Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,344,165	A→G	A74A (GCA→GCG)	lptB →	lipopolysaccharide transport system ATP binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,344,165	0	A	G	100.0%	239.3 / NA	68	A74A (GCA→GCG)	lptB	lipopolysaccharide transport system ATP binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/33);  total (35/33)

AAATTGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTGCTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACTTGTCTGCTGAACAACGTGAAGACCGCGCGAAC  >  NC_000913/3344032‑3344300                                                                                                                                      |                                                                                                                                        aaaTTGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTGCTGCCTCTGCATGcgcgcgcg                                                                                                                                   <  2:862622/140‑1 (MQ=255)   aTTGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATCACGATATTAGTTTGATGCCTCGGCATGCGcgcgcgcg                                                                                                                                 >  1:454614/1‑140 (MQ=255)    ttGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTGCTGCCTCTGCATgcgcgcgcgcgc                                                                                                                                >  2:341531/1‑140 (MQ=255)     tGTCGGTCTGCTGGGTCCAAACAGTACCGGTAAGACCCCCACTTTCTACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTGCTGCCTCTGCATGCGCGCGCGcgcc                                                                                                                               <  1:564404/140‑1 (MQ=255)     tGTCGGTCTGCTGGGGCCAAACCGTGGCGGTGAGAACCCGACTTTCTATATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTGCTGCCTCTGCATGCGCGCGCGcgcc                                                                                                                               <  2:555206/140‑1 (MQ=255)      gTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTGCTGCCTCTGCATGCGCGCGCGcgccg                                                                                                                              <  1:1076174/140‑1 (MQ=255)      gTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTGCTGCCTCTGCATGCGCGCGCGcgccg                                                                                                                              <  2:1293975/140‑1 (MQ=255)       tCGGTCTGCTGGGGCCAAACGGCGCCGGTCAGACCACTACTTTCTACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTGCTGCCTCTGCATGCGCGCGCGcgccgc                                                                                                                             <  1:421831/140‑1 (MQ=255)               ctgGGGCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGTTGTAGGCATTGTGCCGCGCGATGTGGGCAACATCATTATTGATGATGACGATATCAGTCTGCTGCCTCTGCATGCGCGCGCGCGCCGCGGTATCgg                                                                                                                     <  1:1180949/140‑1 (MQ=255)               ctgGGGCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTGCTGCCTCTGCATGCGCGCGCGCGCCGCGGTATCgg                                                                                                                     >  1:1052765/1‑140 (MQ=255)                   ggCCAAACGGTGCCGGTTAGACTACCACTTTCTACCTGGATGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTGCTGCCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTAt                                                                                                                 <  2:425202/140‑1 (MQ=255)                   ggCCAAACGGTGCCGCTAAGACAACCACTATCTACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTGCTGCCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTAt                                                                                                                 <  1:496226/140‑1 (MQ=255)                       aaaCGGTGCCGGTAAGACCACCACTTTCTACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTGCTGCCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGc                                                                                                             <  1:839083/140‑1 (MQ=255)                              gCCGGTAAGACCACCACTTTCTACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTGCTGCCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGa                                                                                                      <  2:43222/140‑1 (MQ=255)                                cGGTAAGACCACCACTTTCTACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTGCTGCCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAg                                                                                                    <  2:1056093/140‑1 (MQ=255)                                  gTAAGACCACCACTTTCTACATGGTTGTAGACATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTGCTGCCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGcc                                                                                                  >  2:1391472/1‑140 (MQ=255)                                   tAAGACCACCACTTTCTACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTGCTGCCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCt                                                                                                 <  1:1375435/140‑1 (MQ=255)                                                   tACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTGCTGCCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCt                                                                                 >  2:987531/1‑140 (MQ=255)                                                     acaggTTGTAGGCATTGTGCCGGGCCATGAGGGCAACTTCATTATTGATGATGACGATATCAGTCTGCTGCCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCa                                                                               <  1:512743/137‑1 (MQ=255)                                                         gTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTGCTGCCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTTTCTCCGACAGGAAGCCTCCATTGTCCGTCGTCTCAGCGt                                                                           >  2:715969/1‑140 (MQ=255)                                                         gTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTGCTGCCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCa                                                                               <  2:62384/136‑1 (MQ=255)                                                         gTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTGCTGCCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCa                                                                               >  1:62384/1‑136 (MQ=255)                                                             tAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTGCTGCCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTAc                                                                       >  1:1228153/1‑140 (MQ=255)                                                               ggCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTGCTGCCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCa                                                                               >  2:1365148/1‑130 (MQ=255)                                                               ggCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTGCTGCCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCa                                                                               <  1:1365148/130‑1 (MQ=255)                                                                gCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTGCTGCCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGAt                                                                    <  2:1466442/140‑1 (MQ=255)                                                                 cATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTGCTGCCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATa                                                                   <  2:1130899/140‑1 (MQ=255)                                                                  aTTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTGCTGCCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATaa                                                                  >  2:310173/1‑140 (MQ=255)                                                                  aTTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTGCTGCCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATaa                                                                  >  1:315032/1‑140 (MQ=255)                                                                    tgtgCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTGCTGCCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATGTGCCACAGGAAGACTCCATTTTCCGCCGCCTCAGCGTTTACGAtctgt                                                                >  2:512743/1‑136 (MQ=255)                                                                    tgtgCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTGCTGCCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGttt                                                                         >  1:1463357/1‑131 (MQ=255)                                                                    tgtgCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTGCTGCCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGttt                                                                         <  2:1463357/131‑1 (MQ=255)                                                                    tgtgCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTGCTGCCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGAt                                                                    <  1:4894/136‑1 (MQ=255)                                                                    tgtgCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTGCTGCCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGAt                                                                    >  2:4894/1‑136 (MQ=255)                                                                    tgtgCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTGCTGCCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAAcc                                                                >  1:198142/1‑140 (MQ=255)                                                                    tgtgCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTGCTGCCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAAcc                                                                >  1:220214/1‑140 (MQ=255)                                                                    tgtgCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTGCTGCCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAAcc                                                                >  2:1094905/1‑140 (MQ=255)                                                                    tgggCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTGTTGTCTCGGCATGTGCGCGCTCGCCGCGGTATCGGCTATCGGCCACAGGAAGCCTCCAGTTTCGGTCGCCTCAGCGTTTACGATAAcc                                                                >  2:876019/4‑140 (MQ=255)                                                                     gtgCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTGCTGCCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCt                                                               <  1:1391472/140‑1 (MQ=255)                                                                                   gggCAACATCATTATTGTTGATGACGATATCCGTCTGCTCCCTCTGCACGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGc                                                 <  2:454614/140‑1 (MQ=255)                                                                                          atcatTATTGATGATGACGATATCAGTCTGCTGCCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCg                                          >  1:907275/1‑140 (MQ=255)                                                                                          atcatTATTGATGATGACGATATCAGTCTGCTGCCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTGCCGTCGCCTCAGAGTTGACGATAACGTGATAGCGGTACTGCAAATTCg                                          >  2:431118/1‑140 (MQ=255)                                                                                              ttattGATGATGACGATATCAGTCTGCTGCCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTgac                                      >  2:1002214/1‑140 (MQ=255)                                                                                                 ttGATGATGACGATATCAGTCTGCTGCCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTgacgac                                   <  1:1372505/140‑1 (MQ=255)                                                                                                   gatgatgaCGATATCAGTCTGCTGCCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACtt                                 <  1:341531/140‑1 (MQ=255)                                                                                                        tgaCGATATCAGTCTGCTGCCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACTTGTctg                            >  2:184519/1‑140 (MQ=255)                                                                                                        tgaCGATATCAGTCTGCTGCCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACTTGTctg                            >  2:29029/1‑140 (MQ=255)                                                                                                         gaCGATATCAGTCTGCTGCCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACTTGTctgc                           >  2:801898/1‑140 (MQ=255)                                                                                                              tatCAGTCTGCTGCCTCTGCATGCGGGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGGCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACTTGTCTTCTGaac                      <  2:884458/140‑1 (MQ=255)                                                                                                              tatCAGTCTGCTGCCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGGTGGCGATGGTGCAAATGCGTGACGACTTGTGTGCTGaac                      >  2:744212/1‑140 (MQ=255)                                                                                                              tatCAGTCTGCTGCCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACTTGTCTGCTGaac                      <  1:1094905/140‑1 (MQ=255)                                                                                                              tatCAGTCTGCTGCCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACTTGTCTGCTGaac                      <  1:876019/140‑1 (MQ=255)                                                                                                              tatCAGTCTGCTGCCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACTTGTCTGCTGaac                      <  2:315032/140‑1 (MQ=255)                                                                                                              tatCAGTCTGCTGCCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACTTGTCTGCTGaac                      <  2:220214/140‑1 (MQ=255)                                                                                                              tatCAGTCTGCTGCCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACTTGTCTGCTGaac                      <  2:198142/140‑1 (MQ=255)                                                                                                              tatCAGTCTGCTGCCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACTTGTCTGCTGaac                      <  1:987531/140‑1 (MQ=255)                                                                                                              tatCAGTCTGCTGCCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACTTGTCTGCTGaac                      <  2:1349844/140‑1 (MQ=255)                                                                                                              tatCAGTCTGCTGCCTCTGCATGCGCGCGCGCGCCCCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACTTGTCTGCTGaac                      <  2:491576/140‑1 (MQ=255)                                                                                                              tatCAGTCTGCTGCCACTGCATCCGCGCGCGCGCCGCCGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACTTGTCTGCTGaac                      <  1:451031/140‑1 (MQ=255)                                                                                                                   gTCTGTTGCCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACTTGTCTGCTGAACAACGt                 >  1:148608/1‑140 (MQ=255)                                                                                                                      tgctgcCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACTTGTCTGCTGAACAACGTGaa              >  1:1116951/1‑140 (MQ=255)                                                                                                                        ctgcCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACTTGTCTGCTGaac                      <  2:934101/130‑1 (MQ=255)                                                                                                                        ctgcCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACTTGTCTGCTGaac                      >  1:934101/1‑130 (MQ=255)                                                                                                                        ctgcCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACTTGTCTGCTGAACAACGTGAAGa            >  2:995138/1‑140 (MQ=255)                                                                                                                        ctgcCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACTTGTCTGCTGAACAACGTGAAGa            >  1:266820/1‑140 (MQ=255)                                                                                                                        ctgcCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGAGGGCGGTACCGCATAATCATGACGACTTGTCTACTGAACAACGTGAAGa            >  2:757186/1‑140 (MQ=255)                                                                                                                        ctgcCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGAGGGCGGTACAGCAGATTAGTGACGACGTGTCGGCTGAACAACGTGAAGa            >  2:451031/1‑140 (MQ=255)                                                                                                                         tgcCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACTTGTCTGCTGAACAACGTGAAGAc           >  2:827049/1‑140 (MQ=255)                                                                                                                         tgcCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACTTGTCTGCTGAACAACGTGAAGAc           >  1:919763/1‑140 (MQ=255)                                                                                                                         tgcCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACTTGTCTGCTGAACAACATGAAGAc           >  2:344380/1‑140 (MQ=255)                                                                                                                          gcCTCTGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACTTGTCTGCTGAACAACGTGAAGAcc          >  1:356690/1‑140 (MQ=255)                                                                                                                              ctGCATGCGCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACTTGTCTGCTGAACAACGTGAAGACcgcgc      >  2:280110/1‑140 (MQ=255)                                                                                                                                  atgcgCGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACTTGTCTGCTGAACAACATGAAGACCGCGCGAAc  <  1:344380/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                        AAATTGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTGCTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACTTGTCTGCTGAACAACGTGAAGACCGCGCGAAC  >  NC_000913/3344032‑3344300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑