Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	539,690	C→T	P182S (CCG→TCG)	allB →	allantoinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	539,690	0	C	T	98.9%	305.2 / NA	91	P182S (CCG→TCG)	allB	allantoinase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (43/47);  total (44/47)
Fisher's exact test for biased strand distribution p-value = 4.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTAACCGCTCATGACTATGTGGCTTCGCGTCCGGTATTTACCGAAGTGGAAGCAATTCGCCGCGT  >  NC_000913/539555‑539826                                                                                                                                        |                                                                                                                                         cTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGt                                                                                                                                      >  1:357062/1‑140 (MQ=255)     aTGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTg                                                                                                                                  >  1:1458383/1‑140 (MQ=255)        aaGTGGGCTTTGTCGGACTCAAATGCTTCGGTGAGCCCTTTGGCGATCCCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTg                                                                                                                               <  2:736269/140‑1 (MQ=255)          gTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCgg                                                                                                                                       <  2:1110818/130‑1 (MQ=255)          gTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGAATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCgg                                                                                                                                       >  1:1110818/1‑130 (MQ=255)              gCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCgg                                                                                                                                       >  1:1490245/1‑126 (MQ=255)              gCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCgg                                                                                                                                       <  2:1490245/126‑1 (MQ=255)              gCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGc                                                                                                                         >  1:1192864/1‑140 (MQ=255)               cGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCg                                                                                                                        >  1:1458146/1‑140 (MQ=255)               cGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGCGCACAGAAGCTGGGCGAACTGGGTCAGGCGGTGCTGGTGCACTGCg                                                                                                                        >  2:1164590/1‑140 (MQ=255)                  tGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGaaa                                                                                                                     <  1:1349983/140‑1 (MQ=255)                  tGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGaaa                                                                                                                     >  2:1053969/1‑140 (MQ=255)                  tGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGaaa                                                                                                                     <  1:248690/140‑1 (MQ=255)                   gTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGaaaa                                                                                                                    >  1:1389375/1‑140 (MQ=255)                   gTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTAAGCCGGTGCTGGTGCACTGCGaaaa                                                                                                                    >  1:691083/1‑140 (MQ=255)                    tCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAAc                                                                                                                   <  1:302457/140‑1 (MQ=255)                       gCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAAcgcg                                                                                                                <  1:1348605/140‑1 (MQ=255)                       gCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAAcgcg                                                                                                                <  1:9193/140‑1 (MQ=255)                       gCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAAcgcg                                                                                                                <  1:341394/140‑1 (MQ=255)                       gCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGGGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAAcgcg                                                                                                                >  1:1052417/1‑140 (MQ=255)                            aaaTGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGAt                                                                                                           <  1:1053969/140‑1 (MQ=255)                              aTGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTCGTGCACTGCGAAAACGCGCTGAttt                                                                                                         <  2:1052417/140‑1 (MQ=255)                                  ttCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGa                                                                                                     >  1:360332/1‑140 (MQ=255)                                   tCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGAc                                                                                                    <  1:197530/140‑1 (MQ=255)                                    cGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACg                                                                                                   >  1:836059/1‑140 (MQ=255)                                     gTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACAGGGTCAGTCGGTTCTGATGCACTGCAAAAACACGCTGATATGTGACGa                                                                                                  >  2:708765/1‑140 (MQ=255)                                      ttGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGaa                                                                                                 <  2:357062/140‑1 (MQ=255)                                       tGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACCGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAAc                                                                                                >  1:146364/1‑140 (MQ=255)                                           aCCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGGGACGAACTggg                                                                                            >  2:230018/1‑140 (MQ=255)                                                tgGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCgaag                                                                                       >  2:1064117/1‑140 (MQ=255)                                                   cGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGaagaag                                                                                    <  2:1120717/140‑1 (MQ=255)                                                    gATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCTCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAgaagc                                                                                   <  1:1164590/140‑1 (MQ=255)                                                     aTCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAgaagcg                                                                                  >  1:232442/1‑140 (MQ=255)                                                     aTCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAgaagcg                                                                                  <  2:1407393/140‑1 (MQ=255)                                                      tCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAgaagcga                                                                                 <  2:1192864/140‑1 (MQ=255)                                                      tCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAgaagcga                                                                                 <  2:1351806/140‑1 (MQ=255)                                                      tCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAgaagcga                                                                                 <  2:972895/140‑1 (MQ=255)                                                         cgGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAgaagcgaagc                                                                              >  2:1147838/1‑140 (MQ=255)                                                            tATCGACAACGACTTGCGTGATGTACACGTCTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTg                                                                           <  1:672695/140‑1 (MQ=255)                                                              tCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGaa                                                                         <  1:1147838/140‑1 (MQ=255)                                                                 aCAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGt                                                                      >  2:178862/1‑140 (MQ=255)                                                                  cAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGTGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTc                                                                     >  1:255963/1‑140 (MQ=255)                                                                  cAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTc                                                                     >  2:1492602/1‑140 (MQ=255)                                                                      gACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGGGCTGATTTGTGAAGAAATGGGCGAAAAAGCGTAGCGTGAAGGTCGCGt                                                                 >  2:679515/1‑140 (MQ=255)                                                                      gACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGt                                                                 >  2:90667/1‑140 (MQ=255)                                                                      gACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGt                                                                 <  1:90667/140‑1 (MQ=255)                                                                        cTTCCGTGATGTAAATGACTGGCAGTTCTGCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTaa                                                               <  1:492389/140‑1 (MQ=255)                                                                        cTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTaa                                                               <  2:1458146/140‑1 (MQ=255)                                                                        cTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTaa                                                               <  1:343956/140‑1 (MQ=255)                                                                        cTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTaa                                                               >  2:188023/1‑140 (MQ=255)                                                                        cTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTaa                                                               <  1:848951/140‑1 (MQ=255)                                                                         ttCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTAAc                                                              <  1:230018/140‑1 (MQ=255)                                                                          tCCGTGATGTAAACGGCTGGCAGTTCTTCTAAGGTGCTCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTAAcc                                                             <  1:511048/140‑1 (MQ=255)                                                                          tCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTAAcc                                                             >  1:660857/1‑140 (MQ=255)                                                                           ccGTGATGTAGACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACAGAGAGAAGAAGCGAAGCCAGAAGGTCTCGTAACCg                                                            >  2:686779/1‑140 (MQ=255)                                                                                aTGTAAACGACTGGCAGTTCTTTAAAGGTGCTCAGCAGCCGGGCGAACAGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTAACCGCTCAt                                                       <  2:556915/140‑1 (MQ=255)                                                                                aTGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTAACCGCTCAt                                                       <  1:188023/140‑1 (MQ=255)                                                                                aTGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTAACCGCTCAt                                                       <  1:1099910/140‑1 (MQ=255)                                                                                 tGTAAACGACTGGCAG‑TCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTAACCGCTCATGa                                                     <  1:351767/140‑1 (MQ=255)                                                                                  gTAAACGACTGGCAGATCTTCACAGCTGCACAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTAACCGCTCATGa                                                     <  1:708765/140‑1 (MQ=255)                                                                                   tAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACTAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTAACCGCTCATGAc                                                    >  2:61850/1‑140 (MQ=255)                                                                                   tAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGTAGAAGCGTAGCGTGTAGGTCGTGTAACCGCTCATGAc                                                    >  1:756893/1‑140 (MQ=255)                                                                                           tGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGTAGCGTGAAGGTGGCATAACCGCTCATGACTATGTGGc                                            >  1:530810/1‑140 (MQ=255)                                                                                           tGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTAACCGCTCATGACTATGTGGc                                            >  1:227298/1‑140 (MQ=255)                                                                                             gCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTAACCGCTCAt                                                       <  1:1534039/127‑1 (MQ=255)                                                                                             gCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTAACCGCTCATGACTATGTGGCtt                                          >  2:294207/1‑140 (MQ=255)                                                                                             gCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTAACCGCTCATGACTATGTGGCtt                                          >  2:1104112/1‑140 (MQ=255)                                                                                             gCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTAACCGCTCATGACTATGTGGCtt                                          >  1:1192624/1‑140 (MQ=255)                                                                                             gCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTAACCGCTCATGACTATGTGGCtt                                          >  1:199575/1‑140 (MQ=255)                                                                                             gCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTAACCGCTCATGACTATGTGGCtt                                          <  1:1201990/140‑1 (MQ=255)                                                                                             gCAGGTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTAACCGCTCAt                                                       >  2:1534039/1‑127 (MQ=255)                                                                                                  tcttcAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGGAGGTCGCGTAACCGGTCATGACTATGTGGCTTCGCGt                                     >  1:603903/1‑140 (MQ=255)                                                                                                  tcttcAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTAACCGCTCATGACTATGTGGCTTCGCGt                                     <  2:925834/140‑1 (MQ=255)                                                                                                  tcttcAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTAACCGCTCATGACTATGTGGCTTCGCGt                                     >  2:1207314/1‑140 (MQ=255)                                                                                                   cttcAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTAACCGCTCATGACTATGTGGCTTCGCGTc                                    <  1:178862/140‑1 (MQ=255)                                                                                                   cttcAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTAACCGCTCATGACTATGTGGCTTCGCGTc                                    <  1:294207/140‑1 (MQ=255)                                                                                                   cttcAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTAACCGCTCATGACTATGTGGCTTCGCGTc                                    <  2:789510/140‑1 (MQ=255)                                                                                                     tcAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTAACCGCTCATGACTATGTGGCTTCGCGTCCg                                  <  2:1389375/140‑1 (MQ=255)                                                                                                           gTGCGCAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTAACCGCTCATGACTATGTGGCTTCGCGTCCGGTAttt                            >  1:1284746/1‑140 (MQ=255)                                                                                                              tgcAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTAACCGCTCATGACTATGTGGCTTCGCGTCCGGTATTTAcc                         >  1:76119/2‑140 (MQ=255)                                                                                                              cgcAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTAACCGCTCATGACTATGTGGCTTCGCGTCCGGTATTTAcc                         >  2:178566/1‑140 (MQ=255)                                                                                                              cgcAGAAGCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTAACCGCTCATGACTATGTGGCTTCGCGTCCGGTATTTAcc                         >  1:283853/1‑140 (MQ=255)                                                                                                                     gCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTAACCGCTCATGACTATGTGGCTTCGCGTCCGGTATTTACCGAAGTgg                  <  2:232442/140‑1 (MQ=255)                                                                                                                     gCTGGGCGAACTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTAACCGCTCATGACTATGTGGCTTCGCGTCCGGTATTTACCGAAGTgg                  >  1:259573/1‑140 (MQ=255)                                                                                                                             aaCTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTAACCGCTCATGACTATGTGGCTTCGCGTCCGGTATTTACCGAAGTGGAAGCAAtt          >  2:345057/1‑140 (MQ=255)                                                                                                                             aaCTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTAACCGCTCATGACTATGTGGCTTCGCGTCCGGTATTTACCGAAGTGGAAGCAAtt          >  2:782954/1‑140 (MQ=255)                                                                                                                               cTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGTGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTAACCGCTCATGACTATGTGGCTTCGCGTCCGGTATTTACCGAAGTGGAAGCAATTcg        <  2:255963/140‑1 (MQ=255)                                                                                                                               cTGGGTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTAACCGCTCATGACTATGTGGCTTCGCGTCCGGTATTTACCGAAGTGGAAGCAATTcg        >  1:314485/1‑140 (MQ=255)                                                                                                                                 gggTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTAACCGCTCATGACTATGTGGCTTCGCGTCCGGTATTTACCGAAGTGGAAGCAATTcgcc      <  2:199575/140‑1 (MQ=255)                                                                                                                                 gggTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTAACCGCTCATGACTATGTGGCTTCGCGTCCGGTATTTACCGAAGTGGAAGCAATTcgcc      <  2:360332/140‑1 (MQ=255)                                                                                                                                 gggTCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTAACCGCTCATGACTATGTGGCTTCGCGTCCGGTATTTACCGAAGTGGAAGCAATTcgcc      <  2:1064063/140‑1 (MQ=255)                                                                                                                                    tCAGTCGGTGCTGGTGCACTGCTAAGACGCGCTGATTAGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTAACCGCTCATGACTATGTGGCTTCGCGTCCGGTATTTACCGAAGTGGAAGCAATTCGCcgcg   <  1:686779/140‑1 (MQ=255)                                                                                                                                    tCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTAACCGCTCATGACTATGTGGCTTCGCGTCCGGTATTTACCGAAGTGGAAGCAATTCGCcgcg   >  1:1469771/1‑140 (MQ=255)                                                                                                                                    tCAGTCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTAACCGCTCATGACTATGTGGCTTCGCGTCCGGTATTTACCGAAGTGGAAGCAATTCGCcgcg   <  2:1284746/140‑1 (MQ=255)                                                                                                                                     cAGTCGGTGCTGGTGCACTGCGAAAACGCGCCGTTTTGTGACGAACTGTGCGAAGAAGCGAAGCGTGAAGGTCGCGTAACCGCTCATGACTATGTGGCTTCGCGTCCGGTATTTACCGAAGTGGAAGCAATTCGCCGCGt  <  2:691083/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         CTGGATGAAGTGGGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGACTTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGTCAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCGAAGCGTGAAGGTCGCGTAACCGCTCATGACTATGTGGCTTCGCGTCCGGTATTTACCGAAGTGGAAGCAATTCGCCGCGT  >  NC_000913/539555‑539826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑