Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,283,282	A→G	F541L (TTT→CTT)	actP ←	acetate/glycolate:cation symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,283,282	0	A	G	100.0%	328.8 / NA	93	F541L (TTT→CTT)	actP	acetate/glycolate:cation symporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (51/42);  total (51/42)

GCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATAC  >  NC_000913/4283150‑4283418                                                                                                                                     |                                                                                                                                         gCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTc                                                                                                                                   <  2:50656/140‑1 (MQ=255)  cTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCt                                                                                                                                  >  1:168650/1‑140 (MQ=255)  cTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCt                                                                                                                                  >  1:204536/1‑140 (MQ=255)  cTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGAGTAATGCGCGCGGCCATGCTCAACGCCAAGGCCGGTCt                                                                                                                                  >  1:524767/1‑140 (MQ=255)     aTTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTggg                                                                                                                               <  1:950295/140‑1 (MQ=255)       tttttAATGAGTTTATTTGTTTCATATTATGAGAAAAGGCGATGCATTCGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAg                                                                                                                             <  2:524767/140‑1 (MQ=255)            aaTGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGAt                                                                                                                        <  2:204536/140‑1 (MQ=255)                   ttATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTgc                                                                                                                 <  1:851698/140‑1 (MQ=255)                   ttATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTgc                                                                                                                 <  1:1097122/140‑1 (MQ=255)                      tttGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTgcgcg                                                                                                              <  1:770630/140‑1 (MQ=255)                      tttGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTgcgcg                                                                                                              <  2:1194054/140‑1 (MQ=255)                           ttAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGaa                                                                                                         <  2:226334/140‑1 (MQ=255)                            tAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGTTCTCGGGGCGGATGAACTGCGCGCGGAAc                                                                                                        >  1:648201/1‑140 (MQ=255)                            tAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTgcgcg                                                                                                              >  1:1469108/1‑134 (MQ=255)                            tAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTgcgcg                                                                                                              <  2:1469108/134‑1 (MQ=255)                            tAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAAc                                                                                                        >  1:933738/1‑140 (MQ=255)                            tAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAAc                                                                                                        >  1:1050596/1‑140 (MQ=255)                                    tGGGAAAAGGTGATGCATTTGGGAGAGGATGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTtcacg                                                                                                >  1:830746/1‑140 (MQ=255)                                    tGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTtcacg                                                                                                >  2:1404510/1‑140 (MQ=255)                                    tGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTtcacg                                                                                                >  2:863927/1‑140 (MQ=255)                                    tGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTtcacg                                                                                                >  2:1518731/1‑140 (MQ=255)                                         aaaGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTtcacgctcac                                                                                           >  1:800128/1‑140 (MQ=255)                                           aGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACACCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTtcacgctcacgc                                                                                         <  1:1185604/140‑1 (MQ=255)                                            ggTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCAcgcg                                                                                        <  1:1226324/140‑1 (MQ=255)                                              tGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCAcgcgcg                                                                                      >  2:219218/1‑140 (MQ=255)                                                aTGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGcc                                                                                    >  2:831762/1‑140 (MQ=255)                                                aTGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGcc                                                                                    >  1:220499/1‑140 (MQ=255)                                                aTGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGcc                                                                                    >  1:910212/1‑140 (MQ=255)                                                 tGCATTTGGGAGAGGAAGAGTATTCCCCGGACATAGGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCt                                                                                   <  2:712694/140‑1 (MQ=255)                                                      ttGGGAGAGGAAGAGAATTCCCGGGGCAGTCGACCGGTGAAGGGGAGAGA‑TAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCt                                                                             <  1:704202/140‑1 (MQ=255)                                                       tGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGA‑TAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGGGGATAAACAGCGCGTGGAACAGTTCGCGCTAACGCGCGCGTTCCGCTg                                                                            >  2:704202/1‑140 (MQ=255)                                                             agGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTa                                                                       >  2:902886/1‑140 (MQ=255)                                                             agGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTa                                                                       >  1:1100273/1‑140 (MQ=255)                                                             agGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTACACTCTGACGCGCACCTTCCGCTGAGTTa                                                                       >  1:645534/1‑140 (MQ=255)                                                               gAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATc                                                                     >  2:204469/1‑140 (MQ=255)                                                               gAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATc                                                                     >  1:104453/1‑140 (MQ=255)                                                                aaGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCg                                                                    >  2:1477074/1‑140 (MQ=255)                                                                    gTATTCCCCGGTCAGACTACCGTGGAAGGGGTGAGTTTATTGCGCGCGCCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTg                                                                <  2:478357/140‑1 (MQ=255)                                                                       ttCCCCGGTCAGACGCCCGGGGGAGGGGATAGATTAACGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCg                                                             <  1:451273/140‑1 (MQ=255)                                                                       ttCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCg                                                             <  2:1050596/140‑1 (MQ=255)                                                                       ttCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCg                                                             >  2:37597/1‑140 (MQ=255)                                                                       ttCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGAGGAACAGTTCACGCTCACGCGCGCCATCCGGTGAGTTATCGGTTGCCg                                                             >  2:447126/1‑140 (MQ=255)                                                                         ccccGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCgag                                                           >  1:1031797/1‑140 (MQ=255)                                                                         ccccGGTCAGACGACCGAGGAACGGGAGAGATTAACGCGTGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCgag                                                           <  2:645534/140‑1 (MQ=255)                                                                          cccGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCgaga                                                          >  1:1053504/1‑140 (MQ=255)                                                                           ccGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGagaa                                                         >  1:1243560/1‑140 (MQ=255)                                                                             ggTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGagaaga                                                       >  1:1348438/1‑140 (MQ=255)                                                                               tCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAAc                                                     >  1:1536570/1‑140 (MQ=255)                                                                                       ccGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGcc                                             <  1:13300/140‑1 (MQ=255)                                                                                             aaGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGaa                                       >  1:1178867/1‑140 (MQ=255)                                                                                              aGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAAt                                      <  1:863927/140‑1 (MQ=255)                                                                                              aGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAAt                                      <  1:204469/140‑1 (MQ=255)                                                                                                gggAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGc                                    <  2:9114/140‑1 (MQ=255)                                                                                                 ggAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGcc                                   >  1:1069926/1‑140 (MQ=255)                                                                                                 ggAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGcc                                   >  2:794420/1‑140 (MQ=255)                                                                                                 ggAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCATCCGCTGATTTATCGGGTGCCGAGACGAACTAGATGCCGAGGAATGcc                                   >  2:429349/1‑140 (MQ=255)                                                                                                  gagagaTTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCa                                  <  1:353837/140‑1 (MQ=255)                                                                                                  gagagaTTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCa                                  <  2:168650/140‑1 (MQ=255)                                                                                                  gagagaTTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCa                                  <  1:1318601/140‑1 (MQ=255)                                                                                                  gagagaTTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCa                                  <  1:1404510/140‑1 (MQ=255)                                                                                                   agagaTTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCAc                                 <  2:1348438/140‑1 (MQ=255)                                                                                                     agaTTAATGCGCGCGGCGTTGCCCAACGCCACGGCGGGTCTGGGAGCGTATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACgg                               <  2:412750/140‑1 (MQ=255)                                                                                                     agaTTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACgg                               <  2:1178867/140‑1 (MQ=255)                                                                                                          aaTGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGTCGAGAAGAACCTGACGCCGAGTAATGCCACGGTGATa                          >  1:647608/1‑140 (MQ=255)                                                                                                          aaTGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATa                          <  1:794420/140‑1 (MQ=255)                                                                                                            tGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCTAGAAGAACCAGCTGCCGAGGAATGCCACGGTGATaga                        >  1:695487/1‑140 (MQ=255)                                                                                                            tGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATaga                        >  2:167572/1‑140 (MQ=255)                                                                                                               gcgcgGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGaa                     <  1:1477074/140‑1 (MQ=255)                                                                                                                cgcgGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGGCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAAc                    >  2:210752/1‑140 (MQ=255)                                                                                                                cgcgGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAAc                    >  1:1140131/1‑140 (MQ=255)                                                                                                                cgcgGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCCGTTGCCGAAAAGAACCGGATGCCGAGGAATGCCACGGTGATAGAGAAc                    >  2:478777/1‑140 (MQ=255)                                                                                                                 gcgGCCTTGCTCAACGCCAAGGCCGGTCTGGGGGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGAGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGCAATGCCACGGTGATAGAGAACa                   >  2:1459576/1‑140 (MQ=255)                                                                                                                 gcgGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACa                   >  2:991072/1‑140 (MQ=255)                                                                                                                 gcgGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGATTGCCGAGAAGATCGATCTGCCGAGTAATGGCACGGTGAAAGAGAACa                   >  2:740432/1‑140 (MQ=255)                                                                                                                    gCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAgcg                <  1:1164308/140‑1 (MQ=255)                                                                                                                    gCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAgcg                <  2:1140131/140‑1 (MQ=255)                                                                                                                    gCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAgcg                <  1:37597/140‑1 (MQ=255)                                                                                                                     ccTTGCTCAACGCCAAGGCCGGGCTGGGAGCGGATCAACTGCGCGCGGTACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAgcgc               <  2:648201/140‑1 (MQ=255)                                                                                                                      cTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGcc              <  1:1406003/140‑1 (MQ=255)                                                                                                                      cTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGcc              <  1:219218/140‑1 (MQ=255)                                                                                                                      cTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGcc              <  2:1031797/140‑1 (MQ=255)                                                                                                                       ttGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCg             <  1:1453560/140‑1 (MQ=255)                                                                                                                        tGCTCAACGCCAAGGGCGGTCTGGGAGCGAATAAACTACGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCgg            <  1:740432/140‑1 (MQ=255)                                                                                                                         gCTCAACGCCAAGGCCGGTCTGGGATCGGATAAACTGCGCGCGGCACAGCTCACGCTCACGCCCGCCTTCCGCTGAGGCATCGGTTGCCGAGATGAACCAGGTGCCGAGGAATGCCACGGTGATAGGGAACAGCGCCggg           >  2:1354465/1‑140 (MQ=255)                                                                                                                             aaCGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTAtt       >  2:1315514/1‑140 (MQ=255)                                                                                                                               cGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGCATGCCACGGTGATAGAGAACAGCGCCGGGTATTCa     >  2:1109520/1‑140 (MQ=255)                                                                                                                               cGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCgg            <  2:1292139/133‑1 (MQ=255)                                                                                                                               cGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCgg            >  1:1292139/1‑133 (MQ=255)                                                                                                                               cGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCa     >  2:1352721/1‑140 (MQ=255)                                                                                                                               cGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCa     >  2:7909/1‑140 (MQ=255)                                                                                                                               cGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCa     >  2:1170730/1‑140 (MQ=255)                                                                                                                               cGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCa     <  2:822907/140‑1 (MQ=255)                                                                                                                               cGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCa     >  1:1004675/1‑140 (MQ=255)                                                                                                                               cGCCAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCa     >  2:964628/1‑140 (MQ=255)                                                                                                                                 ccAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATa   >  1:791804/1‑140 (MQ=255)                                                                                                                                  cAAGGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATAc  <  2:791804/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                         GCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATAC  >  NC_000913/4283150‑4283418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑