Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,619,930 A→G L119L (TTA→TTG)  marA → DNA‑binding transcriptional dual regulator MarA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,619,9300AG100.0% 282.5 / NA 79L119L (TTA→TTG) marADNA‑binding transcriptional dual regulator MarA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (43/36);  total (43/36)

AACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTCCGCGCAGGGCGTTGCGGAACAAACCAC  >  NC_000913/1619793‑1620062
                                                                                                                                         |                                                                                                                                    
aaCGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTGCa                                                                                                                                    <  1:1257094/140‑1 (MQ=255)
aaCGAGCCGATACTCTATCTGGCAGAACGATATGGATTCCAGTCGCAATAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTGCa                                                                                                                                    <  2:448653/140‑1 (MQ=255)
 aCGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGAGCGAATCGCGCTTTTTGCAt                                                                                                                                   >  1:110061/1‑140 (MQ=255)
    aGCCGATACTCTATCTGGCAGAACGATATGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTGCATCCa                                                                                                                                <  1:1444321/140‑1 (MQ=255)
     gCCGATACTCTATCTGGCAGAACGATATGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTGCATCCAt                                                                                                                               <  2:1367181/140‑1 (MQ=255)
       cGATACTCTATCTGGCAGAACGCTACGGCCTCGAGTCCCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTGCATCCATTa                                                                                                                             <  1:394804/140‑1 (MQ=255)
        gATACTCTATCTGGCAGCACGACATGGCTTCCAGTCGCATCAAACTCTCACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTGCATCCATTaa                                                                                                                            <  2:393409/140‑1 (MQ=255)
        gATACTCTATCTGACAGAACGATATGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAGTCGCGCTTTTTGCATCCATTaa                                                                                                                            <  1:792087/140‑1 (MQ=255)
                      cAGAACGATATGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCTGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGc                                                                                                              >  1:1373856/1‑140 (MQ=255)
                      cAGAACGATATGGCTGCGAGTCGCATCAAACTCTGACTCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGc                                                                                                              <  1:744490/140‑1 (MQ=255)
                       aGAACGATATGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCt                                                                                                             >  2:777863/1‑140 (MQ=255)
                         aaCGATATGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATGGCGCTTTCTGCGTCTATTAAAACATTACAACAGCTAg                                                                                                           >  1:504446/1‑140 (MQ=255)
                         aaCGATATGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATACAGGGCGAATCGGGCTCTTTGCATCCATTAGATCATTACAACAGCTAg                                                                                                           >  2:602161/1‑140 (MQ=255)
                           cGATATGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGtt                                                                                                         >  1:1324599/1‑140 (MQ=255)
                           cGATATGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGtt                                                                                                         <  2:1061076/140‑1 (MQ=255)
                           cGATATGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGACGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGtt                                                                                                         >  1:958562/1‑140 (MQ=255)
                               atGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGaaa                                                                                                     >  2:975479/1‑140 (MQ=255)
                                   cTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGt                                                                                                 >  1:145230/1‑140 (MQ=255)
                                   cTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGt                                                                                                 <  2:145230/140‑1 (MQ=255)
                                     tCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGa                                                                                               >  2:897286/1‑140 (MQ=255)
                                     tCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGAGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGa                                                                                               <  2:110061/140‑1 (MQ=255)
                                      cGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGAc                                                                                              <  1:1060788/140‑1 (MQ=255)
                                        aGTCGCAACAAACTCTGTCCCGGACCTTCAACAATTACTATGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACaa                                                                                            <  2:504446/140‑1 (MQ=255)
                                        aGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGtt                                                                                                         <  2:1158775/127‑1 (MQ=255)
                                        aGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGtt                                                                                                         >  1:1158775/1‑127 (MQ=255)
                                             caacaaACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGAATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTATTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCa                                                                                       >  1:1378657/1‑140 (MQ=255)
                                                   aCTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAgg                                                                                 >  1:1069028/1‑140 (MQ=255)
                                                    ctctGACCCGAACCTTCAAAAATTACTTTGATGTTCCGACGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGc                                                                                <  1:1258094/140‑1 (MQ=255)
                                                        gACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATc                                                                            >  2:1253488/1‑140 (MQ=255)
                                                        gACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATc                                                                            >  1:1166324/1‑140 (MQ=255)
                                                           ccGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATg                                                                         <  1:897286/140‑1 (MQ=255)
                                                              aaCCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGaaa                                                                      <  2:1324599/140‑1 (MQ=255)
                                                               aCCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAAc                                                                     >  1:1407238/1‑140 (MQ=255)
                                                                   tCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACt                                                                 >  2:223077/1‑140 (MQ=255)
                                                                   tCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACt                                                                 >  2:1397465/1‑140 (MQ=255)
                                                                       aaaTTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCa                                                             <  1:1489220/140‑1 (MQ=255)
                                                                        aaTTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCAt                                                            <  1:387611/140‑1 (MQ=255)
                                                                             cTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCa                                                       >  1:841031/1‑140 (MQ=255)
                                                                               ttGATGTTCCGCCGCATAAATACCGCATGCCCAATATTCAGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAAt                                                     <  1:396985/140‑1 (MQ=255)
                                                                                       ccgccgCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTg                                             <  2:1407238/140‑1 (MQ=255)
                                                                                        cgccgcATAAATACCGGATGACCAATATGTAGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATTAAATCACTTTCATGCGCAATAGCAGCTgc                                            >  1:649244/1‑140 (MQ=255)
                                                                                          ccgcATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTgcgc                                          >  1:1012749/1‑140 (MQ=255)
                                                                                            gcATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCtt                                        <  2:1228657/140‑1 (MQ=255)
                                                                                             cATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTa                                       <  2:841031/140‑1 (MQ=255)
                                                                                               tAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTAtt                                     <  2:1069028/140‑1 (MQ=255)
                                                                                                  aTACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATtctc                                  >  2:1517238/1‑140 (MQ=255)
                                                                                                       ggATGACCAATATGCAGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTgcgc                                          >  2:1533160/1‑127 (MQ=255)
                                                                                                       ggATGACCAATATGCAGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTgcgc                                          <  1:1533160/127‑1 (MQ=255)
                                                                                                       ggATGACCAATATGCAGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTc                             >  1:1133102/1‑140 (MQ=255)
                                                                                                        gATGACCAATATGCAGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTcc                            >  1:348071/1‑140 (MQ=255)
                                                                                                          tGACCAATATGCAGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTCcgc                          >  1:1498014/1‑140 (MQ=255)
                                                                                                           gACCAATATGCAGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTCcgcg                         <  1:777863/140‑1 (MQ=255)
                                                                                                           gACCAATATGCAGGGAGAATCGAGCCTTTTGCATCCAGTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTCcgcg                         <  1:602161/140‑1 (MQ=255)
                                                                                                            aCCAATATGCAGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTCCgcgc                        >  1:1040516/1‑140 (MQ=255)
                                                                                                            aCCAATATGCAGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTACATCCGCCATAGCAGCCGCGCCTATTCTCTTTTCCgcgc                        >  2:709239/1‑140 (MQ=255)
                                                                                                             ccAATATGCAGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTCCGCGCa                       >  1:256014/1‑140 (MQ=255)
                                                                                                                atatGCAGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTCCGCGCAggg                    <  2:1012749/140‑1 (MQ=255)
                                                                                                                atatGCAGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTCCGCGCAggg                    <  2:256014/140‑1 (MQ=255)
                                                                                                                atatACAGGGCGAATCGCGCTTGTTGCATCCATTATATCTTTACAACATCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTAATCTCTTTTCCGCGCAggg                    <  2:649244/140‑1 (MQ=255)
                                                                                                                    gCAGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTCCGCGCAGGGCGtt                <  1:1517238/140‑1 (MQ=255)
                                                                                                                    gCAGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTCCGCGCAGGGCGtt                >  1:1111913/1‑140 (MQ=255)
                                                                                                                     cAGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTCCGCGCAGGGCGTTg               <  1:1466296/140‑1 (MQ=255)
                                                                                                                     cAGGGCGAATCGCGCATTTTGCGTCGATTAAATCATTGCAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTCCGCGCAGGGCGTTg               <  1:709239/140‑1 (MQ=255)
                                                                                                                      aGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTGATCCGCAACAGCTGCTGCGCTTATTCTCTTTTCCGCGCAGGGCGTTga              >  2:647041/1‑139 (MQ=255)
                                                                                                                      aGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATATCAGCTGCTCTTCTTGTCTTTTGCGCGCAGGGCGTTGc              >  1:403473/1‑140 (MQ=255)
                                                                                                                      aGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTCCGCGCAggg                    >  2:1055273/1‑134 (MQ=255)
                                                                                                                      aGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTCCGCGCAggg                    <  1:1055273/134‑1 (MQ=255)
                                                                                                                      aGGGCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTCCGCGCAGGGCGTTGc              <  2:1178643/140‑1 (MQ=255)
                                                                                                                      aGGGCGAATCGCGCTGTTTGCAGCCATTATATCATTATAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTCCGCGCAGGGCGTTGc              <  1:667962/140‑1 (MQ=255)
                                                                                                                       gggCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTCCGCGCAGGGCGTTGCg             >  2:87545/1‑140 (MQ=255)
                                                                                                                        ggCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCTCAATAGCAGCTGCGCTTATTCTCTTTTCCGCGCATGTCGTTGCgg            >  1:953567/1‑140 (MQ=255)
                                                                                                                        ggCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTCCGCGCAGGGCGTTGCgg            >  2:9029/1‑140 (MQ=255)
                                                                                                                        ggCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTCCGCGCAGGGCGTTGCgg            >  2:969250/1‑140 (MQ=255)
                                                                                                                         gCGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCCGCGGTTATTCTCATTTCCGCGCAGGGCGTTGCGGa           >  2:586408/1‑140 (MQ=255)
                                                                                                                          cGAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTCCGCGCAGGGCGTTGCGGaa          >  1:74880/1‑140 (MQ=255)
                                                                                                                           gAATCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCACAATAGCAGCTGAGCTAATTCTCTTGTCCGCGCAGGGCGTTGCGGAAc         >  2:459566/1‑140 (MQ=255)
                                                                                                                            aaTCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTCCGCGCAGGGCGTTGCGGAACa        <  1:1397465/140‑1 (MQ=255)
                                                                                                                            aaTCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTCCGCGCAGGGCGTTGCGGAACa        <  2:348071/140‑1 (MQ=255)
                                                                                                                             aTCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCCATAGCAGCCGCGCTTAGTCTCTTTTCCGCGTAGGGCGTTGCGGAACaa       >  2:683225/1‑140 (MQ=255)
                                                                                                                             aTCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTCCGCGCAGGGCGTTGCGGAACaa       >  2:1456804/1‑140 (MQ=255)
                                                                                                                             aTCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTCCGCGCAGGGCGTTGCGGAACaa       >  2:1177623/1‑140 (MQ=255)
                                                                                                                             aTCGCGCTTTTTGCATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCGGCGGTTACTCTCTTTTTCGCGAAGGGCGTTGCGGAACaa       >  2:433286/1‑140 (MQ=255)
                                                                                                                                  gcTTTTTGCATCCATCAAATCACTACAACCGCTAGTTTAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTCCGCGCAGGGCGTTGCGGAACAAACCAc  <  1:459566/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                    
AACGAGCCGATACTCTATCTGGCAGAACGATATGGCTTCGAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTCCGCGCAGGGCGTTGCGGAACAAACCAC  >  NC_000913/1619793‑1620062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑