Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,249,873	T→C	S322S (TCA→TCG)	dhaK ←	dihydroxyacetone kinase subunit K

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,249,873	0	T	C	100.0%	289.8 / NA	82	S322S (TCA→TCG)	dhaK	dihydroxyacetone kinase subunit K
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (43/39);  total (43/39)

TGAGTTCTGCTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGACCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGTGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCA  >  NC_000913/1249738‑1249997                                                                                                                                        |                                                                                                                             tGAGTTCTGCTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGACCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAgg                                                                                                                          >  2:887285/1‑140 (MQ=255)  gAGTTCTGCTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGACCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGt                                                                                                                         >  1:1260669/1‑140 (MQ=255)  gAGTTCTGCTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGACCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGt                                                                                                                         >  1:953473/1‑140 (MQ=255)        tGCTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGACCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAAATTTCAGTAAGGTCAATGAGAAACCGATCATACCCAGCGATGTGCAGTa                                                                                                                   >  2:691548/1‑140 (MQ=255)          cTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGACCGGGGCGTCCCAGAGTGCCATCGTTTCGCCATCAACTTGCAGTAAGTTGAGTGAGAAACCGGGCATATGCAGCGTGGTGCatgacg                                                                                                                 >  1:954198/1‑135 (MQ=255)          cTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGACCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTAcg                                                                                                                 >  2:941446/1‑140 (MQ=255)           tCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGACCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTAcgc                                                                                                                >  1:1468869/1‑140 (MQ=255)             aGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGACCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACgcgc                                                                                                              <  2:1468869/140‑1 (MQ=255)                    ttGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGACCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAATCGGTCATATCCGGCAAGGTGCGGTACGCGCCaattaa                                                                                                       >  1:589630/1‑140 (MQ=255)                         ttCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGACCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTAc                                                                                                  >  2:1544226/1‑140 (MQ=255)                          tctcCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGACCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACg                                                                                                 >  2:1192676/1‑140 (MQ=255)                           ctcCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGACCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGCGATTGAGAAACCGGTCAGATCCAGCGAGGGGCAGTACGCGCCAATTAAATTACGt                                                                                                >  1:422486/1‑140 (MQ=255)                              cTTATTTACCCCAGTTAAGGGCCGGGGTGTGGACCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCg                                                                                             <  2:133023/140‑1 (MQ=255)                              cTTATTTACCCCAGTTAAGGGCCGGGGTGTGGACCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCg                                                                                             <  1:1544226/140‑1 (MQ=255)                                 aTTTACCCCAGTTAAGGGCCGGGGTGTGGACCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATa                                                                                          >  1:1049643/1‑140 (MQ=255)                                   ttACCCCAGTTAAGGGCCGGGGTGTGGACCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGt                                                                                        <  1:1192676/140‑1 (MQ=255)                                      ccccAGTTAAGGGCCGGGGTGTGGACCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCaa                                                                                     >  2:1419957/1‑140 (MQ=255)                                      ccccAGTTAAGGGCCGGGGTGTGGACCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCaa                                                                                     >  2:204257/1‑140 (MQ=255)                                      ccccAGTTAAGGGCCGGGGTGTGGACCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCaa                                                                                     >  2:1159222/1‑140 (MQ=255)                                      ccccAGTTAAGGGCCGGGGTGTGGACCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCaa                                                                                     >  2:819853/1‑140 (MQ=255)                                        ccAGTTAAGGGCAGGGGTGTGGACCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATc                                                                                   >  2:78234/1‑140 (MQ=255)                                           gTTAAGGGCCGGGGTGTGGACCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCg                                                                                >  2:787057/1‑140 (MQ=255)                                            ttAAGGGCCGGGGTGTGGACCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGc                                                                               >  1:1073689/1‑140 (MQ=255)                                            ttAAGGGCCGGGGTGTGGACCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGc                                                                               >  1:1111915/1‑140 (MQ=255)                                               aGGGCCGGGGTGTGGACCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTg                                                                            <  1:941446/140‑1 (MQ=255)                                                    cGGGGTGTGGACCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGc                                                                       >  1:93839/1‑140 (MQ=255)                                                    cGGGGTGTGGACCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGc                                                                       >  1:1288962/1‑140 (MQ=255)                                                    cGGGGTGTGGACCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGc                                                                       <  2:1111915/140‑1 (MQ=255)                                                      gggTGTGGACCGGGGCGTCCTAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCaa                                                                     >  2:326544/1‑140 (MQ=255)                                                      gggTGTGGACCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCaa                                                                     >  2:83077/1‑140 (MQ=255)                                                      gggTGTGGACCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCaa                                                                     >  1:1258916/1‑140 (MQ=255)                                                      gggTGTGGACCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCaa                                                                     >  2:62905/1‑140 (MQ=255)                                                      gggTGTGGACCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCaa                                                                     >  2:808740/1‑140 (MQ=255)                                                      gggTGGGGACCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAACTAAATTAAGTTAGAAAGTCAATCCCGCTTGCTGGCaa                                                                     >  1:569979/1‑140 (MQ=255)                                                           tgGACCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACgtgt                                                                >  2:1160784/1‑140 (MQ=255)                                                              aCCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTAAGTTCGATAGTCGATCCCGCTTCCTGGCAACGTGTGGt                                                             >  1:608880/1‑140 (MQ=255)                                                                cGGGGCGTCCCAGAGTGCCAGTGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCa                                                           >  1:361487/1‑140 (MQ=255)                                                                cGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCa                                                           <  1:819853/140‑1 (MQ=255)                                                                cGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCa                                                           <  1:808740/140‑1 (MQ=255)                                                                cGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCa                                                           <  1:78234/140‑1 (MQ=255)                                                                  gggCGTCCCAGAGTGCCTGCGTCTCGTCATCGACTGTCAATAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAgg                                                         <  2:555805/140‑1 (MQ=255)                                                                  gggCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCGGCTTGCTGGCAACGTGTGGTCAgg                                                         <  1:204257/140‑1 (MQ=255)                                                                  gggCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAgg                                                         <  1:83077/140‑1 (MQ=255)                                                                  gggCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAgg                                                         >  1:280413/1‑140 (MQ=255)                                                                  gggCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAgg                                                         >  1:187390/1‑140 (MQ=255)                                                                  gggCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAgg                                                         >  2:768421/1‑140 (MQ=255)                                                                  gggCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAgg                                                         >  1:1044793/1‑140 (MQ=255)                                                                  gggCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAgg                                                         <  2:1261693/140‑1 (MQ=255)                                                                  gggCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAgg                                                         <  1:62905/140‑1 (MQ=255)                                                                  gggCGTCCCAGAGTGACAGCGTATCGTCAGCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAgg                                                         <  1:691548/140‑1 (MQ=255)                                                                   ggCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCa                                                           >  1:389634/1‑137 (MQ=255)                                                                   ggCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCa                                                           <  2:389634/137‑1 (MQ=255)                                                                    gCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCg                                                       <  1:887285/140‑1 (MQ=255)                                                                    gCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCg                                                       <  2:1073689/140‑1 (MQ=255)                                                                     cGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCgg                                                      <  1:787057/140‑1 (MQ=255)                                                                         ccAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTtat                                                  <  1:1419957/140‑1 (MQ=255)                                                                         ccAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTtat                                                  <  2:178401/140‑1 (MQ=255)                                                                              gTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACg                                             <  2:174149/140‑1 (MQ=255)                                                                                 ccAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTtat                                                  >  2:944934/1‑132 (MQ=255)                                                                                 ccAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTtat                                                  <  1:944934/132‑1 (MQ=255)                                                                                 ccAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCg                                          >  1:153765/1‑140 (MQ=255)                                                                                 ccAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCg                                          >  2:83857/1‑140 (MQ=255)                                                                                 ccAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCg                                          >  2:1088370/1‑140 (MQ=255)                                                                                   agtgTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTa                                        <  2:361487/137‑1 (MQ=255)                                                                                   aGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTa                                        <  1:83857/140‑1 (MQ=255)                                                                                   aGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTa                                        <  2:1049643/140‑1 (MQ=255)                                                                                         tCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTc                                  >  1:126242/1‑140 (MQ=255)                                                                                                   cTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGa                        >  2:53618/1‑140 (MQ=255)                                                                                                    tttCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGTGGTTATAGACGCCGTACAGCTCAGAAAGCGGAg                       <  1:1159222/140‑1 (MQ=255)                                                                                                    tttCAGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAg                       >  1:1251429/1‑140 (MQ=255)                                                                                                        aGTAAGGTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTgc                   <  1:1160784/140‑1 (MQ=255)                                                                                                             ggTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGTGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCaa              <  2:1186523/140‑1 (MQ=255)                                                                                                             ggTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCaa              <  2:1251429/140‑1 (MQ=255)                                                                                                             ggTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCaa              <  2:126242/140‑1 (MQ=255)                                                                                                             ggTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCaa              <  1:768421/140‑1 (MQ=255)                                                                                                             ggTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCaa              <  2:187390/140‑1 (MQ=255)                                                                                                             ggTGATTGAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCaa              <  1:326544/140‑1 (MQ=255)                                                                                                             ggTGATTGAGAAACCGGTCATACCCAGCGAGGTGCCGTACGCGCCAATAAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCaa              <  2:608880/140‑1 (MQ=255)                                                                                                             ggTGATTAAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCaa              >  2:814280/1‑140 (MQ=255)                                                                                                             ggTGATTAAGAAACCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCaa              <  1:814280/140‑1 (MQ=255)                                                                                                                       aaaCCGGTCATATCCAGCGAGGTGCAGTACGCGCCGATTCAATTACGTCCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAAc    <  2:422486/140‑1 (MQ=255)                                                                                                                         aCCGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCa  <  2:1288962/140‑1 (MQ=255)                                                                                                                           cGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCaa              <  1:1354422/126‑1 (MQ=255)                                                                                                                           cGGTCATATCCAGCGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAACTCAGAAAGCGGAGTTGCGCCaa              >  2:1354422/1‑126 (MQ=255)                                                                                                                                        |                                                                                                                             TGAGTTCTGCTCAGTGACATTGCTTTCTCCTTATTTACCCCAGTTAAGGGCCGGGGTGTGGACCGGGGCGTCCCAGAGTGCCAGCGTTTCGTCATCAACTTTCAGTAAGGTGATTGAGAAACCGGTCATATCCAGTGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCA  >  NC_000913/1249738‑1249997

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑