Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,056,094 A→G Y29H (TAC→CAC)  torS ← sensory histidine kinase TorS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,056,0940AG98.0% 167.6 / NA 49Y29H (TAC→CAC) torSsensory histidine kinase TorS
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (22/26);  minor base T (0/1);  total (22/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGGCACTGGTCAGGTTCTGCGCGGCGAAAAGTTCCCAGGCGCTGGCTTCGCTCAACTGGCGCGCCATATTCATGGTAGGAATCAATGCCTGAGTGTTGTCTTTTTCCACCTGGCTGATAAAGCGCAGGTTGTACCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAGCCCATCCAGAGTCTTCGGGTCAGGGTTAAATTCACGGTCGGTGCACTTTAGGTGAAAAAGGTTGAGTCGCAAAGCGGAATGCATC  >  NC_000913/1055961‑1056228
                                                                                                                                     |                                                                                                                                      
tCGGCACTGGTCAGGTTCTGCGCGGCGAAAAGTTCCCAGGCGCTGGCTTCGCTCAACTGGCGCGCCATATTCATGGTAGGAATCAATGCCTGAGTGTTGTCTTTTTCCACCTGGCTGATAAAGCGCAGGTTGTGCCATcc                                                                                                                                  <  2:1030285/140‑1 (MQ=255)
  ggCACTGGTCAGGTTCTGCGCGGCGAAAAGTTCCCAGGCGCTGGCTTCGCTCAACTGGCGCGCCATATTCATGGTAGGAATCAATGCCTGAGTGTTGTCTTTTTCCACCTGGCTGATAAAGCGCAGGTTGTGCCATCcca                                                                                                                                >  1:1229739/1‑140 (MQ=255)
     aCTGGTCAGGTTCTGCGCGGCGAAAAGTTCCCAGGCGCTGGCTTCGCTCAACTGGCGCGCCATATTCATGGTAGGAATCAATGCCTGAGTGTTGTCTTTTTCCACCTGGCTGATAAAGCGCAGGTTGTGCCATCccacca                                                                                                                             <  1:111475/140‑1 (MQ=255)
          tCAGGTTCTGCGCGGCGAAAAGTTCCCAGGCGCTGGCTTCGCTCAACTGGCGCGCCATATTCATGGTAGGAATCAATGCCTGAGTGTTGTCTTTTTCCACCTGGCTGATAAAGCGCAGGTTGTGCCATCCCACCAGGGTa                                                                                                                        <  1:1039794/140‑1 (MQ=255)
          tCAGGTTCTGCGCGGCGAAAAGTTCCCAGGCGCTGGCTTCGCTCAACTGGCGCGCCATATTCATGGTAGGAATCAATGCCTGAGTGTTGTCTTTTTCCACCTGGCTGATAAAGCGCAGGTTGTGCCATCCCACCAGGGTa                                                                                                                        <  2:848774/140‑1 (MQ=255)
              gTTCTGCGCGGCGAACAGTTCCCAGCCGCTGGCTTCGCTCAACTGGCGCGCCATATTCATGGTAGGAATCAATGCCTGAGTGTTGTCTTTTTCCACCTGGCTGATAAAGCGCAGGTTGTGCCATCCCACCAGGGTACTgg                                                                                                                    <  1:426088/140‑1 (MQ=255)
              gTTCTGCGCGGCGAAAACTTCCCAGGCGCTGTCTTTGCTCAACTGGCGCGCCATATTCATGGTAGGAATCAATGCCTGAGTGTTGTCTTTTTCCACCTGGCTGATAAAGCGCAGGTTGTGCCATCCCACCAGGGTACTgg                                                                                                                    <  2:542855/140‑1 (MQ=255)
                   gcgcGGCGAAAAGTTCCCAGGCGCTGGCTTCGCTCAACTGGCGCGCCATATTCATGGTAGGAATCAATGCCTGAGTGTTGTCTTTTTCCACCTGGCTGATAAAGCGCAGGTTGTGCCATCCCACCAGGGTACTGGTCAgg                                                                                                               <  2:780391/140‑1 (MQ=255)
                        gcgAAAAGTTCCCAGGCGCTGGCTTCGCTCAACTGGCGCGCCATATTCATGGTAGGAATCAATGCCTGAGTGTTGTCTTTTTCCACCTGGCTGATAAAGCGCAGGTTGTGCCATGCCGCCAGGGCACTGGTCAGGGTTaa                                                                                                          >  1:722799/1‑140 (MQ=255)
                        gcgAAAAGTTCCCAGGCGCTGGCTTCGCTCAACTGGCGCGCCATATTCATGGTAGGAATCAATGCCTGAGTGTTGTCTTTTTCCACCTGGCAGATAAAGCACAGATTGCGCCATCCCGCCAGCGTACTGGTCAGGGTTaa                                                                                                          >  2:426088/1‑140 (MQ=255)
                              aGTTCCCAGGCGCTGGCTTCGCTCAACTGGCGCGCCATATTCATGGTAGGAATCAATGCCTGAGTGTTGTCTTTTTCCACCTGGCTGATAAAGCGCAGGTTGTGCCATCCCACCAGGGTACTGGTCAGGGTTAACAgcgc                                                                                                    <  2:212239/140‑1 (MQ=255)
                                          cTGGCTTCGCTCAACTGGCGCGCCATATTCATGGTAGGAATCAATGCCTGAGTGTTGTCTTTTTCCACCTGGCTGATAAAGCGCAGGTTGTGCCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCaaa                                                                                        >  2:772795/1‑140 (MQ=255)
                                          cTGGCTTCGCTCAACTGGCGCGCCATATTCATGGTAGGAATCAATGCCTGAGTGTTGTCTTTTTCCACCTGGCTGATAAAGCGCAGGTTGTGCCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCaaa                                                                                        >  2:1051698/1‑140 (MQ=255)
                                            ggCTTCGCTCAACTGGCGCGCCA‑ATTCATGGTAGGAATCAATGCCTGAGTGTTGTCTTTTTCCACCTGGCTGATAAAGCGCAGGTTGTGCCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAGcc                                                                                     <  2:770443/140‑1 (MQ=255)
                                                   cTCAACTGGCGCGACATGATCACGGTAGGAAACAAGGCCCGAGTGTTGTCTTTTTCCACCTGGCTGATAAAGCGCAGGTTGTGCCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAGCCCATCCa                                                                               <  2:722799/140‑1 (MQ=255)
                                                     cAACTGGCGCGCCATCTTCATGTTATGAACCAATGCCTGAGTGTTGTCTTTTTCCACCTGGCTGATAAAGCGCAGGTTGTGCCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAGCCCATCCaga                                                                             <  1:703822/140‑1 (MQ=255)
                                                     cAACTGGCGCGCCATATTCATGGTAGGAATCAATGCCTGAGTGTTGTCTTTTTCCACCTGGCTGATAAAGCGCAGGTTGTGCCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAGCCCATCCaga                                                                             <  2:1229739/140‑1 (MQ=255)
                                                           gcgcgcCATATTCATGGTAGGAATCAATGCCTGAGTGTTGTCTTTTTCCACCTGGCTGATAAAGCGCAGGTTGTGCCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAGCCCAt                                                                                  >  2:232731/1‑129 (MQ=255)
                                                           gcgcgcCATATTCATGGTAGGAATCAATGCCTGAGTGTTGTCTTTTTCCACCTGGCTGATAAAGCGCAGGTTGTGCCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAGCCCAt                                                                                  <  1:232731/129‑1 (MQ=255)
                                                               gcCATATTCATGGTAGGAATCAATGCCTGAGTGTTGTCTTTTTCCACCTGGCTGATAAAGCGCAGGTTGTGCCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAGCCCATCCAGAGTCTTCGGGt                                                                   <  1:1039975/140‑1 (MQ=255)
                                                                 cATATTCATGGTAGGAATCAATGCCTGAGTGTTGTCTTTTTCCACCTGGCTGATAAAGCGCAGGTTGTGCCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAGCCCATCCAGAGTCTTCGGGTCa                                                                 <  1:129431/140‑1 (MQ=255)
                                                                             aGGAATCAATGCCTGAGTGTTGTCTTTTTCCACCTGGCTGATAAAGCGCAGGTTGTGCCATCCCACCAGGGTACTGGTCAGGGTTAACAGCTCCATCAGGCCAATGCCAATCCAGAGTCTTCAGGTCAGGGTTAAATTCa                                                     >  2:475006/1‑140 (MQ=255)
                                                                             aGGAATCAATGCCTGAGTGTTGTCTTTTTCCACCTGGCTGATAAAGCGCAGGTTGTGCCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAGCCCAACCAGAGGCTTCGGGTCAGGGTTAAATTCa                                                     >  1:493172/1‑140 (MQ=255)
                                                                               gAATCAATGCCTGAGTGTTGTCTTTTTCCACCTGGCTGATAAAGCGCAGGTTGTGCCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAGCCCATCCAGAGTCTTCGGGTCAGGGTTAAATTCAcg                                                   >  1:48918/1‑140 (MQ=255)
                                                                               gAATCAATGCCTGAGTGTTGTCTTTTTCCACCTGGCTGATAAAGCGCAGGTTGTGCCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAGCCCATCCAGAGTCTTCGGGTCAGGGTTAAATTCAcg                                                   <  1:382420/140‑1 (MQ=255)
                                                                                 aTCAATGCCTGAGTGTTTTCTTTTTCCACCTTGCTGATACAGCGCAGGTTGTGCCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAGCCCATCCAGAGTCTTCGGGTCAGGGTTAAATTCAcggt                                                 <  2:493779/140‑1 (MQ=255)
                                                                                    aaTGCCTGAGTGTTGTCTTTTTCCACCTGGCTGATAAAGCGCAGGTTGTGCCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAGCCCATCCAGAGTCTTCGGGTCAGGGTTAAATTCAcggtcgg                                              <  2:254430/140‑1 (MQ=255)
                                                                                    aaTGCCTGAGTGTTGTCTTTTTCCACCTGGCTGATAAAGCGCAGGTTGTGCCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAGCCCATCCAGAGTCTTCGGGTCAGGGTTAAATTCAcggtcgg                                              <  2:145873/140‑1 (MQ=255)
                                                                                      tGCCTGAGTGTTGTCTTTTTCCACCTGGCTGATAAAGCGCAGGTTGTGCCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAGCCCATCCAGAGTCTTCGGGTCAGGGTTAAATTCACGGTCGGTg                                            <  2:48918/140‑1 (MQ=255)
                                                                                          tGAGTGTTGTCTTTTTCCACCTGGCTGATAAAGCGCAGGTTGTGCCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAGCCCATCCAGAGTCTTCGAGTCAGGGTTAAATTCACGGTCGGTGCACt                                        >  1:493779/1‑140 (MQ=255)
                                                                                            aGTGTTGTCTTTTTCCACCTGGCTGATAAAGCGCAGGTTGTGCCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAGCCCATCCAGAGTCTTCGGGTCAGGGTTAAATTCACGGTCGGTGCACttt                                      >  1:1431559/1‑140 (MQ=255)
                                                                                              tgttgtCTTTTTCCACCTGGCTGATAAAGCGCAGGTTGTGCCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAGCCCATCCAGAGGCTTGGGGGCAGGGTTAAATTCACGGTCGGTGCACTTTAg                                    >  2:534821/1‑140 (MQ=255)
                                                                                              tgttgtCTTTTTCCACCTGGCTCATAAAGCGTAGGCTGTTCCATCCCATCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAGCCCATCCAGAGTCTTCGGGTCAGGGTTAAATTCACGGTCGGTGCACTTTAg                                    <  2:481734/140‑1 (MQ=255)
                                                                                                ttgtCTTTTTCCACCTGGCTGATAAAGCGCAGGTTGTGCCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAGCCCATCCAGAGTCTTCGGGTCAGGGTTAAATTCACGGTCGGTGCACTTTAGGt                                  >  2:103265/1‑140 (MQ=255)
                                                                                                   tCTTTTTCCACCTGGCTGATAAAGCGCAGGTTGTGCCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAGCCCATCCAGAGTCTTCGGGTCAGGGCTAAATTCAcggt                                                 <  1:867925/122‑1 (MQ=255)
                                                                                                   tCTTTTTCCACCTGGCTGATAAAGCGCAGGTTGTGCCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAGCCCATCCAGAGTCTTCGGGTCAGGGCTAAATTCAcggt                                                 >  2:867925/1‑122 (MQ=255)
                                                                                                               tGGCTGATAAAGCGCAGGTTGTGCCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAGCCCATCCAGAGTCTTCGGGTCAGGGTTAAATTCACGGTCGGTGCACTTTAGGTGAAAAAGGTTGAGTc                   >  2:1373128/1‑140 (MQ=255)
                                                                                                                 gCTGATAAAGCGCAGGTTGTGCCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAGCCCATCCAGAGTCTTCGGGTCAGGGTTAAATTCACGGTCGGTGCACTTTAGGTGAAAAAGGTTGAGTCGc                 >  2:1279431/1‑140 (MQ=255)
                                                                                                                  cTGATAAAGCGCAGGTTGTGCCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAGCCCATCCAGAGTCTTCGGGTCAGGGTTAAATTCACGGTCGGTGCACTTTAGGTGAAAAAGGTTGAGTCGCa                <  2:1213400/140‑1 (MQ=255)
                                                                                                                     aTAAAGCGCAGGTTGTGCCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAGCCCATCCAGAGTCTTCGGGTCAGGGTTAAATTCACGGTCGGTGCACTTTAGGTGAAAAAGGTTGAGTCGCAAAg             <  1:1373128/140‑1 (MQ=255)
                                                                                                                     aTAAAGCGCAGGTTGTGCCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAGCCCATCCAGAGTCGTCGGGTCAGGGTTAAATTCACGGTCGGTGCACTTTAGGTGAAAAAGGTTGAGTCGCAAAg             >  2:896267/1‑140 (MQ=255)
                                                                                                                          gcgcAGGTTGTGCCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAGCCCATCCAGAGTCTTCGGGTCAGGGTTAAATTCACGGTCGGTGCACTTTAGGTGAAAAAGGTTGAGTCGCAAAGCGGaa        <  1:1279431/140‑1 (MQ=255)
                                                                                                                           cgcAGGTTGTGCCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAGCCCATCCAGAGTCTTCGGGTCAGGGTTAAATTCACGGTCGGTGCACTTTAGGTGAAAAAGGTTGAGTCGCAAAGCGGAAt       >  2:1061428/1‑140 (MQ=255)
                                                                                                                           cgcAGGTTGTGCCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAGCCCATCCAGAGTCTTCGGGTCAGGGTTAAATTCACGGTCGGTGCACTTTAGGTGAAAAAGGTTGAGTCGCAAAGCGGAAt       <  1:896267/140‑1 (MQ=255)
                                                                                                                           cgcAGGTTGTGCCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAGCCCATCCAGAGTCTTCGGGTCAGGGTTAAATTCACGGTCGGTGCACTTTAGGTGAAAAAGGTTGAGTCGCAAAGCGGAAt       >  1:277633/1‑140 (MQ=255)
                                                                                                                           cgcAGGTTGTGCCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAGCCCATCCAGAGTCTTCGGGTCAGGGTTAAATTCACGGTCGGTGCACTTTAGGTGAAAAAGGTTGAGTCGCAAAGCGGAAt       >  1:1338125/1‑140 (MQ=255)
                                                                                                                           cgcAGGTTGTGCCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAGCCCATCCAGAGTCTTCGGGTCAGGGTTAAATTCACGGTCGGTGCACTTTAGGTGAAAAAGGTTGAGTCGCAAAGCGGAAt       >  1:101335/1‑140 (MQ=255)
                                                                                                                           cgcAGGTTGTGCCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAGCCCATCCAGAGTCTTCGGGTCAGGGTTAAATACACGGTCGATGCACTTGAGGTGAAACAGGTCGAGTCGCAAAGCGGAAt       >  2:602023/1‑140 (MQ=255)
                                                                                                                             cAGGTTGTGCCATCCTACCAGGCTAATGGCCAGGGTTCACAGCGCCATCAGGGCAAAGCCCATCCAGAGTCTTCGGGTCAGGGTTAAATTCACGGTCGGTGCACTTTAGGTGAAAAAGGTTGAGTCGCAAAGCGGAATGc     <  1:475006/140‑1 (MQ=255)
                                                                                                                                gTTGTGCCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAGCCCATCCAGAGTCTTCGGGTCAGGGTTAAATTCACGGTCGGTGCACTTTAGGTGAAAAAGGTTGAGTCGCAAAGCGGAATGCATc  >  2:136730/1‑140 (MQ=255)
                                                                                                                                     |                                                                                                                                      
TCGGCACTGGTCAGGTTCTGCGCGGCGAAAAGTTCCCAGGCGCTGGCTTCGCTCAACTGGCGCGCCATATTCATGGTAGGAATCAATGCCTGAGTGTTGTCTTTTTCCACCTGGCTGATAAAGCGCAGGTTGTACCATCCCACCAGGGTACTGGTCAGGGTTAACAGCGCCATCAGGGCAAAGCCCATCCAGAGTCTTCGGGTCAGGGTTAAATTCACGGTCGGTGCACTTTAGGTGAAAAAGGTTGAGTCGCAAAGCGGAATGCATC  >  NC_000913/1055961‑1056228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑