Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	158,295	T→G	T278P (ACC→CCC)	pcnB ←	poly(A) polymerase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	158,295	0	T	G	100.0%	337.9 / NA	96	T278P (ACC→CCC)	pcnB	poly(A) polymerase I
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (51/45);  total (51/45)

AACATGGCGGCAAACAGGAACGCCGGGTTCACGCGCATATCGTTATGGATACGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGTCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTAAGCGATTCTTCAAACAGGCGTGCCGGTGGGATATCGTTCAGCAGGGTAGCGAGGC  >  NC_000913/158162‑158429                                                                                                                                      |                                                                                                                                       aaCATGGCGGCAAACAGGAACGCCGGGTTCACGCGCATATCGTTATGGATACGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGaa                                                                                                                                  >  1:814388/1‑140 (MQ=255)   cATGGCGGCAAACAGGAACGCCGGGTTCACGCGCATATCGTTATGGATACGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACa                                                                                                                                >  1:1052725/1‑140 (MQ=255)   cATGGCGGCAAACAGGAACGCCGGGTTCACGCGCATATCGTTATGGATACGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACa                                                                                                                                >  2:95844/1‑140 (MQ=255)     tGGCGGCAAACAGGAACGCCGGGTTCACGCGCATATCGTTATGGATACGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGc                                                                                                                              >  2:361249/1‑140 (MQ=255)      ggcggcAAACAGGAACGCCGGGTTCACGCGCATATCGTTATGGATACGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCGTTTTCCGTGAACTAGCGGGTAATGGGCGGGAACAGCg                                                                                                                             >  1:548900/1‑140 (MQ=255)      ggcggcAAACAGGAACGCCGGGTTCACGCGCATATCGTTATGGATACGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCg                                                                                                                             >  1:979244/1‑140 (MQ=255)      ggcggcAAACAGGAACGCCGGGTTCACGCGCATATCGTTATGGATACGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCCGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCg                                                                                                                             >  1:90668/1‑140 (MQ=255)       gcggcAAACAGGAACGCCGGGTTCACGCGCATATCGTTATGGATACGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCgg                                                                                                                            >  2:1303685/1‑140 (MQ=255)        cggcAAACAGGAACGCCGGGTTCACGCGCATATCGTTATGGATACGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGTGGGTAATGGGCGGGAACAGCGGc                                                                                                                           <  2:917208/140‑1 (MQ=255)        cggcAAACAGGAACGCCGGGTTCACGCGCATATCGTTATGGATACGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGc                                                                                                                           >  2:1128444/1‑140 (MQ=255)           cAAACAGGAACGCCGGGTTCACACGGATAGCGTTATGGATACGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTgg                                                                                                                        <  1:663375/140‑1 (MQ=255)              aCAGGAACGCCGGGTTCACGCGCATATCGTTATGGATACGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAAc                                                                                                                     <  1:1128444/140‑1 (MQ=255)                  gAACGCCGGGTTCACGCGCATATCGATATTGATACGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAgat                                                                                                                 <  1:727819/140‑1 (MQ=255)                     cGCCGGGTTCACGCGCATATCGTTATGGATACGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAgatgat                                                                                                              <  2:1052725/140‑1 (MQ=255)                       ccGGGTTCACGCGCATATCGTTATGGATACGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGatat                                                                                                            <  2:1291906/140‑1 (MQ=255)                        cGGGTTCACGCGCATATCGTTATGGATACGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATAtt                                                                                                           >  1:1163922/1‑140 (MQ=255)                        cGGGTTCACGCGCATATCGTTATGGATACGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATAtt                                                                                                           >  2:1033132/1‑140 (MQ=255)                        cGGGTTCACGCACATATCGTTATGGATACGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATAtt                                                                                                           >  1:54261/1‑140 (MQ=255)                          ggTTCACGCGCATATCGTTATGGATACGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTca                                                                                                         <  1:361249/140‑1 (MQ=255)                          ggTTCACGCGCATATCGTTATGGATACGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTca                                                                                                         <  1:1303685/140‑1 (MQ=255)                              cACGCGCATATCGTTATGGATACGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTcacaca                                                                                                     <  2:1446923/140‑1 (MQ=255)                               aCGCGCATATCGTTATTGATACGCGTATCGGGAGTCTTCCGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACacaa                                                                                                    <  2:662828/140‑1 (MQ=255)                               aCGCGCATATCGTTATGTATACCCGTATCGGTATTTTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACacaa                                                                                                    <  2:489435/140‑1 (MQ=255)                               aCGCGCATATCGTTATGGATACGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACacaa                                                                                                    <  2:988272/140‑1 (MQ=255)                               aCGCGCATATCGTTATGGATACGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACacaa                                                                                                    <  2:1421232/140‑1 (MQ=255)                                cgcgCATATCGTTATGGATACGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACacaac                                                                                                   <  2:1163922/140‑1 (MQ=255)                                cgcgCATATCGTTATGGATACGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACacaac                                                                                                   <  1:771583/140‑1 (MQ=255)                                             aTGGATACGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGttt                                                                                      >  2:1295844/1‑140 (MQ=255)                                               ggATACGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCg                                                                                    <  1:1033132/140‑1 (MQ=255)                                                 aTACGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTa                                                                                  >  2:1324951/1‑140 (MQ=255)                                                   aCGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGGACAAATGGTATTCACAAAACAACTTATAGGTTTCGTAAc                                                                                >  2:465986/1‑140 (MQ=255)                                                   aCGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATGTTCGCAAAACAGCGTATAGGTTTCGTAAc                                                                                >  1:652027/1‑140 (MQ=255)                                                   aCGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAAc                                                                                >  2:814195/1‑140 (MQ=255)                                                        tATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAgg                                                                                         <  2:1482439/126‑1 (MQ=255)                                                        tATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAgg                                                                                         >  1:1482439/1‑126 (MQ=255)                                                        tATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAg                                                                           <  2:814388/140‑1 (MQ=255)                                                         aTCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGc                                                                          <  2:54261/140‑1 (MQ=255)                                                         aTCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGc                                                                          <  1:1528226/140‑1 (MQ=255)                                                          tCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGcc                                                                         >  2:1511631/1‑140 (MQ=255)                                                          tCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGcc                                                                         >  1:1352972/1‑140 (MQ=255)                                                          tCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGcc                                                                         >  1:1445207/1‑140 (MQ=255)                                                           cGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGccc                                                                        <  1:323329/140‑1 (MQ=255)                                                             gTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGc                                                                      <  2:1041763/140‑1 (MQ=255)                                                               aTTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCtt                                                                    >  1:45953/1‑140 (MQ=255)                                                               aTTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCtt                                                                    >  1:987492/1‑140 (MQ=255)                                                                   ttcAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAg                                                                <  1:810792/140‑1 (MQ=255)                                                                   ttcAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAg                                                                <  1:1144914/140‑1 (MQ=255)                                                                   ttcAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAg                                                                <  1:99976/140‑1 (MQ=255)                                                                     cAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCa                                                              >  2:112727/1‑140 (MQ=255)                                                                     cAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCa                                                              >  1:1252348/1‑140 (MQ=255)                                                                        cACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGGTTATAGGCTTCCTATCCGTAGGCCGCTTGTAGCAGtt                                                           >  1:457877/1‑140 (MQ=255)                                                                        cACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGtt                                                           >  1:854281/1‑140 (MQ=255)                                                                           cTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTTGTATCCCTAGCCCGCTTGTAGCAGTTTaa                                                        >  1:492594/1‑140 (MQ=255)                                                                             gTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTAAGc                                                      >  1:177580/1‑140 (MQ=255)                                                                             gTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTAAGc                                                      >  1:19507/1‑140 (MQ=255)                                                                              ttCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTAAGCg                                                     <  1:917914/140‑1 (MQ=255)                                                                               tCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTAAGCGa                                                    <  1:1511631/140‑1 (MQ=255)                                                                               tCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTAAGCGa                                                    <  1:1295844/140‑1 (MQ=255)                                                                               tCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTAAGCGa                                                    <  2:987492/140‑1 (MQ=255)                                                                                     aTCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTAATAGGTTTGGTAGCCGCAGCCCGCTGGTAGTAGTTTAAGCGAttcttc                                              >  2:614669/1‑140 (MQ=255)                                                                                        cGCTCCATCGGGCTGTCGCCATTTTCGGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGGAGATATTCACACAACCGCTTATGGGTTTCGTCACCGTAGTCCGCTTGTCGCAGATTAAGCGCCTCTTCaaa                                           >  2:736859/1‑140 (MQ=255)                                                                                        cGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAAGCGTAGCCGGCTTGTGGCAGTTAAAGCGATTCTTCaaa                                           >  1:440411/1‑140 (MQ=255)                                                                                        cGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTaa                                                        >  2:1527075/1‑127 (MQ=255)                                                                                        cGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTaa                                                        <  1:1527075/127‑1 (MQ=255)                                                                                        cGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTAAGCGATTCTTCaaa                                           >  2:1288134/1‑140 (MQ=255)                                                                                        cGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTAAGCGATTCTTCaaa                                           >  2:888868/1‑140 (MQ=255)                                                                                        cGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTAAGCGATTCTTCaaa                                           >  1:1503422/1‑140 (MQ=255)                                                                                        cGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTAAGCGATTCTTCaaa                                           >  2:371234/1‑140 (MQ=255)                                                                                        cGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTAAGCGATTCTTCaa                                            >  1:205900/1‑139 (MQ=255)                                                                                        cGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTAAGCGATTCTTCaa                                            <  2:205900/139‑1 (MQ=255)                                                                                         gCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTAAGCGATTCTTCAAAc                                          >  1:1102241/1‑140 (MQ=255)                                                                                           tCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTAAGCGATTCTTCAAACAg                                        >  1:1054592/1‑140 (MQ=255)                                                                                           tCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGGAGCCCGCCTGTGGCGGTTTAAACGATTCTTCAAACAg                                        >  1:566366/1‑140 (MQ=255)                                                                                             cATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTAAGCGATTCTTCAAACAGGc                                      >  1:79592/1‑140 (MQ=255)                                                                                                cGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTAAGCGATTCTTCAAACAGGCGTg                                   >  1:44091/1‑140 (MQ=255)                                                                                                 gggCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTAAGCGATTCTTCAAACAGGCGTGc                                  <  2:1252348/140‑1 (MQ=255)                                                                                                    cTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTAAGCGATTCTTCAAACAGGCGTGCCgg                               >  1:978816/1‑140 (MQ=255)                                                                                                    cTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTAAGCGATTCTTCAAACAGGCGTGCCgg                               <  2:19507/140‑1 (MQ=255)                                                                                                         gCCATTTTCCGTGAAGTCGCGGGTAATGGGCCGGACCAGGGGCTGGAAAAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTAAGCGATTCTTCAAACAGGCGTGCCGGTGGGa                          <  2:566366/140‑1 (MQ=255)                                                                                                         gCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGTAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTAAGCGATTCTTCAAACAGGCGTGCCGGTGGGa                          <  2:1054592/140‑1 (MQ=255)                                                                                                         gCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTAAGCGATTCTTCAAACAGGCGTGCCGGTGGGa                          <  1:371234/140‑1 (MQ=255)                                                                                                         gCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTAAGCGATTCTTCAAACAGGCGTGCCGGTGGGa                          <  1:1324951/140‑1 (MQ=255)                                                                                                         gCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTAAGCGATTCTTCAAACAGGCGTGCCGGTGGGa                          >  2:1443298/1‑140 (MQ=255)                                                                                                         gCCATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTAAGCGATTCTTCAAACAGGCGTGCCGGTGGGa                          >  2:835228/1‑140 (MQ=255)                                                                                                           cATTTTCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTAAGCGATTCTTCAAACAGGCGTGCCGGTGGGata                        <  1:95844/140‑1 (MQ=255)                                                                                                              tttCCGTGAAGTAGCGGTTAATCGGCGGGAAGAGCTGCTTGAACAGATCATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTAAGCGATTCTTCAAACAGGCGTGCCGGTGGGATATCg                     <  2:457877/140‑1 (MQ=255)                                                                                                                tCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTAAGCGATTCTTCAAACAGGCGTGCCGGTGGGATATCGtt                   <  2:207765/140‑1 (MQ=255)                                                                                                                tCCGTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTAAGCGATTCTTCAAACAGGCGTGCCGGTGGGATATCGtt                   <  2:1102241/140‑1 (MQ=255)                                                                                                                   gTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTAAGCGATTCTTCAAACAGGCGTGCCGGTGGGATATCGTTcag                <  2:854281/140‑1 (MQ=255)                                                                                                                   gTGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTAAGCGATTCTTCAAACAGGCGTGCCGGTGGGATATCGTTcag                <  2:1289992/140‑1 (MQ=255)                                                                                                                    tGAAGTAGCGGGTAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTAAGCGGTTCTTCAAACATGCTTGCCGGAGGGATATCGTTcagc               >  1:650896/1‑140 (MQ=255)                                                                                                                                tAATGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTAAGCGATTCTTCAAACAGGCGTGCCGGTGGGATATCGTTCAGCAGGGTAgcgagg   >  1:1153429/1‑140 (MQ=255)                                                                                                                                 aaTGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTAAGCGATTCTTCAAACAGGCGTGCCGGTGGGATATCGTTCAGCAGGGTAgcgaggc  >  2:819623/1‑140 (MQ=255)                                                                                                                                 aaTGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTAAGCGATTCTTCAAACAGGCGTGCCGGTGGGATATCGTTCAGCAGGGTAgcgaggc  <  2:1445207/140‑1 (MQ=255)                                                                                                                                 aaTGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTAAGCGATTCTTCAAACAGGCGTGCCGGTGGGATATCGTTCAGCAGGGTAgc       <  1:1114901/135‑1 (MQ=255)                                                                                                                                 aaTGGGCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTAAGCGATTCTTCAAACAGGCGTGCCGGTGGGATATCGTTCAGCAGGGTAgc       >  2:1114901/1‑135 (MQ=255)                                                                                                                                      |                                                                                                                                       AACATGGCGGCAAACAGGAACGCCGGGTTCACGCGCATATCGTTATGGATACGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGTCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTAAGCGATTCTTCAAACAGGCGTGCCGGTGGGATATCGTTCAGCAGGGTAGCGAGGC  >  NC_000913/158162‑158429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑