Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,510,788	(A)8→7	coding (786/1407 nt)	ydcR →	fused putative DNA‑binding transcriptional regulator/putative aminotransferase YdcR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,510,781	0	A	.	100.0%	182.3 / NA	43	coding (779/1407 nt)	ydcR	fused putative DNA‑binding transcriptional regulator/putative aminotransferase YdcR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (20/23);  total (20/23)

AAGGCGTTATCGGTGGCGACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAGATGACGTTTACAGCGAACTTTATTTTGGACGGGAAAAACCGCTGCCTGCGAAAGCGTGGGATCGCCACGATGGCGTT  >  NC_000913/1510642‑1510914                                                                                                                                            |                                                                                                                                      aaGGCGTTATCGGTGGCGACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCa                                                                                                                                       >  2:68820/1‑140 (MQ=255)  aGGCGTTATCGGTGGCGACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCaa                                                                                                                                      >  1:210320/1‑140 (MQ=255)                gCGACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCaaaaaaagcac                                                                                                                             <  2:665323/135‑5 (MQ=255)                gCGACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCaaaaaaagcac                                                                                                                             >  1:665323/1‑131 (MQ=255)                      gATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGC‑AAAAAAAGCACAACTGGTGGCg                                                                                                                 <  1:118547/140‑1 (MQ=255)                          ttAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGC‑AAAAAAAGCACAACTGGTGGCGTTGc                                                                                                             <  2:222714/140‑1 (MQ=255)                             aagaagGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGC‑AAAAAAAGCACAACTGGTGGCGTTGCTCa                                                                                                          <  2:197835/140‑1 (MQ=255)                               gaagGGATAGATCTTCATGCGCGGGAACTGGCGTTTCAGGAGTATCCGCTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGC‑AAAAAAAGCACAACTGGTGGCGTTGCTCAAt                                                                                                        <  2:413882/140‑1 (MQ=255)                                 agGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGC‑AAAAAAAGCACAACTGGTGGCGTTGCTCAATCa                                                                                                      <  1:838370/140‑1 (MQ=255)                                   ggATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGC‑AAAAAAAGCACAACTGGTGGCGTTGCTCa                                                                                                          >  2:991104/1‑134 (MQ=255)                                   ggATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGC‑AAAAAAAGCACAACTGGTGGCGTTGCTCa                                                                                                          <  1:991104/134‑1 (MQ=255)                                    gATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCTC‑AAAAAAAGCAAAATTGGTGGAGTTGCTCAATCAGTa                                                                                                   >  1:420818/1‑140 (MQ=255)                                     aTAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGC‑AAAAAAAGCACAACTGGTGGCGTTGCTCAAt                                                                                                        <  1:577433/134‑1 (MQ=255)                                     aTAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGC‑AAAAAAAGCACAACTGGTGGCGTTGCTCAAt                                                                                                        >  2:577433/1‑134 (MQ=255)                                      tAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGC‑AAAAAAAGCACAACTGGTGGCGTTGCTCAAt                                                                                                        >  2:808916/1‑133 (MQ=255)                                      tAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGC‑AAAAAAAGCACAACTGGTGGCGTTGCTCAAt                                                                                                        <  1:808916/133‑1 (MQ=255)                                          tCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGC‑AAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGt                                                                                             >  2:150322/1‑140 (MQ=255)                                          tCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGC‑AAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGt                                                                                             <  2:248903/140‑1 (MQ=255)                                             tCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGC‑AAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAAc                                                                                          <  1:68820/140‑1 (MQ=255)                                              cAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGC‑AAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACg                                                                                         >  2:539124/1‑140 (MQ=255)                                              cAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGC‑AAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACg                                                                                         >  2:53229/1‑140 (MQ=255)                                              cAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGC‑AAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACg                                                                                         >  1:685125/1‑140 (MQ=255)                                              cAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGC‑AAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACg                                                                                         >  2:525297/1‑140 (MQ=255)                                               aGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGC‑AAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGc                                                                                        >  1:630985/1‑140 (MQ=255)                                                        aaCTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGC‑AAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAg                                                                               <  1:76680/140‑1 (MQ=255)                                                        aaCTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGC‑AAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAg                                                                               <  1:539124/140‑1 (MQ=255)                                                                     ggAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGC‑AAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAGATGACGTTTACAg                                                                  <  2:210320/140‑1 (MQ=255)                                                                     ggAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGC‑‑AAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAGATGACGTTTACAGc                                                                 <  2:814688/140‑1 (MQ=255)                                                                           tCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGC‑AAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAGATGACGTTTACAGCGAACt                                                            >  1:215786/1‑140 (MQ=255)                                                                           tCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGC‑AAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAGATGACGTTTACAGCGAACt                                                            >  2:43910/1‑140 (MQ=255)                                                                                   aaGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGC‑AAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAGATGACGTTTACAGCGAACTTTATTTTg                                                    >  2:783474/1‑140 (MQ=255)                                                                                    aGCGTGCTGGCTGATGAATAATAGCCAGAATGCACCCGGTTTTACCTTTACGCCGC‑AAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAGATGACGTTTACAGCGAACTTTATTTTgg                                                   <  2:420818/140‑1 (MQ=255)                                                                                     gCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGC‑AAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAGATGACGTTTACAGCGAACTTTATTTTGGa                                                  <  2:215786/140‑1 (MQ=255)                                                                                     gCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGC‑AAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAGATGACGTTTACAGCGAACTTTATTTTGGa                                                  <  1:881320/140‑1 (MQ=255)                                                                                      cGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGC‑AAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAGATGACGTTTACAGCGAACTTTATTTTGGAc                                                 <  2:630985/140‑1 (MQ=255)                                                                                         gctggctgATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGC‑AAAAAAAGCACAACTGGTGGCGTTGCTCAAGCAGTACAACGTAACGCTGATTGAAGATGACGTTTACAGCGAACTTTATTTTGGACggg                                              <  2:685125/140‑1 (MQ=255)                                                                                                       aaTAGCCAGAATCCACTCGGATTTACCTTAACGCCGC‑AAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAGATGACGTTTACAGCGAACTTTATTTTGGACGGGAAAAACCGctgcct                                <  1:150322/140‑1 (MQ=255)                                                                                                       aaTAGCCAGAATCCACTCGGATTTACCTTAACGCCGC‑AAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAGATGACGTTTACAGCGAACTTTATTTTGGACGGGAAAAACCGctgcct                                >  1:909295/1‑140 (MQ=255)                                                                                                       aaTAGCCAGAATCCACTCGGATTTACCTTAACGCCGC‑AAAAAAAGCACAACTGGCGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAGATGACGTTTACAGCGAACTTTATTTTGGACGGGAAAAACCGctgcct                                >  2:155186/1‑140 (MQ=255)                                                                                                           gCCAGAATCCACTCGGATTTACCTTAACGCCGC‑AAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAGATGACGTTTACAGCGAACTTTATTTTGGACGGGAAAAACCGCTGCCTGCGa                            <  2:909295/140‑1 (MQ=255)                                                                                                                        cGGATTTACCTTAACGCCGC‑AAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAGATGACGTTTACAGCGAACTTTATTTTGGACGGGAAAAACCGCTGCCTGCGAAAGCGTGGGATCg               >  1:192995/1‑140 (MQ=255)                                                                                                                           aTTTACCTTAACGCCGC‑AAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAGATGACGTTTACAGCGAACTTTATTTTGGACGGGAAAAACCGCTGCCTGCGAAAGCGTGGGATCGCCa            >  2:61460/1‑140 (MQ=255)                                                                                                                                ccTTAACGCCGC‑AAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAGATGACGTTTACAGCGAACTTTATTTTGGACAGGAAAAACCGCTGCCTGCGAAAGCGTGGGATCGCCACGATg       <  1:53229/140‑1 (MQ=255)                                                                                                                                     aCGCCGC‑AAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAGATGACGTTTACAGCGACCTTTATTTTGGACGGGAAAAACCGCTGCCTGCGAAAGCGTGGGATCg               <  1:722772/127‑1 (MQ=255)                                                                                                                                     aCGCCGC‑AAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAGATGACGTTTACAGCGAACTTTATTTTGGACGGGAAAAACCGCTGCCTGCGACAGAGTGGGATCGCCACGATGGCGtt  >  1:463346/1‑140 (MQ=255)                                                                                                                                     aCGCCGC‑AAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAGATGACGTTTACAGCGAACTTTATTTTGGACGGGAAAAACCGCTGCCTGCGAAAGCGTGGGATCg               >  2:722772/1‑127 (MQ=255)                                                                                                                                     aCGCCGC‑AAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAGATGACGTTTACAGCGAACTTTATTTTGGACGGGAAAAACCGCTGCCTGCGAAAGCGTGGGATCGCCACGATGGCGtt  >  1:178112/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                                      AAGGCGTTATCGGTGGCGACGGATGTTAAAGAAGGGATAGATCTTCAGGCGCTGGAACTGGCGTTGCAGGAGTATCCGGTGAAAGCGTGCTGGCTGATGACTAATAGCCAGAATCCACTCGGATTTACCTTAACGCCGCAAAAAAAAGCACAACTGGTGGCGTTGCTCAATCAGTACAACGTAACGCTGATTGAAGATGACGTTTACAGCGAACTTTATTTTGGACGGGAAAAACCGCTGCCTGCGAAAGCGTGGGATCGCCACGATGGCGTT  >  NC_000913/1510642‑1510914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑