Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,311,807	G→A	intergenic (‑123/+26)	rpsO ← / ← truB	30S ribosomal subunit protein S15/tRNA pseudouridine(55) synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,311,807	0	G	A	100.0%	331.5 / NA	97	intergenic (‑123/+26)	rpsO/truB	30S ribosomal subunit protein S15/tRNA pseudouridine(55) synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (62/35);  total (62/35)

AGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCC‑‑ATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTCC  >  NC_000913/3311670‑3311943                                                                                                                                          |                                                                                                                                           aGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCCATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAAc                                                                                                                                          <  2:1453414/140‑1 (MQ=255)     cTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGGTCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGt                                                                                                                                      <  2:1071306/140‑1 (MQ=255)     cTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGt                                                                                                                                      <  1:223796/140‑1 (MQ=255)       tAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTa                                                                                                                                    >  2:334294/1‑140 (MQ=255)            aCATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCa                                                                                                                               >  2:16523/1‑140 (MQ=255)             cATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTGCGCGACGTGAAGGGGCGGTATTCTATTCGTGGCAACCTGTTATCGCaa                                                                                                                              >  2:405962/1‑140 (MQ=255)             cATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTATGCGGCAGGATTCTACTCGTAGCAACCTGTTATCGCaa                                                                                                                              >  1:601240/1‑140 (MQ=255)             cATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCaa                                                                                                                              >  2:192986/1‑140 (MQ=255)              aTTTTAAAGCTCCAAAATATATCGAATGAAGCGACTCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCCACTCGTAGCAACCTGTTATCGCAAg                                                                                                                             <  2:723984/140‑1 (MQ=255)              aTTTTAAAACTCCAAAGTATATAGACTGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAg                                                                                                                             <  1:613151/140‑1 (MQ=255)              aTTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAg                                                                                                                             <  1:334294/140‑1 (MQ=255)                        tCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATGCTACTCGAAGCAACCTGTTATCGCAAGACGGTTAACa                                                                                                                   >  1:628873/1‑140 (MQ=255)                          cAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACAtt                                                                                                                 >  2:227917/1‑140 (MQ=255)                          cAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACAtt                                                                                                                 >  1:1034052/1‑140 (MQ=255)                          cAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACAtt                                                                                                                 >  2:1341948/1‑140 (MQ=255)                            aaGTATATAGAATGAAAGGACGACGATCTCTAATTTAGCAATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTAc                                                                                                               <  2:628873/140‑1 (MQ=255)                                 tataGAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCgg                                                                                                          <  1:192986/140‑1 (MQ=255)                                  ataGAATGAAAGGACTCCGATCGCTCATTTAGCGATCACAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCggg                                                                                                         <  1:728208/140‑1 (MQ=255)                                  ataGAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCggg                                                                                                         >  1:995171/1‑140 (MQ=255)                                    aGAATGAAAGGACGCAGATCTCCAATTCAACGATCCCTGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTa                                                                                                       <  1:697921/140‑1 (MQ=255)                                      aaTGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTTCTCGTAGCAACCTGGTGTCGCAAGACGGTTGACATTACGCCGGGTAtt                                                                                                     >  2:697921/1‑140 (MQ=255)                                      aaTGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTAtt                                                                                                     >  2:1362684/1‑140 (MQ=255)                                         gAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCGAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCaa                                                                                                  >  2:967546/1‑140 (MQ=255)                                         gAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCggg                                                                                                         >  1:911095/1‑133 (MQ=255)                                         gAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCggg                                                                                                         <  2:911095/133‑1 (MQ=255)                                         gAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCan                                                                                                  >  2:1085557/1‑139 (MQ=255)                                         gAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCaa                                                                                                  >  1:984411/1‑140 (MQ=255)                                         gAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCaa                                                                                                  >  2:238615/1‑140 (MQ=255)                                         gAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCaa                                                                                                  >  2:311206/1‑140 (MQ=255)                                         gAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCaa                                                                                                  >  1:353975/1‑140 (MQ=255)                                          aaaGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAac                                                                                                 <  2:984411/140‑1 (MQ=255)                                          aaaGGACGACGATCTCCAATTCAGCGATCCCAGTGGACGCTACGCAAACTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAac                                                                                                 <  2:724020/140‑1 (MQ=255)                                           aaGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAacc                                                                                                >  2:938592/1‑140 (MQ=255)                                           aaGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTCATCGCAATACGCTTGACATTATGCCGGGTATTCAacc                                                                                                >  1:724020/1‑140 (MQ=255)                                                cGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCa                                                                                                   >  1:1248820/1‑132 (MQ=255)                                                cGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCa                                                                                                   <  2:1248820/132‑1 (MQ=255)                                                cGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAg                                                                                           >  2:322149/1‑140 (MQ=255)                                                cGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAg                                                                                           >  1:1030429/1‑140 (MQ=255)                                                cGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAg                                                                                           >  2:1444835/1‑140 (MQ=255)                                                cGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAg                                                                                           >  2:1138913/1‑140 (MQ=255)                                                cGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAg                                                                                           >  2:1393411/1‑140 (MQ=255)                                                  ccGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGc                                                                                         >  1:33647/1‑140 (MQ=255)                                                    gATCTCTAATTCAGCAATCCCAATGCACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGATAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGcga                                                                                       <  1:638894/140‑1 (MQ=255)                                                     aTCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGAGGGTTAACATTACGCTGGGTATTCAACCACCAGGcgac                                                                                      >  1:575360/1‑140 (MQ=255)                                                           aaTTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGgcg                                                                                <  1:322149/140‑1 (MQ=255)                                                                  cGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAAcgcg                                                                         >  1:38735/1‑140 (MQ=255)                                                                    aTCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGc                                                                       >  2:1402340/1‑140 (MQ=255)                                                                    aTCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATT‑AACCACCAGGCGACGAGGCGCAACGCGGcc                                                                      >  2:941575/1‑140 (MQ=255)                                                                      cccAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGTGTATTCAGCCAGCACGCGACGCGGCGCAACGCGGCCt                                                                     >  1:468515/1‑140 (MQ=255)                                                                       ccAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCtt                                                                    <  2:38735/140‑1 (MQ=255)                                                                           tgtACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATc                                                                >  1:1474622/1‑140 (MQ=255)                                                                             tACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGt                                                              <  2:1474622/140‑1 (MQ=255)                                                                               cGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCa                                                            >  2:1499815/1‑140 (MQ=255)                                                                                 ttACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAAt                                                          <  1:1362684/140‑1 (MQ=255)                                                                                   aCGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAAttt                                                        >  1:611592/1‑140 (MQ=255)                                                                                    cGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTc                                                       >  2:793411/1‑140 (MQ=255)                                                                                    cGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGAACCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGAATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTc                                                       >  1:1324027/1‑140 (MQ=255)                                                                                     gCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCg                                                      >  1:1293926/1‑140 (MQ=255)                                                                                     gCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAAGCGACGATGCGTAACGCGGCCGTCATCGTCAATTTCg                                                      >  1:693049/1‑140 (MQ=255)                                                                                     gCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGCTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCg                                                      >  1:125754/1‑140 (MQ=255)                                                                                         ggtgtTAAACAATTTACCCGACCTTAAGCGGCAGTTTTCCACTCGTAGTAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCC‑‑a                                                <  2:472600/139‑1 (MQ=255)                                                                                         aGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCC‑‑a                                                >  2:1449926/1‑140 (MQ=255)                                                                                            gtTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCC‑‑ATAc                                             >  2:1537777/1‑140 (MQ=255)                                                                                            gtTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCC‑‑ATAc                                             >  2:350023/1‑140 (MQ=255)                                                                                              tAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCC‑‑ATACCg                                           >  2:1003162/1‑140 (MQ=255)                                                                                                  cAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCC‑‑ATACCGATaa                                       <  1:311206/140‑1 (MQ=255)                                                                                                     tttACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCC‑‑ATACCGATAAAtt                                    >  2:76799/1‑140 (MQ=255)                                                                                                     tttACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCC‑‑ATACCGATAAAtt                                    >  2:192283/1‑140 (MQ=255)                                                                                                     tttACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCC‑‑ATACCGATAAAtt                                    >  2:262202/1‑140 (MQ=255)                                                                                                     tttACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCC‑‑ATACCGATAAAtt                                    >  2:1396839/1‑140 (MQ=255)                                                                                                     tttACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTAATCGTCGATTTCGTCC‑‑ATACCGATAAAtc                                    >  1:472600/1‑139 (MQ=255)                                                                                                     tttACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTAATCGTCAATTTCGTCC‑‑ATACCGATAAAtt                                    >  1:663846/1‑140 (MQ=255)                                                                                                       tACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCC‑‑ATACCGATGAATTTg                                  <  1:1537777/140‑1 (MQ=255)                                                                                                       tACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCC‑‑ATACCGATAAATTTg                                  <  2:1163603/140‑1 (MQ=255)                                                                                                       tACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCC‑‑ATACCGATAAATTTg                                  <  1:350023/140‑1 (MQ=255)                                                                                                       tACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCC‑‑ATACCGATAAATTTg                                  >  1:1163603/1‑140 (MQ=255)                                                                                                       tACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCC‑‑ATACCGATAAATTTg                                  <  2:1030429/140‑1 (MQ=255)                                                                                                           cgACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCC‑‑ATACCGATAAATTTGCCGt                              <  1:1003162/140‑1 (MQ=255)                                                                                                            gACGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCC‑‑ATACCGATAAATTTGCCGtt                             <  1:938592/140‑1 (MQ=255)                                                                                                             aCGTTAAGCGGCAGTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCC‑‑ATACCGATAAATTTGCCGTtc                            >  1:197930/1‑140 (MQ=255)                                                                                                                          gTATTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATCACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCC‑‑ATACAGATAAAATTGCCGTTCTCACCTTCCGTGa               >  1:534998/1‑140 (MQ=255)                                                                                                                            aTTCTACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCC‑‑ATACCGATAAATTTGCCGTTCTCACCTTCCGTGAcg             <  2:33647/140‑1 (MQ=255)                                                                                                                                tACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCC‑‑ATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGaa         <  1:1444835/140‑1 (MQ=255)                                                                                                                                tACTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCC‑‑ATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGaa         <  1:129884/140‑1 (MQ=255)                                                                                                                                  cTCGTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCC‑‑ATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAAcc       >  1:1308556/1‑140 (MQ=255)                                                                                                                                     gTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCC‑‑ATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCa      >  1:1030136/1‑138 (MQ=255)                                                                                                                                     gTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCC‑‑ATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCa      <  2:1030136/138‑1 (MQ=255)                                                                                                                                     gTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCC‑‑ATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGt    >  1:1147067/1‑140 (MQ=255)                                                                                                                                     gTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCC‑‑ATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGt    <  2:1308556/140‑1 (MQ=255)                                                                                                                                     gTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCC‑‑ATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGt    >  1:870557/1‑140 (MQ=255)                                                                                                                                     gTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCC‑‑ATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGt    <  1:227917/140‑1 (MQ=255)                                                                                                                                     gTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCC‑‑ATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGt    <  1:1341948/140‑1 (MQ=255)                                                                                                                                     gTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCC‑‑ATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGt    >  1:1256395/1‑140 (MQ=255)                                                                                                                                     gTAGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGACCTTCATCGTCAATTTCGCCC‑‑ATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGt    >  1:1258830/1‑140 (MQ=255)                                                                                                                                       aGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCCATATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGt    <  1:1087279/140‑1 (MQ=255)                                                                                                                                       aGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCCATATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGt    >  2:1087279/1‑140 (MQ=255)                                                                                                                                       aGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCC‑‑ATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTcc  >  1:285023/1‑140 (MQ=255)                                                                                                                                       aGCAACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATGTCGCGC‑‑ATACCGACAAACTTGACGTTCTCAACTTCCGTGACGCGAACCAGTcc  >  2:466261/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                           AGTACTTAGAGACATTTTAAAACTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGACGGTTAACATTACGCCGGGTATTCAACCACCAGGCGACGAGGCGCAACGCGGCCTTCATCGTCAATTTCGCCC‑‑ATACCGATAAATTTGCCGTTCTCACCTTCCGTGACGCGAACCAGTCC  >  NC_000913/3311670‑3311943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑