Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,569,819	A→G	V224A (GTC→GCC)	gadC ←	L‑glutamate:4‑aminobutyrate antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,569,819	0	A	G	100.0%	331.4 / NA	91	V224A (GTC→GCC)	gadC	L‑glutamate:4‑aminobutyrate antiporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (45/46);  total (45/46)

GCGGAGAGGTTGATTTCATTACCCGGAATGACCATCGCAATAGACAAACCACCAACAGAGCTTAAGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGACGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTACCAGGGTGCCCACTTTAGAGAAGTCAGGGAAGAAGGTCTTCGAATCCATTTCGATAGCAACGGGGGCACCGGAGTGC  >  NC_000913/1569684‑1569953                                                                                                                                        |                                                                                                                                       gCGGAGAGGTTGATTTCATTACCCGGAATGACCATCGCAATAGACAAACCACCAACAGAGCTTAAGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTg                                                                                                                                    >  2:161179/1‑140 (MQ=255)       agGTTGATTTCATTACCCGGAATGGCCATCGCAATAGACAAACCACCAACAGAGCTTAAGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGc                                                                                                                              >  2:1024838/1‑140 (MQ=255)       agGTTGATTTCATTACCCGGAATGACCATCGCAATAGACAAACCACCAACAGAGCTTAAGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGc                                                                                                                              >  2:189927/1‑140 (MQ=255)         gTTGATTTCATTACCCGGAATGACCATCGCAATAGACAAACCACCAACAGAGCTTAAGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCgg                                                                                                                            >  2:944451/1‑140 (MQ=255)         gTTGATTTCATTACCCGGAATGACCATCGCAATAGACAAACCACCAACAGAGCTTAAGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCgg                                                                                                                            >  1:952190/1‑140 (MQ=255)          ttGATTTCATTACCCGGAATGACCATCGCAATAGACAAACCACCAACAGAGCTTAAGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGa                                                                                                                           >  1:936780/1‑140 (MQ=255)          ttGATTTCATTACCCGGAATGACCATCGCAATAGACAAACCACCAACAGAGCTTAAGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGa                                                                                                                           <  1:1240845/140‑1 (MQ=255)             aTTTCATTACCCGGAATCACCAGCGCAATAGTCAACCCATCAACAGAGCTTAAGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGc                                                                                                                        <  2:482657/140‑1 (MQ=255)                 cATTACCCGGAATGACCATCGCAATAGACAAACCACCAACAGAGCTTAAGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCt                                                                                                                    >  1:1417882/1‑140 (MQ=255)                     aCCCGGAATGACCATCGCAATAGACAAACCACCAACAGAGCTTAAGCAGATTGCCGCTACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGc                                                                                                                <  2:1517318/140‑1 (MQ=255)                     aCCCGGAATGACCATCGCAATAGACAAACCACCAACAGAGCTTAAGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGc                                                                                                                <  2:1482135/140‑1 (MQ=255)                         ggAATGACCATCGCAATCGACATACCACCAACAGACCTTCAGCAGATTTCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCat                                                                                                            <  2:416741/140‑1 (MQ=255)                         ggAATGACCATCGCAATAGACAAACCACCAACAGAGCTTAAGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCat                                                                                                            >  2:1087327/1‑140 (MQ=255)                           aaTGACCATCGCAATAGACAAACCACCAACAGAGCTTAAGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCatat                                                                                                          >  1:1395822/1‑140 (MQ=255)                                ccATCGCAATAGACAAACCACCAACAGAGCTTAAGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTc                                                                                                     >  2:92486/1‑140 (MQ=255)                                   tCGCAATAGACAAACCACCAACAGAGCTTAAGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCaaa                                                                                                  >  1:1164705/1‑140 (MQ=255)                                      cAATAGACAAACCACCAACAGAGCTTAAGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATg                                                                                               <  2:1164705/140‑1 (MQ=255)                                         tAGACAAACCACCAACATAGCTTAAGCAGATTGCCGCCACCATCAGTAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAg                                                                                            <  2:601166/140‑1 (MQ=255)                                         tAGACAAACCACCAACAGAGCTTAAGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAg                                                                                            >  1:1369577/1‑140 (MQ=255)                                         tAGACAAACCACCAACAGAGCTTAAGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAg                                                                                            <  2:1417882/140‑1 (MQ=255)                                         tAGACAAACCACCAACAGAGCTTAAGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTATACGCCCATCTAACTCAAAATGAAg                                                                                            >  1:585995/1‑140 (MQ=255)                                              aaaCCACCAACAGAGCTTAAGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGcaac                                                                                       >  1:1218947/1‑140 (MQ=255)                                              aaaCCACCAACAGAGCTTAAGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGcaa                                                                                        >  2:1023348/1‑139 (MQ=255)                                              aaaCCACCAACAGAGCTTAAGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGcaa                                                                                        <  1:1023348/139‑1 (MQ=255)                                                 ccaccaACAGAGCTTTAGCAGATTGCCGCTACCATCAACAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACaaa                                                                                    <  1:666363/140‑1 (MQ=255)                                                 ccaccaACAGAGCTTGAGCAGAGTGCCGCGACCATCAACAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACaaa                                                                                    <  1:561102/140‑1 (MQ=255)                                                 ccaccaACAGAGCTTAAGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACaaa                                                                                    <  1:161179/140‑1 (MQ=255)                                                     caACAGAGCTTAAGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACaaa                                                                                    >  2:1059555/1‑136 (MQ=255)                                                     caACAGAGCTTAAGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACaaa                                                                                    <  1:1059555/136‑1 (MQ=255)                                                     caACAGAGCTTAAGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAAtact                                                                                >  1:101692/1‑140 (MQ=255)                                                     caACAGAGCTTAAGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAAtact                                                                                >  2:316828/1‑140 (MQ=255)                                                     caACAGAGCTTAAGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAAtact                                                                                >  1:1507506/1‑140 (MQ=255)                                                     caACAGAGCTTAAGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAAtact                                                                                >  1:1197841/1‑140 (MQ=255)                                                     caACAGAGCTTAAGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTAGCGGATGCGTCTCCGCACATATAACGCAAAGTGAAGGCAACAAAtact                                                                                >  2:561102/1‑140 (MQ=255)                                                      aaCAGAGCTTAAGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAAtacta                                                                               >  1:867005/1‑140 (MQ=255)                                                        cAGAGCTTAAGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTAcc                                                                             <  1:1087327/140‑1 (MQ=255)                                                        cAGAGCTTAAGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTAcc                                                                             >  2:1046453/1‑140 (MQ=255)                                                         agagCTTAAGCAGATTGCCGCCACCATCAACAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTACCa                                                                            <  1:1241750/140‑1 (MQ=255)                                                            gCTTAAGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTACCAggg                                                                         >  2:220431/1‑140 (MQ=255)                                                            gCTTAAGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTACCAggg                                                                         >  1:1183601/1‑140 (MQ=255)                                                            gCTTAAGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTACCAggg                                                                         >  2:810687/1‑140 (MQ=255)                                                            gCTTAAGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATACTTCTACGGCCATATAGCTCAAAATGAAGGGAACAAATACTACCAggg                                                                         >  2:416068/1‑140 (MQ=255)                                                               tAAGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTACCAGGGTGc                                                                      <  1:92486/140‑1 (MQ=255)                                                               tAAGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTACCAGGGTGc                                                                      <  2:936780/140‑1 (MQ=255)                                                               tAAGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTACCAGGGTGc                                                                      <  2:1369577/140‑1 (MQ=255)                                                               tAAGCAGATTGCCGCCAACATCTGCAGTAACTTAGTCAAAGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTACCAGGGTGc                                                                      <  2:585995/140‑1 (MQ=255)                                                                aaGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTGTACGCCCACATAACTCCAAATGAAGGCAACAAATACTACCAGGGTGcc                                                                     >  2:577006/1‑140 (MQ=255)                                                                aaGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTACCAGGGTGcc                                                                     <  2:867005/140‑1 (MQ=255)                                                                aaGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTACCAGGGTGcc                                                                     <  2:1395822/140‑1 (MQ=255)                                                                aaGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTACCAGGGTGcc                                                                     <  2:952190/140‑1 (MQ=255)                                                                aaGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTACCAGGGTGcc                                                                     <  2:1341947/140‑1 (MQ=255)                                                                aaGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTACCAGGGTGcc                                                                     <  2:1273408/140‑1 (MQ=255)                                                                aaGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTACCAGGGTGcc                                                                     <  2:1218947/140‑1 (MQ=255)                                                                aaGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTACCAGGGTGcc                                                                     <  1:189927/140‑1 (MQ=255)                                                                aaGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTACCAGGGTGcc                                                                     <  1:789871/140‑1 (MQ=255)                                                                aaGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTACCAGGGTGcc                                                                     <  1:944451/140‑1 (MQ=255)                                                                aaGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTACCAGGGTGcc                                                                     <  1:1042327/140‑1 (MQ=255)                                                                aaGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTACCAGGGTGcc                                                                     <  2:101692/140‑1 (MQ=255)                                                                  gCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTACCAGGGTGCCCa                                                                   <  2:905039/140‑1 (MQ=255)                                                                  gCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTACCAGGGTGCCCa                                                                   >  1:148970/1‑140 (MQ=255)                                                                     gATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTACCAGGGTGc                                                                      <  2:1294646/134‑1 (MQ=255)                                                                     gATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTACCAGGGTGc                                                                      >  1:1294646/1‑134 (MQ=255)                                                                         gccgccACCATCAGCTGTAACACAGTCAACGGATAGTTGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTACCAGGGTGCCCACTTTaga                                                            <  1:718339/140‑1 (MQ=255)                                                                            gccACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTACCAGGGTGCCCACTTTAGAGaa                                                         <  1:316828/140‑1 (MQ=255)                                                                                           aaCATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTACCAGGGTGCCCACTTTaga                                                            >  2:1031614/1‑122 (MQ=255)                                                                                           aaCATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTACCAGGGTGCCCACTTTaga                                                            <  1:1031614/122‑1 (MQ=255)                                                                                               tAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTACCAGGGTGCCCACTTTAGAGAAGTCAGGGAAGAAGGTCTTc                                      <  2:1183601/140‑1 (MQ=255)                                                                                                 gCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTACCAGGGTGCCCACTTTAGAGAAGTCAGGGAAGAAGGTCTTCGa                                    >  1:1263291/1‑140 (MQ=255)                                                                                                  ccAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTACCAGGGTGCCCACTTTAGAGAAGTCAGGGAAGAAGGTCTTCg                                     >  2:168754/1‑138 (MQ=255)                                                                                                  ccAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTACCAGGGTGCCCACTTTAGAGAAGTCAGGGAAGAAGGTCTTCg                                     <  1:168754/138‑1 (MQ=255)                                                                                                  ccAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTACCAGGGTGCCCACTTTAGAGAAGTCAGGGAAGAAGGTCTTCGaa                                   >  1:881587/1‑140 (MQ=255)                                                                                                    aaCGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTACCAGGGTGCCCACTTTAGAGAAGTCAGGGAAGAAGGTCTTCGAATc                                 >  1:779966/1‑140 (MQ=255)                                                                                                        gATAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTACAAGGGTGCCAACTATAGAGAAGTCAGGGAAGAAGGTCTTCGAATCCAtt                             >  2:545241/1‑140 (MQ=255)                                                                                                          tAGTCGCGCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTACCAGGGTGCCCACTTTAGAGAAGTCAGGGAAGAAGGTCTTCGAATCCATTTc                           >  1:1020734/1‑140 (MQ=255)                                                                                                              cgcgCCCTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTACCAGGGTGCCCACTTTAGAGAAGTCAGGGAAGAAGGTCTTCGAATCCATTTCGATa                       <  2:779966/140‑1 (MQ=255)                                                                                                                  cccTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTACCAGGGTGCCCACTTTAGAGAAGTCAGGGAAGAAGGTCTTCGAATCCATTTCGATAGCaa                   >  1:54737/1‑140 (MQ=255)                                                                                                                   ccTGGGTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTACCAGGGTGCCCACTTTAGAGAAGTCAGGGAAGAAGGTCTTCGAATCCATTTCGATAGCAAc                  <  2:1263291/140‑1 (MQ=255)                                                                                                                       ggTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTACCAGGGTGCCCACTTTAGAGAAGTCGGGGTAGAGGGTCTTCGAATCCCTTTCGATAGCAACgggg              >  2:462321/1‑140 (MQ=255)                                                                                                                       ggTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTACCAGGGTGCCCACTTTAGAGAAGTCAGTGAAGAAGGTCTTCGGATCCATGTCGATAGCAACgggg              >  1:452442/1‑140 (MQ=255)                                                                                                                        gTTGCTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTACCAGGGTGCCCACTTTAGAGAAGTCAGGGAAGAAGGTCTTCGAATCCATTTCGATAGCAACggggg             <  2:881587/140‑1 (MQ=255)                                                                                                                            cTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTACCAGGGTGCCCACTTTAGAGAAGTCAGGGAAGAAGGTCTTCGAATCCATTTCGATAGCAACGGGGGCAcc         >  2:75908/1‑140 (MQ=255)                                                                                                                            cTCATTTCATTGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTACCAGGGTGCCCACTTTAGAGAAGTCAGGGAAGAAGGTCTTCGAATCCATTTCGATAGCAACGGGGGCAcc         >  2:1418951/1‑140 (MQ=255)                                                                                                                             tcatttcattGGCGTTGGTTGCGGAGGCTTCTACGCCTATATAACTCAAAATGAAGGCAACAAATACTACCAGGGTGCCCACTTTAGAGAAGTCAGGGAAGAAGGTCTTCGAATCCATTTCGATAGCAACGGGGGCACCg        <  1:500767/140‑1 (MQ=255)                                                                                                                             tcatttcattGGCGTGGCTTGCCGATGCTTCGACGCCCATATAACTCATAATGAAGGCAACAAATACTACCAGGGTGCCCACTTTAGAGAAGTCAGGGAAGAAGGTCTTCGAATCCATTTCGATAGCAACGGGGGCACCg        <  2:452442/140‑1 (MQ=255)                                                                                                                                tttcattGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTACCAGGGTGCCCACTTTAGAGAAGTCAGGGAAGAAGGTCTTCGAATCCATTTCGATAGCAACGGGGGCACCGGAg     <  2:1197841/140‑1 (MQ=255)                                                                                                                                 ttcattGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTACCAGGGTGCCCACTTTAGAGAAGTCAGGGAAGAAGGTCTTCGAATCCATTTCGATAGCAACGGGGGCACCgg       <  2:551285/137‑1 (MQ=255)                                                                                                                                 ttcattGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTACCAGGGTGCCCACTTTAGAGAAGTCAGGGAAGAAGGTCTTCGAATCCATTTCGATAGCAACGGGGGCACCgg       >  1:551285/1‑137 (MQ=255)                                                                                                                                 ttcattGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTACCAGGGTGCCCACTTTAGAGAAGTCAGGGAAGAAGGTATTCGAATGCATGTCTATAGCATCGGGGGCACCGGAGt    >  1:424779/1‑140 (MQ=255)                                                                                                                                 ttcattGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTACCAGGGTGCCCACTTTAGAGAAGTCAAGGAAGAAGGTCTCCGAGTCCATTTCGATAGGAACGGGGGCACCGGAGt    >  2:500767/1‑140 (MQ=255)                                                                                                                                   cattGGCGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTACCAGGGTGCCCACTTTAGAGAAGTCAGGGAAGAAGGTCTTCGAATCCATTTCGATAGCAACGGGGGCACCGGAGTGc  <  2:148970/140‑1 (MQ=255)                                                                                                                                   cattGGCGTGGGTTGCGCATGCATCTACGCCGATACAACTCAAAATGATGGCAACAAATACTACCAGGGTGCCCACTTTAGAGAAGTCAGGGAAGAAGGTCTTCGAATCCATTTCGATAGCAACGGGGGCACCGGAGTGc  <  2:424779/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       GCGGAGAGGTTGATTTCATTACCCGGAATGACCATCGCAATAGACAAACCACCAACAGAGCTTAAGCAGATTGCCGCCACCATCAGCAGTAACATAGCCAACGGATAGTCGCGCCCTGGGTTGCTCATTTCATTGACGTGGGTTGCGGATGCTTCTACGCCCATATAACTCAAAATGAAGGCAACAAATACTACCAGGGTGCCCACTTTAGAGAAGTCAGGGAAGAAGGTCTTCGAATCCATTTCGATAGCAACGGGGGCACCGGAGTGC  >  NC_000913/1569684‑1569953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑