Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,998,655	A→G	T225A (ACC→GCC)	uvrD →	ssDNA translocase and dsDNA helicase ‑ DNA helicase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,998,655	0	A	G	100.0%	312.8 / NA	88	T225A (ACC→GCC)	uvrD	ssDNA translocase and dsDNA helicase ‑ DNA helicase II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (40/48);  total (40/48)

AGCGTGTGACCGCGCGGGCCTGGTGGACTTCGCCGAGCTGCTGCTGCGCGCTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTCAGCGTTTCCTTAATG  >  NC_000913/3998519‑3998790                                                                                                                                         |                                                                                                                                        aGCGTGTGACCGCGCGGGCCTGGTGGACTTCGCCGAGCTGCTGCTGCGCGCTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCca                                                                                                                                      <  1:1492094/140‑1 (MQ=255)   cGTGTGACCGCGCGGGCCTGGTGGACTTCGCCGAGCTGCTGCTGCGCGCTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCcaac                                                                                                                                    <  2:1065663/140‑1 (MQ=255)   cGTGTGACCGCGCGGGCCTGGTGGACTTCGCCGAGCTGCTGCTGCGCGCTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCcaac                                                                                                                                    <  2:363308/140‑1 (MQ=255)   cGTGTGACCGCGCGGGCCTGGTGGACTTCGCCGAGCTGCTGCTGCGCGCTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCcaac                                                                                                                                    <  1:1404280/140‑1 (MQ=255)    gtgtGACCGCGCGGGCCTGGTGGACTTCGCCGAGCTGCTGCTGCGCGCTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCcaaca                                                                                                                                   <  2:1233037/140‑1 (MQ=255)    gtgtGACCGCGCGGGCCTGGAGGACTTCGCCGAGCTGCTGCTGCGCGCTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTAGAGGACGAATTCCAGGATGCcaaca                                                                                                                                   >  1:523140/1‑140 (MQ=255)     tgtgACCGCGCGGGCCTGGTGGACTTCGCCGAGCTGCTGCTGCGCGCTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCcaac                                                                                                                                    <  1:173381/138‑1 (MQ=255)     tgtgACCGCGCGGGCCTGGTGGACTTCGCCGAGCTGCTGCTGCGCGCTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCcaac                                                                                                                                    >  2:173381/1‑138 (MQ=255)         aCCGCGCGGGCCTGGTGGACTTCGCCGAGCTGCTGCTGCGCGCTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACTAATTTCCGGGTGGACGTATTCAAGGATGCCAACAACAtt                                                                                                                              >  2:402406/1‑140 (MQ=255)         aCCGCGCGGGCCTGGTGGACTTCGCCGAGCTGCTGCTGCGCGCTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACAtt                                                                                                                              <  1:1336173/140‑1 (MQ=255)         aCCGCGCGGGCCTGGTGGACTTCGCCGAGCTGCTGCTGCGCGCTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACAtt                                                                                                                              <  2:1335191/140‑1 (MQ=255)             cgcgGGCCTGGTGGACTTCGCCGAGCTGCTGCTGCGCGCTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGt                                                                                                                          <  2:943479/140‑1 (MQ=255)             cgcgGGCCTGGTGGACTTCGCCGAGCTGCTGCTGCGCGCTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGAAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAg                                                                                                                           >  1:846601/1‑139 (MQ=255)             cgcgGGCCTGGTGGACTTCGCCGAGCTGCTGCTGCGCGCTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGAAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAg                                                                                                                           <  2:846601/139‑1 (MQ=255)              gcgGGCCTGGTGGACTCCGCCGTGCTGCTGTTGCGAGCTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTa                                                                                                                         <  2:712929/140‑1 (MQ=255)                gggCCTGGTGGACTTCGCCGAGCTGCTGCTGCGCGCTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTAcg                                                                                                                       <  2:128369/140‑1 (MQ=255)                gggCCTGGTGGACTTCGCCGAGCTGCTGCTGCGCGCTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTAcg                                                                                                                       <  2:650598/140‑1 (MQ=255)                 ggCCTGGTGGACTTCGCGGAGCTGCTGCTGCCCGCCCACTAGTTGTGGTTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTAcgc                                                                                                                      <  2:410719/140‑1 (MQ=255)                    cTGGTGGACTTCGCCGAGCTGCTGCTGCGCGCTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTACGCGTg                                                                                                                   <  1:1165653/140‑1 (MQ=255)                            cTTCGCCGAGCTGCTGCTGCGCGCTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGTTGGACGAATTCCAGGACGCCAACAAGATTCTGTACGCGTGGATCCGcc                                                                                                           >  2:656489/1‑140 (MQ=255)                            cTTCGCCGAGCTGCTGCTGCGCGCTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGcc                                                                                                           >  2:1044021/1‑140 (MQ=255)                            cTTCGCCGAGCTGCTGCTGCGCGCTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGcc                                                                                                           >  1:1164820/1‑140 (MQ=255)                            cTTCGCCGAGCTGCTGCTGCGCGCTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGcc                                                                                                           >  1:1179551/1‑140 (MQ=255)                            cTTCGCCGAGCTGCTGCTGCGCGCTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGAAGCCAACAACATTCCCTACGCGTGGATCCGcc                                                                                                           >  2:666796/1‑140 (MQ=255)                            cTTCGCCGAGCTGCTGCTGCGCGCTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTACAGGATGACAAGAAAATTCAGTACGCGTGGATCCGct                                                                                                           >  1:500013/1‑139 (MQ=255)                              tCGCCGAGCTGCTGCTGCGCGCTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCctg                                                                                                         >  2:1528127/1‑140 (MQ=255)                               cGCCGAGCTGCTGCTGCGCGCTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCctgc                                                                                                        <  1:86703/140‑1 (MQ=255)                                gCCGAGCTGCTGCTGCGCGCTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCctgct                                                                                                       >  1:350561/1‑140 (MQ=255)                                gCCGAGCTGCTGCTGCGCGCTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCctgct                                                                                                       >  1:1440419/1‑140 (MQ=255)                                 ccGAGCTGCTGCTGCGCGCTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCctgctg                                                                                                      <  2:1164820/140‑1 (MQ=255)                                       tgctgctgcGCGCTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCCTGCTggcgggc                                                                                                <  1:1528127/140‑1 (MQ=255)                                         ctgctgcGCGCTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCCAGCAAAAATGAGTACGCGGGGATCCGCCTGCTGGCGGGCGa                                                                                              >  2:681623/1‑140 (MQ=255)                                            ctgcGCGCTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGacac                                                                                           <  1:29884/140‑1 (MQ=255)                                               cgcgcTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCgg                                                                                        >  1:176322/1‑140 (MQ=255)                                                 cgcTCACGAGTTGTGTCTTAACTAGCCGCATATCCTGTAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCa                                                                                      <  1:681623/140‑1 (MQ=255)                                                         aGTTGTGGCTTAACACGCCGCACATTCTGTAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGtgatg                                                                              <  1:486862/140‑1 (MQ=255)                                                         aGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGtgatg                                                                              <  2:815998/140‑1 (MQ=255)                                                                gCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGAtcgtcg                                                                       <  2:235475/140‑1 (MQ=255)                                                                   tAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGtg                                                                    >  1:1399061/1‑140 (MQ=255)                                                                     aCAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGCCGACACCGCCAAAGTGATGATCGTCGGtgat                                                                  >  2:669529/1‑140 (MQ=255)                                                                     aCAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGCATCCGCCTTCTGGCAGAAGACACCGCCAAAGGGATGATCGTCGGtgat                                                                  >  2:736194/1‑140 (MQ=255)                                                                         gCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATgacg                                                              <  2:1484512/140‑1 (MQ=255)                                                                           cGCATATCCTGCAACCCTACCGGGAACGTGTTACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATgacgac                                                            <  1:666796/140‑1 (MQ=255)                                                                           cGCATATCCTGCAACACTACCGCGAGCGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATgacgac                                                            <  1:736194/140‑1 (MQ=255)                                                                           cGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATgacgac                                                            <  2:1515615/140‑1 (MQ=255)                                                                            gCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTAGGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGAcc                                                           <  2:1327917/140‑1 (MQ=255)                                                                                    tGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATc                                                   >  2:240261/1‑140 (MQ=255)                                                                                    tGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATc                                                   >  2:357116/1‑140 (MQ=255)                                                                                     gCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCt                                                  >  1:81159/1‑140 (MQ=255)                                                                                     gCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCt                                                  >  2:1336772/1‑140 (MQ=255)                                                                                      cAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTa                                                 >  1:1485360/1‑140 (MQ=255)                                                                                       aaCACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTAc                                                >  1:4835/1‑140 (MQ=255)                                                                                            tACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTg                                           >  2:848439/1‑140 (MQ=255)                                                                                              ccGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGgc                                         >  2:249276/1‑140 (MQ=255)                                                                                                gcgAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGgcgc                                       >  1:205740/1‑140 (MQ=255)                                                                                                 cgAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGcgcg                                      >  1:926423/1‑140 (MQ=255)                                                                                                 cgAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGcgcg                                      <  2:4835/140‑1 (MQ=255)                                                                                                  gAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCgg                                     <  2:54209/140‑1 (MQ=255)                                                                                                   aaCGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCggg                                    >  1:807183/1‑140 (MQ=255)                                                                                                   aaCGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCggg                                    >  1:959521/1‑140 (MQ=255)                                                                                                    aCGTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCgggg                                   <  2:1295092/140‑1 (MQ=255)                                                                                                      gTTTTACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGgcg                                 <  2:205740/140‑1 (MQ=255)                                                                                                       ttttACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGgcgc                                <  1:240261/140‑1 (MQ=255)                                                                                                       ttttACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGgcgc                                <  1:357116/140‑1 (MQ=255)                                                                                                       ttttACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGgcgc                                <  1:1336772/140‑1 (MQ=255)                                                                                                        tttACCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACGCCGGCAAAGAGATGATCGTCGGTGTTGACGACCAGTGAATCTACGGCTTGCTCGGGGGGCa                               >  2:964667/1‑140 (MQ=255)                                                                                                           aCCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGt                            <  2:350561/140‑1 (MQ=255)                                                                                                           aCCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGt                            <  2:1485360/140‑1 (MQ=255)                                                                                                           aCCAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGt                            <  2:1440419/140‑1 (MQ=255)                                                                                                             cAATATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTgg                          <  2:176322/140‑1 (MQ=255)                                                                                                              aaTATCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGga                         <  1:279367/140‑1 (MQ=255)                                                                                                                  tCCTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCGGTCAATCTACGCCTGGCGCGGGGCGCAGGTGGAGAat                     >  1:706480/1‑140 (MQ=255)                                                                                                                    cTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGCTGACGACCAGTCAATCCACGGCTGGGGCGGGGCGCAGGGGGAGAatat                   >  2:633061/1‑140 (MQ=255)                                                                                                                    cTGGTGGACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAatat                   >  2:1073355/1‑140 (MQ=255)                                                                                                                      ggtggACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTc                 >  1:1112471/1‑140 (MQ=255)                                                                                                                       gtggACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGgcg                                 <  1:156936/123‑1 (MQ=255)                                                                                                                       gtggACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGgcg                                 >  2:156936/1‑123 (MQ=255)                                                                                                                          gACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGTCCAGTCAAGCTATGGCAGGCGCGGGACGCAAGTGGAGAATATTCAGCg             >  2:463984/1‑140 (MQ=255)                                                                                                                          gACGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTCAGCg             <  1:1241318/140‑1 (MQ=255)                                                                                                                           aCGAATTCCAGGATGTCAACAATATTCAGGACGCGTGCATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTCAGCGt            <  1:402406/140‑1 (MQ=255)                                                                                                                           aCGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTCAGCGt            <  1:848439/140‑1 (MQ=255)                                                                                                                           aCGAATTCCAGGATGCCAACAACATTCAGTACGCGAGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTCAGCGt            <  2:651464/140‑1 (MQ=255)                                                                                                                           aCGAATTCCAGGATGACAACAACATGCAGAACGCGTGCATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTCAGCGt            <  1:656489/140‑1 (MQ=255)                                                                                                                            cGAATTCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGGGGAGAATATTCAGCGtt           >  2:1239994/1‑140 (MQ=255)                                                                                                                                ttCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTCAGCGTTTCCt       <  2:1411279/140‑1 (MQ=255)                                                                                                                                 tCCAGGATGCCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTCAGCGTTTCCtt      >  2:1135667/1‑140 (MQ=255)                                                                                                                                  ccAGGATGCCAACAACATTCAGTACGCGTGGATCCGCGTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTCAGCGTTTCCTTa     <  1:633061/140‑1 (MQ=255)                                                                                                                                  ccAGGATGCCAACAACATTCAGGACGCGTAGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTCAGCGTTTCCTTa     <  1:669529/140‑1 (MQ=255)                                                                                                                                     ggATGCCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTCAGCGTTTCCTTAATg  >  1:899857/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        AGCGTGTGACCGCGCGGGCCTGGTGGACTTCGCCGAGCTGCTGCTGCGCGCTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTCAGCGTTTCCTTAATG  >  NC_000913/3998519‑3998790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑