Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,019,811	A→G	R81R (CGT→CGC)	ompA ←	outer membrane porin A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,019,811	0	A	G	98.8%	286.0 / NA	85	R81R (CGT→CGC)	ompA	outer membrane porin A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (44/40);  minor base T (1/0);  total (45/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGCACGCCATACCATGCCACCCAGACGAGTGTAGATGTCCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTGTATGCACCGTTTTCAACGCTGCCTTTGTACGGCATACGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTCATGGGTCGGGCCATTGTTGTTGATGAAACCAGTGTCATGGTACTGGGACC  >  NC_000913/1019678‑1019947                                                                                                                                      |                                                                                                                                         cTGCACGCCATACCATGCCACCCAGACGAGTGTAGATGTCCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTGTATGCACCGTTTTCAACGCTGCCTTTGTACGGCATGCGACCt                                                                                                                                    >  2:322746/1‑140 (MQ=255)  tGCACGCCATACCATGCCACCCAGACGAGTGTAGATGTCCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTGTATGCACCGTTTTCAACGCTGCCTTTGTACGGCATGCGACCTa                                                                                                                                   >  1:1535907/1‑140 (MQ=255)    cACGCCATACCATGCCACCCAGACGAGTGTAGATGTCCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTGTATGCACCGTTTTCAACGCTGCCTTTGTACGGCATGCGACCTAAc                                                                                                                                 <  1:312216/140‑1 (MQ=255)    cACGCCATACCATGCCACCCAGACGAGTGTAGATGTCCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTGTATGCACCGTTTTCAACGCTGCCTTTGTACGGCATGCGACCTAAc                                                                                                                                 <  1:116639/140‑1 (MQ=255)          aTACCATGCCACCCAGACGAGTGTAGATGTCCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTGTATGCACCGTTTTCAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCg                                                                                                                           <  1:1333746/140‑1 (MQ=255)            aCCATGCCACCCAGACGAGTGTAGATGTCCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTGTATGAACCGTTTTTAACGCTGGCTTTGTACGGCATGCGACCTAACCAGTCGTa                                                                                                                         >  2:404405/1‑140 (MQ=255)                 gCCACCCAGACGAGTGTAGATGTCCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTGTATGCACCGTTTTCAACGGTGCTTTTGTACGGCATTCGACATAACCAGTCGTAACCCa                                                                                                                    >  2:447269/1‑140 (MQ=255)                 gCCACCCAGACGAGTGTAGATGTCCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTGTATGCACCGTTTTCAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCa                                                                                                                    >  2:161795/1‑140 (MQ=255)                 gCCACCCAGACGAGTGTAGATGTCCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTGTATGCACCGTTTTCAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCa                                                                                                                    >  1:295508/1‑140 (MQ=255)                 gCCACCCAGACGAGTGTAGATGTCCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTGTATGCACCGTTTTCAACGCTCCCTTTGTCCGGCATGCGACCTCACCAGTCGTAACCCa                                                                                                                    >  1:661938/1‑140 (MQ=255)                   cACCCAGACGAGTGTAGATGTCCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTGTATGCACCGTTTTCAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCAtt                                                                                                                  >  2:1099273/1‑140 (MQ=255)                   cACCCAGACGAGTGTAGATGTCCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTGTATGCACCGTTTTCAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCAtt                                                                                                                  >  1:965377/1‑140 (MQ=255)                     cccAGACGAGTGTAGATGTCCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTGTATGCACCGTTTTCAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTc                                                                                                                >  2:1102623/1‑140 (MQ=255)                     cccAGACGAGTGTAGATGTCCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTGTATGCACCGTTTTCAACGCTGCCTATGTACGGCATGGGACCTAACCGGTCGTAACCCATTTc                                                                                                                >  1:508875/1‑140 (MQ=255)                      ccAGACGAGTGTAGATGTCCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTGTATGCACCGTTTTCAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCa                                                                                                               <  1:1096490/140‑1 (MQ=255)                       cAGACGAGTGTAGATGTCCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTGTATGCACCGTTTTCAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCaa                                                                                                              <  1:258288/140‑1 (MQ=255)                             aGTGTAGATGTCCACGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTGTATGCACCGTTTTCAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCaa                                                                                                        >  1:250993/1‑140 (MQ=255)                               tgtAGATGTCCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTGTATGCACCGTTTTCAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACa                                                                                                      >  2:1462398/1‑140 (MQ=255)                               tgtAGATGTCCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTGTATGCACCGTTTTCAACGCTGCCTTTGTACGGCAAGCGACCTAACCGGTAGTAACCAATTTCAAAGCCAACa                                                                                                      >  1:503472/1‑140 (MQ=255)                               tgtAGATGTCCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTGTATGCACCGTTTTCAACGCTGCCATTGTACGGGATGCGACCTAACCAGTTGTAAGCCATTTCAAAGCCAACa                                                                                                      >  2:484119/1‑140 (MQ=255)                                gtAGATGTCCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTGTATGCACCGTTTTCAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACAt                                                                                                     <  2:307999/140‑1 (MQ=255)                                gtAGATGTCCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTGTATGCACCGTTTTCAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACAt                                                                                                     <  2:965377/140‑1 (MQ=255)                                gtAGATGTCCACGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTGTATGCACCGTTTTCAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACAt                                                                                                     <  2:250993/140‑1 (MQ=255)                                   gATGTCCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTGTATGCACCGTTTTCAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACg                                                                                                  <  2:1535907/140‑1 (MQ=255)                                    aTGTCCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTGTATGCACCGTTTTCAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACgg                                                                                                 >  2:848170/1‑140 (MQ=255)                                       tCCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTGTATGCACCGTTTTCAACGCTGCCTTTGTACGTCATGCGACCTAAGCAGTCGTAACCCGTTTCGAAGCCAACATACGGGtt                                                                                              >  2:416312/1‑140 (MQ=255)                                       tCCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTGTATGCACCGTTTTCAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGtt                                                                                              >  2:1365040/1‑140 (MQ=255)                                        ccAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTGTATGCACCGTTTTCAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATa                                                                                                    >  2:234904/1‑133 (MQ=255)                                        ccAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTGTATGCACCGTTTTCAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATa                                                                                                    <  1:234904/133‑1 (MQ=255)                                        ccAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTGTATGCACCGTTTTCAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTa                                                                                             >  1:368301/1‑140 (MQ=255)                                         cAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTGTATGCACCGTTTTCAACGCTGCCTTTGTACGGCTTGCGACCTCACCGGTCCTAACCCATCTCAAGGCCAACATACGGGTTaa                                                                                            >  2:611510/1‑140 (MQ=255)                                         cAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTGTATGCACCGTTTTCAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTaa                                                                                            >  2:1397149/1‑140 (MQ=255)                                              cgtcAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTGTATGCACCGTTTTCAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATa                                                                                                    >  1:1176962/1‑127 (MQ=255)                                              cgtcAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTGTATGCACCGTTTTCAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATa                                                                                                    <  2:1176962/127‑1 (MQ=255)                                                 cAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTGTATGCACCGTTTTCAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTaa                                                                                    <  2:295508/140‑1 (MQ=255)                                                                     ttAGCGGTCAGTTGAACGCCCTGAGCTTTGTATGCACCGTTTTCAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGcc                                                                >  1:100542/1‑140 (MQ=255)                                                                     ttAGCGGTCAGTTGAACGCCCTGAGCTTTGTATGCACCGTTTTCAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGcc                                                                >  1:1129528/1‑140 (MQ=255)                                                                      tAGCGGTCAGTTGAACGCCCTGAGCTTTGTATGCACCGTTTTCAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGccc                                                               >  2:908404/1‑140 (MQ=255)                                                                        gCGGTCAGTTGAACGCCCTGAGCTTTGTATGCACCGTTTTCAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACGTGGTAAGCACCAACAGCACCAGCGCCCAg                                                             >  1:482625/1‑140 (MQ=255)                                                                          ggTCAGTTGAACGCCCTGAGCTTTGTATGCACCGTTTTCAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAgcg                                                                  >  1:1037898/1‑133 (MQ=255)                                                                          ggTCAGTTGAACGCCCTGAGCTTTGTATGCACCGTTTTCAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAgcg                                                                  <  2:1037898/133‑1 (MQ=255)                                                                             cAGTTGAACGCCCTGAGCTTTGTATGCACCGTTTTCAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTgg                                                         >  2:1480134/1‑139 (MQ=255)                                                                             cAGTTGAACGCCCTGAGCTTTGTATGCACCGTTTTCAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTgg                                                         <  1:1480134/139‑1 (MQ=255)                                                                             cAGTTGAACGCCCTGAGCTTTGTATGCACCGTTTTCAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGt                                                        >  2:962784/1‑140 (MQ=255)                                                                             cAGTTGAACGCCCTGAGCTTTGTATGCACCGTTTTCAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGt                                                        >  2:877423/1‑140 (MQ=255)                                                                              aGTTGAACGCCCTGAGCTTTGTATGCACCGTTTTCAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGtt                                                       >  1:25507/1‑140 (MQ=255)                                                                                  gAACGCCCTGAGCTTAGTATGCCCCCTTTCCAACGCTTCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTCa                                                   <  1:447269/140‑1 (MQ=255)                                                                                   aaCGCCCTGAGCTTTGTATGCACCGTTTTCAACGCTGCCTTTGTACGGCATGCGACCTAACCGGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTCAt                                                  >  2:891796/1‑140 (MQ=255)                                                                                       cccTGAGCTTTGTATGCACCGTTTTTAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTCATGGGt                                              <  1:1274249/140‑1 (MQ=255)                                                                                       cccTGAGCTTTGTATGCACCGTTTTCAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTCATGGGt                                              <  1:1365040/140‑1 (MQ=255)                                                                                         cTGAGCTTTGTATGCACCGTTTTCAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTCATGGGTCg                                            <  1:908404/140‑1 (MQ=255)                                                                                          tGAGCTTTGTATGCACCGTTTTCAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCAGCAAAAGCGCCAGCGACCAGTTGGTTTTCATGGGTCgg                                           >  1:431442/1‑140 (MQ=255)                                                                                          tGAGCTTTGTATGCACCGTTTTCAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTCATGGGTCgg                                           >  2:766384/1‑140 (MQ=255)                                                                                              cTTTGTATGCACCGTTTTCAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTCATGGGTCGGGCCa                                       <  1:877423/140‑1 (MQ=255)                                                                                              cTTTGTATGCACCGTTTTCAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTCATGGGTCGGGCCa                                       <  1:848170/140‑1 (MQ=255)                                                                                                 tGTATGCACCGTTTTCAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTCATGGGTCGGGCCAttg                                    >  1:1139108/1‑140 (MQ=255)                                                                                                 tGTATGCACCGTTTTCAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTCATGGGTCGGGCCAttg                                    >  1:1113142/1‑140 (MQ=255)                                                                                                    aTGCACCGTTTTCAATGCTGCCTTTATACGGCATGCGGCCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTCATGGATCGGGCCAttgttg                                 <  1:641294/140‑1 (MQ=255)                                                                                                    aTGCACCGTTTTCAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTCATGGGTCGGGCCAttgttg                                 <  1:322746/140‑1 (MQ=255)                                                                                                           gTTTTCAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTCATGGGTCGGGCCATTGTTGTtgatga                          >  2:97164/1‑140 (MQ=255)                                                                                                           gTTTTCAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTCATGGGTCGGGCCATTGTTGTtgatga                          >  2:251287/1‑140 (MQ=255)                                                                                                            ttttCAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCCGTTGGTTTTCAGGGGTCGGGCCATTGTTGTTGATGaa                         >  1:581644/1‑140 (MQ=255)                                                                                                            ttttCAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTCATGGGTCGGGCCATTGTTGTTGATGaa                         >  1:1293289/1‑140 (MQ=255)                                                                                                              ttCAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTCATGGGTCGGGCCATTGTTGTTGATGAAAc                       <  2:100542/140‑1 (MQ=255)                                                                                                              ttCAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTCATGGGTCGGGCCATTGTTGTTGATGAAAc                       <  2:1129528/140‑1 (MQ=255)                                                                                                               tCAACGCTGCCTTTGTATGGCAGGCGACCTACCCATTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTCATGGGTCGGGCCATTGTTGTTGATGAAAcc                      <  2:539975/140‑1 (MQ=255)                                                                                                               tCAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTCATGGGTCGGGCCATTGTTGTTGATGAAAcc                      <  2:1233578/140‑1 (MQ=255)                                                                                                                cAACGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTCATGGGTCGGGCCATTGTTGTT‑ATGAAACCAg                    <  2:1139108/140‑1 (MQ=255)                                                                                                                 aaCGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTCATGGGTCGGGCCAttgttg                                 <  1:1091706/127‑1 (MQ=255)                                                                                                                 aaCGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTCATGGGTCGGGCCAttgttg                                 >  2:1091706/1‑127 (MQ=255)                                                                                                                 aaCGCTGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTCATGGGTCGGGCCATTGTTGTTGATGAAACCAg                    <  2:1113142/140‑1 (MQ=255)                                                                                                                 aaCGCTGCCTTTGTACGGCATGCGACCTAACAAGTCGTATCCCATTTCCAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTCATGGGTCGGGCCATTGTTGTTGATGAAACCAg                    <  2:431442/140‑1 (MQ=255)                                                                                                                      tGCCTTTGTACGGCATGCGTCCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTCATGGGTCGGGCCATTGTTGTTGATGAAACCAGTGTCa               <  2:1293289/140‑1 (MQ=255)                                                                                                                      tGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTCATGGGTCGGGCCATTGTTGTTGATGAAACCAgt                   <  1:1144941/136‑1 (MQ=255)                                                                                                                      tGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTCATGGGTCGGGCCATTGTTGTTGATGAAACCAgt                   >  2:1144941/1‑136 (MQ=255)                                                                                                                      tGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTCATGGGTCGGGCCATTGTTGTTGATGAAACCAGTGTCa               <  1:1462398/140‑1 (MQ=255)                                                                                                                      tGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTCATGGGTCGGGCCATTGTTGTTGATGAAACCAGTGTCa               <  1:1099273/140‑1 (MQ=255)                                                                                                                      tGCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTCATGGGTCGGGCCATTGTTGTTGATGAAACCAGTGTCa               >  2:149360/1‑140 (MQ=255)                                                                                                                       gCCTTTGTACGGCATGCGACCTGACAAGTTGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTCATGGGTCGGGCCATTGTTGTTGATGAAACCAGTGTCAt              <  1:484119/140‑1 (MQ=255)                                                                                                                       gCCTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTCATGGGTCGGGCCATTGTTGTTGATGAAACCAGTGTCAt              <  1:251287/140‑1 (MQ=255)                                                                                                                        ccTTTGTACGGCATGTGACCTACCCCGTCATAACCCACTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTCATGGGTCGGGCCATTGTTGTTGATGAAACCAGTGTCATg             <  1:404405/140‑1 (MQ=255)                                                                                                                        ccTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTCATGGGTCGGGCCATTGTTGTTGATGAAACCAGTGTCATg             <  1:1102623/140‑1 (MQ=255)                                                                                                                        ccTTTGTACGGCATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTCATGGGTCGGGCCATTGTTGTTGATGAAACCAGTGTCATg             <  1:97164/140‑1 (MQ=255)                                                                                                                             gTACGGCATGCGACCCAACCAGTCGCAACCCATTTCAGAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTCATGGGTCGGGCCATTGTTGTTGATGAAACCAGTGTCATGGTACt        <  1:611510/140‑1 (MQ=255)                                                                                                                                   cATGCGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTCATGGGTCGGGCCATTGTTGTTGATGAAACCAGTGTCATGGTACTGGGAcc  >  2:1124550/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                         CTGCACGCCATACCATGCCACCCAGACGAGTGTAGATGTCCAGGTCGTCAGTGATTGGGTAACCCAGTTTAGCGGTCAGTTGAACGCCCTGAGCTTTGTATGCACCGTTTTCAACGCTGCCTTTGTACGGCATACGACCTAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTCATGGGTCGGGCCATTGTTGTTGATGAAACCAGTGTCATGGTACTGGGACC  >  NC_000913/1019678‑1019947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑