Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,535,998	A→G	V207A (GTG→GCG)	narV ←	nitrate reductase Z subunit gamma

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,535,998	0	A	G	100.0%	231.2 / NA	67	V207A (GTG→GCG)	narV	nitrate reductase Z subunit gamma
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (44/23);  total (44/23)

CTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACACATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACGAACGCTACGCCGTTGAGCATCTCTGACGATCCACCACGGAAAGTGACAATGCTTTGCGCCCAA  >  NC_000913/1535866‑1536132                                                                                                                                     |                                                                                                                                       ctcCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACGCATGCAc                                                                                                                                 >  2:1496121/1‑140 (MQ=255) ctcCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACGCATGCAc                                                                                                                                 <  1:236862/140‑1 (MQ=255)    cTGTGACTGGAGAACTATCATAGCCTCCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACGCATGCACCAg                                                                                                                              >  2:1234505/1‑140 (MQ=255)           tGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGTCGAGTAAAGTACCCAAACGGCGCGCGCCACACATGCACCAGTCGGGTg                                                                                                                       >  2:642218/1‑140 (MQ=255)            ggAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCTCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTGa                                                                                                                      <  2:1459857/140‑1 (MQ=255)             gagacagaTCATAGCCGGCAAGAGGCCGGAGAGCGCAGGACTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTGaa                                                                                                                     <  2:687770/133‑1 (MQ=255)              agaACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTGAAc                                                                                                                    >  2:1494498/1‑140 (MQ=255)                 aCTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTGAACggg                                                                                                                 <  2:1025165/140‑1 (MQ=255)                   tATCATAGCCTGCAAGTTGCCGCAGAGCGAAGGGCCATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTGAACGGGaa                                                                                                               <  2:683185/140‑1 (MQ=255)                     tCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCCGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGAGCGCTCCTCGCGTGTACCAGTAGGGTGAACGGGAaga                                                                                                             >  1:732469/1‑140 (MQ=255)                       aTAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTg                                                                                                                       <  2:834659/128‑1 (MQ=255)                       aTAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTg                                                                                                                       >  1:834659/1‑128 (MQ=255)                        tAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCa                                                                                                          >  1:375045/1‑140 (MQ=255)                        tAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCa                                                                                                          >  1:1518655/1‑140 (MQ=255)                            cTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACGCATGTACCAGTCGGGTGAACGGGAAGAGCAGGaa                                                                                                      >  2:942445/1‑140 (MQ=255)                             tGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAg                                                                                                     >  2:876540/1‑140 (MQ=255)                             tGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCACTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAg                                                                                                     >  1:797242/1‑140 (MQ=255)                              gCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGa                                                                                                    <  2:52827/140‑1 (MQ=255)                               cAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACGCATGCACTAGTAGGGTGAACGCGAAGAGCactgtctc                                                                                                   >  1:687770/1‑133 (MQ=255)                               cAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACGCATGCACGAGTTGGGTGAACGGGAAGAGCAGGAAGAt                                                                                                   >  1:614874/1‑140 (MQ=255)                               cAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGa                                                                                                    >  2:1450172/1‑139 (MQ=255)                               cAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGa                                                                                                    <  1:1450172/139‑1 (MQ=255)                               cAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGAt                                                                                                   >  1:331641/1‑140 (MQ=255)                               cAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGAt                                                                                                   >  2:251921/1‑140 (MQ=255)                               cAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGAt                                                                                                   >  1:1544680/1‑140 (MQ=255)                               cAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGAt                                                                                                   >  2:1391976/1‑140 (MQ=255)                               cAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGAt                                                                                                   >  1:1488412/1‑140 (MQ=255)                               cAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGAt                                                                                                   >  1:332621/1‑140 (MQ=255)                                 aGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATgg                                                                                                 >  1:849479/1‑140 (MQ=255)                                   tGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTc                                                                                               <  2:1544680/140‑1 (MQ=255)                                    ggCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCa                                                                                              <  1:1496121/140‑1 (MQ=255)                                    ggCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCa                                                                                              <  2:1518655/140‑1 (MQ=255)                                         gagaGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTccc                                                                                         <  2:332621/140‑1 (MQ=255)                                          agagCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCa                                                                                        <  2:331641/140‑1 (MQ=255)                                          agagCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCACTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCa                                                                                        <  2:797242/140‑1 (MQ=255)                                                        tCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAgg                                                                          <  2:82290/140‑1 (MQ=255)                                                                      aaTTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACGAACGc                                                            >  2:1147404/1‑140 (MQ=255)                                                                      aaTTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGCTCATTCCAAACGCCAGATGCAAGCGGAAGACGAACGc                                                            >  1:749569/1‑140 (MQ=255)                                                                       aTTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACGAACGCt                                                           >  2:1213267/1‑140 (MQ=255)                                                                           tCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACGAACGCTACGc                                                       >  1:925933/1‑140 (MQ=255)                                                                           tCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACGAACGCTACGc                                                       >  1:1026696/1‑140 (MQ=255)                                                                            cgccgcGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACGAACGCTACGcc                                                      >  2:120102/1‑140 (MQ=255)                                                                            cgccgcGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACGAACGCTACGcc                                                      >  1:1174845/1‑140 (MQ=255)                                                                               cgcgAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGA‑GGTCATTCCCAACACCAGATGCAGGCGGAAGACGAACGCTACGCCGTTg                                                  <  1:876540/140‑1 (MQ=255)                                                                               cgcgAACGCACAATTTGATATCGACGAG‑AAAGTACTCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACGAACGCTACGCCGTTg                                                  >  1:287978/1‑140 (MQ=255)                                                                                  gAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCGCGCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACGAACGCTACGCCGTTGAg                                                <  1:1147404/140‑1 (MQ=255)                                                                                   aaCGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACGAACGCTACGCCGTTGAGc                                               >  1:768291/1‑140 (MQ=255)                                                                                    aCGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACGAACGCTACGCCGTTGAGCa                                              <  1:917844/140‑1 (MQ=255)                                                                                        acaATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACGAACGCTACGCCGTTGAGCAtctc                                          <  2:768291/140‑1 (MQ=255)                                                                                           aTTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACGAACGCTACGCCGTTGAGCATCTCTGa                                       >  1:1450702/1‑140 (MQ=255)                                                                                           aTTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACGAACGCTACGCCGTTGAGCATCTCTGa                                       <  2:849479/140‑1 (MQ=255)                                                                                              tGATATCGACGAG‑AAAGTACTCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACGAACGCTACGCCGTTGAGCATCTCTGACGAt                                   <  2:287978/140‑1 (MQ=255)                                                                                                      acgaGTAAAGTACTCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCCGGCGGAAGCCGAACGCCACGCCGTTAAGCACATCTGACGCTCcaccac                            >  2:742803/1‑140 (MQ=255)                                                                                                      acgaGTAAAGTACTCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACGAACGCTACGCCGTTGAGCATCTCTGACGATCcaccac                            >  1:766031/1‑140 (MQ=255)                                                                                                      acgaGTAAAGTACTCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACGAACGCTACGCCGTTGAGCATCTCTGACGATCcaccac                            >  2:838223/1‑140 (MQ=255)                                                                                                      acgaGTAAAGTACTCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACGAACGCTACGCCGTTGAGCATCTCTGACGATCcaccac                            >  1:928667/1‑140 (MQ=255)                                                                                                      acgaGTAAAGTACTCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACGAACGCCCCGCCGTTGAGCAAGTCTGACGATCcaccac                            >  2:740221/1‑140 (MQ=255)                                                                                                      acgaGTAAAGTACTCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACAAACGCTACGCCGTTTAGCATCCCTGACGATCcaccac                            >  1:681202/1‑140 (MQ=255)                                                                                                      acgaGTAAAGTACTCAAACGGCGCGCTCCACGAATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACTAACGCTAGGCCTTTAAGCATCGCTGACGATCcaccac                            >  1:458989/1‑140 (MQ=255)                                                                                                          gTAAAGTACTCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACGAACGCTACGCCGTTGAGCATCTCTGACGATCCACCACGGaa                        >  2:280131/1‑140 (MQ=255)                                                                                                          gTAAAGTACTCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACGAACGCTACGCCGTTGAACATCTCTGACGATCCACCACGGaa                        <  1:251921/140‑1 (MQ=255)                                                                                                           tAAAGTACTCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTGAACGGGAATAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACGAACGCTACGCCGTTGAGCATCTCTGACGATCCACCACGGaaa                       >  1:182627/1‑140 (MQ=255)                                                                                                                  cTCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACGAACGCTACGCCGTTGAGCATCTCTGACGATCCACCACGGAAAGTGACaa                <  1:1391976/140‑1 (MQ=255)                                                                                                                   tCAAACGGCGCGCTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACGAACGCTACGCCGTTGAGCATCTCTGACGATCCACCACGGAAAGTGACAAt               >  1:94893/1‑140 (MQ=255)                                                                                                                    cAAACGGCGCGCTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACGAACGCTACGCCGTTGGGCAGCTCTGACGCTCCACCACGGAAAGTGACAATg              >  2:605239/1‑140 (MQ=255)                                                                                                                     aaaCGGCGCGCTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACGAACGCTACGCCGTTGAGCATCTCTGACGATCCACCACGGAAAGTGACAATGc             >  1:272043/1‑140 (MQ=255)                                                                                                                     aaaCGGCGCGCTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACGAACGCTACGCCGATGAGCATATCTGACGAGCCACCACGGAAAGTGACAATGc             >  2:504242/1‑140 (MQ=255)                                                                                                                          gcgcgcTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACGAACGCTACGCCGTTGAGCATCTCTGACGATCCACCACGGAAAGTGACAATGCTTTgc        >  1:215999/1‑140 (MQ=255)                                                                                                                          gcgcgcTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACGAACGCTACGCCGTTGAGCATCTCTGACGATCCACCACGGAAAGTGACAATGCTTTgc        >  1:932750/1‑140 (MQ=255)                                                                                                                             cgcTCCACGCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACGAACGCTACGCCGTTGAGCATCTCTGACGATCCACCACGGAAAGTGACAATGCTTTGCGcc     >  2:1409897/1‑140 (MQ=255)                                                                                                                                tCCACGCATGCACCAGTGGGGTCAACGGGAAGAGCAGGAAGATGGTCAGTCCCAACACCAGATGCAGGCGGAAGACGAACGCTACGCCGTTGAGCATCTCTGACGATCCACCACGGAAAGTGACAATGCTTTGCGCCCaa  <  2:681202/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                       CTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACACATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACGAACGCTACGCCGTTGAGCATCTCTGACGATCCACCACGGAAAGTGACAATGCTTTGCGCCCAA  >  NC_000913/1535866‑1536132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑