Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	215,141	(A)8→7	coding (309/423 nt)	arfB →	peptidyl‑tRNA hydrolase, ribosome rescue factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	215,134	0	A	.	100.0%	212.2 / NA	50	coding (302/423 nt)	arfB	peptidyl‑tRNA hydrolase, ribosome rescue factor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (26/24);  total (26/24)

CGTCTGCTCGCCGCCAGCCATCATTTGATCAGCAGTGATGGCGTGATTGTCATTAAGGCACAGGAATACCGCAGTCAGGAACTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAGAAAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGAAGGA  >  NC_000913/214995‑215264                                                                                                                                            |                                                                                                                                   cGTCTGCTCGCCGCCAGCCATCATTTGATCAGCAGTGATGGCGTGATTGTCATTAAGGCACAGGAATACCGCAGTCAGGAACTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGACTCTGATTATAGAATTAACAACAGa                                                                                                                                    >  1:425695/1‑140 (MQ=255)   tCTGCTCGCCGCCAGCCATCATTTGATCAGCAGTGATGGCGTGATTGTCATTAAGGCACAGGAATACCGCAGTCAGGAACTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAGaaa                                                                                                                                  >  1:110860/1‑140 (MQ=255)    cTGCTCGCCGCCAGCCATCATTTGATCAGCAGTGATGGCGTGATTGTCATTAAGGCACAGGAATACCGCAGTCAGGAACTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAGaaaa                                                                                                                                 >  1:225051/1‑140 (MQ=255)       cTCGCCGCCAGCCATCATTTGATCAGCAGTGATGGCGTGATTGTCATTAAGGCACAGGAATACCGCAGTCAGGAACTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTAAGATTAAAGAATTAACAACAGaaaaaaa                                                                                                                              <  1:796297/140‑1 (MQ=255)            cgccAGCCATCATTTGATCAGCAGTGATGGCGTGATTGTCATTAAGGCACAGGAATACCGCAGTCAGGAACTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAG‑AAAAAAAGCCcg                                                                                                                        >  2:190676/1‑140 (MQ=255)                 gccaTCATTTGATCAGCAGTGATGGCGTGATTGTCATTAAGGCACAGGAATACCGCAGTCAGGAACTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAG‑AAAAAAAGCCcgac                                                                                                                      >  2:884496/1‑137 (MQ=255)                 gccaTCATTTGATCAGCAGTGATGGCGTGATTGTCATTAAGGCACAGGAATACCGCAGTCAGGAACTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAG‑AAAAAAAGCCcgac                                                                                                                      <  1:884496/137‑1 (MQ=255)                 gccaTCATTTGATCAGCAGTGATGGCGTGATTGTCATTAAGGCACAGGAATACCGCAGTCAGGAACTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAG‑AAAAAAAGCCCgacgac                                                                                                                   >  1:944772/1‑140 (MQ=255)                     tcatTTGATCAGCAGTGATGGCGTGATTGTCATTAAGGCACAGGAATACTGCAGTCAGGAACTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAG‑AAAAAAAGCCCGACGACCCAc                                                                                                               <  1:659613/140‑1 (MQ=255)                         ttGATCAGCAGTGATGGCGTGATTGTCATTAAGGCACAGGAATACCGCAGTCAGGAACTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAG‑AAAAAAAGCCCGACGACCCACGCgg                                                                                                           <  2:36720/140‑1 (MQ=255)                           gATCAGCAGTGATGGCGTGATTGTCATTAAGGCACAGGAATACCGCAGTCAGGAACTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAG‑AAAAAAAGCCCGACGACCCACGCGGcc                                                                                                         <  2:554094/140‑1 (MQ=255)                                gcagTGATGGCGTGATTGTCATTAAGGCACAGGAATACCGCAGTCAGGAACTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAG‑AAAAAAAGCCCGACGACCCAc                                                                                                               >  2:619520/1‑129 (MQ=255)                                gcagTGATGGCGTGATTGTCATTAAGGCACAGGAATACCGCAGTCAGGAACTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAG‑AAAAAAAGCCCGACGACCCAc                                                                                                               <  1:619520/129‑1 (MQ=255)                                 cagTGATGGCGTGATTGTCATTAAGGCACAGGAATACCGCAGTCAGGAACTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAG‑AAAAAAAGCCCGACGACCCACGCGGCCCACCCg                                                                                                   >  1:109092/1‑140 (MQ=255)                                  agTGATGGCGTGATTGTCATTAAGGCACAGGAATACCGCAGTCAGGAACTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAG‑AAAAAAAGCCCGACGACCCACGCGGCCCACCCGt                                                                                                  >  2:896197/1‑140 (MQ=255)                                     gATGGCGTGATTGTCATTAAGGCACAGGAATACCGCAGTCAGGAACTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAG‑AAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCa                                                                                               <  2:978138/140‑1 (MQ=255)                                      aTGGCGTGATTGTCATTAAGGCACAGGAATACCGCAGTCAGGAACTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATTATTAAAGAATTAACAACAG‑AAAAAAAGCCCTACGACCCACGCGGCCCACCCGTGCAt                                                                                              >  1:963896/1‑140 (MQ=255)                                           gTGATTGTCATTAAGGCACAGGAATACCGCAGTCAGGAACTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAG‑AAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGaaa                                                                                         >  1:228944/1‑140 (MQ=255)                                           gTGATTGTCATTAAGGCACAGGAATACCGCAGTCAGGAACTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAG‑AAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGaaa                                                                                         <  2:254366/140‑1 (MQ=255)                                                       aaGGCACAGGAATACCGCAGTCAGGAACTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATTATTAAAGAATTAACAACAG‑AAAAAAAGCCCTACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTg                                                                             <  2:963896/140‑1 (MQ=255)                                                                   aaCCGCAGGCAGGAACTGAACCCCGAAGCAGCTTTGGCCTGGCTGGTGGCTATGATTAAAGAATTAACAACAG‑AAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGca                                                                 <  2:454560/139‑1 (MQ=255)                                                                    aCCGCAGTCAGGAACTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAGTTAACAACAG‑AAAAAAAGCCCGACGACCCGCGCGGCCCACCCGTGCGTCGAAAGATCGCAGGCTGGCATCGAAAGcaa                                                                >  2:258493/1‑139 (MQ=255)                                                                     ccGCAGTCAGGAACTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAG‑AAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGcaca                                                               <  2:228944/140‑1 (MQ=255)                                                                        cAGTCAGGAACTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAG‑AAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGCACaaaa                                                            >  1:965133/1‑140 (MQ=255)                                                                        cAGTCAGGAACTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAG‑AAAAAAAGCCCGACGACCCACGCGGCCCACCCGTACATCGAAGGAGTGCAGGCTGGCATCGAAAGCACaaaa                                                            >  1:469604/1‑140 (MQ=255)                                                                          gTCAGGAACTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAG‑AAAAAAAGCCCGACGACCCACGCGGTCCACCCGTACATTGAAAGAGCGCAGGCTGGGATCGAAAGCACAAAAAt                                                          >  2:331295/1‑140 (MQ=255)                                                                          gTCAGGAACTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAG‑AAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGCACAAAAAt                                                          >  1:1009540/1‑140 (MQ=255)                                                                           tCAGGAACTGAACCGCGAAGCAGCTTTGGCCCGGCTGATGGCTATGATTAAAGAATTAACAACAG‑AAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGCACAAAAATc                                                         <  1:370360/140‑1 (MQ=255)                                                                           tCAGGAACTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAG‑AAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGCACAAAAATc                                                         <  1:73475/140‑1 (MQ=255)                                                                                    gAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAG‑AAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTCGCATCGCAAGCACTAAAATCAAGCGTGaa                                                >  1:338010/1‑140 (MQ=255)                                                                                     aaCCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAG‑AAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATGGAGAGCACATAAATCAAGCGTGAAg                                               >  1:293640/1‑140 (MQ=255)                                                                                     aaCCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAG‑AAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAg                                               >  2:825985/1‑140 (MQ=255)                                                                                     aaCCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAG‑AAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGACCGCCGGCGGGCATCGACAGCACAAAAATCAAGCGTGAAg                                               >  2:370360/1‑140 (MQ=255)                                                                                        cgcgAAGCAGCTCTGTCCCGGCTGGTGGCTATGATTAGAGAATTAACAACAG‑AAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCg                                            <  1:331295/140‑1 (MQ=255)                                                                                        cgcgAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAG‑AAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCg                                            <  2:644221/140‑1 (MQ=255)                                                                                        cgcgAAGCAGCTCTGGCCCGGCTGGTGGCTATGATCAAATAATTAACAACAG‑AAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCg                                            <  2:362206/140‑1 (MQ=255)                                                                                         gcgAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAG‑AAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGa                                           >  1:853675/1‑140 (MQ=255)                                                                                          cgAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAG‑AAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGAt                                          <  1:944661/140‑1 (MQ=255)                                                                                               cagcTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAG‑AAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGcgcg                                     >  1:212328/1‑140 (MQ=255)                                                                                                  ctctGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAG‑AAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCa                                  >  2:975846/1‑140 (MQ=255)                                                                                                  ctctGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAG‑AAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCa                                  <  2:173253/140‑1 (MQ=255)                                                                                                      ggCCCGGCTGGTGGCTATGATTTAAGAATTATCAACAG‑AAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTTAAGGCGATGCGCGGCAAAGt                              <  2:425695/140‑1 (MQ=255)                                                                                                            gCTGGTGGCTATGATTAAAGAATTAACAACAG‑AAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGCACAAGAATCAAGGGTGAAGTCGATGCTCGGCAAAGTGCGCAg                        >  1:282535/1‑140 (MQ=255)                                                                                                                gtggCTATGATTAAAGAATTAACAACAG‑AAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGt                    <  2:944772/140‑1 (MQ=255)                                                                                                                 tggCTATGATTAAAGAATTAACAACAG‑AAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTc                   >  2:766340/1‑140 (MQ=255)                                                                                                                   gCTATGATTAAAGAATTAACAACAG‑AAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCgg                 <  2:85366/140‑1 (MQ=255)                                                                                                                        gATTAAAGAATTAACAACAG‑AAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATa            <  2:675629/140‑1 (MQ=255)                                                                                                                           tAAAGAATTAACAACAG‑AAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATaaaa         <  2:7725/140‑1 (MQ=255)                                                                                                                           tAAAGAAGTAACAACAG‑AAAAAGAGCCCGAGAACCGACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATaaaa         <  2:469604/140‑1 (MQ=255)                                                                                                                             aaGAATTAACAACAG‑AAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAaag       <  2:965133/140‑1 (MQ=255)                                                                                                                             aaGAATTAACAACAG‑AAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTAGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAaag       >  1:214592/1‑140 (MQ=255)                                                                                                                              aGAATTAACAACAG‑AAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAaaga      <  2:225051/140‑1 (MQ=255)                                                                                                                                  ttAACAACAG‑AAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGAAGGa  >  1:695002/1‑140 (MQ=255)                                                                                                                                  ttAACAACAG‑AAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGAAGGa  >  1:618888/1‑140 (MQ=255)                                                                                                                                  ttAACAACAG‑AAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAATGCGCAGCGGTCGGGAATAAAAAGAAGGa  >  1:450446/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                                   CGTCTGCTCGCCGCCAGCCATCATTTGATCAGCAGTGATGGCGTGATTGTCATTAAGGCACAGGAATACCGCAGTCAGGAACTGAACCGCGAAGCAGCTCTGGCCCGGCTGGTGGCTATGATTAAAGAATTAACAACAGAAAAAAAAGCCCGACGACCCACGCGGCCCACCCGTGCATCGAAAGAGCGCAGGCTGGCATCGAAAGCACAAAAATCAAGCGTGAAGGCGATGCGCGGCAAAGTGCGCAGCGGTCGGGAATAAAAAGAAGGA  >  NC_000913/214995‑215264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑