| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,202,629 | (G)6→7 | coding (2351/3633 nt) | yehI → | DUF4132 domain‑containing protein YehI |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,202,623 | 1 | . | G | 97.1% | 116.8 / ‑3.2 | 34 | coding (2345/3633 nt) | yehI | DUF4132 domain‑containing protein YehI |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (16/17); total (17/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
CATTTACCCGGCACGCATTCCAAAAGCCCCTGATTTCTGGCATCCGCAAATGTGGTCCAGGCCGCGCCTTATCACTAATAATCAAACTGTTACCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCA‑GGGGG‑GACGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCAGCTTTTGCCTGGGATCTCTTCACTGCGTGGCAACAAGCTGGTGCCCCCGCAAAAGACAACTGGG > NC_000913/2202486‑2202751 | cATTTACCCGGCACGCATTCCAAAAGCCCCTGATTTCTGGCATCCGCAAATGTGGTCCAGGCCGCGCCTTATCACTAATAATCAAACTGTTACCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCA‑gg > 2:229713/1‑140 (MQ=255) aTTTACCCGGCACGCATTCCAAAAGCCCCTGATTTCTGGCATCCGCAAATGTGGTCCAGGCCGCGCCTTATCACTAATAATCAAACTGTTACCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCA‑ggg > 2:126828/1‑140 (MQ=255) cccGGCACGCATTCCAAAAGCCCCTGATTTCTGGCATCCGCAAATGTGGTCCAGGCCGCGCCTTATCACTAATAATCAAACTGTTACCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCA‑ggggg‑ggg < 2:975906/140‑3 (MQ=255) cccGGCACGCATTCCAAAAGCCCCTGATTTCTGGCATCCGCAAATGTGGTCCAGGCCGCGCCTTATCACTAATAATCAAACTGTTACCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCA‑ggggg‑ggg < 1:798788/140‑3 (MQ=255) cACGCATTCCAAAAGCCCCTGATTTCTGGCATCCGCAAATGTGGTCCAGGCCGCGCCTTATCACTAATAATCAAACTGTTACCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCA‑ggggg‑gggcgttt > 2:527459/1‑133 (MQ=255) ttCCAAAAGCCCCTGATTTCTGGCATCCGCAAATGTGGTCCAGGCCGCGCCTTATCACTAATAATCAAACTGTTACCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAg > 2:554878/1‑140 (MQ=255) ttCCAAAAGCCCCTGATTTCTGGCATCCGCAAATGTGGTCCAGGCCGCGCCTTATCACTAATAATCAAACTGTTACCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAg < 1:97409/140‑1 (MQ=255) ttCCAAAAGCCCCTGATTTCCGGCATCCGCAAATGTGGTCCAGGCCGCGCCTTATCACTAATAATCAAACTGTTACCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAg < 1:86594/140‑1 (MQ=255) cAAAAGCCCCTGATTTCTGGCATCCGCAAATGTGGTCCAGGCCGCGCCTTATCACTAATAATCAAACTGTTACCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCgg < 1:229713/140‑1 (MQ=255) ccccTGATTTCTGGCATCCGCAAATGTGGTCCAGGCCGCGCCTTATCACTAATAATCAAACTGTTACCAATGACGCTCTGGAAATTATCGGAGAAATGCTGCGCATTACCCA‑GGGGGCGGCGGTTTTATAGCGGGCTGGa > 2:453025/1‑140 (MQ=255) cccTGATTTCTGGCATCCGCAAATGCGGTACAGGCCGCGCCTTATCACTAATAATCAAACTGTTACCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGaa < 1:453025/140‑1 (MQ=255) cTGATTTCTGGCATCCGCAAATGTGGTCCAGGCCGCGCCTTATCACTAATAATCAAACTGTTACCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGaaca < 1:126828/140‑1 (MQ=255) cGCAAATGTGGTCCAGGCCGCGCCTTATCACTAATAATCAAACTGTTACCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTg < 1:792901/140‑1 (MQ=255) ttATCACTAATAATCAAACTGTTACCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCAGCttt < 1:781538/140‑1 (MQ=255) aCTAATAATCAAACTGTTACCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCAGCTTTTGCCt < 1:527459/140‑1 (MQ=255) ataatCAAACTGTTACCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCAGCTTTTGCCTGGGa > 2:164484/1‑140 (MQ=255) taatCAAACTGTTACGAATGACGCTCTGGGAATTATCCGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCAGCTTTTGCCTGGGAt < 1:395190/140‑1 (MQ=255) aCTGTTACCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCAGCTTTTGCCTGGGATCTCTTCa > 1:61873/1‑140 (MQ=255) aCTGTTACCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGACAGCTTTTGCCTGGGATCTCTTCa > 2:348423/1‑140 (MQ=255) cTGTTACCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGAACAACTGAAAATGTTCTGCCAGCCACAAACGCTGGCAGCTTTAGCCTGGGATCTCTTCAc > 2:468344/1‑140 (MQ=255) ttACCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCACCTTTTGCCTGGGATCTCTTCACTGc > 1:366629/1‑140 (MQ=255) ttACCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCAACACACGTTGGCAGCTATTGCCTGGGATCTCTTCACTGc > 2:348110/1‑140 (MQ=255) tACCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCAGCTTTTGCCTGGGATCTCTTCACTGCg > 1:733552/1‑140 (MQ=255) aCCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCAGCTTTTGCCTGGGATCTCTTCACTGCGt > 1:3583/1‑140 (MQ=255) aaTGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCAGCTTTTGCCTGGGATCTCTTCACTGCGTGGc < 1:828035/140‑1 (MQ=255) aTGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCAGCTTTTGCCTGGGATCTCTTCACTGc > 2:552794/1‑134 (MQ=255) aTGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCAGCTTTTGCCTGGGATCTCTTCACTGc < 1:552794/134‑1 (MQ=255) gACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGTTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCAGCTTTTGCCTGGGATCTCTTCACTGCGTGGcaac < 1:554878/140‑1 (MQ=255) gACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCAGCTTTTGCCTGGGATCTCTTCACTGCGt > 1:609898/1‑134 (MQ=255) gACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCAGCTTTTGCCTGGGATCTCTTCACTGCGt < 2:609898/134‑1 (MQ=255) cGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCAGCTTTTGCCTGGGATCTCTTCACTGCGTGGc > 1:6962/1‑135 (MQ=255) cGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCAGCTTTTGCCTGGGATCTCTTCACTGCGTGGc < 2:6962/135‑1 (MQ=255) tGGAAATTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GCCGTTTTTATAGCGGCCTGGAACAACTGAAAACGTCCTGCCAGCCACAAACCCTGGCAGCTTTCGGCTTGGATCTCTTCACACCGTGGCAACAAGCTgg > 2:470477/1‑140 (MQ=255) aTTATCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCAGCTTTTGCCTGGGATCTCTTCACTGCGTGGCAACAAGCTGGTGccc < 2:846640/140‑1 (MQ=255) aTCGGCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCAGCTTTTGCCTGGGATCTCTTCACTGCGTGGCAACAAGCTGGTGCCCCCg < 1:83149/140‑1 (MQ=255) gCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCAGCTTTTGCCTGGGATGTCTTCACTGCGCGGGAACAAGATGGTGCCCCCGCaaa > 1:434838/1‑140 (MQ=255) gCGAAATGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCAGCTTTTGCCTGGGATCTCTTCACTGCGTGGCAACAAGCTGGTGCCCCCGCaaa > 1:720244/1‑140 (MQ=255) aaTGCTGCGCTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCAGCTTTTGCCTGGGATCTCTTCACTGCGTGGCAACAAGCTGGTGCCCCCGCAAAAGAc < 2:720244/140‑1 (MQ=255) cgcTTTACCCAGGGGGG‑GGCGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCAGCTTTTGCCTGGGATCTCTTCACTGCGTGGCAACCAGCCGGGGCCCCCGCAAAAGACAACTggg > 1:361571/1‑140 (MQ=255) | CATTTACCCGGCACGCATTCCAAAAGCCCCTGATTTCTGGCATCCGCAAATGTGGTCCAGGCCGCGCCTTATCACTAATAATCAAACTGTTACCAATGACGCTCTGGAAATTATCGGCGAAATGCTGCGCTTTACCCA‑GGGGG‑GACGTTTTTATAGCGGGCTGGAACAACTGAAAACGTTCTGCCAGCCACAAACGCTGGCAGCTTTTGCCTGGGATCTCTTCACTGCGTGGCAACAAGCTGGTGCCCCCGCAAAAGACAACTGGG > NC_000913/2202486‑2202751 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |