Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,391,095	T→C	A253A (GCA→GCG)	ycjY ←	putative hydrolase YcjY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,391,095	0	T	C	96.5%	188.8 / NA	58	A253A (GCA→GCG)	ycjY	putative hydrolase YcjY
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (47/9);  minor base A (2/0);  total (49/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATACTGAAATTGCTTCAGCCACATAGGCTTTGCCGTCATAAAGATCCATATGGTTTGCGCCTTCAACAATGTGATAGCGTTTATCCTGGCTTGATGCTCGATCGTACAGGTCGTCACTCATCCATTTGCTCCCTGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAGCATCATAGGTAATAATCTGGTTAAGGCTGCGCAAAGTAGCGTAACCCGGTGCTGTTGGATACTGCGCGCGAGGGG  >  NC_000913/1390962‑1391231                                                                                                                                      |                                                                                                                                          aTACTGAAATTGCTTCAGCCACATAGGCTTTGCCGTCATAAAGATCCATATGGTTTGCGCCTTCAACAATGTGATAGCGTTTATCCTGGCTTGATGCTCGATCGTACAGGTCGTCACTCATCCATTTGCTCCCCGCCTgg                                                                                                                                     >  2:813147/1‑140 (MQ=255) aTACTGAAATTGCTTCAGCCACATAGGCTTTGCCGTCATAAAGATCCATATGGTTTGCGCCTTCAACAATGTGATAGCGTTTATCCTGGCTTGATGCTCGATCGTACAGGTCGTCACTCATCCATTTGCTCCCCGCCTgg                                                                                                                                     <  1:1362312/140‑1 (MQ=255)       aaaTTGCTTCAGCCACATAGGCTTTGCCGTCATAAAGATCCATATGGTTTGCGCCTTCAACAATGTGATAGCGTTTATCCTGGCTTGATGCTCGATCGTACAGGTCGTCACTCATCCATTTGCTCCCCGCCTGGC‑TGccc                                                                                                                              >  1:926389/1‑140 (MQ=255)         aTTGCTTCAGCCACATAGGCTTTGCCGTCATAAAGATCCATATGGTTTGCGCCTTCAACAATGTGATAGCGTTTATCCTGGCTTGATGCTCGATCGTACAGGTCGTCACTCATCCATTTGCTCCCCGCCTGGC‑TGCCCGc                                                                                                                            >  1:380947/1‑140 (MQ=255)          ttGCTTCAGCCACATAGGCTTTGCCGTCATAAAGATCCATATGGTTTGCGCCTTCAACAATGTGATAGCGTTTATCCTGGCTTGATGCTCGATCGTACAGGTCGTCACTCATCCATTTGCTCCCAGCCTGGC‑TGCCCGcc                                                                                                                           >  1:666472/1‑140 (MQ=255)                            ttttGCCGTCATAAAGACCCATTTGTTTTGCGCTTTAAAAAATGTGATAGCTTTTACCCTGGCTTGATGCTCGATCGTACAGGTCTCCACTCACCCATTTGCCCCCCCCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGa                                                                                                         <  2:798391/139‑1 (MQ=255)                               tGCCGTCATAAAGATCCATATGGTTTGCGCCTTCAACAATGTGATAGCGTTTATCCTGGCTTGATGCTCGATCGTACAGGTCGTCACTCATCCATTTGCTCCCCGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTc                                                                                                      >  2:1199738/1‑140 (MQ=255)                               tGCCGTCATAAAGATCCATATGGTTTGCGCCTTCAACAATGTGATAGCGTTTATCCTGGCTTGATGCTCGATCGTACAGGTCGTCACTCATCCATTTGCTCCCCGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTc                                                                                                      >  1:962181/1‑140 (MQ=255)                               tGCCGTCATAAAGATCCATATGGTTTGCGCCTTCAACAATGTGATAGCGTTTATCCTGGCTTGATGCTCGATCGTACAGGACGTCACTCATCCATTTGCTCCCCGCCTGGC‑TGCCCCCCACAATCTGCGTGGGCTGAGTc                                                                                                      >  2:778918/1‑140 (MQ=255)                                  cGTCATAAAGATCCATATGGTTTGCGCCTTCAACAATGTGATAGCGTTTATCCTGGCTTGATGCTCGATCGTACAGGTCGCCACTCATCCACTTGCTCCCAGCCAGGC‑AGCCCGCCATAATCCGCGTCGGCTGAGTCAgg                                                                                                   >  2:511020/1‑140 (MQ=255)                                    taaTAAGGCTCCATATGGTTGGCCCTTCCAACACTGAGATGGCGTTTTCCCGGTCTTGGTGCTAGTGCGAACAGGCCCTCCCCCA‑CCAATTTCCCCCCCCCGGGCTTGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTa                                                                                                 <  2:1397897/138‑1 (MQ=255)                                    acaaaaaGATCAATATTTTTCCCGCCTAAAAAATTGTAATGGTTTTTATCCTGGCTTGTTGCCCGTTCGAACGGGTCGTCCCCCATCTTTTTCCCCCCCCCCTGCC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTa                                                                                                 <  2:1058275/136‑1 (MQ=255)                                         aaGATCCATAGGGTTGGCCCTTTCAACAATGTGATGGCTTTTATCCTGGCTGGATCCTGGATCGTCCAGGTCGTCACTCATCTTTTTGCCCCCCCCCTGGC‑TGCCCGCCACAATCTGCGTTGGCTGAGTCAGGTACACtt                                                                                            <  2:808815/140‑1 (MQ=255)                                              ccATATGTTTTGCGCCTCAAAAGATGGAATACCTTGTATTCTGGCTTGCTGCTGGATCGTACGGGTCGCCCCTCACCCTTTTGCCCCCCGCCGGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGcc                                                                                       <  2:628189/140‑1 (MQ=255)                                              ccATATGGTTTGCGCCTTCAACAATGTGATAGCGTTTATCCTGGCTTGATGCTCGATCGTACAGGTCGTCACTCATCCATTTGCTCCCCGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGcc                                                                                       >  1:1397897/1‑140 (MQ=255)                                              ccATATGGTTTCCCCCTTAAACAATGTGATCGCTTTTTTCCTGGCTTGATGCTCGTGCGTACAGGTCTTCACCCATCCTTTTGCCCCCCGCCTGGC‑GGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGcc                                                                                       <  2:211355/140‑1 (MQ=255)                                               cATATGGTTTGCGCCTTCAACAATGTGATAGCGTTTATCCTGGCTTGATGCTCGATCGTACAGGTCGTCACTCATCCATTTGCTCCCCGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCa                                                                                      >  1:1542094/1‑140 (MQ=255)                                                atatGGTTTGCGCCTTCAACAATGTGATAGCGTTTATCCTGGCTTGATGCTCGATCGTACAGGTCGTCACTCATCCATTTGCTCCCCGCCTGGC‑TGCCCGCCGCAATCTGCGTCGGCAGAGTCAAGTACACTTCCGCCat                                                                                     >  1:746314/1‑140 (MQ=255)                                                atatGGTTTGCGCCTTCAACAATGTGATAGCGTTTATCCTGGCTTGATGCTCGATCGTACAGGTCGTCACTCATCCATTTGCTCCCCGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCac                                                                                     >  1:1058275/1‑139 (MQ=255)                                                 tatGGTTTGCGCCTTCAACAATGTGATAGCGTTTATCCTGGCTTGATGCTCGATCGTACAGGTCGTCACTCATCCATTTGCTCCCCGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCata                                                                                    >  2:968832/1‑140 (MQ=255)                                                 tatGGTTTGCGCCTTCAACAATGTGATAGCGTTTATCCTGGCTTGATGCTCGATCGTACAGGTCGTCACTCATCCATTTGCTCCCCGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCata                                                                                    >  1:976525/1‑140 (MQ=255)                                                   tgttttGCCCCTCAAAAAATGTGATGGTTTTTCTCCTGGCTTGATGCTCGATCGTACAGGTCGTCACTCATCTTTTTCCCCCCCGCCTGGC‑GGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATg                                                                                  <  2:926389/137‑1 (MQ=255)                                                   tGGTTTGCCCCTTAAAAAATGTGATAGTTTTTTTCCTGGTTTGATGCTGGGTCGTACAGGTCGTCCCTCTCCCTTTTGCCCCCCGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATg                                                                                  <  2:341318/140‑1 (MQ=255)                                                       ttGCGCCTTAAAAATTGTGATAGCTTTTACCTTGGTTTGTTGCTCGTTCTTAAGGGTCGTCCCCCACCCTTTTCCCCCCCGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTaa                                                                              <  2:1358996/140‑1 (MQ=255)                                                       ttGCGCCTTAAAAAATGTGATGGCTTTTTCCCTGGCTTGATGCTCGTCCGTACGGGTCGTCCCTCACCCTTTTGCCCCCCGCCTGGC‑GGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTaa                                                                              <  2:962181/140‑1 (MQ=255)                                                        tGCGCCTTCAACAATGTGATAGCGTTTATCCTGGCTTGATGCTCGATCGTACAGGTCGTCACTCATCCATTTGCTCCCCGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAg                                                                             >  1:42351/1‑140 (MQ=255)                                                        tGCGCCTTCAACAATGTGATAGCGTTTATCCTGGCTTGATGCTCGATCGTACAGGTCGTCACTCATCCATTTGCTCCCCGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAg                                                                             >  1:1121064/1‑140 (MQ=255)                                                        tGCGCCTTCAACAATGTGATAGCGTTTATCCTGGCTTGATGCTCGATCGTACAGGTCGTCACTCATCCATTTGCTCCCCGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAg                                                                             >  1:810455/1‑140 (MQ=255)                                                        tGCGCCTTCAACAATGTGATAGCGTTTATCCTGGCTTGATGCTCGATCGTACAGGTCGTCACTCATCCATTTGCTCCCCGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAg                                                                             >  1:1469763/1‑140 (MQ=255)                                                        tGCGCCTTCAACAATGTGATAGCGTTTATCCTGGCTTGATGCTCGATCGTACAGGTCGTCACTCATCCATTTGCTCCCCGCCTGGC‑TGCCCACCACAATCAGCGCCGGCTGAGTCAGCTACACTTCCGCCATATGGTAAg                                                                             >  2:656213/1‑140 (MQ=255)                                                        tGCGCCTTCAACAATGTGATAGCGTTTATCCTGGCTTGATGCTCGATCGTACAGGTCGTCACTCATCCATTTGCTCCCCGCCTGGC‑TCCCCGCCACAATCTGGGTCGGCTCAGTCAGCGACACTGCCGCCATATGGTaac                                                                             >  1:539185/1‑139 (MQ=255)                                                            ccTTCAACAATGTGATAGCGTTTATCCTGGCTTGATGCTCGATCGTACAGGTCGTCACTCATCCATTTGCTCCCCGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAGcatc                                                                         >  2:1090218/1‑140 (MQ=255)                                                              ttaaaaaaTTTGATAGCTTTTACCCGGGCTGGATGCTCGATCGTACGGTTCGTCCCTCATCCTTTTCCCCCCCGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAGcatcat                                                                       <  2:1469763/134‑1 (MQ=255)                                                              ttaaaaaaTGTGTTGGCCTTTCTCCTGGTTTTGTCCCCGGTCTTACGGGTCTTCCCTCACCATTTTGCCCCCCGCTTGCC‑GCCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAGcatcat                                                                       <  2:580101/134‑1 (MQ=255)                                                               tCAACAATGTGATAGCGTTTATCCTGGCTTGATGCTCGATCGTACAGGTCGTCACTCATCCATTTGCTCCCCGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAGCATCATa                                                                      >  1:1184239/1‑140 (MQ=255)                                                               tCAACAATGTGATAGCGTTTATCCTGGCTTGATGCTCGATCGTACAGGTCGTCACTCATCCATTTGCTCCCCGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAGCATCATa                                                                      >  2:1441256/1‑140 (MQ=255)                                                                        tgATGGCTTTTACCCTGGCTTGATGCCCGACCGTACAGGTCGTCACTCACCCTTTTCCCCCCCGCCGGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAGCATCATAGGTaataat                                                             <  2:810455/140‑1 (MQ=255)                                                                        tgATAGCTTTTATCCTGGTTGGATGCTCGATCGTACGGGTCGCCACTCATCCTTTTGCCCCCCGCCTGGC‑GGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAGCATCATAGGTaataat                                                             <  2:1542094/140‑1 (MQ=255)                                                                        tgATAGCTTTTATCCTGGCTTGATGCTCGATCGTACAGGTCGTCACTCATCCATTTGCCCCCCGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAGCATCATAGGTaataat                                                             <  1:989488/140‑1 (MQ=255)                                                                         gATAGCGTTTATCCTGGCTTGATGCTCGATCGTACAGGTCGTCACTCATCCATTTGCTCCCCGCCTGGC‑TGCCCGCCACAATCTGCGTCGGGTGAGTCAGGTACACTTGCGCCATATGGTAAACATCATAGGTAATAATc                                                            >  2:482996/1‑140 (MQ=255)                                                                           tAGCGTTTATCCTGGCTTGATGCTCGATCGTACAGGTCGTCACTCATCCATTTGCTCCCCGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAGCATCATAGGtaatctgtct                                                          >  1:1013564/1‑134 (MQ=255)                                                                           tAGCGTTTATCCTGGCTTGATGCTCGATCGTACAGGTCGTCACTCATCCATTTGCTCCCCGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAGCATCATAGGTAATAATCTg                                                          >  2:916626/1‑140 (MQ=255)                                                                           tAGCGTTTATCCTGGCTTGATGCTCGATCGTACAGGTCGTCACTCATCCATTTGCTCCCCGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAGCATCATAGGTAATAATCTg                                                          >  2:1359941/1‑140 (MQ=255)                                                                                tttATCCTGGCTTGATGCTCGATCGTACAGGTCGTCACTCATCCATTTGCTCCCCGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCGGGTACACTGCCGGCATGTGGTAAGCGTCATGGGTAATAATCTGGTTaa                                                     >  2:405727/1‑140 (MQ=255)                                                                                  tATCCTGGCTTGATGCTCGATCGTACAGGTCGTCACTCATCCATTTGCTCCCCGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAGCATCATAGGTAATAATCTGGTTAAgg                                                   >  1:944893/1‑140 (MQ=255)                                                                                  tATCCTGGCTTGATGCTCGATCGTACAGGTCGTCACTCATCCATTTGCTCCCCGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGACATATGGAAAGGATAATAGGTAATAATCTGGTTAAgg                                                   >  1:495565/1‑140 (MQ=255)                                                                                       gggtttGACGCTGGATCGTCCGGGCCGTCCCTCCTCCTTCTGCCCCCCTCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAGCATCATAGGTAATAATCTGGTTAAGGCTgcg                                              <  2:731998/136‑1 (MQ=255)                                                                                        ggCTTGATGCTCGATCGTACAGGTCGTCACTCATCCATTTGCTCCCCGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTTAGTCAGGTACACTTCCGCCATATGGTAAGCATCATAGGTAATAATCTGGTTAAGGCTgcgc                                             >  1:1471496/1‑140 (MQ=255)                                                                                        ggCTTGATGCTCGATCGTACAGGTCGTCACTCATCCATTTGCTCCCCGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAGCATCATAGGTAATAATCTGGTTAAGGCTgcgc                                             >  2:1301172/1‑140 (MQ=255)                                                                                              aTGCTCGATCGTACAGGTCGTCACTCATCCATTTGCTCCCCGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAGCATCATAGGTAATAATCTGGTTAAGGCTGCGCAAAGTa                                       >  1:109844/1‑140 (MQ=255)                                                                                              aTGCTCGATCGTACAGGTCGTCACTCATCCATTTGCTCCCCGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAGCATCATAGGTAATAATCTGGTTAAGGCTGCGCAAAGTa                                       >  2:136023/1‑140 (MQ=255)                                                                                              aTGCTCGATCGTACAGGTCGTCACTCATCCATTTGCTCCCCGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCAATATGGTAGTCAGCATGGGTAATAACCTGGTTAAGGCTGCGCAAAGTa                                       >  2:654094/1‑140 (MQ=255)                                                                                              aTGCTCGATCGTACAGGCCGTCACTCATCCATTTGCTCCCCGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAGAATCATAGGTAAGAAGCTGGTTGAGGCTGCGCAAAGTa                                       >  1:448132/1‑140 (MQ=255)                                                                                               gagacagtCGTAAGGGTCGTCACCAACCTTTTTCCCCCCCGCCGGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAGCATCATAGGTAATAATCTGGTTAAGGCTGCGCAAAGTAg                                      <  2:326612/133‑1 (MQ=255)                                                                                                         tACAGGTCGTCACTCATCCATTTGCTCCCCGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAGCATCATAGGTAATAATCTGGTTAAGGCTGCGCAAAGTAGCGTAACCCgg                            <  1:1199738/140‑1 (MQ=255)                                                                                                         tACAGGTCGTCACTCATCCATTTGCTCCCCGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAGCATCATAGGTAATAATCTGGTTAAGGCTGCGCAAAGTAGCGTAACCCgg                            >  1:230449/1‑140 (MQ=255)                                                                                                         tACAGGTCGTCACTCATCCATTTGCTCCCCGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAGCATCATAGGAAATAATCTGGTTAAGGCTGCGCAAAGTAGCGTAACCCgg                            >  1:1138096/1‑140 (MQ=255)                                                                                                           cAGGTCGTCACTCATCCATTTGCTCCCCGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAGCATCATAGGTAATAATCTGGTTAAGGCTGCGCAAAGTAGCGTAACCCGGTg                          <  1:1441256/140‑1 (MQ=255)                                                                                                           cAGGTCGTCACTCATCCATTTGCTCCCCGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAGCAGCATAGGTAGTAATCTGGATAAGGCTTCGCACTGTAGCGAGACCCGGTg                          >  2:741710/1‑140 (MQ=255)                                                                                                            aGGTCGTCACTCATCCATTTGCTCCCCGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAGCATCATAGGTAATAATCTGGTTAAGGCTGCGCAAAGTAGCGTAACCCGGTGc                         >  1:2757/1‑140 (MQ=255)                                                                                                              gtcgtcACTCATCCATTTGCTCCCCGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAGCATCATAGGTAATAATCTGGTTAAGGCTGCGCAAAGTAGCGTAACCCGGTGCTg                       <  1:813147/140‑1 (MQ=255)                                                                                                              gtcgtcACTCATCCATTTGCTCCCCGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAGCATCATAGGTAATAATCTGGTTAAGGCTGCGCAAAGTAGCGTAACCCGGTGCTg                       >  1:156253/1‑140 (MQ=255)                                                                                                              gcctccccTCACCCATTTCCCCCCCCCCTGCC‑TCCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAGCATCATAGGTAATAATCTGGTTAAGGCTGCGCAAAGTAGCGTAACCCGGTGCTg                       <  2:230449/133‑1 (MQ=255)                                                                                                                cgtcTCTCATCCATTTGCTCCCCGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAGCATCATAGGTAATAATCTGGTTAAGGCTGCGCAAAGTAGCGTAACCCGGTGCTGtt                     >  1:1172008/1‑140 (MQ=255)                                                                                                                cgtcACTCATCCATTTGCTCCCCGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAGCATCATAGGTAATAATGTGGCTAACGCTGCGCAGAGTACCGTAACCCGGTGCTGtt                     >  2:649500/1‑140 (MQ=255)                                                                                                                cgtcACTCATCCATTTGCTCCCCGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAGCATCATAGGTAATAATCTGGTTAAGGGTGCACAGAGTAGCGTAACCCGGTGCTGtt                     >  1:455554/1‑140 (MQ=255)                                                                                                                cgtcACTCATCCATTTGCTCCCCGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAGCATCATAGGTAATAATCTGGTTAAGGCTGCGCAAAGTAGCGTAACCCGGTGCTGtt                     >  2:1312507/1‑140 (MQ=255)                                                                                                                cgtcACTCATCCATTTGCTCCCCGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAGCATCATAGGTAATAATCTGGTTAAGGCTGCGCAAAGTAGCGTAACCCGGTGCTGtt                     >  1:1533395/1‑140 (MQ=255)                                                                                                                cgtcACTCATCCATTTGCTCCCCGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAGCATCATAGGTAATAATCTGGTTAAGGCTGCGCAAAGTAGCGTAACCCGGTGCTGtt                     >  2:772721/1‑140 (MQ=255)                                                                                                                cgtcACTCATCCATTTGCTCCCCGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAGCATCATAGGTAATAATCTAGTTAAGGATGCGCAAAGTAGCGTAACCCGGTGCTGtt                     >  1:441756/1‑140 (MQ=255)                                                                                                                 gtcACTCATCCATTTGCTCCCCGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAGCATCATAGGTAATAATCTGGTTAAGGCTGCGCAAAGTAGCGTAACCCGGtctgtctc                    >  1:326612/1‑133 (MQ=255)                                                                                                                   cccTCACCCTTTTCCCCCCCGCCGGGC‑TCCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAGCATCATAGGTAATAATCTGGTTAAGGCTGCGCAAAGTAGCGTAACCCGGTGCTGTTGGa                  <  2:944893/138‑1 (MQ=255)                                                                                                                        aTCCATTTGCTCCCCGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAGCATCATAGGTAATAATCTGGTTAAGGCTGCGCAAAGTAGCGTAACCCGGTGCTGTTGGATACTg             <  1:916626/140‑1 (MQ=255)                                                                                                                        aTCCATTTGCTCCCCGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAGCATCATAGGTAATAATCTGGTTAAGGCTGCGCAAAGTAGCGTAACCCGGTGCTGTTGGATACTg             >  2:253918/1‑140 (MQ=255)                                                                                                                              ttGCTCCCCGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAGCATCATAGGTAATAATCTGGTTAAGGCTGCGCAAAGTAGCGTAACCCGGTGCTGTTGGATACTGcgcgcg       <  1:253918/140‑1 (MQ=255)                                                                                                                              ttGCCCCCCGCTTGGC‑TCCCCGACACAATCTCCGTAGGCTGAGTCAGGAACACTTCCGCCATATGGTAAGCATCATAGGTAATAATCTGGTTAAGGCTGCGCAAAGTAGCGTAACCCGGTGCTGTTGGATACTGcgcgcg       <  2:608365/140‑1 (MQ=255)                                                                                                                                gCTCCCCGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAGCATCATAGGTAATAATCTGGTTAAGGCTGCGCAAAGTAGCGTAACCCGGTGCTGTTGGATACTGCGCGCGAg     <  1:772721/140‑1 (MQ=255)                                                                                                                                   ccccGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAGCATCATAGGTAATAATCTGGTTAAGGCTGCGCAAAGTAGCGTAACCCGGTGCTGTTGGATACTGCGCGCGAgggg  <  1:1090218/140‑1 (MQ=255)                                                                                                                                   ccccGCCTGCC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAGCATCATAGGTAATAATCTGGTTAAGGCTGCGCAAAGTAGCGTAACCCGGTGCTGTTGGATACTGCGCGCGAgggg  <  2:1175620/140‑1 (MQ=255)                                                                                                                                   ccccGCCGGGC‑CCCCCGCCACAATCTGCGTCCGCTCAGTGAGGTACACTTCCGCCATATGGTAAGCATCATAGGTAATAATCTGGTTAAGGCTGCGCAAAGTAGCGTAACCCGGTGCTGTTGGATACTGCGCGCGAgggg  <  2:547853/140‑1 (MQ=255)                                                                                                                                   ccccGCCAGGA‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAGCATCATAGGTAATAATCTGGTTAAGGCTGCGCAAAGTAGCGTAACCCGGTGCTGTTGGATACTGCGCGCGAgggg  <  1:778918/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                          ATACTGAAATTGCTTCAGCCACATAGGCTTTGCCGTCATAAAGATCCATATGGTTTGCGCCTTCAACAATGTGATAGCGTTTATCCTGGCTTGATGCTCGATCGTACAGGTCGTCACTCATCCATTTGCTCCCTGCCTGGC‑TGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAGCATCATAGGTAATAATCTGGTTAAGGCTGCGCAAAGTAGCGTAACCCGGTGCTGTTGGATACTGCGCGCGAGGGG  >  NC_000913/1390962‑1391231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑