BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	706,889	A→G	Y266C (TAC→TGC)	glnS →	glutamine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	706,889	0	A	G	97.0%	340.5 / NA	99	Y266C (TAC→TGC)	glnS	glutamine‑‑tRNA ligase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (54/42); minor base C (0/3); total (54/45)
Fisher's exact test for biased strand distribution p-value = 9.05e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

TGCCCTGGAAGGTATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGACCATTTCCGGTCTGCGTCGTCGTGGTTACACTGCGGCTTCTATTCGT  >  NC_000913/706752‑707016

tGCCCTGGAAGGTATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATgca                                                                                                                               <  2:1386420/140‑1 (MQ=255)

 gCCCTGGAAGGTATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATgcac                                                                                                                              <  1:57414/140‑1 (MQ=255)

  cccTGGAAGGTATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCAcc                                                                                                                             >  2:1089273/1‑140 (MQ=255)

     tGGAAGGTATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTg                                                                                                                          >  2:236674/1‑140 (MQ=255)

     tGGAAGGTATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTg                                                                                                                          <  1:1216349/140‑1 (MQ=255)

            tATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCa                                                                                                                   >  2:203395/1‑140 (MQ=255)

            tATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCa                                                                                                                   >  1:1385898/1‑140 (MQ=255)

            tATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCa                                                                                                                   >  2:1301110/1‑140 (MQ=255)

                gacagctctctGTGTACGCTTGAGTTCCAGGCCAAACGTTGTCTGTACCACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCg                                                                                                               <  2:521839/135‑1 (MQ=255)

                aCGCACTCTCTGTGTACCCTTGTGTTCCAGGCCAATCGTAGTCTGTACTACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCg                                                                                                               <  2:417163/140‑1 (MQ=255)

                 cGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTATCGCGCCTGAATCTGGGATGCTCCGTGATGTCCAAGCGt                                                                                                              >  2:432390/1‑140 (MQ=255)

                    aCTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAg                                                                                                           <  1:1469074/140‑1 (MQ=255)

                     ctctctGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGt                                                                                                          >  1:1503598/1‑140 (MQ=255)

                        tctGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGa                                                                                                       >  2:1161423/1‑140 (MQ=255)

                              tACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTAGAATGCACCGTAATGTCCAAGCGTAAGTTGAACctgc                                                                                                 >  1:703347/1‑140 (MQ=255)

                                cGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGTATGCACCCTGAGGTTCAAGCGTAAGTTGAAccctgtc                                                                                               >  1:521839/1‑135 (MQ=255)

                                cGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGCATGCACCGTGAAGTGCAAGCGCAAGTTGAACctgctg                                                                                               >  1:534559/1‑140 (MQ=255)

                                cGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACctgctg                                                                                               >  2:152957/1‑140 (MQ=255)

                                cGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACctgctg                                                                                               >  1:874372/1‑140 (MQ=255)

                                cGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCATGAGGTTCAAGCGGAAGTTGAACctgctg                                                                                               >  1:611807/1‑140 (MQ=255)

                                  cTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGt                                                                                             >  1:196729/1‑140 (MQ=255)

                                    tGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAAGGCACCGTGATGTCCAAGCGTATGTTGAACCTGCTGGTGa                                                                                           >  2:658956/1‑140 (MQ=255)

                                      aGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCAGCGTGATGTCCATGCATAAGTTGAACCTGCTGGTGAcc                                                                                         >  1:397927/1‑140 (MQ=255)

                                      aGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGAcc                                                                                         <  1:236674/140‑1 (MQ=255)

                                                  aCCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCTTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGtt                                                                             <  2:1493998/140‑1 (MQ=255)

                                                  aCCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGtt                                                                             >  2:236908/1‑140 (MQ=255)

                                                   ccGTCGTCAGTACGACAGGGTACTGGACAACCTGACGATACCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTg                                                                            <  2:750188/140‑1 (MQ=255)

                                                      tcgtcTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAg                                                                         >  1:1385403/1‑140 (MQ=255)

                                                       cgtcTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAgg                                                                        >  2:803398/1‑140 (MQ=255)

                                                         tcTGTACGACTGGGTCCTGGACAACCTCAAGATTCCTTTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCt                                                                      <  1:432390/140‑1 (MQ=255)

                                                             tACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCACGCGTAAGTGGAACCTGTTGGTGACCCACAAACACGTTGAAGGCTGGGa                                                                  >  2:595924/1‑140 (MQ=255)

                                                             tACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGa                                                                  <  2:821859/140‑1 (MQ=255)

                                                              aCGACTGGGTACTGGACTACATTACGATTCCGGTTGACCTGCGCCAGTGTGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGAt                                                                 <  2:464096/140‑1 (MQ=255)

                                                               cGACTGGGTATTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCt                                                                      >  1:52277/1‑134 (MQ=255)

                                                               cGACTGGGTATTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCt                                                                      <  2:52277/134‑1 (MQ=255)

                                                               cGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCt                                                                      >  1:782300/1‑134 (MQ=255)

                                                               cGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCt                                                                      <  2:782300/134‑1 (MQ=255)

                                                                   tGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGtt                                                                             >  1:1011820/1‑123 (MQ=255)

                                                                     ggTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCcgc                                                          <  2:874372/140‑1 (MQ=255)

                                                                     ggTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCcgc                                                          >  1:37440/1‑140 (MQ=255)

                                                                      gTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCcgcg                                                         <  1:803398/140‑1 (MQ=255)

                                                                       tACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGt                                                        >  1:152042/1‑140 (MQ=255)

                                                                          tGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATg                                                     >  2:160115/1‑140 (MQ=255)

                                                                          tGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATg                                                     <  2:37440/140‑1 (MQ=255)

                                                                            gACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGcc                                                   >  1:110037/1‑140 (MQ=255)

                                                                             acaacaTCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCg                                                  <  2:110037/140‑1 (MQ=255)

                                                                               aacaTCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCGTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGAc                                                <  2:1045884/140‑1 (MQ=255)

                                                                               aacaTCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGAc                                                <  1:160115/140‑1 (MQ=255)

                                                                               aacaTCACGATTCCTCTTCACCTGCTCCAGTATGAGTGCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGAc                                                <  1:645488/140‑1 (MQ=255)

                                                                                   tCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCGCGTTGAAGGCTTGGATGACCCGCGTATGCCGACCAtt                                            >  1:599959/1‑140 (MQ=255)

                                                                                    cACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGACCAttt                                           <  2:1045060/140‑1 (MQ=255)

                                                                                    cACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGACCAttt                                           >  2:1402918/1‑140 (MQ=255)

                                                                                    cACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGACCAttt                                           >  2:385386/1‑140 (MQ=255)

                                                                                    cACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGAGAGGACCCGTGTATGCCGACCAttt                                           >  1:625873/1‑140 (MQ=255)

                                                                                      cGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGACCATTTcc                                         >  2:1530316/1‑140 (MQ=255)

                                                                                            cTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGACCATTTCCGGTCTg                                   >  1:1430113/1‑140 (MQ=255)

                                                                                              gTTCACCCGCGCCAGTATGAGTTCTCGCGCCCGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGACCATTTCCGGTCTGcg                                 <  1:152957/140‑1 (MQ=255)

                                                                                              gTTCACCCGCGCCAGTATGAGTGCTCGCGCCCGGATCTGAAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGACCATTTCCGGTCTGcg                                 <  2:703347/140‑1 (MQ=255)

                                                                                                  ccccGCGCCAGTATGAGTTCTCGCGCCTGAATCCGAAATCCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGACCATTTCCGGTCTGcgtcgt                             <  2:669184/139‑1 (MQ=255)

                                                                                                  ccccGCGCCAGTATGAGTTCTCCCGCCTGAATCTGTAATCCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGACCATTTCCGGTCTGcgtcgt                             <  2:534559/139‑1 (MQ=255)

                                                                                                  aCCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGACCATTTCCGGTCTGcgtcgt                             <  1:1161423/140‑1 (MQ=255)

                                                                                                  aCCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGACCATTTCCGGTCTGcgtcgt                             <  1:928675/140‑1 (MQ=255)

                                                                                                  aCCCGCGCCAGTATGAGTTATCGCGCCTGAATCTGGAATGCTCCGTGATGTCCAAGCGTAAGTTGAACCTGATGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGACCATTTCCGGTCTGcgtcgt                             <  2:1532207/140‑1 (MQ=255)

                                                                                                  aCCCGCGCCAGTATGAGCTCTCCCGCCTGAATCTGGAATCCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGACCATTTCCGGTCTGcgtcgt                             <  2:625873/140‑1 (MQ=255)

                                                                                                   cccGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGACCATTTCCGGTCTGcgtcgtc                            >  2:288116/1‑140 (MQ=255)

                                                                                                   cccGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGACGACCCGCGTATGCCGACCATTGCCGGTCTGcgtcgtc                            >  1:669184/1‑140 (MQ=255)

                                                                                                    ccGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGACCATTTCCGGTCTGcgtcgtcg                           >  1:1399857/1‑140 (MQ=255)

                                                                                                    ccGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGACCATTTCCGGTCTGcgtcgtcg                           >  1:905796/1‑140 (MQ=255)

                                                                                                    ccGCGCCAGTATGAGATCTCGCCCCTGAAACTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGACCATTTCCGGTCTGcgtcgtcg                           <  1:658956/140‑1 (MQ=255)

                                                                                                      gcgcCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGACCATTTCCGGTCTGcgtcgt                             >  1:1030549/1‑136 (MQ=255)

                                                                                                      gcgcCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGACCATTTCCGGTCTGcgtcgt                             <  2:1030549/136‑1 (MQ=255)

                                                                                                      gcgcCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGACCATTTCCGGTCTGCGTCGTCGTg                         >  2:1318410/1‑140 (MQ=255)

                                                                                                       cgcCAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGACCATTTCCGGTCTGCGTCGTCGTgg                        >  2:1135564/1‑140 (MQ=255)

                                                                                                         ccAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGTCCGACAAGCACGTTGAAGGCGGGGATGACGCGCGTCTGCCGACCATTTCCGGTCTGCGTCGTCGTGGtt                      >  2:706841/1‑140 (MQ=255)

                                                                                                         ccAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGACCATTTCCGGTCTGCGTCGTCGTGGtt                      >  2:383860/1‑140 (MQ=255)

                                                                                                         ccAGTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGACCATTTCCGGTCTGCGTCGTCGTGGtt                      >  1:1221426/1‑140 (MQ=255)

                                                                                                            gTATGAGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGACCATTTCCGGTCTGCGTCGTCGTGGTTaca                   >  1:993048/1‑140 (MQ=255)

                                                                                                                 aGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGACCATTTCCGGTCTGCGTCGTCGTGGTTACACTGCg              <  1:260527/140‑1 (MQ=255)

                                                                                                                 aGTTCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGCATGCCGACCATTTCCGGTCTGCGTCGTCGTGGTTACACTGCg              <  2:1358500/140‑1 (MQ=255)

                                                                                                                   ttCTCGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGACCACTTCCGGTCTGCGTCGTCGTGGTTACACTGCGGc            <  1:786290/140‑1 (MQ=255)

                                                                                                                    tctcGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGACCATTTCCGGTCTGCGTCGTCGTGGTTACACTGCGGc            >  2:34155/1‑139 (MQ=255)

                                                                                                                    tctcGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGACCATTTCCGGTCTGCGTCGTCGTGGTTACACTGCGGc            <  1:34155/139‑1 (MQ=255)

                                                                                                                     ctcGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCCACCATTTCCGGTCTGCGTCGTCGTGGTTACACTGCGGCtt          <  2:993048/140‑1 (MQ=255)

                                                                                                                     ctcGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATAACCCGCGTATGCCGACCATTTCCGGTCTGCGTCGTCGTGGTTACACTGCGGCtt          <  2:852695/140‑1 (MQ=255)

                                                                                                                     ctcGCGCATGAATCTGGAATGCTCCGTGATGTCCAAGCGCAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGACCATTTCCGGTCTGCGTCGTCGTGGTTACACTGCGGCtt          <  2:529775/140‑1 (MQ=255)

                                                                                                                      tcGCGCCTGAATCTGGAATGCATCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGTCCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGACCATTTCCGGTCTGCGTCGTCGTGGTTACACTGCGGCTTc         <  1:706841/140‑1 (MQ=255)

                                                                                                                      tcGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGACCATTTCCGGTCTGCGTCGTCGTGGTTACACTGCGGCTTc         <  1:383860/140‑1 (MQ=255)

                                                                                                                      tcGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGACCATTTCCGGACTGTGTCGTCGTGGTTACACTGCGGCTTc         >  1:705298/1‑140 (MQ=255)

                                                                                                                      tcGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGAACATGTCCGGTCTGCGTGGTCGGGGTTACACTGCGGCTTc         >  2:507200/1‑140 (MQ=255)

                                                                                                                      tcGCGCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCATATGCCGACCATGTCCTGTCTGCGTTGTCGAGGTTACACTGCGGCTTc         >  2:541181/1‑140 (MQ=255)

                                                                                                                       cgcgCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGACCATTTCCGGTCTGCGTCGTCGTGGTTACACTGCGGCTTCt        >  1:71987/1‑140 (MQ=255)

                                                                                                                       cgcgCCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTCACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGACCATTTCCGGTCTGCGTCGTCGTGGTTACACTGCGGCTTCt        >  1:1429669/1‑140 (MQ=255)

                                                                                                                        gcgcCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGACCATTTCCGGTCTGCGTCGTCGTGGTTACACTGCGGCTTCTa       <  1:1045931/140‑1 (MQ=255)

                                                                                                                        gcgcCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGACCATTTCCGGTCTGCGTCGTCGTGGTTACACTGCGGCTTCTa       <  2:1430113/140‑1 (MQ=255)

                                                                                                                        gcgcCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGACCATTTCCGGTCTGCGTCGTCGTGGTTACACTGCGGCTTCTa       <  1:203395/140‑1 (MQ=255)

                                                                                                                        gcgcCTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGACCATTTCCGGTCTGCGTCGTCGTGGTTACACTGCGGCTTCTa       <  1:288116/140‑1 (MQ=255)

                                                                                                                           ccTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGACCATTTCCGGTCTGCGTCGTCGTGGTTACACTGCGGCTTCTATTc    >  1:1441411/1‑140 (MQ=255)

                                                                                                                            cTGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATG‑CCCGCGTATGCCGACCATTTCCGGTCTGCGTCGTCGTGGTTACACTGCGGCTTCTATTCGt  <  2:196729/140‑1 (MQ=255)

                                                                                                                             tGAATCTGGAATGCACCGTGATGTCTAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGACCATTTCCGGTCTGCGTCGTCGTGGTTACACTGCGGCTTCTATTCGt  <  1:538877/140‑1 (MQ=255)

                                                                                                                             tGAATCTGGAATGCACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGACCATTTCAGGTTTGCGTCGTCGTGTTTACACTGCGGCTTCTATTCGt  >  2:538877/1‑140 (MQ=255)

TGCCCTGGAAGGTATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGACCATTTCCGGTCTGCGTCGTCGTGGTTACACTGCGGCTTCTATTCGT  >  NC_000913/706752‑707016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`