Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,592,406	Δ1 bp	coding (750/1656 nt)	tsr →	methyl‑accepting chemotaxis protein Tsr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,592,403	0	G	.	100.0%	190.7 / NA	45	coding (747/1656 nt)	tsr	methyl‑accepting chemotaxis protein Tsr
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (20/25);  total (20/25)

TGGCGGTCATCTTCGCCGTCTGGTTCGGTATTAAAGCCTCGCTGGTAGCGCCAATGAATCGCCTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGATGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGC  >  NC_000913/4592267‑4592536                                                                                                                                         |                                                                                                                                      tGGCGGTCATCTTCGCCGTCTGGTTCGGTATTAAAGCCTCGCTGGTAGCGCCAATGAATCGCCTGATTAACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGATgggc                                                                                                                                    <  2:248981/140‑2 (MQ=255) tGGCGGTCATCTTCGCCGTCTGGCTCGGTATTAAAGCCTCGCTGGTAGCGCCAATGAATCGCCTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGATgggc                                                                                                                                    <  1:162286/140‑2 (MQ=255)      gTCATCTTCGCCGTCTGGTTCGGTATTAAAACCTCGCTGGTAGCGCCAATGAATCGCCTGATTTACAGCATTCGTCATATTGCAGGCGGCGGTCTGGGGAAAACCATTGAGGGGGGTGGCTCTAATTAGAAGGGG‑AACTg                                                                                                                              >  2:669691/1‑140 (MQ=255)                 cGTCTGGTTCGGTATCAAAGCCTCGCTGGCAGCGCCACTGAATCGCCTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGttt                                                                                                                   <  1:264318/140‑1 (MQ=255)                     tGGTTCGGTATTAAAGCCTCGCTGGTAGCGCCAATGAATCGCCTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTgcgc                                                                                                               >  2:943968/1‑140 (MQ=255)                     tGGTTCGGTATTAAAGCCTCGCTGGTAGCGCCAATGAATCGCCTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTgcgc                                                                                                               <  2:572669/140‑1 (MQ=255)                      ggTTCGGTATTAAAGCCTCGCTGGTAGCGCCAATGAATCGCCTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGcc                                                                                                              <  1:220836/140‑1 (MQ=255)                      ggTTCGGTATTAAAGCCTCGCTGGTAGCGCCAATGAATCGCCTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGcc                                                                                                              <  1:550354/140‑1 (MQ=255)                       gTTCGGTATTAAAGCTTCGCTGTTAGCGCTAATGAATCGCCTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCa                                                                                                             <  1:367464/140‑1 (MQ=255)                       gTTCGGTATTAAAGCCTAGCTGGTAGCGCCAATGAATCGCCTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCa                                                                                                             <  2:406333/140‑1 (MQ=255)                            gTATTAAAGCCTCGCTGGTAGCTCCAATGAATCGCTTGAATGACAGCACTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGc                                                                                                        <  2:370112/140‑1 (MQ=255)                                 aaaGCCTCGCTGGTAGCGCCAATGAGTCGCCTGATTGTCAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGga                                                                                                   <  1:446780/140‑1 (MQ=255)                                    gCCTCGCTGGTAGCGCCAATGAATCGCCTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGagag                                                                                                <  1:165990/140‑1 (MQ=255)                                       tCGCTGGTAGCGCCAATGAATCGCCTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTg                                                                                             >  2:166159/1‑140 (MQ=255)                                         gCTGGTAGCGCCAATGAATCGCCTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATTAGAT‑GGGCGACTGGCATAGAGTTTGGGCCATATGCAGGGAGAGCTGAt                                                                                           >  2:312134/1‑140 (MQ=255)                                           tGGTAGCGCCAATGAATCGCCTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGc                                                                                         <  1:943968/140‑1 (MQ=255)                                               aGCGCCAATGAATCGCCTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTAc                                                                                     <  1:74595/140‑1 (MQ=255)                                               aGCGCCAATGAATCGCCTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTAc                                                                                     <  1:780057/140‑1 (MQ=255)                                                       tGAATCGCCTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCAAATGCAGGGAGAGCTGATGCGTACCGTCGGTg                                                                             >  2:419631/1‑140 (MQ=255)                                                            cGCCTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGAtgtg                                                                        >  1:924777/1‑140 (MQ=255)                                                                  aTTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAAc                                                                  <  1:166159/140‑1 (MQ=255)                                                                   ttGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTg                                                                             >  1:677912/1‑128 (MQ=255)                                                                   ttGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTg                                                                             <  2:677912/128‑1 (MQ=255)                                                                   ttGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACg                                                                 <  1:771360/140‑1 (MQ=255)                                                                       cAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGc                                                             <  1:669691/140‑1 (MQ=255)                                                                       cAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGc                                                             <  1:761993/140‑1 (MQ=255)                                                                            ttCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACgggg                                                              >  1:783872/1‑134 (MQ=255)                                                                            ttCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACgggg                                                              <  2:783872/134‑1 (MQ=255)                                                                              cGTCATATTGCAGGCGGCGATCGGGTGAACCCGATTGCGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATGcc                                                      <  1:453916/140‑1 (MQ=255)                                                                               gTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTAGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCGACGGGGCCAATGCCa                                                     >  1:448643/1‑140 (MQ=255)                                                                                      ggCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATGCCATCTATAg                                              <  1:822045/139‑1 (MQ=255)                                                                                          ggcggcGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATg                                                        <  1:144002/126‑1 (MQ=255)                                                                                          ggcggcGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATg                                                        >  2:144002/1‑126 (MQ=255)                                                                                             ggcgATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATGCCATCTATAGCGGTGcc                                       >  2:622432/1‑140 (MQ=255)                                                                                               cgATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAAGGGGTCCAATGCCAGCTATAGCGGTGCCAg                                     >  1:429805/1‑140 (MQ=255)                                                                                                     ggTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATGCCATCTATAGCGGTGCCAGCGAAAt                               >  1:877041/1‑140 (MQ=255)                                                                                                     ggTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATGCCATCTATAGCGGTGCCAGCGAAAt                               >  1:227379/1‑140 (MQ=255)                                                                                                            ccGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCAcc                        <  2:503051/140‑1 (MQ=255)                                                                                                              gATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGGCAATGCCCTCTATAGCGGTGCCAGCGAAATCGCCACCgg                      >  2:459525/1‑140 (MQ=255)                                                                                                              gATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCgg                      <  1:645324/140‑1 (MQ=255)                                                                                                                    ggTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATGCCATCTATAGCGGTGCCAGTGAAATCGCCACCGGCAATaa                <  1:604349/140‑1 (MQ=255)                                                                                                                    ggTGGATGGCTCTAATGAGAT‑GCGCAACTGGGAGAAAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATaa                <  2:429805/140‑1 (MQ=255)                                                                                                                         aTGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGAtc           >  2:603096/1‑140 (MQ=255)                                                                                                                             ctctAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGAtctctc       >  2:879988/1‑140 (MQ=255)                                                                                                                             ctctAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATGCCATCTATAGCAGTGCCAGTGAAATCGCCACCGGCAATAACGAtctctc       >  1:446816/1‑140 (MQ=255)                                                                                                                              tctAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATctctct      >  1:982140/1‑140 (MQ=255)                                                                                                                                  aTGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTcgc  >  2:535765/1‑140 (MQ=255)                                                                                                                                  aTGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTcgc  >  2:893659/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      TGGCGGTCATCTTCGCCGTCTGGTTCGGTATTAAAGCCTCGCTGGTAGCGCCAATGAATCGCCTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGATGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGC  >  NC_000913/4592267‑4592536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑