Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	686,826	+C	intergenic (‑157/+13)	gltJ ← / ← gltI	glutamate/aspartate ABC transporter membrane subunit GltJ/glutamate/aspartate ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	686,822	1	.	C	100.0%	150.5 / NA	44	intergenic (‑153/+17)	gltJ/gltI	glutamate/aspartate ABC transporter membrane subunit GltJ/glutamate/aspartate ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (23/21);  total (23/21)

AGAGGGTAGCAGCGTTAACTGCTACCCTCGAAGATTGTTACCCA‑‑‑GCGTATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGTATAGTCTGTCCGTGCTACGTAACAATCGAGAGGGCTGGAATTTCCG‑CCCCTGGTTCTTGTAATTAGTTCAGTGCCTTGTCATTCGGTTCTTTGAACAGTGCTTTCATTTCGTCTGACAGTTCGAAATTCATGTTCAGGTTTTTCGGCGGAATTGGATTTTTGAACCACTTATCAAAC  >  NC_000913/686688‑686953                                                                                                                                           |                                                                                                                                    agagGGTAGCAGCGTTAACTGCTACCCTCGAAGATTGTTACCCA‑‑‑GCGTATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGTATAGTCTGTCCGTGCTACGTAACAATCGAGAGGGCTGGAATTTCCG‑ccccc                                                                                                                                >  2:857486/1‑139 (MQ=255)  gagGGTAGCAGCGTTAATTGCTACCCTCGAATATTTTTATCCA‑‑‑GCGTACTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGTATAGTCTGTCCGTGCTACGTAACAATCGAGAGGGCTGGAATTTCCG‑ccccct                                                                                                                               <  2:393310/140‑3 (MQ=255)    gggTAGCAGCGTTAACTGCTACCCTCGAAGATTGTTACCCA‑‑‑GCGTATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGTATAGTCTGTCCGTGCTACGTAACAATCGAGAGGGCTGGAATTTCCG‑ccccctgg                                                                                                                             <  2:1000136/140‑5 (MQ=255)       tAGCAGCGTTAACTGATTCCCTCGAAGATAGTTACCCAGCTGCGTATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGTATAGTCTGTCCGTGCTACGTAACAATCGAGAGGGCTGGAATTTCCG‑ccccctgg                                                                                                                             <  1:405129/140‑5 (MQ=255)         gcagcGTTAACTGCTAATCTCGAAGATTGTTACACG‑‑‑GCGTATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGTATAGTCTGTCCGTGCTACGTAACAATCGAGAGGGCTGGAATTTCCGCCCCCTGGTTCtt                                                                                                                         <  2:469193/140‑1 (MQ=255)              gTTAACTGCTACCCTCGAAGATTGTTACCCA‑‑‑GCGTATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGTATAGTCTGTCCGTGCTACGTAACAATCGAGAGGGCTGGAATTTCCGCCCCCTGGTTCTTGTAAt                                                                                                                    <  1:790433/140‑1 (MQ=255)                 aaCTGCTACCCTCGAAGATTGTTACCCA‑‑‑GCGTATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGTATAGTCTGTCCGTGCTACGTAACAATCGAGAGGGCTGGAATTTCCGCCCCCTGGTTCTTGTAATTAg                                                                                                                 >  2:813958/1‑140 (MQ=255)                    tGCTACCCTCGAAGATTGTTACCCA‑‑‑GCGTATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGTATAGTCTGTCCGTGCTACGTAACAATCGAGAGGGCTGGAATTTCCGCCCCCTGGTTCTTGTAATTAGTTc                                                                                                              <  2:862655/140‑1 (MQ=255)                       tACCCTCGAAGATTGTTACCCA‑‑‑GCGTATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGTATAGTCTGTCCGTGCTACGTAACAATCGAGAGGGCTGGAATTTCCGCCCCCTGGTTCTTGTAATTAGTTCAGt                                                                                                           <  2:55753/140‑1 (MQ=255)                             cGAAGATTGTTACCCA‑‑‑GCGTATGGCGGTTTTTAGGGCCGTTGGGGAACAACCATCAGGCGTATAGTCTGTCCGTGCTACGTAACAATCGAGAGGGCTGGAATTTCCGCCCCCTGGTTCTTGTAATTAGTTCAGTGCCTTg                                                                                                     <  2:434719/140‑1 (MQ=255)                              gAAGATTGTTACCCA‑‑‑GCGTATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGTATAGTCTGTCCGTGCTACGTAACAATCGAGAGGGCTGGAATTTCCGCCCCCTGGTTCTTGTAATTAGTTCAGTGCCTTGt                                                                                                    >  2:593138/1‑140 (MQ=255)                                    tGTTACCCA‑‑‑GCGTATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGTATAGTCTGTCCGTGCTACGTAACAATCGAGAGGGCTGGAATTTCCGCCCCCTGGTTCTTGTAATTAGTTCAGTGCCTTGTCATTCg                                                                                              <  1:857486/140‑1 (MQ=255)                                                 cGTATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGTATAGTCTGTCCGTGCTACGTAACAATCGAGAGGGCTGGAATTTCCGCCCCCTGGTTCTTGTAATTATTTCAGTACCTTGTGATTCGGTTCTTTGaa                                                                                    >  1:471641/1‑140 (MQ=255)                                                 cGTATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGTATAGTCTGTCCGTGCTACGTAACAATCGAGAGGGCTGGAATTTCCGCCCCCTGGTTCTTGTAATTAGTTCAGTGCCTTGTCATTCGGTTCTTTGaa                                                                                    >  1:172927/1‑140 (MQ=255)                                                 cGTATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGTATAGTCTGTCCGTGCTACGTAACAATCGAGAGGGCTGGAATTTCCGCCCCCTGGTTCTTGTAATTAGTTCAGTGCCTTGTCATTCGGTTCTTTGaa                                                                                    >  2:557300/1‑140 (MQ=255)                                                 cGTATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGTATAGTCTGTCCGTGCTACGTAACAATCGAGAGGGCTGGAATTTCCGCCCCCTGGTTCTTGTAATTAGTTCAGTGCCTTGTCATTCGGTTCTTTGaa                                                                                    >  1:201892/1‑140 (MQ=255)                                                  gTATTGCGGTTTTCAGGGCCGATGGGAAACGACCATCAGGCGTATAGTCTGTCCGTGCTACGTAACAATCGAGAGGGCTGGAATTTCCGCCCCCTGGTTCTTGTAATTAGTTCAGTGCCTTGTCTTTCGGTTCTTTGAAc                                                                                   <  2:54504/140‑1 (MQ=255)                                                  gTATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGTATAGTCTGTCCGTGCTACGTAACAATCGAGAGGGCTGGAATTTCCGCCCCCTGGTTCTTGTAATTAGTTCAGTGCCTTGTCATTCGGTTCTTTGAAc                                                                                   <  1:71776/140‑1 (MQ=255)                                                   tATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGTATAGTCTGTCCGTGCTACGTAACAATCGAGAGGGCTGGAATTTCCGCCCCCTGGTTCTTGTAATTAGTTCAGTGCCTTGTCATTCGGTTCTTTGAACa                                                                                  <  1:813958/140‑1 (MQ=255)                                                    aTTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGTATAGTCTGTCCGTGCTACGTAACAATCGAGAGGGCTGGAATTTCCGCCCCCTGGTTCTTGTAATTAGTTCAGTGCCTTGTCATTCGGTTCTTTGATCAg                                                                                 <  2:172927/140‑1 (MQ=255)                                                    aTTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGTATAGTCTGTCCGTGCTACGTAACAATCGAGAGGGCTGGAATTTCCGCCCCCTGGTTCTTGTAATTAGTTCAGTGCCTTGTCATTCGGTTCTTTGAACAg                                                                                 <  2:201892/140‑1 (MQ=255)                                                       gCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGTATAGTCTGTCCGTGCTACGTAACAATCGAGAGGGCTGGAATTTCCGCCCCCTGGTTCTTGTAATTAGTTCAGTGCCTTGTCATTCGGTTCTTTGAACAGTGc                                                                              >  2:15021/1‑140 (MQ=255)                                                            tttCAGGCCCGATGGGGAACGACCATCAGGCGTATAGTCTGTCCGTGCTACGTAACAATCGAGAGGGCTGGAATTTCCGCCCCCTGGTTCTTGTAATTAGTTCAGTGCCTTGTCATTCGGTTCTTTGAACAGTGCTTTCa                                                                         <  1:677375/140‑1 (MQ=255)                                                                       aTGGGGAACGACCATCAGGCGTATAGTCTGTCCGTGCTACGTAACAATCGAGAGGGCTGGAATTTCCGCCCCCTGGTTCTTGTAATTAGTTCAGTGCCTTGTCATTCGGTTCTTTGAACAGTGCTTTCATTTCGTCTGAc                                                              >  1:882141/1‑140 (MQ=255)                                                                       aTGGGGAACGACCATCAGGCGTATAGTCTGTCCGTGCTACGTAACAATCGAGAGGGCTGGAATTTCCGCCCCCTGGTTCTTGTAATTAGTTCAGTGCCTTGTCATTCGGTTCTTTGAACAGTGCTTTCATTTCGTCTGAc                                                              >  2:827220/1‑140 (MQ=255)                                                                         ggggAACGACCATCAGGCGTATAGTCTGTCCGTGCTACGTAACAATCGAGAGGGCTGGAATTTCCGCCCCCTGGTTCTTGTAATTAGTTCAGTGCCTTGTCATTCGGTTCTTTGAACAGTGCTTTCATTTCGTCTGACAg                                                            <  1:70129/140‑1 (MQ=255)                                                                                         gCGTATAGTCTGTCCGTGCTACGTAACAATCGAGAGGGCTGGAATTTCCGCCCCCTGGTTCTTGTAATTAGTTCAGTGCCTTGTCATTCGGTTCTTTGAACAGTGCTTTCATTTCGTCTGACAGTTCGAAATTCATGTTc                                            >  1:523665/1‑140 (MQ=255)                                                                                         gCGTATAGTCTGTCCGTGCTACGTAACAATCGAGAGGGCTGGAATTTCCGCCCCCTGGTTCTTGTAATTAGTTCAGTGCCTTGTCATTCGGTTCTTTGAACAGTGCTTTCATTTCGTCTGACAGTTCGAAATTCATGTTc                                            >  1:523649/1‑140 (MQ=255)                                                                                                 tCTGTCCGTGCTACGTAACAATCGAGAGGGCTGGAATTTCCGCCCCCTGGTTCTTGTAATTAGTTCAGTGCCTTGTCATTCGGTTCTTTGAACAGTGCTTTCATTTCGTCTGACAGTTCGAAATTCATGTTCAGGttttt                                    <  2:882141/140‑1 (MQ=255)                                                                                                 tCTGTCCGTGCTACGTAACAATCGAGAGGGCTGGAATTTCCGCCCCCTGGTTCTTGTAATTAGTTCAGTGCCTTGTCATTCGGTTCTTTGAACAGTGCTTTCATTTCGTCTGACAGTTCGAAATTCATGTTCAGGttttt                                    >  1:617646/1‑140 (MQ=255)                                                                                                   tGTCCGTGCTACGTAACAATCGAGAGGGCTGGAATTTCCGCCCCCTGGTTCTTGTAATTAGTTCAGTGCCTTGTCATTCGGTTCTTTGAACAGTGCTTTCGTTTAGTCAGACAGTTCGAAATTCATGTTCAGGTTTTTcg                                  >  2:334259/1‑140 (MQ=255)                                                                                                   tGTCCGTGCTACGTAACAATCGAGAGGGCTGGAATTTCCGCCCCCTGGTTCTTGTAATTAGTTCAGTGCCTTGTCATTCGGTTCTTTGAACAGTGCTTTCATTTCGTCTGACAGTTCGAAATTCATGTTCAGGTTTTTcg                                  >  1:858069/1‑140 (MQ=255)                                                                                                    gTCCGTGCTACGTAACAATCGAGAGGGCTGGAATTTCCGCCCCCTGGTTCTTGTAATTAGTTCAGTGCCTTGTCATTCGGTTCTTTGAACAGTGCTTTCATTTCGTCTGACAGTTCGAAATTCATGTTCAGGTTTTTcgg                                 <  2:523649/140‑1 (MQ=255)                                                                                                    gTCCGTGCTACGTAACAATCGAGAGGGCTGGAATTTCCGCCCCCTGGTTCTTGTAATTAGTTCAGTGCCTTGTCATTCGGTTCTTTGAACAGTGCTTTCATTTCGTCTGACAGTTCGAAATTCATGTTCAGGTTTTTcgg                                 <  2:523665/140‑1 (MQ=255)                                                                                                        gTGCTACGTAACAATCGAGAGGGCTGGAATTTCCGCCCCCTGGTTCTTGTAATTAGTTCAGTGCCTTGTCATTCGGTTCTTTGAACAGTGCTTTCATTTCGTATGACAGTCCGACATTCATGTTCAGGTTTTTCGGCGGa                             >  1:405550/1‑140 (MQ=255)                                                                                                         tGCTACGTAACAATCGAGAGGGCTGGAATTTCCGCCCCCTGGTTCTTGTAATTAGTTCAGTGCCTTGTCATTCGGTTCTTTGAACAGTGCTTTCATTTCGTCTGACAGTTCGAAATTCATGTTCAGGTTTTTCGGCGGaa                            >  1:7495/1‑140 (MQ=255)                                                                                                           cTACGTAACAATCGAGAGGGCTGGAATTTCCGCCCCCTGGTTCTTGTAATTAGTTCAGTGCCTTGTCATTCGGTTCTTTGAACAGTGCTTTCATTTCGTCTGACAGTTCGAAATTCATGTTCAGGTTTTTCGGCGGAAtt                          >  2:790737/1‑140 (MQ=255)                                                                                                               gTAACAATCGAGAGGGCTGGAATTTCCGCCCCCTGGTTCTTGTAATTAGTTCAGTGCCTTGTCATTCGGTTCTTTGAACAGTGCTTTCATTTCGTCTGACAGGTCGAAATCCATATTAAGGTTTGTCGGCGGAATTGGAt                      >  1:323655/1‑140 (MQ=255)                                                                                                                     aTCGAGAGGGCTGGAATTTCCGCCCCCTGGTTCTTGTAATTAGTTCAGTGCCTTGTCATTCGGTTCTTTGAACAGTGCTTTCATTTCGTCTGACAGTTCGAAATTCATGTTCAGGTTTTTCGGCGGAATTGGATTTTTGa                <  1:593138/140‑1 (MQ=255)                                                                                                                        gagaGGGCTGGAATTTCCGCCCCCTGGTTCTTGTAATTAGTTCAGTGCCTTGTCATTCGGTTCTTTGAACAGTGCTTTCATTTCGTCTGACAGTTCGAAATTCATGTTCAGGTTTTTCGGCGGAATTGGATTTTTGAAcc             <  2:692075/140‑1 (MQ=255)                                                                                                                         agagGGCTGGAATTTCCGCCCCCTGGTTCTTGTAATTAGTTCAGTGCCTTGTCATTCGGTTCTTTGAACAGTGCTTTCATTTCGTCTGACAGTTCGAAATTCATGTTCAGGTTTTTCGGCGGAATTGGATTTTTGAACCa            <  1:827220/140‑1 (MQ=255)                                                                                                                               cTGGAATTTCCGCCCCCTGGTTCTTGTAATTAGTTCAGTGCCTTGTCATTCGGTTCTTTGAACAGTGCTTTCATTTCGTCTGACAGTTCGAAATTCATGTTCAGGTTTTTCGGCGGAATTGGATTTTTg                 >  2:502227/1‑129 (MQ=255)                                                                                                                               cTGGAATTTCCGCCCCCTGGTTCTTGTAATTAGTTCAGTGCCTTGTCATTCGGTTCTTTGAACAGTGCTTTCATTTCGTCTGACAGTTCGAAATTCATGTTCAGGTTTTTCGGCGGAATTGGATTTTTg                 <  1:502227/129‑1 (MQ=255)                                                                                                                                tGGAATTTCCGCCCCCTGGTTCTTGTAATTAGTTCAGTGCCTTGTCATTCGGTTCTTTGAACAGTGCTTTCATTTCGTCTGACAGTTCGAAATTCATGTTCAGGTTTTTCGGCGGAATTGGATTTTTGAACCACTTATCa     >  1:8891/1‑140 (MQ=255)                                                                                                                                tGGAATTTCCGCCCCCTGGTTCTTGTAATTAGTTCAGTGCCTTGTCATTCGGTTCTTTGAACAGTGCTTTCATTTCGTCTGACAGTTCGAAATTCATGTTCAGGTTTTTCGGCGGAATTGGATTTTTGAACCACTTATCa     >  1:917303/1‑140 (MQ=255)                                                                                                                                tGGAATTTCCGCCCCCTGGTTCTTGTAATTAGTTCAGTGCCTTGTCATTCGGTTCTTTGAACAGTGCTTTCATTTCGTCTGACAGTTCGAAATTCATGTTCAGGTTTTTCGGCGGAATTGGATTTTTGAACCACTTATCa     >  1:925130/1‑140 (MQ=255)                                                                                                                                 ggAATTTCCGCCCCCTGGTTCTTGTAATTAGTTCAGTGCCTTGTCATTCGGTTCTTTGAACAGTGCTTTCATTTCGTCTGACAGTTCGAAATTCATGTTCAGGTTTTTCGGCGGAATTGGATTTTTGAACCACTTATCaa    >  1:770240/1‑140 (MQ=255)                                                                                                                                   aaTTTCCGCCCCCTGGTTCTTGTAATTAGTTCAGTGCCTTGTCATTCGGTTCTTTGAACAGTGCTTTCATTTCGTCTGACAGTTCGAAATTCATGTTCAGGTTTTTCGGCGGAATTGGATTTTTGAACCACTTATCAAAc  <  1:557300/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                    AGAGGGTAGCAGCGTTAACTGCTACCCTCGAAGATTGTTACCCA‑‑‑GCGTATTGCGGTTTTCAGGCCCGATGGGGAACGACCATCAGGCGTATAGTCTGTCCGTGCTACGTAACAATCGAGAGGGCTGGAATTTCCG‑CCCCTGGTTCTTGTAATTAGTTCAGTGCCTTGTCATTCGGTTCTTTGAACAGTGCTTTCATTTCGTCTGACAGTTCGAAATTCATGTTCAGGTTTTTCGGCGGAATTGGATTTTTGAACCACTTATCAAAC  >  NC_000913/686688‑686953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑