| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,606,330 | (G)8→10 | noncoding (72/87 nt) | leuQ ← | tRNA‑Leu |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,606,322 | 1 | . | G | 96.8% | 94.9 / ‑3.1 | 31 | noncoding (80/87 nt) | leuQ | tRNA‑Leu |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (18/12); total (18/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.71e-01 | |||||||||||
| * | NC_000913 | 4,606,322 | 2 | . | G | 83.9% | 78.0 / 11.7 | 31 | noncoding (80/87 nt) | leuQ | tRNA‑Leu |
| Reads supporting (aligned to +/- strand): ref base . (3/2); new base G (15/11); total (18/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.17e-01 | |||||||||||
ATCGCCTCGTTTGGTGCGA‑‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGG‑GG‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAG > NC_000913/4606189‑4606449 || atCGCCTCGTTTGGTGCGAGGGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCACCGCCAACAATATCAACGAGGTTTTTGGAGCGA‑‑ggg‑g > 2:160282/1‑140 (MQ=18) gCCTCGTTTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑ggg‑gg‑ggg < 1:543871/140‑1 (MQ=255) tCGGTTGGTGCGA‑‑GGGGTGGGGGATTGAACCCCCCCGTCCGTAAGAACACTAACCCCTTAAGCCAGCGCGTCTACCAATTCCGCCACCCTCGCCCAGTGCGAGTAATATCAACGTGGTTTTTGGTgggg‑‑ggg‑gg‑ggggga < 2:287510/140‑4 (MQ=18) tGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAG‑GGG‑GG‑GGGACTTGaa < 2:644673/140‑1 (MQ=255) gggggggg‑ggggCCTTGTACCCTCACGTCCGTAAGTACATTAACACCTTAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GG‑GGGACTTGAAcccc < 2:383378/136‑1 (MQ=21) gA‑‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGG‑GG‑GGGACTTGAACCCCCACg < 1:657169/140‑1 (MQ=255) aggggg‑ggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GG‑GGGACTTGAACCCCCa < 1:702359/138‑1 (MQ=255) aCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GG‑GGGACTTGAACCCCCACGTCCGTAAg < 1:86330/140‑1 (MQ=255) gAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GG‑GGGACTTGAACCCCCACGTCCGTAAGGaca > 1:572079/1‑140 (MQ=255) aaCCCCCACGTCCGTAAGAACACTAGCACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCACTATCACCGTGGTTTTTGGCTCGAGCGGG‑GG‑GGGAATTGAACCCCCACGTCCGGAAGGacac > 2:691349/1‑140 (MQ=21) ccACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCCAGCAATAACAACGTGGTTCTTGTTGCGA‑‑GGGCGGTGGTAGTTGAACCCACCCGTCCGTAAGTACACTAtca > 2:396587/1‑140 (MQ=18) cGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATGAACGTGGTTTTTGGTGCGAGGGGG‑GG‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCt < 1:244311/140‑1 (MQ=255) gTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GG‑GGGACTTGAACCCCGACGGCCTTAAGGAAACTAACACCTGAAGc > 1:290877/1‑140 (MQ=37) gTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GG‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGc > 1:106252/1‑140 (MQ=255) tGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAG‑GGG‑GG‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTc > 1:708050/1‑140 (MQ=255) aaGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GG‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc > 1:888708/1‑140 (MQ=255) aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GG‑GGGACTTGAACCCCCCCGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTccc > 1:15663/1‑139 (MQ=25) tAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GG‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa < 1:64826/140‑1 (MQ=255) aGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GG‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAc < 2:888708/140‑1 (MQ=255) cgcgTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGTGAGGGGG‑GG‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt > 2:987902/1‑140 (MQ=255) cgcgTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GG‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt > 2:541866/1‑140 (MQ=255) tCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GG‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGc > 2:136735/1‑140 (MQ=255) cTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GG‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCa > 1:959189/1‑140 (MQ=255) tACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GG‑GGGACTTGAGCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCAt < 2:106252/140‑1 (MQ=255) aCCAATTCCGCCCCCTTCGCCCAGTGGGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GG‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATa < 1:691349/140‑1 (MQ=21) cGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GG‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTaaaaa > 2:720480/1‑140 (MQ=255) ccAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GG‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGaa < 2:572079/140‑1 (MQ=255) gTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GG‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTg > 1:218189/1‑140 (MQ=255) tGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GG‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCCCCACCTTAGCATACCATCAATTCTTAAAAAGAATTGc > 1:453286/1‑140 (MQ=21) aGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GG‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTAcc > 1:760672/1‑140 (MQ=255) atCAACGTGGTTTTTGGTGCGAGGGGG‑GG‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAg > 1:182699/1‑140 (MQ=255) atCAACGTGGTTTTTGGTGCGAG‑GGG‑GG‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAgg > 2:77610/1‑140 (MQ=255) cAACGTGGTTTTTGGTGCGAGGGGG‑GG‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgc > 2:73625/1‑140 (MQ=255) aaCGTGGTTTTTGGTGCGAGGGGG‑GG‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcg < 2:959189/140‑1 (MQ=255) gTGGTTTTTGGTGCGAG‑GGG‑GG‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCCTCGCATACTATCAATTATTAAAAAGGATTGATACCACGGAGGCGCAtt > 2:451030/1‑140 (MQ=18) ttttGGTGCGAGGGGG‑GG‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAg < 2:182699/140‑1 (MQ=255) ttttGGTGCGAGGGGG‑GG‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAg < 1:160282/140‑1 (MQ=255) || ATCGCCTCGTTTGGTGCGA‑‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGG‑GG‑GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAG > NC_000913/4606189‑4606449 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |