| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,734,543 | (C)8→10 | coding (492/732 nt) | pgeF ← | polyphenol oxidase YfiH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,734,535 | 1 | . | C | 93.8% | 104.0 / 0.3 | 32 | coding (500/732 nt) | pgeF | polyphenol oxidase YfiH |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base C (13/17); total (14/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
| * | NC_000913 | 2,734,535 | 2 | . | C | 87.5% | 97.8 / 7.6 | 32 | coding (500/732 nt) | pgeF | polyphenol oxidase YfiH |
| Reads supporting (aligned to +/- strand): ref base . (2/2); new base C (12/16); total (14/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCGCCGAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑‑CCT‑‑‑CCCCCCCC‑‑ACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGC > NC_000913/2734396‑2734662 || gccgccGAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTCGCGTCTACTGCCATAAACGCCTCGCGAA‑‑CCt > 2:427328/1‑140 (MQ=255) gccgAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑‑CCT‑‑‑ccc < 1:668719/140‑1 (MQ=255) aaTTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑‑CCT‑‑‑ccccccccc < 1:998114/140‑2 (MQ=255) aTTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑‑CCT‑‑‑cccccccccc < 1:134606/140‑3 (MQ=255) cGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑‑CCTC‑‑CCCCCCCC‑‑ACTTCGAACGCGCGTGGACCa > 2:9168/1‑140 (MQ=255) gACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑‑CCTC‑‑CCCCCCCC‑‑ACTTCGAACGCGCGTGGACCAATTGc < 1:9168/140‑1 (MQ=255) aCGCTGCCGGGCAAGCTGATAACTAGCCGACAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑‑CCTCC‑CCCCCCCC‑‑ACTTCGAACGAGCGTGGACCAATTGc < 1:480756/140‑1 (MQ=255) aCGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑‑CCTCC‑CCCCCCCC‑‑ACTTCGAACGCGCGTGGACCAATTGc < 1:929006/140‑1 (MQ=255) aCGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑‑CCTCC‑CCCCCCCC‑‑ACTTCGAACGCGCGTGGACCAATTGc < 1:1014720/140‑1 (MQ=255) gCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑‑CCTCC‑CCCCCCCC‑‑ACTTCGAACGCGCGTGGACCAATTGc < 1:997745/135‑1 (MQ=255) gCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑‑CCTCC‑CCCCCCCC‑‑ACTTCGAACGCGCGTGGACCAATTGc > 2:997745/1‑135 (MQ=255) gCTGATAAATATCCGCCAGATACTTATCCCCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑‑CCTCC‑CCCCCCCC‑‑ACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAgg < 1:704498/140‑1 (MQ=255) gATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑‑CCTCC‑CCCCCCCC‑‑ACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCga < 1:550282/140‑1 (MQ=255) aTAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑‑CCTCCCCCCCCCCC‑‑ACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCga < 1:779672/140‑1 (MQ=255) gCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑‑CCTCC‑CCCCCCCC‑‑ACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTcc > 2:886345/1‑140 (MQ=255) gCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑‑CCTCC‑CCCCCCCC‑‑ACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTcc > 2:655367/1‑140 (MQ=255) cATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑‑CCTCC‑CCCCCCCC‑‑ACTTCGAACGCGCGTGGACCATTTGCCGGCCCTATCAACACGAGAATATTTTGAGGATTATCAGCAAAAc > 2:481139/1‑140 (MQ=255) aTGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑‑CCTCC‑CCCCCCCC‑‑ACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACa > 1:59229/1‑140 (MQ=255) tGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑‑CCTCC‑CCCCCCCC‑‑ACTTCGAACGCGCGTGGACCAATGGCCGGCCCAAACGAGGGGAGAATATTTTCCGGATTATCAGCAAAACAg > 2:431920/1‑140 (MQ=255) tGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑‑CCT‑‑‑CCCCCCCCCTTTTTCTAATGCGCGAGGTCCAATGGCGGGCCCGATCGAGGGCAAAAGATTTACCGG‑TTAGCCGCAAAACaag > 2:264490/1‑138 (MQ=255) gCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑‑CCTCC‑CCCCCCCC‑‑ACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAgg > 2:819717/1‑140 (MQ=255) gCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑‑CCTCC‑CCCCCCCC‑‑ACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAgg > 2:672503/1‑140 (MQ=255) gCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑‑CCTCC‑CCCCCCCC‑‑ACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAgg > 2:587861/1‑140 (MQ=255) gaatgaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑‑CCTCC‑CCCCCCCC‑‑ACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGaaa < 1:844389/140‑1 (MQ=255) gaatgaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑‑CCTCC‑CCCCCCCC‑‑ACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGaaa < 1:672503/140‑1 (MQ=255) aatgaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑‑CCTCC‑CCCCCCCC‑‑CCTTCGAACGCGCGTGGACCAATATCCGGCCCTAACCAAGCGAGAATATTTTCCGGAGTAGCAGCAAAACAGGaaaa > 2:743891/1‑139 (MQ=255) gaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑‑CCTCC‑CCCCCCCC‑‑ACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGt < 1:990025/140‑1 (MQ=255) gCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑‑CCTCC‑CCCCCCCC‑‑ACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTctct < 1:886345/140‑1 (MQ=255) aCTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑‑CCTCC‑CCCCCCCC‑‑ACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAg < 2:59229/140‑1 (MQ=255) ttGCTTTAGCGTCTACTGCCATGAATGCCTCGCGAA‑‑CCTCC‑CCCCCCCC‑‑ACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCa < 1:427328/140‑1 (MQ=255) gTCTACTGCCATAACCGCCTCGCGAACCCCC‑‑‑CCCCCCCC‑‑ACGTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca < 1:743891/140‑1 (MQ=255) gTCTACTGCCATAAACGCCTCGCGAA‑‑CCTCC‑CCCCCCCC‑‑ACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca < 1:617469/140‑1 (MQ=255) gTCTACTGCCATAAACGCCTCGCGAA‑‑CCTCC‑CCCCCCCC‑‑ACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca < 1:819717/140‑1 (MQ=255) gTCTACTGCCATAAACGCCTCGCGAA‑‑CCTCC‑CCCCCCCC‑‑ACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca < 1:587861/140‑1 (MQ=255) gTCTACTGCCATAAACGCCTCGCGAA‑‑CCTCC‑CCCCCCCC‑‑ACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca < 1:655367/140‑1 (MQ=255) cTCGCGAA‑‑CCTCC‑CCCCCCCC‑‑ACTTCGAACGCGCGTGGCCCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACCGGAAACCGCCTCATCCCGCACGCCTCCGCCAAGTCCCCGCcagccagc > 2:367583/1‑140 (MQ=255) cTCGCGAA‑‑CCTCC‑CCCCCCCC‑‑ACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCACTTCCAGGACGGCTACGCACAAGCCACGCcagccagc > 1:282005/1‑140 (MQ=255) || GCCGCCGAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑‑CCT‑‑‑CCCCCCCC‑‑ACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGC > NC_000913/2734396‑2734662 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |