| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,143,734 | A→G | V37V (GTA→GTG) | frwB → | putative PTS enzyme IIB component FrwB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,143,734 | 0 | A | G | 98.4% | 212.4 / NA | 63 | V37V (GTA→GTG) | frwB | putative PTS enzyme IIB component FrwB |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (38/24); minor base T (0/1); total (38/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.97e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGAAATTAATTAATTGAGGAACGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTAGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACCAAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGTGCGCGTC > NC_000913/4143597‑4143869 | tGAAATTAATTAATTGAGGAACGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGTGGCGCTGGTAAGGGGCGAGAAAGCGGAAGGCCGGGAAGTGAAAGTGGa > 2:737444/1‑140 (MQ=255) ttaattaattGAGGAACGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGATGGGAAGTGAAAGTGGAAAccc > 1:566896/1‑140 (MQ=255) gAACGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAGAGCGAAAGGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCAATTgg > 2:1225038/1‑140 (MQ=255) cGAGCCATGACGAAATTTATTGAAGCAACTGCATGTCTTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCAATTGGTCt < 1:737444/140‑1 (MQ=255) cGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCaa < 1:1268740/133‑1 (MQ=255) cGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCaa > 2:1268740/1‑133 (MQ=255) aGCCATGACTAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTgg > 2:802041/1‑140 (MQ=255) aGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTgg > 2:816538/1‑140 (MQ=255) aGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCCGGGTAGTTAAAGTGGAAACCCAGGGGTCAATTGGTCTgg > 2:636553/1‑140 (MQ=255) ccATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTGGaa < 2:1329921/140‑1 (MQ=255) aCGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGGAAGTGGAAACCGAGGGGTCAAATGGTCTGGAAAATGa > 1:472764/1‑140 (MQ=255) aCCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTGGGAACCCAGGAGTCAATTGGTCTGGAAAATGATCTGACTGCGGAAGAtgt > 2:475801/1‑140 (MQ=255) gCATGTCCTTCAGGTGTCGCCCTTACCTATAGGGCAGCATAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGc < 2:566896/140‑1 (MQ=255) tGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAg > 1:1527156/1‑140 (MQ=255) tGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAg < 1:802041/140‑1 (MQ=255) tCCTTCAGGTGTTGCTCATACCTATATGGCAGCAGAGCCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAgcg < 1:606959/140‑1 (MQ=255) tCCTTCAGGTGTTGCGCATACCAATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAgcg < 1:563203/140‑1 (MQ=255) tCAGGTGTTGCCCATCCCTATATGGAAGCCGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGa < 1:475910/140‑1 (MQ=255) ttGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGCTGTGGCGAGCGCTGATATGGTg > 1:416448/1‑140 (MQ=255) ttGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGa > 1:252278/1‑133 (MQ=255) ttGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGa < 2:252278/133‑1 (MQ=255) tGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGc > 1:1422812/1‑123 (MQ=255) tGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGc < 2:1422812/123‑1 (MQ=255) gCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGAt > 2:1142237/1‑140 (MQ=255) gCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGAt > 1:225687/1‑140 (MQ=255) gCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGAt > 1:212376/1‑140 (MQ=255) gCCCATACCTATATGGCAGCAGAGGCGCTAGAAAGTGACGCGAAAGCGAAAGGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGAt < 1:636553/140‑1 (MQ=255) cccATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGAtt < 2:225687/140‑1 (MQ=255) aTACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAGAGCGAAAGGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTg < 1:1225038/140‑1 (MQ=255) aCCTATATGGCAGCATAGGCGCGGGTAAGAGCCGCGACAGCGAAAGGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGAc < 1:475801/140‑1 (MQ=255) tatGGCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACCAAAg > 2:1357279/1‑140 (MQ=255) ggCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACCAAAGata > 1:1119780/1‑140 (MQ=255) cagcagAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGa > 2:33488/1‑129 (MQ=255) cagcagAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGa < 1:33488/129‑1 (MQ=255) cagcagAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACCa > 2:261161/1‑132 (MQ=255) cagcagAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACCa < 1:261161/132‑1 (MQ=255) cagcagAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACCAAAGATATc > 2:1159653/1‑140 (MQ=255) cagcagAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACCAAAGATATc > 1:1104318/1‑140 (MQ=255) cagcagAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACAGCGGAAGATGTGCCGAACGCTGATACGGTGCTTCTGACCAAAGATATc > 2:488097/1‑140 (MQ=255) agcagAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACCAAAGATATCg > 2:1069417/1‑140 (MQ=255) agcagAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTCGCGAGCGGTGAGATGGTGATTATGACCAAAGATATCg > 1:655704/1‑140 (MQ=255) gcagAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGAtt > 1:941394/1‑123 (MQ=255) gcagAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGAtt < 2:941394/123‑1 (MQ=255) gcagAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACCAAAGATATCgg > 2:996411/1‑140 (MQ=255) gcagAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACCAAAGATATCgg > 2:1517189/1‑140 (MQ=255) gcagAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACCAAAGATATCgg > 2:1157125/1‑140 (MQ=255) gcagAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACCAAAGATATCgg > 1:966154/1‑140 (MQ=255) gcagAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACCAAAGATATCgg > 1:276799/1‑140 (MQ=255) gcagAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGACGAGCGCAGATTTGTTGATTCTGACCAAAGATATCgg > 1:627420/1‑140 (MQ=255) cagAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACCAAAGATATCGGc > 2:316660/1‑140 (MQ=255) agagGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACCAAAGATATCGGCa > 1:225180/1‑140 (MQ=255) gagGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACCAAAGATATCGGCAt > 2:1423852/1‑140 (MQ=255) gagGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACCAAAGATATCGGCAt < 2:276799/140‑1 (MQ=255) tGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACCAAAGATATCGGCATCAAGTTTGAAGAGc > 2:777521/1‑140 (MQ=255) cgcgAAAGCGAAAGGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATGCTGACCAAAGAAATCGGCATCGAGTTTGAAGAGCGtt > 1:735289/1‑140 (MQ=255) gaaagcgacagGCTGGGTAGTGGAAGTGGAAACCCTGGGTTCAATTGGGCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACCAAAGATATCGGCATCAAGTTTGAAGAGCGTTTTg < 2:735289/140‑1 (MQ=255) aaagcgaaagGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATAAGGTGATTCAGACAAAACATATCGGCTTCAAGTTTGAAGAGCGTTTTGc > 2:500140/1‑140 (MQ=255) gagcgaaagGCTGGGCAGTGAACGTTGAATCCCAGGGATCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACCAAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCt < 1:560255/139‑1 (MQ=255) gaaagGCTGGGAAGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACCAAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCa < 1:1142237/140‑1 (MQ=255) gggAAGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACGAAAGATATAGGCATCAGGTTTGAAGGGCGTGTTGCTGGCAAGACCATc > 2:560255/1‑140 (MQ=255) aaGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACCAAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGTg < 1:316660/140‑1 (MQ=255) aaGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACCAAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGTg < 1:816538/140‑1 (MQ=255) aaGTGAAAGTGGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACCAAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGTg < 1:1423852/140‑1 (MQ=255) aaGTGGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACCAAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGTGCGCGTc < 2:225180/140‑1 (MQ=255) | TGAAATTAATTAATTGAGGAACGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTAGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACCAAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGTGCGCGTC > NC_000913/4143597‑4143869 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |