| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,970,032 | (G)6→7 | coding (1002/1047 nt) | wzzE → | enterobacterial common antigen polysaccharide co‑polymerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,970,026 | 1 | . | G | 85.7% | 81.5 / 7.5 | 28 | coding (996/1047 nt) | wzzE | enterobacterial common antigen polysaccharide co‑polymerase |
| Reads supporting (aligned to +/- strand): ref base . (2/2); new base G (17/7); total (19/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCC‑GGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑C‑GGG‑GGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTAC > NC_000913/3969889‑3970158 | gCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCC‑GGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑C‑gg < 1:857990/140‑1 (MQ=255)gCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCC‑GGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑C‑gg < 1:769946/140‑1 (MQ=255) gTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCC‑GGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑C‑ggg‑gggg < 2:868656/140‑2 (MQ=255) ccTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCC‑GGAAGAACCGGTAAAACGCGATAGCCCACGTGGTGCCTTCATGATGATTATGTGGGGTATTGG‑CGGGG‑GGG‑CTGATCggg > 2:334970/1‑140 (MQ=255) cTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCC‑GGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGG‑GGG‑CTGATCgggg > 2:941476/1‑140 (MQ=255) tGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCC‑GGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGG‑GGG‑CTGATCGGGGc < 1:852105/140‑1 (MQ=255) tGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCC‑GGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑C‑ggg‑gggg < 1:683203/129‑2 (MQ=255) tGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCC‑GGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑C‑ggg‑gggg > 2:683203/1‑128 (MQ=255) ggTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCC‑GGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGG‑GGG‑CTGATCGGGGCTGGTGTCg > 2:945741/1‑140 (MQ=255) ggTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCC‑GGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGT‑C‑GGGAGGG‑CTGCTCGGGGCTGGTGTCg > 1:408548/1‑140 (MQ=255) gTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCC‑GGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGG‑GGG‑CTGATCGGGGCTGGTGTCGc > 2:704763/1‑140 (MQ=255) cgTTTTCAGACCTATCGTTATTCGCGTACGCG‑GGACGACCCGGTAAACGGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGG‑GGG‑CTGATCGGGGCTGGTGTCGCATTACCCCGCCGTTGCt < 2:345897/140‑1 (MQ=255) cgTTTTCAGACCTATCGCTATTTGCGTACGCC‑GGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑C‑GGG‑GGGCCTGATCGAGGCCGGTGTCGCAATAACCCGCCGTTGCt > 1:439165/1‑140 (MQ=255) tttCAGACCTATCGCTTGTTGCGTACGCC‑GAAAGACCCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGG‑GGG‑CTGATCGGGGCTGGTGTCGCATTACCCCGCCGTTGCTCGa < 2:408548/140‑1 (MQ=255) cTATCGCTATTTGCGTCCGCC‑GGAAGAACGGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTGG‑GGG‑GGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCaa < 2:774096/140‑1 (MQ=255) aTCGCTATTTGCGTACGCC‑GGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGG‑GGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAaca > 1:178607/1‑140 (MQ=255) aTCGCTATTTGCGT‑CGCCGGGAAGATCCTGCGAGAAGCGTTAGCCCTCCTCGTCCTTTTCTGATGTTTATGTGGGGAATTGTGG‑GGG‑GGG‑CTTGCCGGGGCGGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAaca < 2:468950/140‑1 (MQ=255) tCGCTATTTGCGTACGCC‑GGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGG‑GGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAacac > 2:246384/1‑140 (MQ=255) tCGCTATTTGCGTACGCC‑GGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGG‑GGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAacac > 1:152007/1‑140 (MQ=255) ttGCGAGCGCC‑GGAAGACCAGATAAAGCGCGATAGCCCCCGTCTTGCCTTCCTCATGATTTTGTGGGGAATGGTGG‑GGG‑GGG‑CTGATGGGGGGTGGTGTCGCTTTACCCCGCCGTTGCTCGAAATAGCAACACTGCTGCg < 2:206671/140‑1 (MQ=255) cGCC‑GGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGG‑GGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAgcg > 2:681444/1‑140 (MQ=255) ccGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGG‑GGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCAAAATAGCGACAGTGTTGCGGTAAGCGCAAAGGCGCTc > 1:282041/1‑140 (MQ=255) tAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGG‑GGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTcgccg < 1:941476/140‑1 (MQ=255) aaaaCGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGG‑GGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATGGCAACACTGCTACGGTGAGCGGAAAGGCGCTcgccgc > 1:415766/1‑140 (MQ=255) tAGCCAACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGG‑GGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGaa < 1:945741/140‑1 (MQ=255) gtcgtGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGG‑GGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCg > 1:501309/1‑140 (MQ=255) tGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGG‑GGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGAtgt > 1:173845/1‑140 (MQ=255) gCCTTCCTGATGTTTATGTGGGGCTTTGTGG‑GGG‑GGG‑CTGATCGGGGCTGGTGTCGCATTACCCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGAtgtg < 2:501309/140‑1 (MQ=255) ccTTCCTGATGATTATGTGGGGCATTGT‑CGGGG‑GGG‑CTGATAGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGa > 1:621736/1‑140 (MQ=255) ccTGATGATTATGTGGGGCATTGT‑CGGGG‑GGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGt > 1:512595/1‑140 (MQ=255) ttgatgaTTATGTGGGGCATTGTGG‑GGG‑GGG‑GTGAGCGGTGCGGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTa < 2:337973/139‑1 (MQ=255) cTGATGATTATGTGGGGCATTGT‑CGGGG‑GGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTa < 1:704763/140‑1 (MQ=255) taatTATGTGGGGCTTTGG‑CGGGG‑GGG‑TTGTTCGGGGGGGGTGTGCCTTTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactga < 1:984353/138‑1 (MQ=255) tatTATGGGGGGCATTGC‑C‑GGGTGGG‑TGGATCGGGTCTGATGTCGCATTACCCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgac < 2:415766/139‑1 (MQ=255) atTATGTGGGGCATTGT‑CGGGG‑GGG‑CTGATGGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgact < 1:246384/140‑1 (MQ=255) tgtgGGGCATTGT‑CGGGG‑GGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTAt > 1:771996/1‑140 (MQ=255) tgtgGGGCATTGT‑CGGGG‑GGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTAt > 1:548353/1‑140 (MQ=255) cATTGT‑CGGGG‑GGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTAc > 1:595529/1‑140 (MQ=255) | GCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCC‑GGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑C‑GGG‑GGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTAC > NC_000913/3969889‑3970158 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |