Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,295,065	C→T	pseudogene (258/897 nt)	insZ ←	putative transposase InsZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,295,065	0	C	T	100.0%	156.1 / NA	47	pseudogene (258/897 nt)	insZ	putative transposase InsZ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/28);  total (19/28)

TCAATGGCAAAAAGTTGCAGGCCGTGCCAGTCATCCTTCAGGTAACGTTCCGCGCCCCTGTCTGTGCGGTCTGGCGGAAGAGCCATTCCACTGGGGCGGCCCCCACGCGCTGACGCGCCTGGGTGACAGCGCTGCGGGCCAGCAGGTTCATCCCCGCTTCGCCATCCGCGCTCAGGTTCAGACGGCGAACAACATCGGTAATTGGCTCATTGCACCACCATCCAGATAACCATGTCCCCCGGTAAACGACGGCGGCGAACGGT  >  NC_000913/1294931‑1295193                                                                                                                                       |                                                                                                                                 tCAATGGCAAAAAGTTGCAGGCCGTGCCAGTCATCCTTCAGGTAACGTTCCGCGCCCCTGTCTGTGCGGTCTGGCGGAAGAGCCATTCCACTGGGGCGGCCCCCACGCGCTGACGCGCCTGGGTGACAGCGC‑GTGGGCca                                                                                                                            >  1:165206/1‑140 (MQ=255)   aaTGGCAAAAAGTTGCAGGCCGTGCCAGTCATCCTTCAGGTAACGTTCCGCGCCCCTGTCTGTGCGGTCTGGCGGAAGAGCCATTCCACTGGGGCGGCCCCCACGCGCTGACGCGCCTGGGTGACAGCGC‑GTGGGCcagc                                                                                                                          <  2:174664/140‑1 (MQ=255)   aaTGGCAAAAAGTTGCAGGCCGTGCAAGTCATCCTTCTGGTAACGTTCCGCGCCCCTGTCTGTGCGGTCTGGCGGAAGAGCCATTCCACTGGGGCGGCCCCCACGCGCTGACGCGCCTGGGTGACAGCGC‑GTGGGCcagc                                                                                                                          <  1:409164/140‑1 (MQ=255)    aTGGCAAAAAGTTGCAGGCCGTGCCAGTCATCCTTCAGGTAACGTTCCGCGCCCCTGTCTGTGCGGTCTGGCGGAAGAGCCATTCCACTGGGGCGGCCCCCACGCGCTGACGCGCCTGGGTGACAGCGC‑GTGGGCcagca                                                                                                                         >  2:182437/1‑140 (MQ=255)               ttGCAGGCCGTGCCAGTCATCCTTCAGGTAACGTTCCGCGCCCCTGTCTGTGCGGTCTGGCGGAAGAGCCATTCCACTGGGGCGGCCCCCACGCGCTGACGCGCCTGGGTGACAGCGC‑GTGGGCCAGCAGGTTCATcccc                                                                                                              <  1:182437/140‑1 (MQ=255)                         tGCCAGTCATCCTTCAGGTAACCTTCCGCACCCCTGTCTGTGCGGTCTGGCGGAAGAGCCATTCCACTGGGGCGGCCCCCACGCGCTGACGCGCCTGGGTGACAGCGC‑GTGGGCCAGCAGGTTCATCCCCGCTTCGCCAt                                                                                                    <  1:504337/140‑1 (MQ=255)                               tCATCCTTCAGGTAACGTTCCGCGCCCCTGTCTGTGCGGTCTGGCGGAAGAGCCATTCCACTGGGGCGGCCCCCACGCGCTGACGCGCCTGAGGGACACCTC‑GTAGGCTAGCAGGTTCATCCGCGCTTCGCCACCCgcgc                                                                                              >  2:504337/1‑140 (MQ=255)                                cATCCTTCAGGTAACGTTCCGCGCCCCTGTCTGTGCGGTCTGGCGGAAGAGCCATTCCACTGGGGCGGCCCCCACGCGCTGACGCGCCTGGGTGACAGCGC‑GTGGGCCAGCAGGTTCATCCCCGCTTCGCCATCCGCGCt                                                                                             <  1:1322604/140‑1 (MQ=255)                                cATCCTTCAGGTAACGTTCCGCGCCCCTGTCTGTGCGGTCTGGCGGAAGAGCCATTCCACTGGGGCGGCCCCCACGCGCTGACGCGCCTGGGTGACAGCGC‑GTGGGCCAGCAGGTTCATCCCCGCTTCGCCATCCGCGCt                                                                                             <  1:932008/140‑1 (MQ=255)                                          gTAACGTTCCGCGCCCCTGTCTGTGCGGTCTGGCGGAAGAGCCATTCCACTGGGGCGGCCCCCACGCGCTGACGCGCCTGGGTGACAGCGC‑GTGGGCCAGCAGGTTCATCCCCGCTTCGCCATCCGCGCTCAGGTTCAGa                                                                                   <  2:1404654/140‑1 (MQ=255)                                             aCGTTCCGCGCCCCTGTCTGTGCGGTCTGGCGGAAGAGCCATTCCACTGGGGCGGCCCCCACGCGCTGACGCGCCTGGGTGACAGCGC‑GTGGGCCAGCAGGTTCTTCCACGCTTCGCTATCCGTGCTCAGGTTCAGACgg                                                                                >  2:699596/1‑140 (MQ=255)                                             aCGTTCCGCGCCCCTGTCTGTGCGGTCTGGCGGAAGAGCCATTCCACTGGGGCGGCCCCCACGCGCTGACGCGCCTGGGTGACAGCGC‑GTGGGCCAGCAGATTCATCCCCGCTTCGCAATCCCCGCTCAGGTTCAGACgg                                                                                >  2:561856/1‑140 (MQ=255)                                                    gcgcCCCTGTCTGTGCGGTCTGGCGGAAGAGCCATTCCACTGGGGCGGCCCCCACGCGCTGACGCGCCTGGGTGACAGCGC‑GTGGGCCAGCAGGTTCATCCCCGCTTCGCCATCCGCGCTCAGGTTCAGACGGCGaacaa                                                                         >  1:205051/1‑140 (MQ=255)                                                    gcgcCCCTGTCTGTGCGGTCTGGCGGAAGAGCCATTCCACTGGGGCGGCCCCCACGCGCTGACGCGCCTGGGTGACAGCGC‑GTGGGCCAGCAGGTTCATCCCCGCTTCGCCATCCGCGCTCAGGTTCAGACGGCGaacaa                                                                         <  1:1501008/140‑1 (MQ=255)                                                                  gCGGTCTGGCGGAAGAGCCATTCCACTGGGGCGGCCCCCACGCGCTGACGCGCCTGGGTGACAGCGC‑GTGGGCCAGCAGGTTCATCCCCGCTTCGCCATCCGCGCTCAGGTTCAGACGGCGAACAACATCGGTAATTGGc                                                           >  1:1431598/1‑140 (MQ=255)                                                                  gCGGTCTGGCGGAAGAGCCATTCCACTGGGGCGGCCCCCACGCGCTGACGCGCCTGGGTGACAGCGC‑GTGGGCCAGCAGGTTCATCCCCGCTTCGCCATCCGCGCTCAGGTTCAGACGGCGAACAACATCGGTAATTGGc                                                           >  1:1385332/1‑140 (MQ=255)                                                                  gCGGTCTGGCGGAAGAGCCATTCCACTGGGGCGGCCCCCACGCGCTGACGCGCCTGGGTGACAGCGC‑GTGGGCCAGCAGGTTCATCCCCGCTTCGCCATCCGCGCTCAGGTTCAGACGGCGAACAACATCGGTAATTGGc                                                           >  1:628007/1‑140 (MQ=255)                                                                  gCGGTCTGGCGGAAGAGCCATTCCACTGGGGCGGCCCCCACGCGCTGACGCGCCTGGGTGACAGCGC‑GTGGGCCAGCAGGTTCATCCCCGCTTCGCCATCCGCGCTCAGGTTCAGACCGCGAACTACATCGGTAATTGGc                                                           >  1:685200/1‑140 (MQ=255)                                                                  gCGGTCTGGCGGAAGAGCCATTCCACTGGGGCGGCCCCCACGCGCTGACGCGCCTGGGTGACAGCGC‑GTGGGCCAGCAGGTTCATCCCCGCCTCGCCATCCGCGTTCATGTTCAGACTGCGAACAACATCGGTAATTGGc                                                           >  2:562807/1‑140 (MQ=255)                                                                   cGGTCTGGCGGAAGAGCCATTCCACTGGGGCGGCCCCCACGCGCTGACGCGCCTGGGTGACAGCGC‑GTGGGCCAGCAGGTTCATCCCCGCTTCGCCATCCGCGCTCAGGTTCAGACGGCGAACAACATCGGTAATTGGCt                                                          <  1:830897/140‑1 (MQ=255)                                                                   cGGTCTGGCGGAAGAACCATTCCACTGGGGCGGCCCCGACGCGCTGACGCGCCTGGGTGACAGCGC‑GTGGGCCAGCAGGTTCATCCCCGCTTCGCCATCCGCGCTCAGGTTCAGACGGCGAACAACATCGGTAATTGGCt                                                          <  1:508990/140‑1 (MQ=255)                                                                      tCTGGCGGAAGAGCCATTCCACTGGGGCGGCCCCCACGCGCTGACGCGCCTGGGTGACAGCGC‑GTGGGCCAGCAGGTTCATCCCCGCTTCGCCATCCGCGCTCAGGTTCAGACGGCGAACAACATCGGTAATTGGCTCAt                                                       >  2:469/1‑140 (MQ=255)                                                                      tCTGGCGGAAGAGCCATTCCACTGGGGCGGCCCCCACGCGCTGACGCGCCTGGGTGACAGCGC‑GTGGGCCAGCAGGTTCATCCCCGCTTCGCCATCCGCGCTCAGGTTCAGACGGCGAACAACATCGGTAATTGGCTCAt                                                       >  1:77194/1‑140 (MQ=255)                                                                       cTGGCGGAAGAGCCATTCCACTGGGGCGGCCCCCACGCGCTGACGCGCCTGGGTGACAGCGC‑GTGGGCCAGCAGGTTCATCCCCGCTTCGCGATCCGCGCCCAGGTACAGACGGCGAACAACAGCGGTAATGGGCTCAtt                                                      >  2:695268/1‑140 (MQ=255)                                                                        tGGCGGAAGAGCCATTCCACTGAGGCGGCTCCCACGCTCTGACGCGCCTGGGTGACAGCGC‑GTGGGCCAGCAGGTTCATCCCCGCTTCGCCATCCGCGCTCAGGTTCAGACGGCGAACAACATCGGTAATTGGCTCATTg                                                     <  1:403613/140‑1 (MQ=255)                                                                           cGGAAGAGCCATTCCACTGGGGCGGCCCCCACGCGCTGACGCGCCTGGGTGACAGCGC‑GTGGGCCAGCAGGTTCATCCCCGCTTCGCCATCCGCGCTCAGGTTCAGACGGCGAACAACATCGGTAATTGGCTCATTGcac                                                  <  1:1257999/140‑1 (MQ=255)                                                                             gAAGAGCCATTCCACTGGGGCGGCCCCCACGCGCTGACGCGCCTGGGTGACAGCGC‑GTGGGCCAGCAGGTTCATCCCCGCTTCGCCATCCGCGCTCAGGTTCAGACGGCGAACAACATCGGTAATTGGCTCATTGcacca                                                <  2:205051/140‑1 (MQ=255)                                                                               agagCCATTCCACTGGGTCGGCCCCCACGCGCTGACGCGCCTGGGTGATAGCGC‑GTGGGCCAGCAGGTTCATCCCCGCTTCGCCATCCGCGCTCAGGTTCAGACGGCGAACAACATCGGTAATTGGCTCATTGCaccacc                                              <  2:418598/140‑1 (MQ=255)                                                                               agagCCATTCCACTGGGGCGGCCCCCACGCGCTGACGCGCCTGGGTGACAGCGC‑GTGGGCCAGCAGGTTCATCCCCGCTTCGCCATCCGCGCTCAGGTTCAGACGGCGAACAACATCGGTAATTGGCTCATTGCaccacc                                              <  2:1031665/140‑1 (MQ=255)                                                                                gagCCATTCCACTGGGGCGGCCCCCACGCGCTGACGCGCCTGGGTGACAGCGC‑GTGGGCCAGCAGGTTCATCCCCGCTTCGCCATCCGCGCTCAGGTTCAGACGGCGAACAACATCGGTAATTGGCTCATTGCAccacca                                             <  2:628007/140‑1 (MQ=255)                                                                                gagCCATTCCACTGGGCGGGCCCCCACGCGCCGCCTCGCTTGGGTGACTGCGC‑GTGGGCCAGCAGGTTCATCCCCGCTTCGCCATCCGCGCTCAGGTTCAGACGGCGAACAACATCGGTAATTGGCTCATTGCAccacca                                             <  2:685200/140‑1 (MQ=255)                                                                                 agCCATTCCACTGGGGCGGCCCCCACGCGCTGACGCGCCTGGGTGACAGCGC‑GTGGGCCAGCAGGTTCATCCCCGCTTCGCCATCCGCGCTCAGGTTCAGACGGCGAACAACATCGGTAATTGGCTCATTGCACCACCAt                                            <  1:1522005/140‑1 (MQ=255)                                                                                 agCCATTCCACTGGGGCGGCCCCCACGCGCTGACGCGCCTGGGTGACAGCGC‑GTGGGCCAGCAGGTTCATCCCCGCTTCGCCATCCGCGCTCAGGTTCAGACGGCGAACAACATCGGTAATTGGCTCATTGCACCACCAt                                            <  1:469/140‑1 (MQ=255)                                                                                  gCCATTCCACTGGGGCGGCCCCCACGCGCTGACGCGCCTGGGTGACAGCGC‑GTGGGCCAGCAGGTTCATCCCCGCTTCGCCATCCGCGCTCAGGTTCAGACGGCGAACAACATCGGTAATTGGCTCATTGcac                                                  >  2:818260/1‑133 (MQ=255)                                                                                  gCCATTCCACTGGGGCGGCCCCCACGCGCTGACGCGCCTGGGTGACAGCGC‑GTGGGCCAGCAGGTTCATCCCCGCTTCGCCATCCGCGCTCAGGTTCAGACGGCGAACAACATCGGTAATTGGCTCATTGcac                                                  <  1:818260/133‑1 (MQ=255)                                                                                      ttCCACTGGGGCGGCGCCCACGCGCGGACGCGCCTGGGTGACAGCGC‑GTGGGCCAGCAGGTTCATCCCCGCTTCGCCATCCGCGCTCAGGTTCAGACGGCGAACAACATCGGTAATTGGCTCATTGCACCACCATCCAGa                                       <  1:699596/140‑1 (MQ=255)                                                                                      ttCCACTGGGGCGGCCCCCACGCGCTGACGCGCCTGGGTGACAGCGC‑GTGGGCCAGCAGGTTCATCCCCGCTTCGCCATCCGCGCTCAGGTTCAGACGGCGAACAACATCGGTAATTGGCTCATTGCACCACCATCCAGa                                       <  1:359312/140‑1 (MQ=255)                                                                                      ttCCACTGGGGCGGCCCCCACGAGCTGACGCGCCTGGTTGACAGCGC‑GTGGGCCAGCAGGTTCATCCCCGCTTCGCCATCCGCGCTCAGGTTCAGACGGCGAACAACATCGGTAATTGGCTCATTGCACCACCATCCAGa                                       <  1:561856/140‑1 (MQ=255)                                                                                               ggcggcCCCCACGCGCTGACGCGCCTGGGTGACAGCGC‑GTGGGCCAGCAGGTTCATCCCCGCTTCGCCATCCGCGCTCAGGTTCAGACGGCGAACAACATCGGTAATTGGCTCATTGCaccacc                                              >  1:1521045/1‑124 (MQ=255)                                                                                               ggcggcCCCCACGCGCTGACGCGCCTGGGTGACAGCGC‑GTGGGCCAGCAGGTTCATCCCCGCTTCGCCATCCGCGCTCAGGTTCAGACGGCGAACAACATCGGTAATTGGCTCATTGCaccacc                                              <  2:1521045/124‑1 (MQ=255)                                                                                                 cggcCCCCACGCGCTGACGCGCCTGGGTGACAGCGC‑GTGGGCCAGCAGGTTCATCCCCGCTTCGCCATCCGCGCTCAGGTTCAGACGGCGAACAACATCGGTAATTGGCTCATTGCACCACCATCCAGATAACCATGTcc                            >  1:1338115/1‑140 (MQ=255)                                                                                                  ggcCCCCACGCGCTGACGCGCCTGGGTGACAGCGC‑GTGGGCCAGCAGGTTCATCCCCGCTTCGCCATCCGCGCTCAGGTTCAGACGGCGAACAACATCGGTAATTGGCTCATTGCACCACCATCCAGATAACCAt                                >  2:292008/1‑135 (MQ=255)                                                                                                  ggcCCCCACGCGCTGACGCGCCTGGGTGACAGCGC‑GTGGGCCAGCAGGTTCATCCCCGCTTCGCCATCCGCGCTCAGGTTCAGACGGCGAACAACATCGGTAATTGGCTCATTGCACCACCATCCAGATAACCAt                                <  1:292008/135‑1 (MQ=255)                                                                                                  ggcCCCCACGCGCTGACGCGCCTGGGTGACAGCGC‑GTGGGCCAGCAGGTTCATCCCCGCTTCGCCATCCGCGCTCAGGTTCAGACGGCGAACAACATCGGTAATTGGCTCATTGCACCACCATCCAGATAACCATGTccc                           <  2:1431598/140‑1 (MQ=255)                                                                                                     ccccACGCGCTGACGCGCCTGGGTGACAGCGC‑GTGGGCCAGCAGGTTCATCCCCGCTTCGCCATCCGCGCTCAGGTTCAGACGGCGAACAACATCGGTAATTGGCTCATTGCACCACCATCCAGATAACCATGTCCCCCg                        <  2:145778/140‑1 (MQ=255)                                                                                                                gACGCGCCTGGGTGACAGCGC‑GTGGGCCAGCAGGTTCATCCCCGCTTCGCCATCCGCGCTCAGGTTCAGACGGCGAACAACATCGGTAATTGGCTCATTGCACCACCATCCAGATAACCATGTCCCCCGGTAAACGAcgg             <  2:77194/140‑1 (MQ=255)                                                                                                                 aCGCGCCTGGGTGACAGCGC‑GTGGGCCAGCAGGTTCATCCCCGCTTCGCCATCCGCGCTCAGGTTCAGACGGCGAACAACATCGGTAATTGGCTCATTGCACCACCATCCAGATGACAATGTCCACCGGTAAACGAcggc            >  1:650684/1‑140 (MQ=255)                                                                                                                           gTGACAGCGC‑GTGGGCCAGCAGGTTCATCCCCGCTTCGCCATCCGCGCTCAGGTTCAGACGGCGAACAACATCGGTAATTGGCTCATTGCACCACCATCCAGTTAACCATGTCCGCCAGTAAACGACGGCGGCGAACGGt  >  1:449923/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                 TCAATGGCAAAAAGTTGCAGGCCGTGCCAGTCATCCTTCAGGTAACGTTCCGCGCCCCTGTCTGTGCGGTCTGGCGGAAGAGCCATTCCACTGGGGCGGCCCCCACGCGCTGACGCGCCTGGGTGACAGCGCTGCGGGCCAGCAGGTTCATCCCCGCTTCGCCATCCGCGCTCAGGTTCAGACGGCGAACAACATCGGTAATTGGCTCATTGCACCACCATCCAGATAACCATGTCCCCCGGTAAACGACGGCGGCGAACGGT  >  NC_000913/1294931‑1295193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑