Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,502,150	G→A	P428S (CCC→TCC)	insG ←	KpLE2 phage‑like element; IS4 putative transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,502,150	0	G	A	100.0%	297.8 / NA	90	P428S (CCC→TCC)	insG	KpLE2 phage‑like element; IS4 putative transposase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (42/48);  total (42/48)

AAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGTATACGTCCCGGTGAAGCGCCCTGCAATGTCATCAGCATTC  >  NC_000913/4502013‑4502284                                                                                                                                          |                                                                                                                                       aaaaaaGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGAcc                                                                                                                                      >  1:170054/1‑140 (MQ=255) aaaaaaGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGAcc                                                                                                                                      >  2:1172396/1‑140 (MQ=255) aaaaaaGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGAcc                                                                                                                                      >  1:1188833/1‑140 (MQ=255) aaaaaaGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTGAAGCAACTGACTCGCTTTTTTTCGGAGCTGTGGGGTATTTCCAGGAcc                                                                                                                                      >  2:487580/1‑140 (MQ=255)  aaaaaGCTCAGTATTCTCGTTGACAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACct                                                                                                                                     <  1:283533/140‑1 (MQ=255)   aaaaGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACctc                                                                                                                                    <  1:955630/140‑1 (MQ=255)   aaaaGCTCAGTATTCTCGTTGGAAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACctc                                                                                                                                    <  2:572910/140‑1 (MQ=255)           aGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTAc                                                                                                                            <  1:1451588/140‑1 (MQ=255)           aGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTAc                                                                                                                            <  2:1152431/140‑1 (MQ=255)           aGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTAc                                                                                                                            <  1:125958/140‑1 (MQ=255)                  tcGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGCATTACCCGGACCTCTCCTTTACCACtctc                                                                                                                     >  1:693412/1‑140 (MQ=255)                     ttGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCggg                                                                                                                  >  1:805124/1‑140 (MQ=255)                     ttGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCggg                                                                                                                  >  1:1359160/1‑140 (MQ=255)                      tGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGa                                                                                                                 <  2:939246/140‑1 (MQ=255)                       ggCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGaa                                                                                                                >  2:1086525/1‑140 (MQ=255)                       ggCAACCAGATAATTCAACAGTTGACGCTGAAACGAAGTAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGaa                                                                                                                <  1:658383/140‑1 (MQ=255)                         cAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAgg                                                                                                              >  2:763633/1‑140 (MQ=255)                          aaCCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGc                                                                                                             <  1:1251564/140‑1 (MQ=255)                          aaCCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGc                                                                                                             <  1:1211857/140‑1 (MQ=255)                          aaCCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGc                                                                                                             <  2:35008/140‑1 (MQ=255)                          aaCCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGc                                                                                                             <  2:1359160/140‑1 (MQ=255)                          aaCCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGc                                                                                                             <  1:1006995/140‑1 (MQ=255)                           gccAGATAATTTAACTGTTGATCCTCAAAAGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGcc                                                                                                            <  1:545330/139‑1 (MQ=255)                            ccAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGccc                                                                                                           >  1:994064/1‑140 (MQ=255)                                  aaTTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTccc                                                                                                     >  1:199689/1‑140 (MQ=255)                                       aaCAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGcc                                                                                                            >  2:47178/1‑128 (MQ=255)                                       aaCAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGcc                                                                                                            <  1:47178/128‑1 (MQ=255)                                       aaCAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCcttctt                                                                                                >  1:146970/1‑140 (MQ=255)                                       aaCAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTAATTCCAGGAACTCTCCTTTACCACTCTCGGGGAGGCCCTTTCCcttctc                                                                                                >  2:545330/1‑139 (MQ=255)                                              gATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTAACCCGCTCGGGAATGCCCTATCCCTTCCTGTCGGTa                                                                                         >  2:730890/1‑140 (MQ=255)                                              gATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGCATTTCCAGGACCTCTCCTTTACCACTCGCGGTAACGCCCTTTCCCTTCTTGTCGGTa                                                                                         >  1:493356/1‑140 (MQ=255)                                               aTGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTaa                                                                                        >  2:1259340/1‑140 (MQ=255)                                                  cTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAAttt                                                                                     <  2:1188833/140‑1 (MQ=255)                                                   tGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTc                                                                                    <  2:1252531/140‑1 (MQ=255)                                                   tGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTc                                                                                    <  2:805124/140‑1 (MQ=255)                                                     aaaCGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTa                                                                                         <  2:774159/133‑1 (MQ=255)                                                     aaaCGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTa                                                                                         >  1:774159/1‑133 (MQ=255)                                                      aaCGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTcaca                                                                                 <  1:1259340/140‑1 (MQ=255)                                                         gAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGt                                                                              <  1:1172396/140‑1 (MQ=255)                                                               ggCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGTCTGTGGGGTATTTCCAGGACCTCTCCTTTTCCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTccc                                                                        <  1:1408070/140‑1 (MQ=255)                                                               ggCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTccc                                                                        >  1:106039/1‑140 (MQ=255)                                                                 cATCTTGGTTCTGTACTGCCAGACACTTAAGCAACTGCCTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCAt                                                                      <  1:730890/140‑1 (MQ=255)                                                                 cATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCAt                                                                      <  2:199689/140‑1 (MQ=255)                                                                 cATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCAt                                                                      <  2:994064/140‑1 (MQ=255)                                                                 cATCTTGGTTCTGTAATGCCAGGCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCAt                                                                      <  2:524363/140‑1 (MQ=255)                                                                    cTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACt                                                                   >  1:983627/1‑140 (MQ=255)                                                                         ttCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCaa                                                              <  2:146970/140‑1 (MQ=255)                                                                              tAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGAt                                                           <  1:1236778/138‑1 (MQ=255)                                                                              tAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGAt                                                           >  2:1236778/1‑138 (MQ=255)                                                                                  gCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCAt                                                                      >  2:1506961/1‑123 (MQ=255)                                                                                  gCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCAt                                                                      <  1:1506961/123‑1 (MQ=255)                                                                                  gCCAGTCACTTAAGCAACTGACAGGCTCTTTTTAGGGGCGGTGGGGTACTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCa                                                     <  2:693412/140‑1 (MQ=255)                                                                                   ccggtcagtTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCAt                                                    >  2:1534867/4‑140 (MQ=255)                                                                                   ccAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCAt                                                    >  1:1492452/1‑140 (MQ=255)                                                                                   ccAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGATAAGTTCTCAAGTTGTGCCATACTTGCAAGATCGCGCAt                                                    >  2:521109/1‑140 (MQ=255)                                                                                      gtcagtTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAg                                                 <  1:955124/140‑1 (MQ=255)                                                                                      gtcagtTAAGCAACTGACTGGCGCTTTTTTGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAg                                                 <  1:548445/140‑1 (MQ=255)                                                                                       tcagtTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGc                                                >  1:1541599/1‑140 (MQ=255)                                                                                       tcagtTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGc                                                <  2:1492452/140‑1 (MQ=255)                                                                                        cagtTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCt                                               >  2:1403840/1‑140 (MQ=255)                                                                                         agtTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTc                                              <  1:1247052/140‑1 (MQ=255)                                                                                              aGCAACTGACTGGCTTTTTTTCGGGGCTGAGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGta                                         <  1:521109/140‑1 (MQ=255)                                                                                              aGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCAt                                                    <  1:1180355/129‑1 (MQ=255)                                                                                              aGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCAt                                                    >  2:1180355/1‑129 (MQ=255)                                                                                                cAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGtata                                       >  1:786080/1‑140 (MQ=255)                                                                                                 aaCTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATCTCACAAGTAGTCCCATACTTGCAAGCTCGCGCATCAGCTCCGGTATAc                                      >  2:548445/1‑140 (MQ=255)                                                                                                   ctgactgGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGTATACGt                                    >  1:1382935/1‑140 (MQ=255)                                                                                                     gactgGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGTATACGTcc                                  >  2:95153/1‑140 (MQ=255)                                                                                                     gactgGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGTATACGTcc                                  >  1:792717/1‑140 (MQ=255)                                                                                                       ctgGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGTATACGTCCCg                                <  2:786080/140‑1 (MQ=255)                                                                                                       ctgGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGTATACGTCCCg                                <  1:1380333/140‑1 (MQ=255)                                                                                                        tgGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGTATACGTCCCgg                               >  1:1223151/1‑140 (MQ=255)                                                                                                         ggCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGTATACGTCCCGGt                              >  2:1260455/1‑140 (MQ=255)                                                                                                            tctTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGTATACGTCCCGGTGaa                           <  2:792717/140‑1 (MQ=255)                                                                                                            tctTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGTATACGTCCCGGTGaa                           <  1:763633/140‑1 (MQ=255)                                                                                                            tctTTTTCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGTATACGTCCCGGTGaa                           <  1:1534867/140‑1 (MQ=255)                                                                                                              tttttCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGTATACGTCCCGGTGAAgc                         >  2:306180/1‑140 (MQ=255)                                                                                                                 ttCGGGGCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGTATACGTCCCGGTGAAGCGcc                      <  2:1223151/140‑1 (MQ=255)                                                                                                                     gggCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTAGCAAGATCGCGCATCAACTCCGGTAAACGTACCGGTGAAGCGCCCTGc                  >  2:463806/1‑140 (MQ=255)                                                                                                                      ggCTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGTATACGTCCCGGTGAAGCGCCCTGCa                 >  2:1079941/1‑140 (MQ=255)                                                                                                                        cTGTGGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGTATACGTCCCGGTGAAGCGCCCTGCAAt               <  1:1079941/140‑1 (MQ=255)                                                                                                                         tgtgGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGTATACGTCCCGGTGAAGCGCCCTGCAATg              >  1:1279744/1‑140 (MQ=255)                                                                                                                          gtgGGGTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGTATACGTCCCGGTGAAGCGCCCTGCAATGt             <  1:1206648/140‑1 (MQ=255)                                                                                                                             gggTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCACGATCGCGTCTCAGCTCCGGTATACGTCCCGGTGAAGCGCCCTGCAATGtcat          <  2:1541599/140‑1 (MQ=255)                                                                                                                             gggTATTTCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGTATACGTCCCGGTGAAGCGCCCTGCAATGtcat          <  1:1086525/140‑1 (MQ=255)                                                                                                                                  tttCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGTATACGTCCCGGTGAAGCGCCCTGCAATGTCATCAGCa     <  2:1087182/140‑1 (MQ=255)                                                                                                                                  tttCCAGGACCTCTCCTTTACCACTATCGAGAAGGCCGTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGTATACGTCCCGGTGAAGCGCCCTGCAATGTCATCAGCa     <  1:463806/140‑1 (MQ=255)                                                                                                                                   ttCCAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGTATACGTCCCGGTGAAGCGCCCTGCAATGTCATCAGCAt    >  2:224037/1‑140 (MQ=255)                                                                                                                                     ccAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGTATACGTCCCGGTGAAGCGCCCTGCAATGTCATCAGCATtc  >  1:196882/1‑140 (MQ=255)                                                                                                                                     ccAGGACCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGTATACGTCCCGGTGAAGCGCCCTGCAATGTCATCAGCATtc  >  1:1180007/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       AAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGTATACGTCCCGGTGAAGCGCCCTGCAATGTCATCAGCATTC  >  NC_000913/4502013‑4502284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑