ=255)

TTCACCAACCCGTATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCGCATGGCTGGCAAGTACGCTGTTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTTCTGGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTG  >  NC_000913/3246187‑3246456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`