Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,375,656	A→G	H263R (CAC→CGC)	dcuD →	putative transporter DcuD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,375,656	0	A	G	100.0%	269.2 / NA	78	H263R (CAC→CGC)	dcuD	putative transporter DcuD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/39);  total (39/39)

TTTTGACAAAAAAGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGC‑TCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCACGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCGCAAGCATAACTTGCGCGAAACAATGGACGATGTGCAGGCGTTTTTTGACG  >  NC_000913/3375522‑3375790                                                                                                                                        |                                                                                                                                       ttttGAAAAAAAAGAAATAAATTCCAATCACTAACCGGACGA‑CAAAAATCTTCTCGATAATGCCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCg                                                                                                                                   <  2:395166/140‑1 (MQ=255) ttttAAAAAAAAAGATAAAAATTTCAATCACTAACTGGCCGAGCAAAAAGC‑CCTCAATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCg                                                                                                                                   <  2:439714/140‑1 (MQ=255)  tttGACAAAAAAGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGC‑TCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCgg                                                                                                                                  >  1:970284/1‑140 (MQ=255)        aaaaaaaaaaaaaaTATAAATCAGGACAAGGAAAAGCAAAAAGC‑TCTCAATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGAt                                                                                                                            <  2:181037/140‑1 (MQ=255)        aaaaaaaaaaaaATTATCAATCACGACCGGGCAGAACAAAAACC‑GCTCGATAAGGTCCCTCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGAt                                                                                                                            <  2:366959/140‑1 (MQ=255)        aaaaaaGATAAAAATATGAATCGCGAACAGGCAGAGAAAAAAGC‑TCCGCATAATGCCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGAt                                                                                                                            <  2:752633/140‑1 (MQ=255)         aaaaaGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGC‑TCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGAAGCTCGGCTCGCAGTGCCTCGCCCGCGTCGGGCTGATg                                                                                                                           >  1:497324/1‑140 (MQ=255)          aaaaGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGC‑TCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGc                                                                                                                          >  2:1474418/1‑140 (MQ=255)             aGATAAAAAAATCAATCACGAACAGGAAGAGCAAAAGGC‑TCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGt                                                                                                                       <  2:219194/140‑1 (MQ=255)              aaaaaaaaTATAAACCACAAACGGAAAGAAAAAAAGGC‑CCTCGATAATCTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTc                                                                                                                      <  2:324923/137‑1 (MQ=255)                 aaaaaTATAAACCACATACAGATAGAGAAACGAGC‑GCTCAATAATGCCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGa                                                                                                                   <  2:728016/140‑1 (MQ=255)                  aaaaTACCAATCCCGAAGAGGAAGAGAAAAATGC‑TTTTGTTAATGTCCGGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGaa                                                                                                                  <  2:659565/140‑1 (MQ=255)                         caatcaCGAACCGGCAGAGCAAAAAGG‑TCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACGTGTGATGCCGTGAATCCTGATGCTCAGCCCGTAGATCCGCGCACGCAGCGGACTGAAACAATCAAAACTGCATc                                                                                                           >  2:396048/1‑140 (MQ=255)                            tcaCGAACAGGCAGAGCAAAAAGC‑TCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCtgg                                                                                                        <  1:762408/140‑1 (MQ=255)                            tcaCGAACAGGCAGAGAAAAAAGC‑TCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCtgg                                                                                                        <  2:1323954/140‑1 (MQ=255)                            ccaCGAACGGGCAGAAACAAAATT‑TCTCGATGAGTTCCCTCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCtgg                                                                                                        <  2:703510/139‑1 (MQ=255)                                gAACAGGCAGAGCAAAAAGC‑TCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCtggtggt                                                                                                    >  1:121271/1‑140 (MQ=255)                                  aCAGGCAGAGCAAAACGC‑TCTCCATTATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCtggtggtgg                                                                                                  <  1:482564/140‑1 (MQ=255)                                  aCAGGCAGAGAAAAAAGC‑TCTCAATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCtggtggtgg                                                                                                  <  1:1126818/140‑1 (MQ=255)                                    agaatgagaAAAAAGC‑TCTCAATAACTTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCTGgtggtggtg                                                                                                <  2:1548364/135‑1 (MQ=255)                                          agCAAAAAGC‑TCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCAt                                                                                                            <  1:269852/122‑1 (MQ=255)                                          agCAAAAAGC‑TCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCAt                                                                                                            >  2:269852/1‑122 (MQ=255)                                          agCAAAAAGC‑TCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTa                                                                                          >  1:1165265/1‑140 (MQ=255)                                          agCAAAAAGC‑TCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTa                                                                                          >  2:16902/1‑140 (MQ=255)                                          agCAAAAAGC‑TCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTa                                                                                          >  2:857077/1‑140 (MQ=255)                                          agCAAAAAGC‑TCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTa                                                                                          >  2:936042/1‑140 (MQ=255)                                          agCAAAAAGC‑TCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCGGATGCAGACAGAACGGCATCTCGTGGTGGTGATGTTa                                                                                          >  1:697190/1‑140 (MQ=255)                                          agCAAAAAGC‑TCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGACTGATGCAGTCAGAACGGCATCTGGTGGTGGTGATGTTa                                                                                          >  2:575226/1‑140 (MQ=255)                                            aaaaaaGC‑TCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACt                                                                                        <  1:16902/139‑1 (MQ=255)                                            aaaaaaGC‑TCTCAATAATCCCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACt                                                                                        <  2:88570/139‑1 (MQ=255)                                               aaaGC‑TCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAg                                                                                     >  2:1460500/1‑140 (MQ=255)                                                aaGC‑TCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTGGGGCTGATACAGGCAGGACTGCATCGGGTGGTGGTGATGTTACTGAGt                                                                                    >  2:423544/1‑140 (MQ=255)                                                aaGC‑TCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAATTGCGTCAGGTGGCAGTGATGTTACTGAGt                                                                                    >  1:644307/1‑140 (MQ=255)                                                 aGC‑TCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGtt                                                                                   <  2:1409546/140‑1 (MQ=255)                                                  gC‑TCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGttt                                                                                  <  1:1474418/140‑1 (MQ=255)                                                       tcGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGGACTGCATTTGGGGGGGGTGATATTACTGAGTTTGACt                                                                              >  1:686276/1‑140 (MQ=255)                                                       tcGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACt                                                                              <  2:160693/140‑1 (MQ=255)                                                       tcGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACt                                                                              >  1:160693/1‑140 (MQ=255)                                                       tcGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACt                                                                              <  2:121271/140‑1 (MQ=255)                                                       ggcaTAATGTCCCGCCGCTCTATTACGCCATTTTCCCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACt                                                                              <  2:970284/137‑1 (MQ=255)                                                          cagaTGTCCCGCCGCTCTATTAAGCCATTTTCCCTCTGAAGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACtgtg                                                                           <  2:492053/137‑1 (MQ=255)                                                            aaTGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGAc                                                                         <  1:934333/140‑1 (MQ=255)                                                              tGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGa                                                                       >  2:1523490/1‑140 (MQ=255)                                                                 cccGCCGCTCTATTACATCATTTTACCTGTGCTGCGGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGAtgt                                                                    <  2:697190/140‑1 (MQ=255)                                                                   cgccgcTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCTGGAGGTGGTGATGTTACCGAGTTTGACTGTGACGAtgttt                                                                  >  1:577048/1‑140 (MQ=255)                                                                     ccgcTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGGTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACAGCATCTGGGGGGGGAGGTGTTACTGAATTTGTTTGTGACTAtgtttgt                                                                >  2:755057/1‑140 (MQ=255)                                                                        ctctATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGATGATGTTTCTGAGTGTGACTGTGACGAtgtttgtctg                                                             >  1:492053/1‑137 (MQ=255)                                                                         tctATTACGCCATTTTACCTGTCATGCCGTTAATCCTGATGCTCGGCTTGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAg                                                            <  2:627624/140‑1 (MQ=255)                                                                                 gCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCg                                                    <  2:1489127/140‑1 (MQ=255)                                                                                 gCCATTTTACCTGTGATGCCGTCAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCg                                                    <  1:1212004/140‑1 (MQ=255)                                                                                  ccATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTTGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCGc                                                   >  2:264027/1‑140 (MQ=255)                                                                                  ccATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCGc                                                   >  1:1327654/1‑140 (MQ=255)                                                                                   cATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCGCa                                                  >  2:345827/1‑140 (MQ=255)                                                                                   cATTTTACCTGTGATCCCGTTACTCCTGCTGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCGCa                                                  <  1:755057/140‑1 (MQ=255)                                                                                     ttttACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGGATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCGCa                                                  >  2:1223084/1‑138 (MQ=255)                                                                                     ttttACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGGATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCGCa                                                  <  1:1223084/138‑1 (MQ=255)                                                                                     ttttACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCGCAAg                                                >  2:1010610/1‑140 (MQ=255)                                                                                     ttttACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCGCAAg                                                >  2:216699/1‑140 (MQ=255)                                                                                             gtgATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCGCAAGCATAACtt                                        >  2:182791/1‑140 (MQ=255)                                                                                             gtgATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCGCAAGCATAACtt                                        >  1:192585/1‑140 (MQ=255)                                                                                                aTGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCGCAAGCATAACTTgcg                                     <  2:1079239/140‑1 (MQ=255)                                                                                                aTGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCGCAAGCATAACTTgcg                                     <  1:182791/140‑1 (MQ=255)                                                                                                aTGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGGTCTTCCCCAAGCATAACTTgcg                                     >  1:595041/1‑140 (MQ=255)                                                                                                 tGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCGCAAGCATAACTTgcgc                                    >  1:937600/1‑140 (MQ=255)                                                                                                  gCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCGCAAGCATAACTTGcgcg                                   >  2:104823/1‑140 (MQ=255)                                                                                                      ttAATCCTGATGCTCGGGTCGCAGTTCCTCGTCCGGGTCTGGCTGATGTAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCGCAAGCATAACTTGCGCGAAAc                               <  2:497324/140‑1 (MQ=255)                                                                                                      ttAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCGCAAGCATAACTTgcgct                                   >  1:55912/1‑135 (MQ=255)                                                                                                      ttAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCGCAAGCATAACTTgcgct                                   <  2:55912/136‑2 (MQ=255)                                                                                                        aaTCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCGCAAGCATAACTTGCGCGAAAc                               <  2:29017/138‑1 (MQ=255)                                                                                                        aaTCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCGCAAGCATAACTTGCGCGAAAc                               >  1:180539/1‑138 (MQ=255)                                                                                                        aaTCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCGCAAGCATAACTTGCGCGAAAc                               <  2:180539/138‑1 (MQ=255)                                                                                                        aaTCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCGCAAGCATAACTTGCGCGAAAc                               >  1:29017/1‑138 (MQ=255)                                                                                                         aTCCTGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCGCAAGCATAACTTGCGCGAAACAAt                            <  1:1460500/140‑1 (MQ=255)                                                                                                             tGATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGGGTTCTTCGGCAGGCATAACTTACGCGAAACAATGGAc                        >  1:457494/1‑140 (MQ=255)                                                                                                              gATGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCGCAAGCATAACTTGCGCGAAACAATGGACg                       <  2:1327654/140‑1 (MQ=255)                                                                                                              gATGCTCGCCTCGCTGGTCCTCCCCCGCGTCTGACCGATCCAGTCAGACCTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCGCAAGCATAACTTGCGCGAAACAATGGACg                       <  2:716659/140‑1 (MQ=255)                                                                                                               aTGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCGCAAGCATAACTTGCGCGAAACAATGGACGa                      >  2:312059/1‑140 (MQ=255)                                                                                                               aTGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCGCAAGCATAACTTGCGCGAAACAATGGACGa                      >  1:1347719/1‑140 (MQ=255)                                                                                                               aTGCTCGGCTCGCTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGATCTTCCGAAAGCATGACTTGCACGAAACAATGGACGa                      >  1:716659/1‑140 (MQ=255)                                                                                                                           cTGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCGCAAGCATAACTTGCGCGAAACAATGGACGATGTGCAGGCGtt          >  1:999101/1‑140 (MQ=255)                                                                                                                            tGTTCCTCGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCGCAAGCATAACTTGCGCGAAACAATGGACGATGTGCAGGCGttt         >  1:931668/1‑140 (MQ=255)                                                                                                                                   cGCCCGCGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCGCAAGCATAACTTGCGCGAAACAATGGACGATGTGCAGGCGTTTTTTGACg  >  1:1494981/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                       TTTTGACAAAAAAGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGC‑TCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCACGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCGCAAGCATAACTTGCGCGAAACAATGGACGATGTGCAGGCGTTTTTTGACG  >  NC_000913/3375522‑3375790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑