BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,268,018	A→G	E55E (GAA→GAG)	yhaB →	protein YhaB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,268,018	0	A	G	99.4%	551.4 / NA	153	E55E (GAA→GAG)	yhaB	protein YhaB
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (72/80); minor base T (0/1); total (72/81)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 154 total aligned reads displayed.

TTTCACCCTTCAATCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAATATGAGTCCATTCATTATAGATATTTCTACAGGAGAACATGATGATTATA  >  NC_000913/3267881‑3268154

tttCACCCTTCAATCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCt                                                                                                                                        >  1:1022151/1‑140 (MQ=255)

    ccccTTCAATCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCgg                                                                                                                                    <  1:763992/139‑1 (MQ=255)

     cccTTCAATCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGga                                                                                                                                   <  1:137042/140‑1 (MQ=255)

         tCAATCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGGTAAGAAAGTTTACAGTGTAAGCAATGAGCTGCGGAGAGt                                                                                                                               >  2:461599/1‑140 (MQ=255)

         tCAATCACGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCgg                                                                                                                                    >  2:1504701/1‑135 (MQ=255)

             tCCCGCCAATGCACGCAGTGGTCAACAATCATAATTGAAATATTGATTCTTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGtatta                                                                                                                           <  2:581316/140‑1 (MQ=255)

              cccGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGtattat                                                                                                                          <  2:1004078/140‑1 (MQ=255)

               ccGCCAATGCACGCAGAGGTTAACAATCACAATAGCAATTTTGATTATGGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATc                                                                                                                         <  2:712130/140‑1 (MQ=255)

                cGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCt                                                                                                                        <  1:344400/140‑1 (MQ=255)

                  ccAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGc                                                                                                                      >  1:851570/1‑140 (MQ=255)

                  ccAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAAATTACAGTATAAGTAATAAGCTGCGGAGAGTATTATCTGc                                                                                                                      >  2:641328/1‑140 (MQ=255)

                  ccAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGAAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGAATAAGTAATGAGCTGCGGAGAGAATTATCGGc                                                                                                                      >  2:1162025/1‑140 (MQ=255)

                            cAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCa                                                                                                            <  2:1453342/140‑1 (MQ=255)

                                 gTTAACAATCACAATAGAAATATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATTAGCTGCGAAGAATAGTATCTGTAATAACTGCATTGaa                                                                                                       >  1:447673/1‑140 (MQ=255)

                                       aaTCACAATAGAAATATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTa                                                                                                 >  2:224455/1‑140 (MQ=255)

                                         tCACAATAGAAATATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATc                                                                                               >  2:1117420/1‑140 (MQ=255)

                                         tCACAATAGAAATATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATc                                                                                               >  2:846541/1‑140 (MQ=255)

                                         tCACAATAGAAATATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATc                                                                                               >  1:987111/1‑140 (MQ=255)

                                         tCACAATAGAAATATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATc                                                                                               >  1:1142669/1‑140 (MQ=255)

                                         tCACAATAGAAATATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATc                                                                                               >  1:1190834/1‑140 (MQ=255)

                                         tCACAATAGAAATATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATc                                                                                               >  1:105041/1‑140 (MQ=255)

                                           acaATAGAAATATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCAt                                                                                             >  1:1247381/1‑140 (MQ=255)

                                           acaATAGAAATATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCAt                                                                                             >  1:822975/1‑140 (MQ=255)

                                           acaATAGAAATATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCAt                                                                                             >  1:383210/1‑140 (MQ=255)

                                              aTAGAAATATTGATTGTTGGACGGTAAAACGAAAGTTCGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGc                                                                                          <  1:464348/140‑1 (MQ=255)

                                                aGAAATATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGa                                                                                        <  1:1354765/140‑1 (MQ=255)

                                                aGAAATATTGATTATTGCACGGGAAAAAGAAGGTTCGCAGAAATTGTCCCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGa                                                                                        <  2:447673/140‑1 (MQ=255)

                                                  aaaTATTGATTATTGTACGGTACAATGAACGTTTGCATAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATg                                                                                      <  1:419117/140‑1 (MQ=255)

                                                       ttGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCt                                                                                 >  1:1313454/1‑140 (MQ=255)

                                                        tGATTATTGGACGGTAACAAGACAGTTTGCACAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCtt                                                                                <  2:402777/140‑1 (MQ=255)

                                                        tGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCtt                                                                                <  2:1313454/140‑1 (MQ=255)

                                                        tGATTATTGGACGGTAAAAAGAAAGCTTGCAGAAATTCTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCtt                                                                                <  1:530067/140‑1 (MQ=255)

                                                            tattGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGt                                                                            >  1:1398192/1‑140 (MQ=255)

                                                            tattGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGt                                                                            >  1:825094/1‑140 (MQ=255)

                                                             attGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAAGTGCATTGGATTGCTGTCATGGGGATGTTCCTTCTGTc                                                                           >  1:402777/1‑140 (MQ=255)

                                                             attGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTc                                                                           <  1:799617/140‑1 (MQ=255)

                                                              ttGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCa                                                                          >  2:972487/1‑140 (MQ=255)

                                                              ttGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACAGCATTGACTTTCTATCATGGCTATGTTCCTTCTGTCa                                                                          >  1:606628/1‑140 (MQ=255)

                                                              ttGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTAACTGCAATAACTGAAGTGAATTTCTAGCATGTGGATGTTCGTTCTGTCa                                                                          >  2:751242/1‑140 (MQ=255)

                                                                 gACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATTTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGa                                                                       >  2:858426/1‑140 (MQ=255)

                                                                    ggTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATcc                                                                    >  1:1096150/1‑140 (MQ=255)

                                                                    ggTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATcc                                                                    >  1:1034418/1‑140 (MQ=255)

                                                                    ggTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATcc                                                                    >  2:160715/1‑140 (MQ=255)

                                                                      tAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTTTCATGGCGATGTTCCTTCTGTCATGATCCGa                                                                  >  2:550526/1‑140 (MQ=255)

                                                                      tAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGa                                                                  >  1:170141/1‑140 (MQ=255)

                                                                      tAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGa                                                                  >  2:274273/1‑140 (MQ=255)

                                                                      tAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGa                                                                  >  2:889521/1‑140 (MQ=255)

                                                                       aaaaaGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGaa                                                                 <  2:825094/140‑1 (MQ=255)

                                                                               agTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCg                                                         >  2:1415357/1‑140 (MQ=255)

                                                                                gTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAAc                                                           <  2:1354018/137‑1 (MQ=255)

                                                                                gTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAAc                                                           >  1:1354018/1‑137 (MQ=255)

                                                                                gTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCgg                                                        >  2:103338/1‑140 (MQ=255)

                                                                                gTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCAGGGCGATGTTCCATCAGTCATGGTCCGAATCCAACCgg                                                        >  1:513083/1‑140 (MQ=255)

                                                                                 tttGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGa                                                       <  2:1486729/140‑1 (MQ=255)

                                                                                 tttGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGa                                                       <  2:1096150/140‑1 (MQ=255)

                                                                                       gAAATTGTCTCCACCAAAGACGGTAATAAAAATTAAAGTATAAGTAATCAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAatat                                                 <  2:513083/140‑1 (MQ=255)

                                                                                           ttGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAATATg                                                <  1:847622/137‑1 (MQ=255)

                                                                                              tctcCACCAATGACGTTAATAAGATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAATATGAGTCca                                          <  1:602679/140‑1 (MQ=255)

                                                                                               ctcCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGa                                                       <  2:1543393/126‑1 (MQ=255)

                                                                                               ctcCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGa                                                       >  1:1543393/1‑126 (MQ=255)

                                                                                               ctcCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAATATGAGTCcat                                         <  1:160715/140‑1 (MQ=255)

                                                                                                tcCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAATATGAGTCcatt                                        <  2:1407627/140‑1 (MQ=255)

                                                                                                tcCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAATATGAGTCcatt                                        <  2:809918/140‑1 (MQ=255)

                                                                                                  caccaATGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAATATGAGTCcattca                                      <  2:851570/140‑1 (MQ=255)

                                                                                                      aaTGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCAT‑GCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAATATGAGTCCATTCATtata                                 <  1:885519/140‑1 (MQ=255)

                                                                                                       atgaacttAATAAAAGTTACAGCATCAGTCATGAGCTTCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAATATGAGTCCATTCATtata                                 <  1:722742/134‑1 (MQ=255)

                                                                                                       aTGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAATATGAGTCCATTCATtata                                 >  2:356929/1‑140 (MQ=255)

                                                                                                       aTGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAATATGAGTCCATTCATtata                                 <  1:192556/140‑1 (MQ=255)

                                                                                                       aTGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAATATGAGTCCATTCATtata                                 <  1:100814/140‑1 (MQ=255)

                                                                                                       aTGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAATATGAGTCCATTCATtata                                 <  2:874767/140‑1 (MQ=255)

                                                                                                       aTGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAATATGAGTCCATTCATtata                                 <  1:1111673/140‑1 (MQ=255)

                                                                                                       aTGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAAACCAACCGGGAAATATGAGTCCATTCATtata                                 >  2:731687/1‑140 (MQ=255)

                                                                                                        tGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAata                                                  >  1:175650/1‑122 (MQ=255)

                                                                                                        tGACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAata                                                  <  2:175650/122‑1 (MQ=255)

                                                                                                         gACGTTAATAAAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAATATGAGTCCATTCATTATAGa                               <  2:1022151/140‑1 (MQ=255)

                                                                                                          aCGTTAATAAAATTTACCGTATTAGTAATGATCTGTGGACAGTATTATGTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAATATGAGTCCATTCATTATAGat                              <  2:453533/140‑1 (MQ=255)

                                                                                                              taataaAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAATATGAGTCCATTCATTATAGATAttt                          >  2:248192/1‑140 (MQ=255)

                                                                                                               aataaAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCGAACCGGATAATGTGGGTCCATGCATTATAGATATTTc                         >  1:458087/1‑140 (MQ=255)

                                                                                                               aataaAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGACAATATGAGTCCATACATTATAGATATTTc                         >  1:632758/1‑140 (MQ=255)

                                                                                                               aataaAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAATATGAGTCCATTCATta                                   >  1:1259584/1‑130 (MQ=255)

                                                                                                               aataaAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAATATGAGTCCATTCATta                                   <  2:1259584/130‑1 (MQ=255)

                                                                                                               aataaAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAATATGAGTCCATTCATTATAGatat                            >  2:1279314/1‑137 (MQ=255)

                                                                                                               aataaAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAATATGAGTCCATTCATTATAGATATTTc                         >  2:1094128/1‑140 (MQ=255)

                                                                                                               aataaAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAATATGAGTCCATTCATTATAGATATTTc                         >  1:122725/1‑140 (MQ=255)

                                                                                                               aataaAATTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAATATGAGTCCATTCATTATAGATATTTc                         >  1:1427695/1‑140 (MQ=255)

                                                                                                                    aaTTTACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAATATGAGTCCATTCATTATAGATATTTCTACAg                    <  2:383210/140‑1 (MQ=255)

                                                                                                                      agacaggTATAAGTAATGAGCTGCGGAGAATATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAATATGAGTCCATTCATTATAGATATTTCTACAGga                  <  1:664589/134‑1 (MQ=255)

                                                                                                                       ttACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAATATGAGTCCATTCATTATAGATATTTCTACAGgag                 <  1:237822/140‑1 (MQ=255)

                                                                                                                       ttACAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAATATGAGTCCATTCATTATAGATATTTCTACAGgag                 <  2:190577/140‑1 (MQ=255)

                                                                                                                         atactgtaAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAATATGAGTCCATTCATTATAGATATTTCTACAGGAGaa               >  1:198331/7‑140 (MQ=255)

                                                                                                                          cAGTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAATATGAGTCCATTCATTATAGATATTTCTACAGGAGAAc              >  1:1441526/1‑140 (MQ=255)

                                                                                                                            gTATAAGTAATGAGCTGCGGAGTGTCTTACCTGCAATCACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAATATGAGTCCATTCATTATAGATATTTCTACAGGAGAACat            <  1:446671/140‑1 (MQ=255)

                                                                                                                            gTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAATATGAGTCCATTCATTATAGATATTTCTACAGGAGAACat            <  2:1247381/140‑1 (MQ=255)

                                                                                                                            gTATAAGTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAATATGAGTCCATTCATTATAGATATTTCTACAGGAGAACat            <  2:1441526/140‑1 (MQ=255)

                                                                                                                                 aGTAATGAGCTGCGGAGAGTATGATCTGCCATAACTGGATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAATATGAGTCCATTCATTATAGATATTTCTACAGGAGAACAtgatga       <  1:551603/140‑1 (MQ=255)

                                                                                                                                  gTAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAATATGAGTCCATTCATTATAGATATTTCTACAGGAGAACATgatgat      >  1:51745/1‑140 (MQ=255)

                                                                                                                                   tAATGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAGCCGGAAAATATGAGTCCATTCATTATAGATATTTCTACAGGAGAACATGATGAtt     <  2:1142669/140‑1 (MQ=255)

                                                                                                                                   tAATGAGCTGCGGAGAGCATTAACTGCAATATCTGCATTCAATTTCTACCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAATATGAGTCCATTCATTATAGATATTTCTACAGGAGAACATGATGAtt     <  2:411433/140‑1 (MQ=255)

                                                                                                                                     aTGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAATATGAGTCCATTCATTATAGATATTTCTACAGGAGAACATGATGATtat   <  2:122725/140‑1 (MQ=255)

                                                                                                                                      tGAGCTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAATATGAGTCCATTCATTATAGATATTTCTACAGGAGAACATGATGATtata  <  1:1157929/140‑1 (MQ=255)

TTTCACCCTTCAATCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATGATCCGAATCCAACCGGAAAATATGAGTCCATTCATTATAGATATTTCTACAGGAGAACATGATGATTATA  >  NC_000913/3267881‑3268154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`