Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,002,224	(G)7→8	coding (418/1071 nt)	elfG →	putative fimbrial‑like adhesin protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,002,217	1	.	G	100.0%	120.0 / NA	35	T137T (ACA→ACG)	elfG	putative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (23/12);  total (23/12)

GGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑‑GGGG‑GGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCT  >  NC_000913/1002081‑1002332                                                                                                                                          |                                                                                                                      gggCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑‑ggg                                                                                                                   <  2:697482/140‑1 (MQ=255)  ggCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑‑gggg                                                                                                                  <  1:802028/140‑1 (MQ=255)    cATTCGCCGCGGATTCACCGTTAATCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑‑gggg‑gg                                                                                                               <  2:730891/140‑1 (MQ=255)         gccgcGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑‑gggg‑ggggata                                                                                                          >  2:653197/1‑136 (MQ=255)          ccgcGGATTCACCGTTAGTCTATTTAGGCGAGAACAGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑‑gggg‑ggggatac                                                                                                         <  2:328097/140‑6 (MQ=255)            gcgGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAG‑GGGG‑GGGATAccc                                                                                                        >  1:585084/1‑140 (MQ=255)                          aGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAG‑GGGG‑GGGATACCCGATGGCATATTTGc                                                                                          >  1:466213/1‑140 (MQ=255)                          aGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAG‑GGGG‑GGGATACCCGATGGCATATTTGc                                                                                          >  1:818190/1‑140 (MQ=255)                                tttAGGCGACAACTTGCACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAG‑GGGG‑GGGATACCCGATGGCATATTTGCGACCCt                                                                                    >  2:638762/1‑140 (MQ=255)                                      cGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAG‑GGGG‑GGGATACCCGATGGCATATTTGCGACCCTGGCGGt                                                                              <  1:11933/140‑1 (MQ=255)                                      cGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑‑GGGGCGAGATACCCGATAGCATATTTGCGACCCTGGCGGt                                                                              >  2:452475/1‑140 (MQ=255)                                      cGACAACTAGTACAAAACTAATAACTATCTTGCTGCCAAGGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAG‑GGGG‑GGGATACCCGATGGCATATTTGCGACCCTGGCGGt                                                                              <  2:466213/140‑1 (MQ=255)                                          aaCTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAG‑GGGG‑GGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAAc                                                                          >  1:512807/1‑140 (MQ=255)                                                  cAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAG‑GGGG‑GGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGt                                                                  >  1:914147/1‑140 (MQ=255)                                                       ttAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAG‑GGGG‑GGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTCGGCa                                                             >  2:900134/1‑140 (MQ=255)                                                         aaTGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAG‑GGGG‑GGGATACCCGATGGCATATTTGCGACCCTGGCGGTc                                                                             <  2:802852/122‑1 (MQ=255)                                                         aaTGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAG‑GGGG‑GGGATACCCGATGGCATATTTGCGACCCTGGCGGTc                                                                             >  1:802852/1‑122 (MQ=255)                                                         aaTGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAG‑GGGG‑GGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAgg                                                           >  1:940115/1‑140 (MQ=255)                                                              cTATCTTGCCGCCAACGTTTTAGTGAAGGTTAAAGGCGGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAG‑GGGG‑GGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCa                                                      <  1:326284/140‑1 (MQ=255)                                                              cTATCTTGCCGCCAAAGTTTTATTGCAGGTTTAAGACAGCTCTCCTACCGCGGTTCCTTTCGAAAACGTGGGCACAG‑GGGG‑GGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCa                                                      <  2:343593/140‑1 (MQ=255)                                                              cTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAG‑GGGG‑GGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCa                                                      <  2:226059/140‑1 (MQ=255)                                                              cTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAG‑GGGG‑GGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCa                                                      <  2:940115/140‑1 (MQ=255)                                                              cTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAG‑GGGG‑GGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCa                                                      <  2:128436/140‑1 (MQ=255)                                                              cTATCTTGCAGCCAAAGTTTTATTGCAGGCTAAAGGCGGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAG‑GGGG‑GGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCa                                                      <  1:452475/140‑1 (MQ=255)                                                               tATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAG‑GGGG‑GGGATACCCGATGGCATATTTGGGACCCTGTCGGGCAGCGTTTAGGTGGGCAGGGGGCaa                                                     >  1:318241/1‑140 (MQ=255)                                                               tATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAG‑GGGG‑GGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCaa                                                     >  2:172101/1‑140 (MQ=255)                                                                      ccgccAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAG‑GGGG‑GGGATACCCGATGGCATATTTGCGACCCTCGCGGTCACCGTATAAGTGGGCAGGGGGCAAGCGGTaa                                              >  1:343593/1‑140 (MQ=255)                                                                         ccAAAGTTTTATTGCAGATTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAG‑GGGG‑GGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAg                                           >  1:680435/1‑140 (MQ=255)                                                                            aaGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAG‑GGGG‑GGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCgg                                        <  2:585084/140‑1 (MQ=255)                                                                                         ggTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAG‑GGGG‑GGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTg                           >  2:712914/1‑140 (MQ=255)                                                                                                    gTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAG‑GGGG‑GGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCa                >  2:1001272/1‑140 (MQ=255)                                                                                                    gTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAG‑GGGG‑GGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCA‑GGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAg               >  1:243673/1‑140 (MQ=255)                                                                                                     ttCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAG‑GGGG‑GGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGTCAGGGGGCAAGCGGTAGTAGCGGTAGCTTTGCCCTGAAAATATTGCAg               >  2:407000/1‑140 (MQ=255)                                                                                                     ttCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAG‑GGGG‑GGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCt                            >  1:869030/1‑127 (MQ=255)                                                                                                     ttCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAG‑GGGG‑GGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCt                            <  2:869030/127‑1 (MQ=255)                                                                                                     ttCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAG‑GGGG‑GGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAg               >  2:112336/1‑140 (MQ=255)                                                                                                      tctcCTACTGCGGTTCCTTTCGAAAACGTGGGCACAG‑GGGG‑GGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTGGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGc              >  2:1019396/1‑140 (MQ=255)                                                                                                               gCGGTTCCTTTCGAAAACGTGGGCACAG‑GGGG‑GGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGTCGGGGGCAAGCGGGAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTg     >  2:233604/1‑140 (MQ=255)                                                                                                               gCGGTTCCTTTCGAAAACGTGGGCACAG‑GGGG‑GGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGGGGTAGCTTTTCGCTTAAAATAGTGCAGCCGTTCGTTg     >  1:430440/1‑140 (MQ=255)                                                                                                                  gTTCCTTTCGAAAACGTGGGCACAGGGGGG‑GGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGc   >  2:547691/1‑140 (MQ=255)                                                                                                                  gTTCCTTTCGAAAACGTGGGCACAG‑GGGG‑GGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCt  <  1:676512/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                      GGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑‑GGGG‑GGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCT  >  NC_000913/1002081‑1002332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑