Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	830,264	A→G	L127P (CTC→CCC)	cecR ←	DNA‑binding transcriptional dual regulator CecR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	830,264	0	A	G	95.7%	226.4 / ‑4.2	70	L127P (CTC→CCC)	cecR	DNA‑binding transcriptional dual regulator CecR
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base G (33/33);  total (36/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGTTCCTGCAGGCGCGAAGGATCAGTTCACGGATGGCAGCCCGATCAGGCTGTGGTTGCGCGAACA  >  NC_000913/830131‑830393                                                                                                                                      |                                                                                                                                  cGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCATGTGGTAGGCTGCCGTGGGAGAGGgctgct                                                                                                                             >  1:439785/1‑140 (MQ=255) cGCATGGGTATGAAGGACCATGCGGGTGTCACTGGCGTCCCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGGgctgct                                                                                                                             <  2:439785/140‑1 (MQ=255)     tGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGTGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGGGCTGCTCACg                                                                                                                         <  2:264035/140‑1 (MQ=255)     tGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGGGCTGCTCACg                                                                                                                         <  1:1460582/140‑1 (MQ=255)     tGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGGGCTGCTCACg                                                                                                                         <  2:52608/140‑1 (MQ=255)           tGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTATCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGGGCTGCTCACGGga                                                                                                                      >  2:1225171/1‑137 (MQ=255)           tGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTATCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGGGCTGCTCACGGga                                                                                                                      <  1:1225171/137‑1 (MQ=255)           tGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTTGTGCACCACGTGATAGGCTGCCGTGGGAGAGGGCTGCTCACGGGAGAt                                                                                                                   >  2:470232/1‑140 (MQ=255)             aaGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGGGCTGCTCACGGGAGATaa                                                                                                                 >  1:1031425/1‑140 (MQ=255)                 aTCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCATCAGGTGGTTGGCCGCCCTGGGAGAGAGCTGATCACGGGAGATAAACtt                                                                                                             >  2:448818/1‑140 (MQ=255)                 aTCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACtt                                                                                                             >  2:1402108/1‑140 (MQ=255)                 aTCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCAACAGGTGGTGGGCTGCCATGGGAGAGCGCTGTTCACGGGAGATAAACtt                                                                                                             >  2:626190/1‑140 (MQ=255)                  tCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACGAGGTGGTAAGCTGCCGAGGGAGAGGTCTGCACACGGGAGATAAACTTg                                                                                                            >  2:440387/1‑140 (MQ=255)                  tCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGGGCTGCTCAc                                                                                                                          >  1:1374622/1‑126 (MQ=255)                  tCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGGGCTGCTCAc                                                                                                                          <  2:1374622/126‑1 (MQ=255)                  tCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACTTg                                                                                                            >  1:925827/1‑140 (MQ=255)                  tCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACTTg                                                                                                            >  1:1048132/1‑140 (MQ=255)                   cATGCGGGCGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACTTGc                                                                                                           >  2:54359/1‑140 (MQ=255)                    aTGCGGGTGTCATTGTCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACTTGCt                                                                                                          <  1:448818/140‑1 (MQ=255)                    aTGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACTTGCt                                                                                                          <  2:1048132/140‑1 (MQ=255)                     tGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGGCGTGGGATAGGGCTACTCACGAGAGATAAACTTGCTg                                                                                                         >  1:736000/1‑140 (MQ=255)                     tGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGACTGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACTTGCTg                                                                                                         >  1:971550/1‑140 (MQ=255)                      gCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACTTGCTGa                                                                                                        >  2:82944/1‑140 (MQ=255)                        gggTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACTTGCTGAgg                                                                                                      >  2:1411686/1‑140 (MQ=255)                          gtgtCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACTTGCTGAGGtt                                                                                                    >  2:1180496/1‑140 (MQ=255)                            gtCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACTTGCTGAGGTTGa                                                                                                  <  1:1402108/140‑1 (MQ=255)                            gtCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACTTGCTGAGGTTGa                                                                                                  <  1:940498/140‑1 (MQ=255)                            gtCATTGGCGTCGCAGACGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACTTGCTGAGGTTGa                                                                                                  <  2:926803/140‑1 (MQ=255)                              cATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACg                                                                                                <  1:168782/140‑1 (MQ=255)                                ttGGCGTCGCAGCCGTTCCAGGCGGGAATCAGGCGTGACAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTAACGGGAGATAAACTTGCTGAGGTTGACGgt                                                                                              <  1:692600/140‑1 (MQ=255)                                ttGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGgt                                                                                              <  1:82944/140‑1 (MQ=255)                                ttGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCGCGGGAGTTAAACTGGCTGAGGTTGACGgt                                                                                              >  1:411213/1‑140 (MQ=255)                                ttGGCGTCGCAGCCGGTCCAGGAGGCAATCACGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGgt                                                                                              <  2:669799/140‑1 (MQ=255)                                       cGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACTTGCTGAGGTTGa                                                                                                  >  1:1045523/1‑129 (MQ=255)                                       cGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACTTGCTGAGGTTGa                                                                                                  <  2:1045523/129‑1 (MQ=255)                                         cAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTg                                                                                     >  1:919911/1‑140 (MQ=255)                                               gTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGag                                                                               <  1:1180496/140‑1 (MQ=255)                                               gTCCAGGCGGCAATCAGGCGTGTAAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGag                                                                               <  1:1534954/140‑1 (MQ=255)                                                          aaTCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCa                                                                    >  1:916237/1‑140 (MQ=255)                                                          aaTCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGACTGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCa                                                                    <  2:971550/140‑1 (MQ=255)                                                                    gtCAGGTGGCTGTGTCGCGGACTAAGCACGTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGTTCCTGCa                                                          <  1:626190/140‑1 (MQ=255)                                                                    gtCAGGTGCCTGTGTAGGGGACTAATCACCGTTCCCTGCCCCAGGTGGAAGGCTGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGTTCCTGCa                                                          <  2:736000/140‑1 (MQ=255)                                                                      cAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGTTCCTGCAgg                                                        <  1:1351679/140‑1 (MQ=255)                                                                        ggTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGTTCCTGCAGgcg                                                      <  2:919911/140‑1 (MQ=255)                                                                         gTGGCTGTGTAGCGGACTAATCCCCCGTTCGTGCACCCGGTGGTAGGCTGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGTTCCTGCAGgcgc                                                     <  1:470232/140‑1 (MQ=255)                                                                              tgtgTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGTTCCTGCAGGCGCGAAgg                                                >  1:1448786/1‑140 (MQ=255)                                                                               gtgtAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGTTCCTGCAGGcgcg                                                    >  2:1378754/1‑135 (MQ=255)                                                                               gtgtAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGTTCCTGCAGGcgcg                                                    <  1:1378754/135‑1 (MQ=255)                                                                                tgtAGCGGACTAATCCCCTGTTCGTCCACCAGGTGGTTGGCTGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGTTCCTGCAGGCGCGAAGGAt                                              <  1:440387/140‑1 (MQ=255)                                                                                   aGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGTTCCTGCAGGCGCGAAGGATCAg                                           >  2:386799/1‑140 (MQ=255)                                                                                                ccTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGTTCCTGCAGGCGCGAAGGATCAGTTCACGGATGGCa                              <  1:54359/140‑1 (MQ=255)                                                                                                 cTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGTTCCTGCAGGCGCGAAGGATCAGTTCACGGATGGCAg                             >  1:858568/1‑140 (MQ=255)                                                                                                 cTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGTTCCTGCAGGCGCGAAGGATCAGTTCACGGATGGCAg                             >  2:1391509/1‑140 (MQ=255)                                                                                                 cTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGTTCCTGCAGGCGCGAAGGATCAGTTCACGGATGGCAg                             >  1:41406/1‑140 (MQ=255)                                                                                                    ttCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCGTAATCATGCTCCCGCAGGCGCGAAGCATCACTTCACGGATGGCAGccc                          >  2:638339/1‑140 (MQ=255)                                                                                                      cGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGTTCCTGCAGGCGCGAAGGATCAGTTCACGGATGGCAGCCCGa                        >  2:1339929/1‑140 (MQ=255)                                                                                                      cGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGTTCCAGCAGGCGGGAAAGAGCAGTTCACGGATGGCAGCCCGa                        >  1:393531/1‑140 (MQ=255)                                                                                                       gTGCACCAGGTGGTAGGCTGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGTTCCTGCAGGCGCGAAGGATCAGTTCACGGATGGCAGCCCGAt                       <  1:1339929/140‑1 (MQ=255)                                                                                                       gTGCACCAGGTGGTAGGCTGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGTTCCTGCAGGCGCGAAGGATCAGTTCACGGATGGCAGCCCGAt                       <  2:925827/140‑1 (MQ=255)                                                                                                       gTGCACCAGGTGGTAGGCTGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACAGTGTCATCCTGGGTGAGCAGCTTAATCATGTTCCTGCAGGCGCGAAGGATCAGTTCACGGATGGCAGCCCGAt                       <  2:1031425/140‑1 (MQ=255)                                                                                                           aCCAGGTGGTAGGCTGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGTTCCTGCAGGCGCGAAGGATCAGTTCACGGATGGCAGCCCGATCAgg                   >  2:1268892/1‑140 (MQ=255)                                                                                                               ggtggtAGGCTGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGTTCCTGCAGGCGCGAAGGATCAGTTCACGGATGGCAg                             >  2:1296227/1‑126 (MQ=255)                                                                                                               ggtggtAGGCTGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGTTCCTGCAGGCGCGAAGGATCAGTTCACGGATGGCAg                             <  1:1296227/126‑1 (MQ=255)                                                                                                               ggtggtAGGCTGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGTTCCTGCAGGCGCGAAGGATCAGTTCACGGATGGCAGCCCGATCAGGCtgt               >  2:1496508/1‑140 (MQ=255)                                                                                                                 tggtAGGCTGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGTTCCTGCAGGCGCGAAGGATCAGTTCACGGATGGCAGCCCGATCAGGCTGTgg             <  1:1411686/140‑1 (MQ=255)                                                                                                                     aGGCTGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGTTCCTGCAGGCGCGAAGGATCAGTTCACGGATGGCAGCCCGATCAGGCTGTGGTTgc         <  2:1448786/140‑1 (MQ=255)                                                                                                                       gCTGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGTTCCTGCAGGCGCGAAGGATCAGTTCACGGATGGCAGCCCGATCAGGCTGTGGTTgcgc       >  1:1065880/1‑140 (MQ=255)                                                                                                                         tGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGTTCCTGCAGGCGCGAAGGATCAGATCACGGATGGCAGCCCGATCAGGCTGTGGTTGCGCGa     >  2:1282425/1‑140 (MQ=255)                                                                                                                         tGCCGTGGGAGAGGGCTGCTCACGGGAGATAAACTTGCTGAGGCTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGTTCCTGCAGGCGCGAAGGATCAGTTCACGGAAGGCACCCCGATCAGGCTGTGCATgcgcca     >  2:114608/1‑138 (MQ=255)                                                                                                                           ccGTGGGAGAGGGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGTTCCTGCAGGCGCGAAGGATCAGTTCACGGATGGCAGCCCGATCAGGCTGTGGTTGCGCGAAc   >  1:952118/1‑140 (MQ=255)                                                                                                                            cGTGGGAGAGGGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGTTCCTGCAGGCGCGAAGGATCAGTTCACGGATGGCAGCCCGATCAGGCTGTGGTTGCGCGAACa  <  1:1391509/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                  CGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGTTCCTGCAGGCGCGAAGGATCAGTTCACGGATGGCAGCCCGATCAGGCTGTGGTTGCGCGAACA  >  NC_000913/830131‑830393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑