Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	614,362	T→C	V69A (GTC→GCC)	entF →	apo‑serine activating enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	614,362	0	T	C	100.0%	244.2 / NA	70	V69A (GTC→GCC)	entF	apo‑serine activating enzyme
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (44/26);  total (44/26)

CCTCCGCCTGGAGCGTGGCGCATTACGTTGAGTTAACCGGAGAGGTTGATTCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGTCTGGCAGTGGGTCGATGATGCGCTGACGTTCGAACTGCCAGAAATTATCGACCTACGAACCAACATTGATCCGCACGGTACTGCGCAGGCATTAATGCAGGCGGATTTGCAACAAGATCTGCGCGTCGATA  >  NC_000913/614227‑614493                                                                                                                                        |                                                                                                                                    ccTCCGCCTGGAGCGTGGCGCATTACGTTGAGTTAACCGGAGAGGTTGATTCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTgg                                                                                                                                 >  1:265202/1‑140 (MQ=255)   tCCGCCTGGAGCGTGTCGCATTGCGATGATTTAACCGGAGAGGTTGATTCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCa                                                                                                                               <  1:647570/140‑1 (MQ=255)     cGCCTGGAGCGTGGCGCATTACGTTGAGTTAACCGGAGAGGTTGATTCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAGGATAACCGCGAAGCCTGGCAGt                                                                                                                             >  1:625745/1‑140 (MQ=255)     cGCCTGGAGCGTGGCGCATTACGTTGAGTTAACCGGAGAGGTTGATTCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTCTACGGATGATAACTGCGAAGCCTGGCAGt                                                                                                                             >  1:594062/1‑140 (MQ=255)         tGGAGCGTGGCGCATTAGGTTGTGTTAACCGAAGAGGTTTATTCGCCAATACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGt                                                                                                                         <  2:482150/140‑1 (MQ=255)               gTGGCGCATTACGTTGAGTTAACCGGAGAGGTTGATTCTCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGAAAACGGCGAAGCCTTGCAGTGGGTCgatga                                                                                                                   >  1:665906/1‑140 (MQ=255)               gTGGCGCATTACGTTGAGTTAACCGGAGAGGTTGATTCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCgatga                                                                                                                   >  2:1344633/1‑140 (MQ=255)               gTGGCGCATTACGTTGAGTTAACCGGAGAGGCTGATTCGCCATTACTGGCCCGCGCGGCGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCgatga                                                                                                                   >  2:143299/1‑140 (MQ=255)                tGGCGCATTACGTTGAGTTAACCGGAGAGGTTGATTCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAg                                                                                                                              >  2:1176718/1‑128 (MQ=255)                tGGCGCATTACGTTGAGTTAACCGGAGAGGTTGATTCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAg                                                                                                                              <  1:1176718/128‑1 (MQ=255)                tGGCGCATTACGTTGAGTTAACCGGAGAGGTTGATTCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCgatgat                                                                                                                  >  2:1346096/1‑140 (MQ=255)                tGGCGCATTACGTTGAGTTAACCGGAGAGGTTGATTCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTAAGGAAGATGACGGCGTAGCCTGTCAGTGGGTCgatgat                                                                                                                  >  1:482150/1‑140 (MQ=255)                 ggCGCATTACGTTGAGTTAACCGGAGAGGTTGATTCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGatgatg                                                                                                                 >  1:895140/1‑140 (MQ=255)                 ggCGCATTACGTTGAATTAACCCGACAGGTTGATTCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGatgatg                                                                                                                 <  1:477658/140‑1 (MQ=255)                   cgcATTACGTTGAGTTAACCGGAGAGGTTGATTCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGATGATgcg                                                                                                               <  1:1346096/140‑1 (MQ=255)                     cATTACGTTGAGTTAACCGGAGAGGTTGATTCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGATGATGCGCt                                                                                                             <  1:92937/140‑1 (MQ=255)                       ttACGTTGAGTTAACCGGAGAGGTTGATTCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTGa                                                                                                           <  1:350198/140‑1 (MQ=255)                          cGTTGAGTTAACCGGAGAGGTTGATTCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTGACGt                                                                                                        >  2:1172621/1‑140 (MQ=255)                          cGTTGAGTTAACAGGAGAGGTTGATTCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTGACGt                                                                                                        <  1:143299/140‑1 (MQ=255)                               aGTTAACCGGAGAGGTTGATTCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTGACGTTCGaa                                                                                                   >  2:1168965/1‑140 (MQ=255)                                  tAACCGGAGAGGTTGATTCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTGACGTTCGAACTg                                                                                                >  1:160356/1‑140 (MQ=255)                                         agagGTTGATTCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTGACGTTCGAACTGCCAGaaa                                                                                         >  2:38598/1‑140 (MQ=255)                                         agagGTTGATTCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTGACGTTCGAACTGCCAGaaa                                                                                         >  1:1435104/1‑140 (MQ=255)                                         agagGTTGATTCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTGACGTTCGAACTGCCAGaaa                                                                                         >  1:878880/1‑140 (MQ=255)                                               tGATTCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTGACGTTCGAACTGCCAGAAATTATCg                                                                                   <  2:1435104/140‑1 (MQ=255)                                                   tCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAGGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTAACGTTCGAACTGCAAGAAATTATCGACCt                                                                               >  1:518914/1‑140 (MQ=255)                                                   tCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTGACGTTCGAACTGCCAGAAATTATCGACCt                                                                               <  1:38598/140‑1 (MQ=255)                                                    cGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTGACGTTCGAACTGCCAGAAATTATCGACCTa                                                                              <  1:1344633/140‑1 (MQ=255)                                                         ttACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTGACGTTCGAACTGCCAGAAATTATCGACCTACGAAc                                                                         <  2:160356/140‑1 (MQ=255)                                                               gCCCGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTGACGTTCGAACTGCCAGAAATTATCGACCTACGAACCAACAt                                                                   >  1:132873/1‑140 (MQ=255)                                                                 ccGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTGACGTTCGAACTGCCAGAAATTATCg                                                                                   <  2:283777/122‑1 (MQ=255)                                                                 ccGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTGACGTTCGAACTGCCAGAAATTATCg                                                                                   >  1:283777/1‑122 (MQ=255)                                                                 ccGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTGACGTTCGAACTGCCAGAAATTATCGACCTACGAACCAACATTg                                                                 >  1:128381/1‑140 (MQ=255)                                                                    cgcgGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTGACGTTCGAGCTGCCAGGAATGATAGACCTAAGAACCAACATTGATc                                                              >  1:464280/1‑140 (MQ=255)                                                                    cgcgGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTGACGTTCGAACTGCCAGAAATTATCGACCTACGAACCAACATTGATc                                                              >  2:1512879/1‑140 (MQ=255)                                                                    cgcgGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTGACGTTCGAACTGCCAGAAATTATCGACCTACGAACCAACATTGATc                                                              >  1:833293/1‑140 (MQ=255)                                                                    cgcgGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTGACGTTCGAACTGCCAGAAATTATCGACCTACGAACCAACATTGATc                                                              >  2:190613/1‑140 (MQ=255)                                                                    cgcgGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTGACGTTCGAACTGCCAGAAATTATCGACCTACGAACCAACATTGATc                                                              >  1:255696/1‑140 (MQ=255)                                                                    cgcgGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTGACGTTCGAACTGCCAGAAATTATCGACCTAAGAACCAACATTGATc                                                              >  1:679922/1‑140 (MQ=255)                                                                        gtggtTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTGACGTTCGAACTGCCAGAAATTATCGACCTACGAACCAACATTGATCCGCa                                                          <  2:265202/140‑1 (MQ=255)                                                                         tggtTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTGACGTTCGAACTGCCAGAAATTATCGACCTACGAACCAACATTGATCCGCAc                                                         >  2:148246/1‑140 (MQ=255)                                                                                 ggACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTGACGTTCGAACTGCCAGAAAATATGGACATACGAACCAACATTGATCCGCACGGTACTgc                                                 >  2:472387/1‑140 (MQ=255)                                                                                  gACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTGACGTTCGAACTGCCAGAAATTATCGTCCTACGAACCAACATTGATCCGCACGGTACTgcg                                                <  2:132873/140‑1 (MQ=255)                                                                                   aCTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTGACGTTCGAACTGCCAGAAATTATCGACCTACGAACCAACATTGATCCGCa                                                          >  1:1143266/1‑129 (MQ=255)                                                                                   aCTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTGACGTTCGAACTGCCAGAAATTATCGACCTACGAACCAACATTGATCCGCa                                                          >  2:854993/1‑129 (MQ=255)                                                                                   aCTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTGACGTTCGAACTGCCAGAAATTATCGACCTACGAACCAACATTGATCCGCa                                                          <  1:854993/129‑1 (MQ=255)                                                                                   aCTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTGACGTTCGAACTGCCAGAAATTATCGACCTACGAACCAACATTGATCCGCa                                                          <  2:1143266/129‑1 (MQ=255)                                                                                   aCTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTGACGTTCGAACTGCCAGAAATTATCGACCTACGAACCAACATTGATCCGCACGGTa                                                     <  2:39270/134‑1 (MQ=255)                                                                                   aCTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTGACGTTCGAACTGCCAGAAATTATCGACCTACGAACCAACATTGATCCGCACGGTa                                                     >  1:39270/1‑134 (MQ=255)                                                                                   aCTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTGACGTTCGAACTGCCAGAAATTATCGACCTACGAACCAACATTGATCCGCACGGCACTgcgc                                               >  2:1257086/1‑140 (MQ=255)                                                                                       gcgcAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTGACGTTCGAACTGCCAGAAATTATCGACGTACGAACAAACGTTGATCCGCACGGTACTGCGCAGGc                                           >  1:504865/1‑140 (MQ=255)                                                                                       gcgcAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTGACGTTCGAACTGCCAGAAATTATCGACCTACGAACCAACATTGATCCGCACGGTACTGCGCAGGc                                           >  2:1115582/1‑140 (MQ=255)                                                                                        cgcAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTGACGTTCGAACTGCCAGAAATTATCGACCGACGAACCTACAGTGATCCGCATGGTACTGCGCAGGCa                                          >  1:456921/1‑140 (MQ=255)                                                                                             gCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTGACGTTCGAACTGCCAGAAATTATCGACCTACGAACCAACATTGATCCGCACGGTACTGCGCAGGCATTAAt                                     >  2:1456735/1‑140 (MQ=255)                                                                                                 aTACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTGACGTTCGAACTGCCAGAAATTATCGACCTACGAACCAACATTGATCCGCACGGTACTGCGCAGGCATTAATGCAg                                 >  2:1058478/1‑140 (MQ=255)                                                                                                 aTACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTGACGTTCGAACTGCCAGAAATTATCGACCTACGAACCAACATTGATCCGCACGGTACTGCGCAGGCATTAATGCAg                                 >  2:39792/1‑140 (MQ=255)                                                                                                  tACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTGACGTTCGAACTGCCAGAAATTATCGACCTACGAACCAACATTGATCCGCACGGTACTGCGCAGGCATTAATGCAgg                                <  2:128381/140‑1 (MQ=255)                                                                                                  tACGCTGCGGATGCGTTGTACGCAAGATAACCGCGGAGCTTGGCAGTGCGTCGATGATGCGCTGACGTTCGAACTGCCAGAAATTATCGACCTACGAACCAACATTGATCCGCACGGTACTGCGCAGGCATTAATGCAgg                                <  2:464280/140‑1 (MQ=255)                                                                                                   aCGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTGACGTTCGAACTGCCAGAAATTATCGACCTACGAACCAACATTGATCCGCACGGTACTGCGCAGGCATTAATGCAGGc                               >  1:276835/1‑140 (MQ=255)                                                                                                       tGCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTGACGTTCGAACTGCCAGAAATTATCGACCTACGAACCAACATTGATCCGCACGGTACTGCGCAGGCATTAATGCAGGCGGAt                           >  1:355760/1‑140 (MQ=255)                                                                                                        gCGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTGACGTTCGAACTGCCAGAAATTATCGACCTACGAACCAACATTGATCCGCACGGTACTGCGCAGGCATTAATGCAGGCGGAtt                          <  1:1172621/140‑1 (MQ=255)                                                                                                         cGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTGACGTTCGAACTGCCAGAAATTATCGACCTACGAACCAACATTGATCCGCACGGTACTGCGCAGGCATTAATGCAGGCGGAttt                         <  1:1528372/140‑1 (MQ=255)                                                                                                         cGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTGACGTTCGAACTGCCAGAAATTATCGACCTACGAACCAACATTGATCCGCACGGTACTGCGCAGGCATTAATGCAGGCGGAttt                         <  1:39792/140‑1 (MQ=255)                                                                                                         cGGATGCGTTTTACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTGACGTTCGAACTGCCAGAAATTATCGACCTACGAACCAACATTGATCCGCACGGTACTGCGCAGGCATTAACGCAGGCGGAttt                         >  1:108581/1‑140 (MQ=255)                                                                                                         cGGATGCGTTTTACCGAAGATAACGGCGAATCTTGGCAGTGGGTCGATGCTGCGCTTACGTTACAACTGCCAGAAATTATCGACCTACGAACCAACATTGACCCGCACGCTACT‑‑GCAGGCATTAATGCAGGCGGATTTGc                       <  2:953117/140‑1 (MQ=255)                                                                                                             tGCGTTTTACGGAAGATGACGGCGAAGCCTGACAGGGGGTCGATGATGTGCTGACGTTCGAACTGCCAGAAATTATCGACCTACGAACCAACATTGATCCGCACGGTACTGCGCAGGCATTAATGCAGGCGGATTTGcaa                     <  2:456921/140‑1 (MQ=255)                                                                                                                   ttACGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTGACGTTCGAACTGCCAGAAATTATCGACCTACGAACCAACATTGATCCGCACGGTACTGCGCAGGCATTAATGCAGGCGGATTTGCAACAAGAt               >  1:7735/1‑140 (MQ=255)                                                                                                                      cGGAAGATAACGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTGACGTTCGAACTGCCAGAAATTATCGACCTACGAACCAACATTGATCCGCACGGTACGGCGGAGGAATTAATGCAGGCGGATTTGCAACAAGATCTg            >  2:472608/1‑140 (MQ=255)                                                                                                                              aaCGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTGACGTTCGAACTGCCAGAAATTATCGACCTACGAACCAACATTGATCCGCACGGTACTGCGCAGGCATTAATGCAGGCGGATTTGCAACAAGATCTGCGCGTCGa    >  2:1087668/1‑140 (MQ=255)                                                                                                                               aCGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTGACGTTCGAACTGCCAGAAATTATCGACCTACGAACCAACATTGATCCGCACGGTACTGCGCAGGCATTAATGCAGGCGGATTTGCAACAAGATCTGCGCGTCGAt   <  2:878880/140‑1 (MQ=255)                                                                                                                                cGGCGAAGCCTGGCAGTGGGTCGATGATGCGCTGACGTTCGAACTGCCAGAAATTATCGACCTACGAACCAACATTGATCCGCACGGTACTGCGCAGGCATTAATGCAGGCGGATTTGCAACAAGATCTGCGCGTCGATa  >  1:1320390/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                    CCTCCGCCTGGAGCGTGGCGCATTACGTTGAGTTAACCGGAGAGGTTGATTCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGAAGTCTGGCAGTGGGTCGATGATGCGCTGACGTTCGAACTGCCAGAAATTATCGACCTACGAACCAACATTGATCCGCACGGTACTGCGCAGGCATTAATGCAGGCGGATTTGCAACAAGATCTGCGCGTCGATA  >  NC_000913/614227‑614493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑