Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,010,898 C→T A312V (GCC→GTC)  uup → ATP‑binding protein with possible role in replication

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,010,8980CT97.4% 116.5 / NA 38A312V (GCC→GTC) uupATP‑binding protein with possible role in replication
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (22/15);  minor base G (1/0);  total (23/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAA  >  NC_000913/1010767‑1011024
                                                                                                                                   |                                                                                                                              
gtgGATCCGCCAGGGGATCAAATCACGCCGTCCCCGTAAGGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCt                                                                                                                        <  2:528575/140‑1 (MQ=255)
   gATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCg                                                                                                                     >  1:390786/1‑140 (MQ=255)
      ccGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTa                                                                                                                  >  1:1321982/1‑140 (MQ=255)
                     aGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGaaa                                                                                                   <  2:390786/140‑1 (MQ=255)
                         cGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGa                                                                                               >  1:864753/1‑140 (MQ=255)
                         cGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGa                                                                                               >  2:832477/1‑140 (MQ=255)
                          gCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAATAGGTCAGCCGCTCCGGTAAAATCGTTTTCGaaa                                                                                                   >  2:785738/1‑135 (MQ=255)
                          gCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAATAGGTCAGCCGCTCCGGTAAAATCGTTTTCGaaa                                                                                                   <  1:785738/135‑1 (MQ=255)
                               aCCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGt                                                                                         >  1:244128/1‑140 (MQ=255)
                                 ccGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGttt                                                                                       <  1:1148345/140‑1 (MQ=255)
                                           ggCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGt                                                                             >  2:158127/1‑140 (MQ=255)
                                           ggCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGt                                                                             <  1:158127/140‑1 (MQ=255)
                                           ggCCGCGTACGCGCCCTAAAAGCGATGCGTCGCGACCGTTGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGt                                                                             <  2:564429/140‑1 (MQ=255)
                                                cgTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTATCAGGTTAACg                                                                        >  1:805718/1‑140 (MQ=255)
                                                cgTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGt                                                                             <  2:1133704/135‑1 (MQ=255)
                                                cgTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCCCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGt                                                                             >  1:1133704/1‑135 (MQ=255)
                                                             aaaGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTGTGCAACAAGGTTAGCGGTAAGCAACTGGt                                                           >  1:444478/1‑140 (MQ=255)
                                                                   aTGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTAACAGATTGACGGTAGGCAACTGGTGAAAGa                                                     >  1:515060/1‑140 (MQ=255)
                                                                       gTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGAtttt                                                 >  1:1435485/1‑140 (MQ=255)
                                                                                  ggtgAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGcc                                           >  2:97955/1‑135 (MQ=255)
                                                                                  ggtgAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGcc                                           <  1:97955/135‑1 (MQ=255)
                                                                                   gtgAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGtt                                     >  1:884447/1‑140 (MQ=255)
                                                                                                   gATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACaaaa                     <  2:244128/140‑1 (MQ=255)
                                                                                                        gTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGcc                <  2:1321982/140‑1 (MQ=255)
                                                                                                        gTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGcc                <  2:884447/140‑1 (MQ=255)
                                                                                                          aCCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCt              >  1:993948/1‑140 (MQ=255)
                                                                                                            cGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAAtt                   >  2:1481165/1‑133 (MQ=255)
                                                                                                            cGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAAtt                   <  1:1481165/133‑1 (MQ=255)
                                                                                                            cGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGa            >  1:1093400/1‑140 (MQ=255)
                                                                                                            cGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGa            >  1:1444739/1‑140 (MQ=255)
                                                                                                            cGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGa            >  1:56835/1‑140 (MQ=255)
                                                                                                             gCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGAt           <  2:846439/140‑1 (MQ=255)
                                                                                                               aaaGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTg         <  2:1093400/140‑1 (MQ=255)
                                                                                                                 aGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGt       <  2:864753/140‑1 (MQ=255)
                                                                                                                   aTGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTcc     >  1:284680/1‑140 (MQ=255)
                                                                                                                   aTGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTcc     >  2:27956/1‑140 (MQ=255)
                                                                                                                      cAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGaa  >  1:876954/1‑140 (MQ=255)
                                                                                                                      cAGGTGGAAGAGGGCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGaa  >  1:77372/1‑140 (MQ=255)
                                                                                                                                   |                                                                                                                              
GTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAA  >  NC_000913/1010767‑1011024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑