Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	731,871	T→A	N763E (AAT→GAA)	rhsC →	rhs element protein RhsC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	731,871	0	T	A	100.0%	112.5 / NA	51	N763E (AAT→GAA)	rhsC	rhs element protein RhsC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (29/22);  total (29/22)

GTATGACGAACGCGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCATCCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGA  >  NC_000913/731739‑731960                                                                                                                                     |                                                                                          gTATGACGAACGCGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCACCCGCAGACGGAAGCACTGc                                                                                    >  1:1108442/1‑140 (MQ=255)  tATGACGAACGCGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGACACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCACCCGCAGACGGAAGCACTGct                                                                                   >  1:1138395/1‑140 (MQ=21)      aCGAACGCGGCTGGCTGACAGAGATAAGCTATATCAGTGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTgg                                                                               <  1:689131/140‑1 (MQ=18)      aCGAACGCGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTgg                                                                               <  2:1167571/140‑1 (MQ=18)       cGAACGCGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGgc                                                                              <  1:300039/140‑1 (MQ=18)         aaCGCGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGgcag                                                                            >  2:891553/1‑140 (MQ=255)          aCGCGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGgcagc                                                                           >  1:1243675/1‑140 (MQ=255)            gcgGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCAt                                                                         <  2:246534/140‑1 (MQ=18)            gcgGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCAt                                                                         <  2:1441305/140‑1 (MQ=18)            gcgGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCAt                                                                         <  2:201274/140‑1 (MQ=18)            gcgGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCAt                                                                         >  1:9576/1‑140 (MQ=255)                   gctgACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCa                                                                  <  1:261174/140‑1 (MQ=18)                     tgACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGa                                                                <  1:1191664/140‑1 (MQ=18)                     tgACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGa                                                                <  2:9576/140‑1 (MQ=18)                     tgACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGa                                                                <  1:108886/140‑1 (MQ=18)                     tgACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGa                                                                <  1:891553/140‑1 (MQ=18)                             aTCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTa                                                        >  1:222749/1‑140 (MQ=255)                               cAGCCATATCAGCGAAGGACACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACa                                                      <  2:1138395/140‑1 (MQ=18)                                 gCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAAc                                                    >  2:1474301/1‑140 (MQ=255)                                     tatCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGcgc                                                >  1:1384212/1‑138 (MQ=18)                                            gAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGTAGCATGAAACCGGAAATGCGTACAACGCGCAGGGGCt                                         >  1:478355/1‑140 (MQ=18)                                              aGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTgg                                       >  1:1038874/1‑140 (MQ=18)                                                ggCACCGGGTGACGGCGCACTAAGGCTATCACAGTAATGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCg                                     <  1:435077/140‑1 (MQ=17)                                                  cACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGaa                                   <  1:331357/140‑1 (MQ=18)                                                  cACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGaa                                   <  2:273064/140‑1 (MQ=17)                                                     cGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCg                                <  2:1243675/140‑1 (MQ=18)                                                       ggTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCt                              <  2:1384212/140‑1 (MQ=17)                                                       ggTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCt                              <  2:174736/140‑1 (MQ=18)                                                                  aCTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCACCCGCAGACGGAAGTACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGAc                   <  2:1108442/140‑1 (MQ=17)                                                                   cTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACa                  >  1:1078980/1‑140 (MQ=17)                                                                      tGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGTGAACCGCTGTATACCGGACAGcc               >  1:1437614/1‑140 (MQ=14)                                                                        gTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTg             >  1:1013213/1‑140 (MQ=14)                                                                         ttATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGc            >  2:176902/1‑140 (MQ=14)                                                                             gACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGAGGCTGGCGAACGGCAGTATACAGGACAGCCTGCccgc        >  1:448301/1‑140 (MQ=14)                                                                              aCAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCccgcc       >  1:1408995/1‑140 (MQ=14)                                                                                aGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGt     >  2:980841/1‑140 (MQ=11)                                                                                aGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGt     >  1:301775/1‑140 (MQ=11)                                                                                  tAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATCCCGGACAGCCTGCCCGCCGTgg   >  1:699402/1‑140 (MQ=11)                                                                                  tAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCccgc        <  2:1487523/135‑1 (MQ=11)                                                                                  tAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCccgc        >  1:1487523/1‑135 (MQ=11)                                                                                  tAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTgg   >  1:36136/1‑140 (MQ=11)                                                                                  tAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTgg   <  1:980841/140‑1 (MQ=11)                                                                                  tAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTgg   >  1:1531134/1‑140 (MQ=11)                                                                                  tAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTgg   >  1:1374378/1‑140 (MQ=11)                                                                                  tAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTgg   >  1:1157150/1‑140 (MQ=11)                                                                                  tAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTgg   >  1:1105701/1‑140 (MQ=11)                                                                                  tAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTgg   >  1:826182/1‑140 (MQ=11)                                                                                  tAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCCCTGCATGCCGGACTGCCTGCCCGCCGTgg   >  1:710746/1‑140 (MQ=11)                                                                                   aaaGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGcc           >  2:1159202/1‑131 (MQ=11)                                                                                   aaaGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGcc           <  1:1159202/131‑1 (MQ=11)                                                                                   aaaGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGa  >  1:354930/1‑140 (MQ=11)                                                                                                                                     |                                                                                          GTATGACGAACGCGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGACGGTGCACTATGGTTATGACAGTAAAGGCCGCCTCGCCAGTGAACACCTGACGGTGCATCATCCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGA  >  NC_000913/731739‑731960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑