Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	935,434	A→G	E737E (GAA→GAG)	ftsK →	cell division DNA translocase FtsK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	935,434	0	A	G	100.0%	203.7 / NA	60	E737E (GAA→GAG)	ftsK	cell division DNA translocase FtsK
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/27);  total (33/27)

AACAACAACGTTATTCCGGCGAACAACCGGCTGGGGCGAATCCGTTCTCGCTGGATGATTTTGAATTTTCGCCAATGAAAGCGTTGCTGGATGATGGTCCACACGAACCGTTGTTTACGCCAATTGTTGAACCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCAGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAA  >  NC_000913/935304‑935569                                                                                                                                   |                                                                                                                                        aacaacaacGTTATTCCGGCGAACAACCGGCTGGGGCGAATCCGTTCTCGCTGGATGATTTTGAATTTTCGCCAATGAAAGCGTTGCTGGATGATGGTCCACACGAACCGTTGTTTACGCCAATTGTTGAGCCTGTAcag                                                                                                                                <  2:1196777/140‑1 (MQ=255)   caacaacGTTATTCCGGCGAACAACCGGCTGGGGCGAATCCGTTCTCGCTGGATGATTTTGAATTTTCGCCAATGAAAGCGTTGCTGGATGATGGTCCACACGAACCGTTGTTTACGCCAATTGTTGAGCCTGTAcagca                                                                                                                              >  1:1296187/1‑140 (MQ=255)   caacaacGTTATTCCGGCGAACAACCGGCTGGGGCGAATCCGTTCTCGCTGGATGATTTTGAATTTTCGCCAATGAAAGCGTTGCTGGATGATGGTCCACACGAACCGTTGTTTACGCCAATTGTTGAGCCTGTAcagca                                                                                                                              <  1:228539/140‑1 (MQ=255)         cGTTATTCCGGCGAACAACCGGCTGGGGCGAATCCGTTCTCGCTGGATGATTTTGAATTTTCGCCAATGAAAGCGTTGCTGGATGATGGTCCACACGAACCGTTGTTTACGCCAATTGTTGAGCCTGTACAGCAGCCGca                                                                                                                        >  1:1441328/1‑140 (MQ=255)         cGTTATTCCGGCGAACAACCGGCTGGGGCGAATCAGTTCTCGCTGGATGATTTTGAATTTTCGCCAATGAAAGCGTTGCTGGATGATGGTCCACACGAACCGTTGTTTACGCCAATTGTTGAGCCTGTACAGCAGCCGca                                                                                                                        >  2:1135099/1‑140 (MQ=255)          gTTATTCCGGCGAACAATCGGCTGGGGCGAATCCGTTCTCGCTGGATGATTTTGAATTTTCGCCAATGAAAGCGTTGCTGGATGATGGTCCACACGAACCGTTGTTTACGCCAATTGTTGAGCCTGTACAGCAGCCGcaa                                                                                                                       <  2:544769/140‑1 (MQ=255)            tATTCCGGCGAACAACCGGCTGGGGCGAATCCGTTCTCGCTGGATGATTTTGAATTTTCGCCAATGAAAGCGTTGCTGGATGATGGTCCACACGAACCGTTGTTTACGCCAATTGTTGAGCCTGTACAGCAGCCGcaaca                                                                                                                     <  2:234158/140‑1 (MQ=255)             aTTCCGGCGAACAACCGGCTGGGGCGAATCCGTTCTCGCTGGATGATTTTGAATTTTCGCCAATGAAAGCGTTGCTGGATGATGGTCCACACGAACCGTTGTTTACGCCAATTGTTGAGCCTGTACAGCAGCCGcaacaa                                                                                                                    <  2:1498561/140‑1 (MQ=255)                  ggCGAACAACCGGCTGGGGCGAATCCGTTCTCGCTGGATGATTTTGAATTTTCGCCAATGAAAGCGTTGCTGGATGATGGTCCACACGAACCGTTGTTTACGCCAATTGTTGAGCCTGTACAGCAGCCGCAACAACCGGt                                                                                                               <  2:1030823/140‑1 (MQ=255)                   gCGAACAACCGGCTGGGGCGAATCCGTTCTCGCTGGATGATTTTGACTTTTCGCCAATGAAAGCGTTGCTGGATGATGGTCCACACGAACCGTTGTTTACGCCAATTGTTGAGCCTGTACAGCAGCCGCAACAACCGGtt                                                                                                              >  1:1458249/1‑140 (MQ=255)                   gCGAACAACCGGCTGGGGCGAATCCGTTCTCGCTGGATGATTTTGAATTTTCGCCAATGAAAGCGTTGCTGGATGATGGTCCACACGAACCCTTGTTTACTCCAGTTGATGAGCCTGGACAGAAGCCGCAACAACCGGtt                                                                                                              >  2:461347/1‑140 (MQ=255)                    cGAACAACCGGCTGGGGCGAATCCGTTCTCGCTGGATGATTTTGAATTTTCGCCAATGAAAGCGTTGCTGGATGATGGTCCACACGAACCGTTGTTTACGCCAATTGTTGAGCCTGTACAGCAGCCGCAACAACCGGTTg                                                                                                             >  2:51018/1‑140 (MQ=255)                      aacaacCGGCTGGGGCGAATCCGTTCTCGCTGGATGATTTTGAATTTTCGCCAATGAAAGCGTTGCTGGATGATGGTCCACACGAACCGTTGTTTACGCCAATTGTTGAGCCTGTACAGCAGCCGCAACAACCGGTTGCa                                                                                                           >  1:1041125/1‑140 (MQ=255)                      aacaacCGGCTGGGGCGAATCCGTTCTCGCTGGATGATTTTGAATTTTCGCCAATGAAAGCGTTGCTGGATGATGGTCCACACGAACCGTTGTTTACGCCAAGTGTTGAGGCTGCACAGCAGCCACAACAACCGGTTGCa                                                                                                           >  1:425223/1‑140 (MQ=255)                       acaacCGGCTGGGGCGAATCCGTTCTCGCTGGATGATTTTGAATTTTCGCCAATGAAAGCGTTGCTGGATGATGGTCCACACGAACCGTTGTTTACGCCAATTGTTGAGCCTGTACAGCAGCCGCAACAACCGGTTGCAc                                                                                                          <  1:209576/140‑1 (MQ=255)                           ccGGCTGGGGCGAATCCGTTCTCGCTGGATGATTTTGAATTTTCGCCAATGAAAGCGTTGCTGGATGATGGTCCACACGAACCGTTGTTTACGCCAATTGTTGAGCCTGTACAGCAGCCGCAACAACCGGTTGCACCgca                                                                                                      >  2:11326/1‑140 (MQ=255)                           ccGGCTGGGGCGAATCCGTTCTCGCTGGATGATTTTGAATTTTCGCCAATGAAAGCGTTGCTGGATGATGGTCCACACGAACCGTTGTTTACGCCAATTGTTGAGCCTGTACAGCAGCCGCAACAACCGGTTGCACCgca                                                                                                      >  2:1076892/1‑140 (MQ=255)                            cGGCTGGGGCGAATCCGTTCTCGCTGGATGATTTTGAATTTTCGCCAATGAAAGCGTTGCTGGATGATGGTCCACACGAACCGTTGTTTACGCCAATTGTTGAGCCTGTACAGCAGCCGCAACAACCGGTTGCACCgcag                                                                                                     <  1:11326/140‑1 (MQ=255)                            cGGCTGGGGCGAATCCGTTCTCGCTGGATGATTTTGAATTTTCGCCAATGAAAGCGTTGCTGGATGATGGTCCACACGAACCGTTGTTTACGCCAATTGTTGAGCCTGTACAGCAGCCGCAACAACCGGTTGCACCgcag                                                                                                     <  1:1076892/140‑1 (MQ=255)                                   ggCGAATCCGTTCTCGCTGGATGATTTTGAATTTTCGCCAATGAAAGCGTTGCTGGATGATGGTCCACACGAACCGTTGTTTACGCCAATTGTTGAGCCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAat                                                                                              >  1:1460707/1‑140 (MQ=255)                                       aaTCCGTTCTCGCTGGATGATTTTGAATTTTCGCCAATGAAAGCGTTGCTGGATGATGGTCCACACGAACCGTTGTTTACGCCAATTGTTGAGCCTGTACAGAAGCCGCACCAAGCGGTTGCACCGCAGCAGCAATATca                                                                                          >  1:701758/1‑140 (MQ=255)                                            gTTCTCGCTGGATGATTTTGAATTTTCGCCAATGAAAGCGTTGCTGGATGATGGTCCACACGAACCGTTGTTTACGCCAATTGTTGAGCCTTTACAGCAGTCGCAACAGCCGGTTGCACCGCAGCAGCAATATCagcagc                                                                                     >  2:554030/1‑140 (MQ=255)                                             ttCTCGCTGGATGATTTTGAATTTTCGCCAATGAAAGCGTTGCTGGATGATGGTCCACACGAACCGTTGTTTACGCCAATTGTTGAGCCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGcc                                                                                    >  2:280342/1‑140 (MQ=255)                                             ttCTCGCTGGATGATTTTGAATTTTCGCCAATGAAAGCGTTGCTGGATGATGGTCCACACGAACCGTTGTTTACGCCAATTGTTGAGCCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGcc                                                                                    >  1:943448/1‑140 (MQ=255)                                              tctcGCTGGATGATTTTGAATTTTCGCCAATGAAAGCGTTGCTGGATGATGGTCCACACGAACCGTTGTTTACGCCAATTGTTGAGCCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATcagcag                                                                                      >  1:378841/1‑137 (MQ=255)                                              tctcGCTGGATGATTTTGAATTTTCGCCAATGAAAGCGTTGCTGGATGATGGTCCACACGAACCGTTGTTTACGCCAATTGTTGAGCCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATcagcag                                                                                      <  2:378841/137‑1 (MQ=255)                                                  gCTGGATGATTTTGAATTTTCGCCAATGAAAGCGTTGCTGGATGATGGTCCACACGAACCGTTGTTTACGCCAATTGTTGAGCCTGTACAGCAGCCGCAACAACCGGTTGGACCACAGCAGCAAGATCAGCAGCCGcaac                                                                               >  1:496268/1‑140 (MQ=255)                                                   cTGGATGATTTTGAATTTTCGCCAATGAAAGCGTTGCTGGATGATGGTCCACACGAACCGTTGTTTACGCCAATTGTTGAGCCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGcaaca                                                                              <  2:18785/140‑1 (MQ=255)                                                      gatgatTTTGAATTTTCGCCAATGAAAGCGTTGCTGGATGATGGTCCACACGAACCGTTGTTTACGCCAATTGTTGAGCCTGTACAGCAGCGGCAACAACTGGTAGCAGCGCAGCAGGAATAGCAGCAGCCGCAACAAcc                                                                           >  2:456679/1‑140 (MQ=255)                                                                aaTTTTCGCCAATGAAAGCGTTGCTGGATGATGGTCCACACGAACCGTTGTTTACGCCAATTGTTGAGCCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTccgccg                                                                 >  2:1017652/1‑140 (MQ=255)                                                                aaTTTTCGCCAATGAAAGCGTTGCTGGATGATGGTCCACACGAACCGTTGTTTACGCCAATTGTTGAGCCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTccgccg                                                                 >  2:390476/1‑140 (MQ=255)                                                                aaTTTTCGCCAATGAAAGCGTTGCTGGATGATGGTCCACACGAACCGTTGTTTACGCCAATTGTTGAGCCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTccgccg                                                                 <  1:1028365/140‑1 (MQ=255)                                                                aaTTTTCGCCAATGAAAGCGTTGCTGGATGATGGTCCACACGAACCGTTGTTTACGCCAATTGTTGAGCCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTccgccg                                                                 <  2:912528/140‑1 (MQ=255)                                                                    ttCGCCAATGAAAGCGTTGCTGGATGATGGTCCACACGAACCGTTGTTTACGCCAATTGTTGAGCCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCgccgcagc                                                             <  2:1041125/140‑1 (MQ=255)                                                                    ttCGCCAATGAAAGCGTTGCTGGATGATGGTCCACACGAACCGTTGTTTACGCCAATTGTTGAGCCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCgccgcagc                                                             <  1:390476/140‑1 (MQ=255)                                                                    ttCGCCAATGAAAGCATTGCTGCATCATGGTCCACACGAACCGTTGTTTACGCCAATTGTTGAGCCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCgccgcagc                                                             <  1:654758/140‑1 (MQ=255)                                                                     tCGCCAATGAAAGCGTTGCTGGATGATGGTCCACACGAACCGTTGTTTACGCCAATTGTTGAGCCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCcgccgc                                                                <  1:284775/136‑1 (MQ=255)                                                                     tCGCCAATGAAAGCGTTGCTGGATGATGGTCCACACGAACCGTTGTTTACGCCAATTGTTGAGCCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCcgccgc                                                                >  2:284775/1‑136 (MQ=255)                                                                     tCGCCAATGAAAGCGTTGCTGGATGATGGTCCACACGAACCGTTGTTTACGCCAATTGTTGAGCCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCgccgcagcc                                                            >  2:1427978/1‑140 (MQ=255)                                                                     tCGCCAATGAAAGCGTTGCTGGATGATGGTCCACACGAACCGTTGTTTACGCCAATTGTTGAGCCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCgccgcagcc                                                            <  2:1441328/140‑1 (MQ=255)                                                                         cAATGAAAGCGTTGCTGGATGATGGTCCACACGAACCGTTGTTTACGCCAATTGTTGAGCCTGTACAGCAGCCGCATCAACCGGTTGCACCGCAGCAGAAATATCAGCAGCCGCAACAGCCAGTTCCGccgcagccgcag                                                        <  1:1135099/140‑1 (MQ=255)                                                                             gAAAGCGTTGCTGGATGATGGTCCACACGAACCGTTGTTTACGCCAATTGTTGAGCCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCAGCCGCAGTATc                                                    <  2:943448/140‑1 (MQ=255)                                                                              aaaGCGTTGCTGGATGAGGGTCAACACGAACAGTTTTTTCCGCCAATTTTTGAGCCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCAGCCGCAGTATca                                                   <  2:425223/140‑1 (MQ=25)                                                                                    ttGCTGGATGATGGTCCACACGAACCGTTGTTTACGCCAATTGTTGAGCCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCAGCCGCAGTATca                                                   <  1:306685/134‑1 (MQ=255)                                                                                    ttGCTGGATGATGGTCCACACGAACCGTTGTTTACGCCAATTGTTGAGCCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCAGCCGCAGTATca                                                   >  2:306685/1‑134 (MQ=255)                                                                                     tGCTGGATGATGGTCCACACGAACCGTTGTTTACGCCAATTGTTGAGCCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCAGCCGCAGTATCAGCAGCca                                            <  1:1248870/140‑1 (MQ=255)                                                                                             gatgGTCCACACGAACCTTTGTGTACGCCATCTGTTGAGACTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGTCGCAGCCGCAGTATCAGCAGCCACAACAGcc                                    <  2:496268/140‑1 (MQ=18)                                                                                               tgGTCCACACGAACCGTTGTTTACGCCAATTGTTGAGCCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCAGCCGCAGTATCAGCAGCCACAACAGCCgg                                  >  1:1153809/1‑140 (MQ=255)                                                                                                 gTCCACACGAACCGTTGTTTACGCCAATTGTTGAGCCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCAGCCGCAGTATCAGCAGCCACAACAGCCGGtt                                >  2:1190998/1‑140 (MQ=255)                                                                                                 gTCCACACGAACCGTTGTTTACGCCAATTGTTGAGCCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCAGCCGCAGTATCAGCAGCCACAACAGCCGGtt                                >  1:1222753/1‑140 (MQ=255)                                                                                                    cacaCGAACCGTTGTTTACGCCAATTGTTGAGCCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCAGCCGCAGTGTCTGCAGCCTCAACAGCCGGTTgcg                             >  1:657758/1‑140 (MQ=255)                                                                                                         gAACCGTTGTTTACGCCAATTGTTGAGCCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCAGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCa                        <  1:280342/140‑1 (MQ=255)                                                                                                           aCCGTTGTTTACGCCAATTGTTGAGCCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCAGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGc                      >  2:322166/1‑140 (MQ=255)                                                                                                           aCCGTTGTTTACGCCAATTGTTGAGCCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCAGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGc                      >  2:1502234/1‑140 (MQ=255)                                                                                                            ccGTTGTTTACGCCAATTGTTGCGCCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCAGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGcc                     <  1:461347/140‑1 (MQ=255)                                                                                                            ccGTGGTTTACGCCATTGGTGGGCCAGGTACAGCAGCCGCTGCAACGGTGTGCACCGCAGCGGCAATATAAGCCGCCCCAGCAACCAGTGCGGCCGCAGCCGCGGTATAAGCAGCCACAACAGCCGGTTGCGCCGCAGcc                     <  2:1458249/140‑1 (MQ=255)                                                                                                                       gCCAATTGTTGAGCCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCAGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATcagc          >  2:384104/1‑140 (MQ=255)                                                                                                                        ccAATTGTTGAGCCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCAGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATcagca         <  1:1427978/140‑1 (MQ=255)                                                                                                                            ttgttgAGCCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTGCGCCGCAGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAACCACAATATCAGCAGCCg     >  2:1520481/1‑140 (MQ=255)                                                                                                                            ttgttgAGCCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCAGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCg     >  1:126408/1‑140 (MQ=255)                                                                                                                               ttgAGCCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCAGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGcaa  >  1:71553/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                        AACAACAACGTTATTCCGGCGAACAACCGGCTGGGGCGAATCCGTTCTCGCTGGATGATTTTGAATTTTCGCCAATGAAAGCGTTGCTGGATGATGGTCCACACGAACCGTTGTTTACGCCAATTGTTGAACCTGTACAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCAGTTCCGCCGCAGCCGCAGTATCAGCAGCCACAACAGCCGGTTGCGCCGCAGCCACAATATCAGCAGCCGCAA  >  NC_000913/935304‑935569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑