BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,241,977	A→G	V93A (GTC→GCC)	xylE ←	D‑xylose:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,241,977	0	A	G	96.8%	320.4 / NA	95	V93A (GTC→GCC)	xylE	D‑xylose:H(+) symporter
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (56/36); minor base C (0/3); total (56/39)
Fisher's exact test for biased strand distribution p-value = 6.60e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTA  >  NC_000913/4241842‑4242112

aTACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCGAGGCAGACCCTGCACCAGAAACAAAAAACAGGGcagc                                                                                                                                     >  1:680092/1‑140 (MQ=255)

 tACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGgcagca                                                                                                                                    >  1:310114/1‑140 (MQ=255)

 tACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGgcagca                                                                                                                                    >  2:100665/1‑140 (MQ=255)

 tACCGCCAATAATGCGAGAAATAACAAATTCGGGGACATGACCTGCCAAATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGgcagca                                                                                                                                    <  2:632709/140‑1 (MQ=255)

  aCCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGcagcag                                                                                                                                   <  1:601981/140‑1 (MQ=255)

             tGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGt                                                                                                                        >  2:232035/1‑140 (MQ=255)

                   aaataaCAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGGAATAAAAAACACGGCAGCAGCAATCTTAAGTGAATCa                                                                                                                  >  1:596897/1‑140 (MQ=255)

                            aTTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCg                                                                                                         >  1:809138/1‑140 (MQ=255)

                              tCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAGTCTTAAGTGAATCACGACGACCGaa                                                                                                       >  1:589496/1‑140 (MQ=255)

                              tCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGaa                                                                                                       <  2:949187/140‑1 (MQ=255)

                              tCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGaa                                                                                                       >  2:1096218/1‑140 (MQ=255)

                               ccGGGACATAACCTGTCAGATATACTGGCTCAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAg                                                                                                      <  1:611983/140‑1 (MQ=255)

                                cGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGc                                                                                                     <  2:1455256/140‑1 (MQ=255)

                                  ggACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCgg                                                                                                   >  1:1482288/1‑140 (MQ=255)

                                   gACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGt                                                                                                  <  2:63136/140‑1 (MQ=255)

                                   gACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGt                                                                                                  <  1:232035/140‑1 (MQ=255)

                                       tAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACt                                                                                              <  1:100665/140‑1 (MQ=255)

                                       tAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACt                                                                                              <  2:325365/140‑1 (MQ=255)

                                        aaCCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTg                                                                                             >  2:1330833/1‑140 (MQ=255)

                                        aaCCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTg                                                                                             >  1:816108/1‑140 (MQ=255)

                                         aCCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGc                                                                                            <  2:1180945/140‑1 (MQ=255)

                                          ccTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCa                                                                                           <  1:1096218/140‑1 (MQ=255)

                                              ccAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATaa                                                                                       >  1:1442039/1‑140 (MQ=255)

                                               cAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGc                                                                                                     >  2:259937/1‑125 (MQ=255)

                                               cAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGc                                                                                                     <  1:259937/125‑1 (MQ=255)

                                               cAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAac                                                                                      >  1:1485344/1‑140 (MQ=255)

                                               cAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAac                                                                                      >  2:938446/1‑140 (MQ=255)

                                                aGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTCAAGGGAAGCACGACGAACGAAACGGTTACTGCAATAacc                                                                                     >  2:504449/1‑140 (MQ=255)

                                                          gCACAGGGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCa                                                                           >  1:201437/1‑140 (MQ=255)

                                                           cacaGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGCCCGAAGCGGTTACTGCAATAACCCCCGAGGGCAc                                                                          <  1:853306/140‑1 (MQ=255)

                                                           cacaGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCAc                                                                          <  1:1155251/140‑1 (MQ=255)

                                                           cacaGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCAc                                                                          <  1:1174763/140‑1 (MQ=255)

                                                            acaGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCAcc                                                                         >  1:1209932/1‑140 (MQ=255)

                                                               gtgtTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCAccgcc                                                                      >  2:1103601/1‑140 (MQ=255)

                                                               gtgtTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCAccgcc                                                                      >  2:79029/1‑140 (MQ=255)

                                                               gtgtTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCAccgcc                                                                      <  1:938446/140‑1 (MQ=255)

                                                                tgttgtCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCAccgccg                                                                     <  2:1341954/140‑1 (MQ=255)

                                                                tgttgtCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCAccgccg                                                                     >  2:1153437/1‑140 (MQ=255)

                                                                tgttgtCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCAccgccg                                                                     >  2:303717/1‑140 (MQ=255)

                                                                tgttgtCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCAccgccg                                                                     >  1:1151536/1‑140 (MQ=255)

                                                                  ttgtCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCgat                                                                   >  1:64731/1‑140 (MQ=255)

                                                                    gtCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCAGGACGACCGGAGCGGTTGCTGCAATAGCCACCGAGGGCACCGCCgatga                                                                 >  2:539086/1‑140 (MQ=255)

                                                                      ccGGGTTTATAGAGGTATAACCAAGTTCTGGACAGTCAGTACCTACCCGAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGCCCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGatgatg                                                               <  2:554583/140‑1 (MQ=255)

                                                                      ccGGGTTTATAGAGGTACACCCGAGTTCTGGTCAGACAGAACCTACACTAGAAATAAAAAACAGGGCACAAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATACCCACCGAGGGCACCGCCGatgatg                                                               <  2:629900/140‑1 (MQ=255)

                                                                      ccGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTCCTGCAATACCCACCGAGGGCACCGCCGatgatg                                                               <  2:831946/140‑1 (MQ=255)

                                                                      ccGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGatgatg                                                               <  1:1103601/140‑1 (MQ=255)

                                                                      ccGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGatgatg                                                               <  1:79029/140‑1 (MQ=255)

                                                                        gggTTTATAGAGGAAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGCCAGCAGCAATCTTAAGGGAATCCCGACGCCCGAAGCGGTTACTGCAATACCCACCGAGGGCACCGCCGATGATGCa                                                             <  1:1544401/140‑1 (MQ=255)

                                                                        gggTTTAGAGAGCAAAAAGCAAGCTTTGGCCAGTCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCa                                                             <  2:1442039/140‑1 (MQ=255)

                                                                          gTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAAc                                                           >  2:1245285/1‑140 (MQ=255)

                                                                          gTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAAc                                                           >  2:1518325/1‑140 (MQ=255)

                                                                          gTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAAc                                                           >  2:1032303/1‑140 (MQ=255)

                                                                          gTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAAc                                                           >  2:361229/1‑140 (MQ=255)

                                                                          gTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAAc                                                           >  1:1313604/1‑140 (MQ=255)

                                                                          gTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGATACGGCAACAACCACCAAGGGGCCCGCCGACGATGCAAc                                                           >  2:742936/1‑140 (MQ=255)

                                                                               taGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATc                                                      <  2:809138/140‑1 (MQ=255)

                                                                                agagTTAAAACCAGTTTCTGGCGAGGCGGACCTTACACAGGAAATAAAAAACAGGCCACTAAAAGCCTTAATTGAACCGCGACGACCGAAGCGGTTGCTGCAATACCCTCCGAGGGCACCGCCGATGATGCAACCAATCa                                                     <  2:310114/140‑1 (MQ=255)

                                                                                    gTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCATCGCCGATGATGCAACCAATCAGAgc                                                 >  1:648519/1‑140 (MQ=255)

                                                                                    gTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCgatga                                                                 <  1:27041/124‑1 (MQ=255)

                                                                                    gTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCgatga                                                                 >  2:27041/1‑124 (MQ=255)

                                                                                    gTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGatgatg                                                               >  2:916566/1‑126 (MQ=255)

                                                                                    gTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGatgatg                                                               <  1:916566/126‑1 (MQ=255)

                                                                                    gTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAgc                                                 >  2:278689/1‑140 (MQ=255)

                                                                                    gTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAgc                                                 >  1:1150441/1‑140 (MQ=255)

                                                                                    gTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAgc                                                 >  1:1129512/1‑140 (MQ=255)

                                                                                    gTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAgc                                                 >  1:1019136/1‑140 (MQ=255)

                                                                                    gTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCCCCGATGATGCAACCAATCAGAgc                                                 >  1:34546/1‑140 (MQ=255)

                                                                                    gTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTCACTGCAATAACCACCGAGGGCACCGACGATTATGCAACCAATCAGAgc                                                 >  2:667306/1‑140 (MQ=255)

                                                                                     tAAAACCAAGTTCTGCCAAGGCTGATCCTACACCAGACATAAAAAACAGGCCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAgcg                                                <  1:414125/140‑1 (MQ=255)

                                                                                       aaaCCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCt                                              >  1:1233165/1‑140 (MQ=255)

                                                                                        aaCCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTCCAATAACCTCCGGGGGGACCGCCGAGGATGCAACCAATCAGAGCGCTg                                             >  2:545253/1‑140 (MQ=255)

                                                                                          ccAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGc                                           <  2:1313604/140‑1 (MQ=255)

                                                                                             aGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCAc                                        >  1:189475/1‑140 (MQ=255)

                                                                                                 cTGGCCAGGCAGAACCTACACCAGAAATACAAAACAGAGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCaa                                    <  1:539086/140‑1 (MQ=255)

                                                                                                     ccAGGCAGAACCTACTCCAGAATTACAAATCAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAAcc                                <  1:504449/140‑1 (MQ=255)

                                                                                                     ccAGGCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGTAAAAcc                                <  2:909077/140‑1 (MQ=255)

                                                                                                        ggCAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGTCACCGCCGCTGAGGCAACCAATCAGGGCGCGGGCCACGCAAAACCCTa                             >  2:495217/1‑140 (MQ=255)

                                                                                                          cAGACCCTACACCAGTAATAAATAACAGGCCAGCAGCTATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAAc                           <  1:495217/140‑1 (MQ=255)

                                                                                                          cAGAACCTACACCAGATAAAAACAACAGGGCAGCACCAATTTTAAGTATATCCCGACGCCCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAAc                           <  2:680092/140‑1 (MQ=255)

                                                                                                          cAGAACCTACACCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAAc                           <  1:60389/140‑1 (MQ=255)

                                                                                                                  acacCAGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGtt                   >  2:1146629/1‑140 (MQ=255)

                                                                                                                       aGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAg              >  2:235242/1‑140 (MQ=255)

                                                                                                                       aGAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAg              >  1:1457812/1‑140 (MQ=255)

                                                                                                                        gAAATAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCGACGGAAGACCCTAGCAGGGAGTTGGCAGc             >  1:404680/1‑140 (MQ=255)

                                                                                                                         aaaTAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGGGCTGGCCGCGCAAAACCCTAAGAGGGAGTTGGCAGCg            >  1:509481/1‑140 (MQ=255)

                                                                                                                         aaaTAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTa                             <  2:1393778/123‑1 (MQ=255)

                                                                                                                         aaaTAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTa                             >  1:1393778/1‑123 (MQ=255)

                                                                                                                         aaaTAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCg            >  1:1304790/1‑140 (MQ=255)

                                                                                                                         aaaTAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCg            >  1:103647/1‑140 (MQ=255)

                                                                                                                          aaTAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAACACCCTAACAGGGAGTTGGCAGCgg           >  2:1022326/1‑140 (MQ=255)

                                                                                                                          aaTAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCgg           >  2:858017/1‑140 (MQ=255)

                                                                                                                           aTAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGa          <  1:235242/140‑1 (MQ=255)

                                                                                                                           aTAAAAAACAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGa          <  2:939620/140‑1 (MQ=255)

                                                                                                                             aaaaaaCAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGAtt        >  1:97846/1‑140 (MQ=255)

                                                                                                                                aaaCAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCCCCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAc     <  2:1485344/140‑1 (MQ=255)

                                                                                                                                aaaCAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGTAGTTGGCAGCGGATTCAc     >  1:1028195/1‑140 (MQ=255)

                                                                                                                                aaaCAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAc     <  1:303717/140‑1 (MQ=255)

                                                                                                                                aaaCAGGGCAGCAACAATCTTAGGTGAATCCCGACGCCCGAAGCGGTTACTGCAATAACCACCAAGGCCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCAc     <  2:1209932/140‑1 (MQ=255)

                                                                                                                                  aCAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACtt   >  1:961553/1‑140 (MQ=255)

                                                                                                                                   cAGGGCAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCGTAATAGCGAGTTGTCAGCGGATTCACTTa  >  1:721735/1‑140 (MQ=255)

ATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTA  >  NC_000913/4241842‑4242112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`