Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,465,960 T→C C140R (TGT→CGT)  gspO → Type II secretion system prepilin peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,465,9600TC98.7% 253.0 / NA 74C140R (TGT→CGT) gspOType II secretion system prepilin peptidase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (36/37);  total (36/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACCGCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTG  >  NC_000913/3465823‑3466095
                                                                                                                                         |                                                                                                                                       
aTTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACCGCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGgcgt                                                                                                                                       >  1:778274/1‑140 (MQ=255)
  tttCGCACTCAGCTCCTGCCCGACAAACTCACCTTACCGCTGCTCTGGCTTGGCTTGGTATTTAATTCGCAGTATGGATTAATTGATTTACATGATGCGGTTCACGGCGCGGTAACGGGCTATGGGGTGCTGTGgcgtgt                                                                                                                                     >  1:547456/1‑140 (MQ=255)
  tttCGCACTCAGCTCCTGCCCGACAAACTCACCTTACCGCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTATTTGATTTACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGgcgtgt                                                                                                                                     >  1:938578/1‑140 (MQ=255)
  tttCGCACTCAGCTCCTGCCCGACAAACTCACCTTACCGCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGgcgtgt                                                                                                                                     >  2:1115435/1‑140 (MQ=255)
    tCGCACTCAGCTCCTGCCCGACAAACTCACCTTACCGCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGttt                                                                                                                                   >  1:1434660/1‑140 (MQ=255)
         cTCAGCTCCTGCCCGACAAACTCACCTTACCGCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTgg                                                                                                                              <  2:1230268/140‑1 (MQ=255)
          tCAGCTCCTGCCCGACAAACTCACCTTACCGCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTggg                                                                                                                             >  2:955072/1‑140 (MQ=255)
            aGCTCCTGCCCGACAAACTCACCTTACCGCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGc                                                                                                                           <  2:1429563/140‑1 (MQ=255)
              cTCCTGCCCGACAAAGTCACCTTACCGCTACTCTGGCGTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGt                                                                                                                         <  1:544598/140‑1 (MQ=255)
                   gCCCGACAAACTCACCTTACCGCTGATCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGt                                                                                                                    <  1:647461/140‑1 (MQ=255)
                      cGACAAACTCACCTTACCGCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAg                                                                                                                 >  2:325228/1‑140 (MQ=255)
                      cGACAAACTCACCTTACCGCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACCGGGGCGTCTGGGTAg                                                                                                                 >  1:607681/1‑140 (MQ=255)
                        aCAAACTCACCTTACCGCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGtt                                                                                                               >  2:1088591/1‑140 (MQ=255)
                        aCAAACTCACCTTACCGCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGGTAGtt                                                                                                               >  1:1464177/1‑140 (MQ=255)
                           aaCTCACCTTACCGCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGt                                                                                                            <  2:1434660/140‑1 (MQ=255)
                           aaCTCACCTTACCGCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGGGCTGTGGCGTGATTCCTGGGGCGTCTGGTTAGTTTGt                                                                                                            >  1:568040/1‑140 (MQ=255)
                             cTCACCTTACCGCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTca                                                                                                          <  1:325228/140‑1 (MQ=255)
                                  cTTACCGCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAag                                                                                                     <  2:72030/140‑1 (MQ=255)
                                       cGCTGCTCTGGCTTGGCTTGGTTTTTAATTCTCAGTATGGATTAATTGTTTTACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGa                                                                                                <  2:547456/140‑1 (MQ=255)
                                          tgctCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTg                                                                                             >  1:65134/1‑140 (MQ=255)
                                            ctctGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAag                                                                                                     <  2:167932/130‑1 (MQ=255)
                                            ctctGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAag                                                                                                     >  1:167932/1‑130 (MQ=255)
                                              ctGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCt                                                                                         <  1:140921/140‑1 (MQ=255)
                                                 gcttggcttgGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACg                                                                                      <  1:955072/140‑1 (MQ=255)
                                                      gcttggTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTGTGGTTGGGTTGTCACACAGAGGGATTGGGCAACGGTGAt                                                                                 >  2:687163/1‑140 (MQ=255)
                                                          ggTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCa                                                                             <  2:962804/140‑1 (MQ=255)
                                                           gTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACgg                                                                                     >  1:48403/1‑131 (MQ=255)
                                                           gTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACgg                                                                                     <  2:48403/131‑1 (MQ=255)
                                                                tAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCGGGTTAGTTTGTCACAAGGAGGGATTGGGCTAAGGTGATTTCAAGCTAc                                                                       >  2:670479/1‑140 (MQ=255)
                                                                     cgcAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGcc                                                                  >  2:1327661/1‑140 (MQ=255)
                                                                      gcAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCg                                                                 >  1:764412/1‑140 (MQ=255)
                                                                        aGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCt                                                               >  1:1404749/1‑140 (MQ=255)
                                                                          tATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGc                                                             <  1:1421111/140‑1 (MQ=255)
                                                                           aTGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCa                                                            <  1:329962/140‑1 (MQ=255)
                                                                           aTGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCa                                                            <  1:1088591/140‑1 (MQ=255)
                                                                               aTTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGgcg                                                        <  2:51393/140‑1 (MQ=255)
                                                                                ttAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGgcgc                                                       <  2:346710/140‑1 (MQ=255)
                                                                                    ttGATTTACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGATGGATTGGGGTACGGTGATTTCAAGCAACTGGCCGCTGCAGGCGCATgg                                                   >  2:513884/1‑140 (MQ=255)
                                                                                    ttGATTTACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTCCGGTGATTTCAAGCTACTGGCCGCTGCAGGCGCATgg                                                   >  1:577254/1‑140 (MQ=255)
                                                                                    ttGATTTACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGCGCATgg                                                   >  2:1510726/1‑140 (MQ=255)
                                                                                    ttGATTTACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGCGCATgg                                                   >  2:157547/1‑140 (MQ=255)
                                                                                    ttGATTTACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGCGCATgg                                                   >  2:1086486/1‑140 (MQ=255)
                                                                                    ttGATTTACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGCGCATgg                                                   >  2:108355/1‑140 (MQ=255)
                                                                                    ttGATTTACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCAACTGGCCGCTGCAGGCGCATgg                                                   >  2:672955/1‑140 (MQ=255)
                                                                                    ttGATTTACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGGCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTGCGGGGATGTCATGCGACTGGCTGCTGCAGGCGCATgg                                                   >  1:501313/1‑140 (MQ=255)
                                                                                    ttGATTTACATGATGCGGTTTACGGCGCGGTAGCCGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGgcgc                                                       >  2:265717/1‑136 (MQ=255)
                                                                                    ttGATTTACATGATGCGGTTTACGGCGCGGTAGCCGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGgcgc                                                       <  1:265717/136‑1 (MQ=255)
                                                                                      gATTTACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGCGCATGgtg                                                 >  1:1330409/1‑140 (MQ=255)
                                                                                        tttACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGCGCATGGtgtg                                               >  1:96171/1‑140 (MQ=255)
                                                                                        tttACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGCGCATGGtgtg                                               >  2:866185/1‑140 (MQ=255)
                                                                                        tttACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGCGCATGGtgtg                                               >  2:1156195/1‑140 (MQ=255)
                                                                                        tttACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGCGATTTCAGGCTACTGGCCGCTGCAGGCGCATGGtgtg                                               >  1:585790/1‑140 (MQ=255)
                                                                                         ttACATGATGCGGTTTAGGGCAAGGTAGCGGTCTATGGGTTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGCGCATGGTGtgg                                              <  2:536404/140‑1 (MQ=255)
                                                                                         ttACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGCGCATGGTGtgg                                              >  1:1218192/1‑140 (MQ=255)
                                                                                               gatgCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCa                                        >  1:155281/1‑140 (MQ=255)
                                                                                                 tgCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGgcg                                                        >  2:1462344/1‑122 (MQ=255)
                                                                                                 tgCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGgcg                                                        <  1:1462344/122‑1 (MQ=255)
                                                                                                 tgCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCaaa                                      >  2:1474058/1‑140 (MQ=255)
                                                                                                 tgCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCaaa                                      >  1:1449517/1‑140 (MQ=255)
                                                                                                 tgCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCaaa                                      >  1:785824/1‑140 (MQ=255)
                                                                                                   cGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGAGGCGTGTTTACTAGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACg                                    <  2:620008/140‑1 (MQ=255)
                                                                                                     gTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGtt                                  <  1:1086486/140‑1 (MQ=255)
                                                                                                     gTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGtt                                  <  1:866185/140‑1 (MQ=255)
                                                                                                        tACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGcc                               <  1:670479/140‑1 (MQ=255)
                                                                                                        tACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGcc                               <  1:672955/140‑1 (MQ=255)
                                                                                                        tACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGcc                               <  1:1115435/140‑1 (MQ=255)
                                                                                                          cGGCGCGGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCaa                             <  1:1510726/140‑1 (MQ=255)
                                                                                                             cgcgGTAGCGGGCTATGGGGTGCTGTGGCGTGTTTGCTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGa                          <  2:577254/140‑1 (MQ=255)
                                                                                                                 gTAGCGGGCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATAct                      <  2:764412/140‑1 (MQ=255)
                                                                                                                    gCGGGCTATGGGGTGCTCTGGCCTGTTTACTTGGGCGTCTGGTTAGTTGGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATActgct                   <  2:585790/140‑1 (MQ=255)
                                                                                                                      gggCTATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGa                 <  2:1404749/140‑1 (MQ=255)
                                                                                                                          tATGGGGTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGc             <  2:938578/140‑1 (MQ=255)
                                                                                                                              gggTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCg         <  2:155281/140‑1 (MQ=255)
                                                                                                                                gTGCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCt       <  1:1420679/140‑1 (MQ=255)
                                                                                                                                  gCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTgg     >  2:1107571/1‑140 (MQ=255)
                                                                                                                                  gCTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTgg     <  1:1107571/140‑1 (MQ=255)
                                                                                                                                   cTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTggg    <  1:1474058/140‑1 (MQ=255)
                                                                                                                                   cTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTggg    <  1:108355/140‑1 (MQ=255)
                                                                                                                                   cTGTGGCGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGCGCATAGGGTGGCTAGCAAACGCTGCCAATGCTACTGCTGATTGCCTCGCTggg    >  2:534297/1‑140 (MQ=255)
                                                                                                                                     gtggcgTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTg  <  1:1327661/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                       
ATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACCGCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGGGCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAGCTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTG  >  NC_000913/3465823‑3466095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑