Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,865,335	C→T	R101H (CGT→CAT)	yeaE ←	methylglyoxal reductase YeaE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,865,335	0	C	T	98.2%	176.0 / NA	56	R101H (CGT→CAT)	yeaE	methylglyoxal reductase YeaE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (24/31);  minor base G (0/1);  total (24/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATCAAGGTTAGAAACGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCAGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAACCACCTTTTCGGCACCGCCATCGGC  >  NC_000913/1865201‑1865471                                                                                                                                       |                                                                                                                                         aaTCAAGGTTAGAAACGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTATTAAGTAAGGATGGAGATAATCGGTATTGAGATGGCGt                                                                                                                                     >  1:266468/1‑140 (MQ=255)        gTTAGAAACGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCAGTATTTAGATGGCGTAAACTgg                                                                                                                              >  1:515469/1‑140 (MQ=255)        gTTAGAAACGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCAGTATTGAGATGGCGTAAACTgg                                                                                                                              >  1:713398/1‑140 (MQ=255)        gTTAGAAACGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCAGTATTGAGATGGCGTAAACTgg                                                                                                                              >  2:541240/1‑140 (MQ=255)           aGAAACGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATTGAGATAATCAGTATTGAGATGGCGTAAACTGGCtt                                                                                                                           >  2:467675/1‑140 (MQ=255)           aGAAACGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATTGAGAAAATCAGTATTGAGATGGCGTAAACTGGCtt                                                                                                                           >  2:467676/1‑140 (MQ=255)            gAAACGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAATATCCAGAAAATCAGTATTGAGTTGGCGTAAACTGGCTTc                                                                                                                          >  2:406580/1‑140 (MQ=255)             aaaCGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCAGTATTGAGATGGCGTAAACTGGCTTCg                                                                                                                         >  2:43175/1‑140 (MQ=255)             aaaCGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGCCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCAGTATTGAGATTGCGTAAGCTGGCTTCg                                                                                                                         >  1:965498/1‑140 (MQ=255)                cGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCAGTATTGAGATGGCGTAAACTGGCTTCgcat                                                                                                                      <  1:626844/140‑1 (MQ=255)                  ccccAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCAGTATTGAGATGGCGTAAACTGGCtt                                                                                                                           <  1:9541/133‑1 (MQ=255)                  ccccAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCAGTATTGAGATGGCGTAAACTGGCtt                                                                                                                           >  2:9541/1‑133 (MQ=255)                  ccccAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCAGTATTGAGATGGCGTAAACTGGCTTCgcatgc                                                                                                                    >  1:860768/1‑140 (MQ=255)                  ccccAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCAGTATTGAGATGGCGTAAACTGGCTTCgcatgc                                                                                                                    >  1:707985/1‑140 (MQ=255)                        cggcggATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCAGTATTGAGATGGCGTAAACTGGCTTCGCATGCATTTAt                                                                                                              <  1:140543/140‑1 (MQ=255)                        cggcggATTTTTCCCTGGGCGACCAGTTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCAGTATTGAGATGGCGTAAACTGGCTTCACATGCATTTAt                                                                                                              <  1:341727/140‑1 (MQ=255)                         ggcggATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCAGTATTGAGATGGCGTAAACTGGCTTCGCATGCATTTATc                                                                                                             <  1:132181/140‑1 (MQ=255)                                   tCCCTGGGCGATCAATTTTTCCATCGCTGCGCCAGTCTCCTCAAAAGCTAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCAGTATTGAGATGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGcc                                                                                                   <  2:276290/140‑1 (MQ=255)                                      cTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCAGTATTGAGATGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGc                                                                                                <  1:541240/140‑1 (MQ=255)                                      cTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCAGTATTGAGATGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGc                                                                                                <  2:676832/140‑1 (MQ=255)                                        gggCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCAGTATTGAGATGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCa                                                                                              >  1:69751/1‑140 (MQ=255)                                        gggCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCAGTATTGAGATGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCa                                                                                              >  1:526270/1‑140 (MQ=255)                                              tCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCAGTATTGAGATGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTc                                                                                        <  2:69751/140‑1 (MQ=255)                                                aaTTTTTCCATCGCTGCGACAGCCTCTTCAAAAGCGACACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCAGTATTGAGATGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCa                                                                                      <  1:407011/140‑1 (MQ=255)                                                   ttttCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCAGTATTGAGATGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACgg                                                                                   <  1:783737/140‑1 (MQ=255)                                                   ttttCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCAGTATTGAGATGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACgg                                                                                   <  2:707985/140‑1 (MQ=255)                                                    tttCCATCGCTGCGACATTCTCCTCAAAAGCAAAAATGCCAGACCAGTATAATAAGTAAAGATCGAGATAATCAGTATTGAGATGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGa                                                                                  <  2:333568/140‑1 (MQ=255)                                                    tttCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCAGTATTGAGATGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGa                                                                                  <  2:860768/140‑1 (MQ=255)                                                    tttCCATCGCTGCGACAGTCTCCTCAAAAGCGAAACTACCAGACCAGTGTAATAAGTAAAGATCGAGATAATCAGTATTGAGATGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGa                                                                                  <  1:363953/140‑1 (MQ=255)                                                      tCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCAGTATTGAGATGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATa                                                                                <  1:643685/140‑1 (MQ=255)                                                      tCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCAGTATTGAGATGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATa                                                                                <  2:955709/140‑1 (MQ=255)                                                                cGACAGTCTCTTCAATAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCAGTATTGAGATGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTagag                                                                      <  1:406580/140‑1 (MQ=255)                                                                cGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCAGTATTTAGATGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTagag                                                                      <  2:515469/140‑1 (MQ=255)                                                                cGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCAGTATTGAGATGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTagag                                                                      >  1:992595/1‑140 (MQ=255)                                                                cGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCAGTATTGAGATGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTagag                                                                      >  1:95825/1‑140 (MQ=255)                                                                              aaaGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCAGTATTGAGATGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCtt                                                        <  2:681863/140‑1 (MQ=255)                                                                              aaaGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCAGTATTGAGATGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCtt                                                        <  2:95825/140‑1 (MQ=255)                                                                              aaaGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCAGTATTGAGATGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCtt                                                        <  2:992595/140‑1 (MQ=255)                                                                              aaaGCGAAACTGCCACACCAGTCTAATAAGTAAAGATTGAGATAATCAGTATTGAGATGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCtt                                                        <  1:308284/140‑1 (MQ=255)                                                                               aaGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCAGTATTGAGATGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCACTCCACGGATAGAGTTTAAAGACGAGAAAGACCTtc                                                       >  2:308284/1‑140 (MQ=255)                                                                                  cGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCAGTATTGAGATGGCGTAAACTGGCTTTGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTtctct                                                    >  2:563633/1‑140 (MQ=255)                                                                                        tGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCAGTATTGAGATGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGa                                              <  2:106668/140‑1 (MQ=255)                                                                                        tGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCAGTATTGAGATGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGa                                              <  1:147747/140‑1 (MQ=255)                                                                                                  gtgtAATAAGTAAAGCTCGAGAGAAGCAGAATTGAGAGGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATg                                    <  1:467675/140‑1 (MQ=255)                                                                                                  gtgtAATAAGTAAAGCTCGAGACAAGCAGAATTGAGATGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATg                                    <  1:467676/140‑1 (MQ=255)                                                                                                  gtgtAATAAGTAAAGATTGAGAAAATCAGTACTGATATGCCGTAAACTCGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATg                                    <  2:266468/140‑1 (MQ=255)                                                                                                          aGTAAAGATCGAGATAATCAGTATTGAGATGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCa                            <  2:845746/140‑1 (MQ=255)                                                                                                             aaaGATGGAGATAATCAGTATTGAGATGGCGAAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAacc                         <  2:965498/140‑1 (MQ=255)                                                                                                             aaaGATCGAGATAATCAGTATTGAGATGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAacc                         >  1:18856/1‑140 (MQ=255)                                                                                                             aaaGATCGAGATAATCAGTATTGAGATGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGAATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAacc                         >  2:545174/1‑140 (MQ=255)                                                                                                             aaaGATCGAGATAATCAGTATTGAGATGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCC‑CCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAacca                        >  2:951809/1‑140 (MQ=255)                                                                                                                gATCGAGATAATCAGTATTGAGATGGCGTAAACTGGCTTTGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAaccacc                      <  1:563633/140‑1 (MQ=255)                                                                                                                      gaTAATCAGTATTGAGATGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAACCACCTTTTCg                >  1:49691/1‑140 (MQ=255)                                                                                                                         aaTCAGTATTGAGATGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAACCACCTTTTCGGCa             <  2:526270/140‑1 (MQ=255)                                                                                                                           tCAGTATTGAGATGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAACCACCTTTTCGGCAcc           >  2:593317/1‑140 (MQ=255)                                                                                                                                aTTGAGATGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAACCACCTTTTCGGCACCGCCAt      >  1:130881/1‑140 (MQ=255)                                                                                                                                    agaTGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAACCACCTTTTCGGCACCGCCATCGGc  <  1:837109/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         AATCAAGGTTAGAAACGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCAGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAACCACCTTTTCGGCACCGCCATCGGC  >  NC_000913/1865201‑1865471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑