| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,901,309 | (C)6→7 | coding (277/1557 nt) | yoaE ← | putative inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,901,303 | 1 | . | C | 94.7% | 126.4 / 0.4 | 38 | coding (283/1557 nt) | yoaE | putative inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base . (2/0); new base C (20/16); total (22/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.99e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.73e-01 | |||||||||||
AACGAGAAGACGGCGTCAAGGATGACAATCTGTGTGACGACCACCCAGAAACTGGCGTAGCCTTTACCGTGGCCGGAATCATGATCGCGGTTTTCCAGCCGTTCATGCAGTTCGGTTGTTGCTTTGAACAGCAAGAATA‑T‑CCCCCC‑GAACAACATAATCAGGTCGCGTCCGGAGAAGGAGAAATCCATGACGGTAAATAGCGGTTTGGTCAGCGTGACCATCCATGAAATCAGCGACAGCAGCCCCAGACGCATAATCAGCGCCAGTGATAAC > NC_000913/1901164‑1901436 | aaCGAGAAGACGGCGTCAAGGATGACAATCTGTGTGACGACCACCCAGAAACTGGCGTAGCCTTTACCGTGGCCGGAATCATGATCGCGGTTTTCCAGCCGTTCATGCAGTTCGGTTGTTGCTTTGAACAGCAAGAata‑t < 2:545531/140‑1 (MQ=255)aaCGAGAAGACGGCGTCAAGGATGACAATCTGTGTGACGACCACCCAGAAACTGGCGTAGCCTTTACCGTGGCCGGAATCATGATCGCGGTTTTCCAGCCGTTCATGCAGTTCGGTTGTTGCTTTGAACAGCAAGAata‑t < 2:371052/140‑1 (MQ=255) gaagaCGGCGTCAAGGATGACAATCTGTGTGACGACCACCCAGAAACTGGCGTAGCCTTTACCGTGGCCGGAATCATGATCGCGGTTTTCCAGCCGTTCATGCAGTTCGGTTGTTGCTTTGAACAGCAAGAATA‑T‑ccccc < 1:1006327/140‑1 (MQ=255) aCGGCGTCAAGGATGACAATCTGTGTGACGACCACCCAGAAACTGGCGTAGCCTTTACCGTGGCCGGAATCATGATCGCGGTTTTCCAGCCGTTCATGCAGTTCGGTTGTTGCTTTGAACAGCAAGAATA‑T‑cccccccga < 2:172170/140‑4 (MQ=255) cGTCAAGGATGACAACCTGTGTCACCACCCCCCAGAACCTGGCGTAGCCTTTACCGTGGCCGGAATCATGATCGCGGTTTTCCAGCCGTTCATGCAGTTCGGTTGTTGCTTTGAACAGCAAGAATA‑TCCCCCCC‑Gaacaa < 1:277516/140‑1 (MQ=255) aGGATGACAATCTGTGTGACGACCACCCAGAAACTGGCGTAGCCTTTACCGTGGCCGGAATCATGATCGCGGTTTTCCAGCCGTTCATGCAGTTCGGTTGTTGCTTTGAACAGCAAGTata‑t > 2:486371/1‑122 (MQ=255) aGGATGACAATCTGTGTGACGACCACCCAGAAACTGGCGTAGCCTTTACCGTGGCCGGAATCATGATCGCGGTTTTCCAGCCGTTCATGCAGTTCGGTTGTTGCTTTGAACAGCAAGAata‑t < 1:486371/122‑1 (MQ=255) aTCTGTGTGACGACCACCCAGAAACTGGCGTAGCCTTTACCGTGGCCGGAATCATGATCGCGGTTTTCCAGCCGTTCATGCAGTTCGGTTGTTGCTTTGAACAGCAAGAATA‑TCCCCCCC‑GAACAACATAATCAGGTcgc > 1:571107/1‑140 (MQ=255) tgtgtgACGACCACCCAGAAACTGGCGTAGCCTTTACCGTGGCCGGAATCATGATCGCGGTTTTCCAGCCGTTCATGCAGTTCGGTTGTTGCTTTGAACAGCAAGAATA‑TCCCCCCC‑GAACAACATAATCAGGTCGCGTc > 2:65633/1‑140 (MQ=255) tgtgtgACGACCACCCAGAAACTGGCGTAGCCTTTACCGTGGCCGGAATCATGATCGCGGTTTTCCAGCCGTTCATGCAGTTCGGTTGTTGCTTTGAACAGCAAGAATA‑TCCCCCCC‑GAACAACATAATCAGGTCGCGTc > 2:214636/1‑140 (MQ=255) gtgtgACGACCACCCAGAAACTGGCGTAGCCTTTACCGTGGCCGGAATCATGATCGCGGTTTTCCAGCCGTTCATGCAGTTCGGTTGTTGCTTTGAACAGCAAGAATA‑TCCCCCCC‑GAACAACATAATCAGGTCGCGTcc > 1:983679/1‑140 (MQ=255) gacgacCACCCAGAAACTGGCGTAGCCTTTACCGTGGCCGGAATCATGATCGCGGTTTTCCAGCCGTTCATGCAGTTCGGTTGTTGCTTTGAACAGCAAGAATA‑TCCCCCCC‑GAACAACATAATCAGGTCGCGTCCggag < 2:764823/140‑1 (MQ=255) acgacCACCCAGAAACTGGCGTAGCCTTTACCGTGGCCGGAATCATGATCGCGGTTTTCCAGCCGTTCATGCAGTTCGGTTGTTGCTTTGAACAGCAAGAATA‑TCCCCCCC‑GAACAACATAATCAGGTCGCGTCCggaga > 2:185024/1‑140 (MQ=255) acgacCACCCAGAAACTGGCGTAGCCTTTACCGTGGCCGGAATCATGATCGCGGTTTTCCAGCCGTTCATGCAGATCGGTTGTCGCTTTGAACAGCAAGAATA‑TCCCCCCC‑GAACAACATAATCAGGTCGCGTCCggaga > 1:708291/1‑140 (MQ=255) gacCACCCAGAAACTGGCGTAGCCTTTACCGTGGCCGGAATCATGATCGCGGTTTTCCAGCCGTTCATGCAGTTCGGTTGTTGCTTTGAACAGCAAGAATA‑TCCCCCCC‑GAACAACATAATCAGGTCGCGTCCggagaag > 2:576331/1‑140 (MQ=255) accCAGAAACTGGCGTAGCCTTTACCGTGGCCGGAATCATGATCGCGGTTTTCCAGCCGTTCATGCAGTTCGGTTGTTGCTTTGAACAGCAAGAATA‑TCCCCCCC‑GAACAACATAATCAGGTCGCGTCCggagaaggaga > 2:511668/1‑140 (MQ=255) aGAAACTGGCGTAGCCTTTACCGTGGCCGGAATCATGATCGCGGTTTTCCAGCCGTTCATGCAGTTCGGTTGTTGCTTTGAACAGCAAGAATA‑TCCCCCCC‑GAACAACATAATCAGGTCGCGTCCGGAGAAGGAGAAATc < 1:642754/140‑1 (MQ=255) aCTGGCGTAGCCTTTACCGTGGCCGGAATCATGATCGCGGTTTTCCAGCCGTTCATGCAGTTCGGTTGTTGCTTTGAACAGCGAGAATA‑TCCCCCCC‑GAACAACATAATCAGGTCGCGTCCGGAGAAGGAGAAATCCATg > 2:611123/1‑140 (MQ=255) aCTGGCGTAGCCTTTACCGTGGCCGGAATCATGATCGCGGTTTTCCAGCCGTTCATGCAGTTCGGTTGTTGCTTTGAACAGCAAGAATA‑TCCCCCCC‑GAACAACATAATCAGGTCGCGTCCGGAGAAGGAGAAATCCATg < 2:571107/140‑1 (MQ=255) ggCGTAGCCTTTACCGTGGCCGGAATCATGATCGCGGTTTTCCAGCCGTTCATGCAGTTCGGTTGTTGCTTTGAACAGCAAGAATA‑TCCCCCCC‑GAACAACATAATCAGGTCGCGTCCGGAGAAGGAGAAATCCATGACg < 1:214636/140‑1 (MQ=255) ccTTTACCGTGGCCGGAATCATGATCGCGGTTTTCCAGCCGTTCATGCAGTTCGGTTGTTGCTTTGAACAGCAAGAATA‑TCCCCCCC‑GAACAACATAATCAGGTCGCGTCCGGAGAAGGAGAAATCCATGACGGTAAATa > 1:754084/1‑140 (MQ=255) ccTTTACCGTGGCCGGAATCATGATCGCGGTTTTCCAGCCGTTCATGCAGTTCGGTTGTTGCTTTGAACAGCAAGAATA‑TCCCCCCC‑GAACAACATAATCAGGTCGCGTCCGGAGAAGGAGAAATCCATGACGGTAAATa > 1:601407/1‑140 (MQ=255) ccTTTACCGTGGCCGGAATCATGATCGCGGTTTTCCAGCCGTTCATGCAGTTCGGTTGTTGCTTTGAACAGCAAGAATA‑TCCCCCCC‑GAACAACATAATCAGGTCGCGTCCGGAGAAGGAGAAATCCATGACGGTAAATa < 1:576331/140‑1 (MQ=255) tACCGTGGCCGGAATCATGATCGCGGTTTTCCAGCCGTTCATGCAGTTCGGTTGTTGCTTTGAACAGCAAGAATA‑T‑CCCCCCGGAACAACATAATCAGGTCGCGTCCGGAGAAGGAGAAATCCATGACGGTAAATAGCgg > 2:621470/1‑140 (MQ=255) ggCCGGAATCATGATCGCGGTTTTCCAGCCGTTCATGCAGTTCGGTTGTTGCTTTGAACAGCAAGAATA‑TCCCCCCC‑GAACAACATAATCAGGTCGCGTCCGGAGAAGGAGAAATCCATGACGGTAAATAGCGGTTTGGt > 2:860402/1‑140 (MQ=255) gCCGGAATCATGATCGCGGTTTTCCAGCCGTTCATGCAGTTCGGTTGTTGCTTTGAACAGCAAGAATA‑TCCCCCCC‑GAACAACATAATCAGGTCGCGTCCGGAGAAGGAGAAATCCATGACGGTAAATAGCGGTTTGGTc > 1:539756/1‑140 (MQ=255) gCCGGAATCATGATCGCGGTTTTCCAGCCGTTCATGCAGTTCGGTTGTTGCTTTGAACAGCAAGAATA‑TCCCCCCC‑GAACAACATAATCAGGTCGCGTCCGGAGAAGGAGAAATCCATGACGGTAAATAGCGGTTTGGTc > 2:665670/1‑140 (MQ=255) aaTCATGATCGCGGTTTTCCAGCCGTTCATGCAGTTCGGTTGTTGCTTTGAACAGCAAGAATA‑TCCCCCCC‑GAACAACATAATCAGGTCGCGTCCGGAGAAGGAGAAATCCATGACGGTAAATAGCGGTTTgg < 2:234467/133‑1 (MQ=255) aaTCATGATCGCGGTTTTCCAGCCGTTCATGCAGTTCGGTTGTTGCTTTGAACAGCAAGAATA‑TCCCCCCC‑GAACAACATAATCAGGTCGCGTCCGGAGAAGGAGAAATCCATGACGGTAAATAGCGGTTTgg > 1:234467/1‑133 (MQ=255) aTGATCGCGGTTTTCCAGCCGTTCATGCAGTTCGGTTGTTGCTTTGAACAGCAAGAATA‑TCCCCCCC‑GAACAACATAATCAGGTCGCGTCCGGAGAAGGAGAAATCCATGACGGTAAATAGCGGTTTGGTCAGCGTGAcc > 1:633788/1‑140 (MQ=255) aTGATCGCGGTTTTCCAGCCGTTCATGCAGTTCGGTTGTTGCTTTGAACAGCAAGAATA‑TCCCCCCC‑GAACAACATAATCAGGTCGCGTCCGGAGAAGGAGAAATCCATGACGGTAAATAGCGGTTTGGTCAGCGTGAcc < 2:539756/140‑1 (MQ=255) gcgGTTTTCCAGCCGTTCATGCAGTTCGGTTGTTGCTTTGAACAGCAAGAATA‑TCCCCCCC‑GAACAACATAATCAGGTCGCGTCCGGAGAAGGAGAAATCCATGACGGTAAATAGCGGTTTGGTCAGCGTGAccatccat > 1:896972/1‑140 (MQ=255) ttCCAGCCGTTCATGCAGTTCGGTTGTTGCTTTGAACAGCAAGAATA‑TCCCCCCC‑GAACAACATAATCAGGTCGCGTCCGGAGAAGGAGAAATCCATGACGGTAAATAGCGGTTTGGTCAGCGTGACCATCCATGAAATc > 2:1008932/1‑140 (MQ=255) cGTTCATGCAGTTCGGTTGTTGCTTTGAACAGCAAGAATA‑TCCCCCCC‑GAACAACATAATCAGGTCGCGTCCGGAGAAGGAGAAATCCATGACGGTAAATAGCGGTTTGGTCAGCGTGACCATCCATGAAATCAGCGAca < 2:165720/140‑1 (MQ=255) cATGCAGTTCGGTTGTTGCTTTGAACAGCAAGAATA‑TCCCCCCC‑GAACAACATAATCAGGTCGCGTCCGGAGAAGGAGAAATCCATGACGGTAAATAGCGGTTTGGTCAGCGTGACCATCCATGAAATCAGCGAcagcag > 1:502479/1‑140 (MQ=255) aGTTCGGTTGTTGCTTTGAACAGCAAGAATA‑TCCCCCCC‑GAACAACATAATCAGGTCGCGTCCGGAGAAGGAGAAATCCATGACGGTAAATAGCGGTTTGGTCAGCGTGACCATCCATGAAATCAGCGACAGCAGCCCCa < 2:601407/140‑1 (MQ=255) aGTTCGGTTGTTGCTTTGAACAGCAAGAATA‑TCCCCCCC‑GAACAACATAATCAGGTCGCGTCCGGAGAAGGAGAAATCCATGACGGTAAATAGCGGTTTGGTCAGCGTGACCATCCATGAAATCAGCGACAGCAGCCCCa < 1:511668/140‑1 (MQ=255) cGGTTGTTGCTTTGAACAGCAAGAATA‑T‑CCCCCC‑GAACAACATAATCAGGTCGCGTCCGGAGAAGGAGAAATCCATGACGGTAAATAGCGGTTTGGTCAGCGTGACCATCCATGAAATCAGCGACAGCAGCCCCAGACGc > 2:150564/1‑140 (MQ=255) gttgttGCTTTGAACTGCAAGACTAGC‑CCCCCC‑GAACGACATAATCAGGTCGCGTCCGGAGAAGGAGAAATCCATGACGGTAAATAGCGGTTTGGTCAGCGTGACCATCCATGAAATCAGCGACAGCAGCCCCAGACGCa < 1:471034/140‑1 (MQ=255) ttgCTTTGAACAGCAAGAATA‑TCCCCCCC‑GAACAACATAATCAGGTCGCGTCCGGAGAAGGAGAAATCCATGACGGTAAATAGCGGTTTGGTCAGCGTGAGCATGCATCTAATCAGCCACAGCGGCCCCAGACGCATAAt > 2:471034/1‑140 (MQ=255) tgCTTTGAACAGCAAGAATA‑TCCCCCCC‑GAACAACATAATCAGGTCGCGTCCGGAGAAGGAGAAATCCATGACGGTAAATAGCGGTTTGGTCAGCGTGACCATCCATGAAATCAGCGACAGCAGCCCCAGACGCATAATc < 1:170604/140‑1 (MQ=255) ttGAACAGCAAGAATA‑TCCCCCCC‑GAACAACATAATCAGGTCGCGTCCGGAGAAGGAGAAATCCATGACGGTAAATAGCGGTTTGGTCAGCGTGACCATCCATGAAATCAGCGACAGCAGCCCCAGACGCATAATCAgcg < 2:708291/140‑1 (MQ=255) tGAACAGCAAGAATA‑TCCCCCCC‑GAACAACATAATCAGGTCGCGGCCGGAGAAGGAGAAAACCATGGCCGTAAATAGCCGTTTGGTCAGCGTGGCCATCCATGAAATCAGCGACAGCAGCCCCAGACGCATAATCAgcgc < 2:836837/140‑1 (MQ=255) gAACAGCAAGAATA‑TCCCCCCC‑GAACAACATAATCAGGTCGCGTCCGGAGAAGGAGAAATCCATGACGGTAAATAGCGGTTTGGTCAGCGTGACCATCCATGAAATCAGCGACAGCAGCCCCAGACGCATAATCAGCGcc < 2:709664/140‑1 (MQ=255) cAAGAATA‑TCCCCCCC‑GAACAACATAATCAGGTCGCGTCCGGAGAAGGAGAAATCCATGACGGTAAATAGCGGTTTGGTCAGCGTGACCATCCATGAAATCAGCGACAGCAGCCCCAGACGCATAATCAGCGCCAGTGAt < 1:810056/140‑1 (MQ=255) gAATA‑TCCCCCCC‑GAACAACATAATCAGGTCGCGTCCGGAGAAGGAGAAATCCATGACGGTAAATAGCGGTTTGGTCAGCGTGACCATCCATGAAATCAGCGACAGCAGCCCCAGACGCATAATCAGCGCCAGTGATAAc < 1:185024/140‑1 (MQ=255) | AACGAGAAGACGGCGTCAAGGATGACAATCTGTGTGACGACCACCCAGAAACTGGCGTAGCCTTTACCGTGGCCGGAATCATGATCGCGGTTTTCCAGCCGTTCATGCAGTTCGGTTGTTGCTTTGAACAGCAAGAATA‑T‑CCCCCC‑GAACAACATAATCAGGTCGCGTCCGGAGAAGGAGAAATCCATGACGGTAAATAGCGGTTTGGTCAGCGTGACCATCCATGAAATCAGCGACAGCAGCCCCAGACGCATAATCAGCGCCAGTGATAAC > NC_000913/1901164‑1901436 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |