Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,331,902	C→T	M278I (ATG→ATA)	yhbE ←	inner membrane protein YhbE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,331,902	0	C	T	100.0%	278.7 / NA	81	M278I (ATG→ATA)	yhbE	inner membrane protein YhbE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (41/40);  total (41/40)

GTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTTACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCAT  >  NC_000913/3331765‑3332038                                                                                                                                          |                                                                                                                                         gTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTTACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAatat                                                                                                                                        >  2:368640/1‑140 (MQ=255)     aTCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTTACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGt                                                                                                                                    <  1:352114/140‑1 (MQ=255)     aTCATTCGCCTGCGCGGCGTTGAGATACCACCGTTATATCCTTACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGt                                                                                                                                    <  2:621985/140‑1 (MQ=255)       cATTCGCCAGCGCGGGCTTGCGATACCACCCTTGTATGCATACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCt                                                                                                                                  <  2:738429/140‑1 (MQ=255)           cGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTTACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCg                                                                                                                              <  1:133935/140‑1 (MQ=255)               tGCGCGGGGTTGCGATACCACCGTTGTATGCTTACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAATAGCTTTAATGTCGGTCTGGCgaaga                                                                                                                          >  1:725392/1‑140 (MQ=255)               tGCGCGGGGTTGCGATACCACCGTTGTATGCTTACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCgaaga                                                                                                                          >  1:913094/1‑140 (MQ=255)                gcgcGGGGTTGCGATACCACCGTTGTATGCTTACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCgaagaa                                                                                                                         <  2:989360/140‑1 (MQ=255)                  gcgGGGTTGCGATACTACCGTTTTACGCTTACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAAt                                                                                                                       <  1:590234/140‑1 (MQ=255)                  gcgGGGTTGCGATACCACCGTTGTATGCTTACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAAt                                                                                                                       <  2:874830/140‑1 (MQ=255)                  gcgGGGTTGCGATACCACCGTTGTATGCTTACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAAt                                                                                                                       <  2:134024/140‑1 (MQ=255)                  gcgGGGTTGCGATACCACCGTTGTATGCTTACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAAt                                                                                                                       <  1:368640/140‑1 (MQ=255)                  gcgGGGTTGCGATACCACCGTTGTATGCTTACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAAt                                                                                                                       <  2:1503583/140‑1 (MQ=255)                           cGATACCACCGTTGTATGCTTACGTTATCTGCCCCAAGTACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAATCGggccagg                                                                                                              <  1:563132/140‑1 (MQ=255)                           cGATACCACCGTTGTATGCTTACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAATCGggccagg                                                                                                              >  2:922659/1‑140 (MQ=255)                             aTACCACCGTTGTATGCCTACGTAATCCGCCCCAAGTACTATGACCAACGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAATCGggccaggcc                                                                                                            <  2:475879/140‑1 (MQ=255)                              tagcaccGTTGTATGCTTCCGTAATCCGCTCCAAATACGATGACCAATGGCGGAAGACATCGCGCTCGCAACCACATCAAGCGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAATCGggccaggcca                                                                                                           <  2:250896/137‑1 (MQ=255)                              tACCACCGTTGTATGCTTACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGACGAAATCAggccaggcca                                                                                                           >  1:250896/1‑140 (MQ=255)                                ccaccGTTGTATGCTTACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCCGTCTGGCGAAGAAATCGGGCCAGGCCAGt                                                                                                         >  1:599957/1‑140 (MQ=255)                                  accGTTGTATGCTTACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTGATATCGGTCTGACGAAGAAATCGGGCCAGGCCAGTGa                                                                                                       >  1:612819/1‑140 (MQ=255)                                  accGTTGTATGCTTACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGa                                                                                                       >  1:575623/1‑140 (MQ=255)                                    cGTTGTATGCTTACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCTCCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATa                                                                                                     >  1:1431654/1‑140 (MQ=255)                                    cGTTGTATGCTTACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAAt                                                                                                                       <  2:1277834/122‑1 (MQ=255)                                    cGTTGTATGCTTACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAAt                                                                                                                       >  1:1277834/1‑122 (MQ=255)                                        gTATGCTTACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAATCGggccaggcca                                                                                                           <  2:207304/130‑1 (MQ=255)                                        gTATGCTTACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAATCGggccaggcca                                                                                                           >  1:207304/1‑130 (MQ=255)                                          aTGCTTACGTAATCCGCACCAAATACGATGATCAATGGCGGAATACATGGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGAt                                                                                               <  2:536057/140‑1 (MQ=255)                                              ttACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGa                                                                                           >  2:157744/1‑140 (MQ=255)                                                aCGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGaaa                                                                                         >  2:1529058/1‑140 (MQ=255)                                                aCGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCAAAGAAATAGGGGCAAGCCAGTAATAAAAGATCTGaaa                                                                                         >  1:415977/1‑140 (MQ=255)                                                   tAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGaaaaaa                                                                                      <  2:599957/140‑1 (MQ=255)                                                     aTCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAAc                                                                                    >  1:1215201/1‑140 (MQ=255)                                                     aTCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAAc                                                                                    >  1:1188972/1‑140 (MQ=255)                                                          ccccAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGt                                                                               >  1:68897/1‑140 (MQ=255)                                                          ccccAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGt                                                                               <  1:1529058/140‑1 (MQ=255)                                                          ccccAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGt                                                                               <  2:263366/140‑1 (MQ=255)                                                          ccccAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGt                                                                               >  1:263366/1‑140 (MQ=255)                                                          ccccAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGt                                                                               >  2:1396981/1‑140 (MQ=255)                                                            ccAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGTAGAAATCGAGCCGGGCAAGTGATAAGAGATGTGAAAAAAACAGCGTaa                                                                             >  2:418709/1‑140 (MQ=255)                                                            ccAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTaa                                                                             >  1:646721/1‑140 (MQ=255)                                                              aaaTACGATGACCAATGGCGGACTACATCGCGCCCTCAATCACGACAACCGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAAc                                                                           <  2:612819/140‑1 (MQ=255)                                                               aaTACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGGTAAGAGATCTGAACAAAACAGCGTAAACa                                                                          >  1:603293/1‑140 (MQ=255)                                                                aTACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAg                                                                         <  2:1215201/140‑1 (MQ=255)                                                                     aTGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGaaaaaa                                                                                      <  1:1396948/122‑1 (MQ=255)                                                                     aTGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGaaaaaa                                                                                      >  2:1396948/1‑122 (MQ=255)                                                                     aTGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTgggg                                                                    <  1:922659/140‑1 (MQ=255)                                                                      tGACCAATTGCGGAATCGATCGCGCCCGCAATCCCCACATACGCACCGGGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGt                                                                   <  2:687096/140‑1 (MQ=255)                                                                       gACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTggg                                                                     <  1:1253276/137‑1 (MQ=255)                                                                       gACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTggg                                                                     >  2:1253276/1‑137 (MQ=255)                                                                       gACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTg                                                                  >  2:173078/1‑140 (MQ=255)                                                                        aCCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTg                                                                  >  2:1235890/1‑139 (MQ=255)                                                                        aCCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTg                                                                  <  1:1235890/139‑1 (MQ=255)                                                                        aCCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGa                                                                 >  2:1242016/1‑140 (MQ=255)                                                                            aTGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGt                                                             >  1:1333532/1‑140 (MQ=255)                                                                                 ggAATACATCGCGCCCCTAACCACGCCAAAGGCCCCGAGTTAACCTAACAGGTTTAATATCGGTCTGGCGAAGAAATCGTGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGtgatga                                                        <  2:756899/140‑1 (MQ=255)                                                                                  gAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTgatgat                                                       >  2:370/1‑140 (MQ=255)                                                                                  gAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTgatgat                                                       >  1:873921/1‑140 (MQ=255)                                                                                      aCATCGCGCCCGCAACCACGACAAAGGCACCGAGATACCCTAAAAGGTTTAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATcgcg                                                   <  1:418709/140‑1 (MQ=255)                                                                                      aCATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATcgcg                                                   <  1:157744/140‑1 (MQ=255)                                                                                       cATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAATAAAACAGAGTAAACAGTGGGGCGAGCGTGATGATCgcgc                                                  >  2:715182/1‑140 (MQ=255)                                                                                       cATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCgcgc                                                  >  2:1486654/1‑140 (MQ=255)                                                                                         tCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAGACAATGTGGTGAGGGTGATGATCGCGCTc                                                >  1:491019/1‑140 (MQ=255)                                                                                          cgcgCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCa                                               >  1:1342717/1‑140 (MQ=255)                                                                                             gcCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCt                                            >  1:280934/1‑140 (MQ=255)                                                                                              cccGCAACCACGACAAATGCACCGAGATAACGTAAAAGGTTTAATATCCGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTg                                           <  2:646721/140‑1 (MQ=255)                                                                                               ccGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGAAAACAGTGGGGGGAGCGTGATGATCGCGCTCACCTgc                                          >  1:436966/1‑140 (MQ=255)                                                                                                          aCAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCa                               <  1:259017/140‑1 (MQ=255)                                                                                                          aCAAACGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCa                               <  1:1466343/140‑1 (MQ=255)                                                                                                            aaaCGCACCGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGc                             <  2:913094/140‑1 (MQ=255)                                                                                                                  aCCGAGATAACCTAAAAGGTTTAATATCGGTATGGCGAACAAATCGGGACAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCa                       <  2:575623/140‑1 (MQ=255)                                                                                                                    cGAGATAACCTAAAAGGTTTAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATc                     >  1:214329/1‑140 (MQ=255)                                                                                                                     gagaTAACCTAAAAGGTTTAATCTCGGTCTGCCGACGAAGTCGGGCCACGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCg                    <  2:491019/140‑1 (MQ=255)                                                                                                                                aaaGGTTTAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCAGCTGCGCTGCCTGCCAGCGAGCGATCGCTTCCGCCAgg         >  1:439582/1‑140 (MQ=255)                                                                                                                                aaaGGTTTAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAgg         >  1:824195/1‑140 (MQ=255)                                                                                                                                aaaGGTTTAATATCGGTCTGGCCAAGAAATCCGGCTAGGCCAGTGATATAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAgg         <  2:436966/140‑1 (MQ=255)                                                                                                                                 aaGGTTTAATATCGGTGGGGCGGAGAAATCGGGTCAGGCGAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAggg        <  2:725392/140‑1 (MQ=255)                                                                                                                                  aGGTTTAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAgg         >  1:846628/1‑138 (MQ=255)                                                                                                                                  aGGTTTAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAgg         <  2:846628/138‑1 (MQ=255)                                                                                                                                   ggTTTAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGgcg      <  1:1486654/140‑1 (MQ=255)                                                                                                                                   ggTTTAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGgcg      <  2:280934/140‑1 (MQ=255)                                                                                                                                    gTTTAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGgcgc     >  1:907814/1‑140 (MQ=255)                                                                                                                                     tttAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGcc    >  2:1088623/1‑140 (MQ=255)                                                                                                                                      ttAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCa   >  1:208519/1‑140 (MQ=255)                                                                                                                                       tAATATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGGGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCat  >  1:361881/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                         GTAAATCATTCGCCTGCGCGGGGTTGCGATACCACCGTTGTATGCTTACGTAATCCGCCCCAAATACGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCAT  >  NC_000913/3331765‑3332038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑