Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,894,717	T→C	L5L (TTA→TTG)	yoaH ←	UFP0181 family protein YoaH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,894,717	0	T	C	100.0%	112.6 / NA	33	L5L (TTA→TTG)	yoaH	UFP0181 family protein YoaH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/25);  total (8/25)

TCGCTCGCCGGAGTGGTTGGCACGCAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCA  >  NC_000913/1894585‑1894848                                                                                                                                     |                                                                                                                                    tcgctcgcCGGAGTGGTTGGCACGCAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGCAAACCTg                                                                                                                              <  2:641250/140‑1 (MQ=255)    ctcgcCGGAGTGGTTGGCACGCAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGCAAACCTGCaa                                                                                                                           <  1:26751/140‑1 (MQ=255)    acagcCGGAGTGGTTCGCACGCTGCCCTTCTGCCACCCGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGCAAACCTGCaa                                                                                                                           <  1:260433/137‑1 (MQ=255)      cgcCGGAGTGGTTGGCACGCAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGCAAACCTGCAAAc                                                                                                                         <  1:755153/140‑1 (MQ=255)       gcCGGAGTGGTTGGCACGCAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGCAAACCTGCAAACa                                                                                                                        <  2:827587/140‑1 (MQ=255)       gcCGGAGTGGTTGGCACGCAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGCAAACCTGCAAACa                                                                                                                        <  1:982788/140‑1 (MQ=255)               ggTTGGCACGCAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGCAAACCTGCAAACATTGTTAAc                                                                                                                <  2:926332/140‑1 (MQ=255)                   ggCACGCAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCGGCTGTTCATGGGAGAGGGAAGGCAAACCTGGAAACATTGTTAAcctgt                                                                                                            >  2:260433/1‑136 (MQ=255)                               ttctGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGCAAACCTGCAAACATTGTTAACTCCTGCTAAAttgttg                                                                                                <  1:244468/140‑1 (MQ=255)                                  tGCCACCAGCGCGATTGCCTGGCCGCTGCTCCTCCCTTGCGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGCAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGgcg                                                                                             <  2:274278/140‑1 (MQ=255)                                  tGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGCAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGgcg                                                                                             <  1:72532/140‑1 (MQ=255)                                          gcgcGATTGCCTGGCCGGTGCTCATCCCTTGTGCCTTCATTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGCAAACCTGCAACCATTGTTAACTCCTGCTAAATTGTTGGCGCTAattat                                                                                     <  2:367366/140‑1 (MQ=255)                                           cgcgATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGCAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAattatt                                                                                    >  1:786041/1‑140 (MQ=255)                                           cgcgATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGCAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAattatt                                                                                    >  2:688056/1‑140 (MQ=255)                                                ttGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGCAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATg                                                                               <  1:656038/140‑1 (MQ=255)                                                ttGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGCAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATg                                                                               <  1:618342/140‑1 (MQ=255)                                                      ggCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGCAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTAccc                                                                         <  2:786041/140‑1 (MQ=255)                                                         cGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGCAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGc                                                                      <  1:747264/140‑1 (MQ=255)                                                                cATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGCAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGc                                                               <  1:844966/140‑1 (MQ=255)                                                                    ccTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGCAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGt                                                           <  1:515893/140‑1 (MQ=255)                                                                    ccTTGTGCCATCAGTTCCTGGACCCGCTCGAAAGCTTTTTGCTGCTGTACATGGGTGAGTGAAGGCAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGt                                                           <  2:347791/140‑1 (MQ=255)                                                                        gtgCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGCAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGTTAATTATATCATGCAACCCGGTACATAGCCAGTagag                                                       >  1:383380/1‑140 (MQ=255)                                                                         tgCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGCAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGt                                                      <  2:934043/140‑1 (MQ=255)                                                                              tCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGCAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGa                                                 >  1:864579/1‑140 (MQ=255)                                                                               cAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGCAAACCTGCAAACATTGTTAACTACTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGAc                                                <  1:998608/140‑1 (MQ=255)                                                                                    ccTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGCAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACtgatg                                           <  2:49029/140‑1 (MQ=255)                                                                                    ccTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGCAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACtgatg                                           <  2:891673/140‑1 (MQ=255)                                                                                          tCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGCAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACg                                     <  1:126122/140‑1 (MQ=255)                                                                                              cTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGCAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTAt                                 >  1:508477/1‑140 (MQ=255)                                                                                                           tgctgcTGTTCATGGGTGAGTGAAGGCAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGAt                    <  2:508477/140‑1 (MQ=255)                                                                                                                      aTGGGTGAGTGAAGGCAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTActct         <  1:71849/140‑1 (MQ=255)                                                                                                                             agtgaAGGCAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCCCCCGCTGTGATTACTTTACTCTGGCGTCa  >  2:891182/1‑140 (MQ=255)                                                                                                                             agtgaAGGCAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGCACTGATGAAGACGTTACCTCCCGCTTTGATTACTTTACTCTGGCGTCa  >  2:364935/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                    TCGCTCGCCGGAGTGGTTGGCACGCAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCA  >  NC_000913/1894585‑1894848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑